Click here to switch to the map view.

The map label for this gene is purR [H]

Identifier: 125623795

GI number: 125623795

Start: 922479

End: 923477

Strand: Reverse

Name: purR [H]

Synonym: llmg_0956

Alternate gene names: 125623795

Gene position: 923477-922479 (Counterclockwise)

Preceding gene: 125623800

Following gene: 125623790

Centisome position: 36.51

GC content: 32.33

Gene sequence:

>999_bases
ATGAAAAAAACATCAATCAAGGATATAGCAAAACTTAGCGGAGTTTCTATTGCGACAGTCTCTCGTGTTATCAATAATAA
TGGACGTTTTTCTGAAGAAACCAGAAAAAAAATTCTTGATGTTATTGAAGAAACTGGCTACCAAATGAATTTTAGTGCCA
AAAGTTTGCGAATGAATAAATCTTTCTCAGTCGGTATTTTAGTTCCAGATATCAGTAATTACTTTTTCTCTGATGTGGTT
CAAAAAATAGAGGAGGAACTTTTTGAATTAGGCTATTCGACAATTATTTGTAATACTGGTAGAAATATTGAAAAAGAAAC
GGCCTACTTAAATATGCTCCGAAGTAAATTGGTTGATGGTATTATCGTTATTTCTGGCGTAGATGAATTTGTCTTTCACT
ATGATGATATTCCATATATTTGTATCGACCGAGAACCCTTGAAAAAAGAGAAAACAATCTTTATTTCTTCAAATCATTAT
CAGGGAGCCTTTGAAGCAACAGAATCATTAATTAATAATGGATGTAAACATCCCGCAATTTTGATGTATGACCGACAATC
TTCTTCTGCAAAAGAGCGTTTTAAAGGATATAAAGATGCGCTAAAGAAAAATAATATTTCCTACAACGAATCAAGTCATC
TGTTATTAAGTGGCCTAGAAGAAAATTTTACTGAAAATTTACAAGCATTTTTAGAGCGAACAGACCAAGTTGATAGTTTT
TTTGCCGTAAATGATACACTTGCCATGAAACTCTTCAGCTCGTTAAAGGAACTAGGGGAGAAAATTCCTGAAGATTTCAA
AATAATTGGGTTTGATGATAGCCCACTCGCTCATTATTCTTCACCAACATTATCATCCGTCAAACAAAACACGGATAAAA
TTGCTCAGCTTACAGTCAAAAATCTAATAGATTTTATTTCCTCTGGGAAGATTATCAATTATCAAACTATTCTAGTACCG
GTTGAATTAGTTGTTAGAAATTCTAGCTTAAATAATTAG

Upstream 100 bases:

>100_bases
CCTGTCAACCCCTCAAATATTATTAGTTATTTGCTAAACTTGTTTATATATGAGAGAATTATATCAATAAAAGATAACGA
TTACTCAAAAGGATAATTCT

Downstream 100 bases:

>100_bases
GAATCAACTCTGACTTTTCTATTAATATTTCTAAAGTACTAATCTATTAAAAAAGTCTACCAGCTTTGGTAGACTTTTTT
AATTTTTCTTATTTATCCTT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKKTSIKDIAKLSGVSIATVSRVINNNGRFSEETRKKILDVIEETGYQMNFSAKSLRMNKSFSVGILVPDISNYFFSDVV
QKIEEELFELGYSTIICNTGRNIEKETAYLNMLRSKLVDGIIVISGVDEFVFHYDDIPYICIDREPLKKEKTIFISSNHY
QGAFEATESLINNGCKHPAILMYDRQSSSAKERFKGYKDALKKNNISYNESSHLLLSGLEENFTENLQAFLERTDQVDSF
FAVNDTLAMKLFSSLKELGEKIPEDFKIIGFDDSPLAHYSSPTLSSVKQNTDKIAQLTVKNLIDFISSGKIINYQTILVP
VELVVRNSSLNN

Sequences:

>Translated_332_residues
MKKTSIKDIAKLSGVSIATVSRVINNNGRFSEETRKKILDVIEETGYQMNFSAKSLRMNKSFSVGILVPDISNYFFSDVV
QKIEEELFELGYSTIICNTGRNIEKETAYLNMLRSKLVDGIIVISGVDEFVFHYDDIPYICIDREPLKKEKTIFISSNHY
QGAFEATESLINNGCKHPAILMYDRQSSSAKERFKGYKDALKKNNISYNESSHLLLSGLEENFTENLQAFLERTDQVDSF
FAVNDTLAMKLFSSLKELGEKIPEDFKIIGFDDSPLAHYSSPTLSSVKQNTDKIAQLTVKNLIDFISSGKIINYQTILVP
VELVVRNSSLNN
>Mature_332_residues
MKKTSIKDIAKLSGVSIATVSRVINNNGRFSEETRKKILDVIEETGYQMNFSAKSLRMNKSFSVGILVPDISNYFFSDVV
QKIEEELFELGYSTIICNTGRNIEKETAYLNMLRSKLVDGIIVISGVDEFVFHYDDIPYICIDREPLKKEKTIFISSNHY
QGAFEATESLINNGCKHPAILMYDRQSSSAKERFKGYKDALKKNNISYNESSHLLLSGLEENFTENLQAFLERTDQVDSF
FAVNDTLAMKLFSSLKELGEKIPEDFKIIGFDDSPLAHYSSPTLSSVKQNTDKIAQLTVKNLIDFISSGKIINYQTILVP
VELVVRNSSLNN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=28.4848484848485, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=28.1437125748503, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=30.8823529411765, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788474, Length=303, Percent_Identity=29.7029702970297, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790369, Length=329, Percent_Identity=24.6200607902736, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=29.553264604811, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=25.9587020648968, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=26.1980830670927, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787906, Length=304, Percent_Identity=25.6578947368421, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=25.5591054313099, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI48994940, Length=323, Percent_Identity=26.3157894736842, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786268, Length=301, Percent_Identity=24.5847176079734, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790689, Length=328, Percent_Identity=22.8658536585366, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789456, Length=348, Percent_Identity=23.2758620689655, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37547; Mature: 37547

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTSIKDIAKLSGVSIATVSRVINNNGRFSEETRKKILDVIEETGYQMNFSAKSLRMNK
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEECCHHHHCCCC
SFSVGILVPDISNYFFSDVVQKIEEELFELGYSTIICNTGRNIEKETAYLNMLRSKLVDG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
IIVISGVDEFVFHYDDIPYICIDREPLKKEKTIFISSNHYQGAFEATESLINNGCKHPAI
EEEEECHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEE
LMYDRQSSSAKERFKGYKDALKKNNISYNESSHLLLSGLEENFTENLQAFLERTDQVDSF
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
FAVNDTLAMKLFSSLKELGEKIPEDFKIIGFDDSPLAHYSSPTLSSVKQNTDKIAQLTVK
EEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NLIDFISSGKIINYQTILVPVELVVRNSSLNN
HHHHHHHCCCEEEEEEEEEEHHHEEECCCCCC
>Mature Secondary Structure
MKKTSIKDIAKLSGVSIATVSRVINNNGRFSEETRKKILDVIEETGYQMNFSAKSLRMNK
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEECCHHHHCCCC
SFSVGILVPDISNYFFSDVVQKIEEELFELGYSTIICNTGRNIEKETAYLNMLRSKLVDG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
IIVISGVDEFVFHYDDIPYICIDREPLKKEKTIFISSNHYQGAFEATESLINNGCKHPAI
EEEEECHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEE
LMYDRQSSSAKERFKGYKDALKKNNISYNESSHLLLSGLEENFTENLQAFLERTDQVDSF
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
FAVNDTLAMKLFSSLKELGEKIPEDFKIIGFDDSPLAHYSSPTLSSVKQNTDKIAQLTVK
EEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NLIDFISSGKIINYQTILVPVELVVRNSSLNN
HHHHHHHCCCEEEEEEEEEEHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA