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The map label for this gene is papL

Identifier: 125623783

GI number: 125623783

Start: 909616

End: 910824

Strand: Direct

Name: papL

Synonym: llmg_0942

Alternate gene names: 125623783

Gene position: 909616-910824 (Clockwise)

Preceding gene: 125623782

Following gene: 125623784

Centisome position: 35.96

GC content: 35.9

Gene sequence:

>1209_bases
ATGAAATTAGAAAAATTACCCGAGGAGTTTGTACAGGCTCAGCCGGTTTTAGAAAAAATAAGCGAGCATGGTTTTGAAGC
TTATTTTGTTGGAGGTTCTGTACGGGATGTATTGCTTGGGCGAGAGATTCACGATGTAGATATTGCAACGAGTGCTTACC
CGGAGGAGATAAAAGATATTTTTCCTTATACTATCGATGTCGGTATTGAGCATGGAACTGTTCTTGTTTTAGCTGGAAAA
TCGGAAGCCGAGCATTATGAAATTACTACTTTTCGGACAGAATCAAAATATACAGATTACAGACGACCTGACCATGTGGA
TTTCGTCAGAGATTTACGTGAGGATTTAAAACGGCGCGACTTTACGGTTAATGCTTTTGCCTGTGATTTTGAGGGTCAAA
TCATTGATTTATTTGATGGTTTAACAGATTTAAAAGAACGAAGATTGACAGCTGTTGGGAGCGCTTTGGAGCGTTTCAAT
GAAGATGCCTTACGGATTATGCGTGCCATGCGTTTTGCTTCCACTTTGGATTTTAAAATTGAAGAGAAAACTTTTTCAGC
CATGCGTGAGCGTTCTCACCTCTTAGAAAAAATTTCGGTTGAGCGAATATTTATCGAATTAGATAAATTATTGTTGGGGA
GTGAATGGCGCAATGGTCTGACTTTATTGATTGAATCAGAAGCGTGGAAATACTTACCAGATTTTCAAGATTTAGCCTTA
AAAAAAGTGCTGACAGAGCTGTCAGTAGATTTTCACTTTAAAAATTCAGAACAAGCATGGGCAGCCTTACTGACTAGATT
TTCAAATATTGATGTGAAGGCTTTTTTGCGCAAATGGAAAGTATCAAATGAATTTTCAAAATATGTAGCTGACCTTGTTT
CAGCCTATGAACTTGAAAATTGGGATTTGGTCAGTCTTTATCATTTTGGTTTGGAAAAAGCACTTCTGGTTGATGAGTTG
AAAATTGCTTTTGGAAGGGATATTGACAGAGAACGAGCAGTTTTTATTAATGACCAATTGCAAATTCATGATAAATCAGA
AATTGTTATTGCTGGAAAAGATTTGATGGATGAATTTTCCCTTAAGCCCGGACCAGAGTTGGGAAAAATATTAAAAACTA
TTGAAGAAAAAATAGTTAAAAATGAGTTGAAAAATGAGCAAATTGCAATTTTAACAGAAGTTAAAAAAATGCTTGAACTT
GAAAAATAA

Upstream 100 bases:

>100_bases
GGTCAGTATTTTAATAGTATTCAAAAATTCTGTCAGTAAAATTGGAAGCCTATAATGGGGCTATGCCCCTTTTCCTATAA
AAAATGATAAAATAAAAGAT

Downstream 100 bases:

>100_bases
AAGTAAACAAATTTTACTGATAGAAATTCTGAAAAAAATCAATTAATATTGTATTTCTGCTAGAAACACAATTGTTGATA
TTTTTGTCAATGTATTCTAT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKLEKLPEEFVQAQPVLEKISEHGFEAYFVGGSVRDVLLGREIHDVDIATSAYPEEIKDIFPYTIDVGIEHGTVLVLAGK
SEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRDFTVNAFACDFEGQIIDLFDGLTDLKERRLTAVGSALERFN
EDALRIMRAMRFASTLDFKIEEKTFSAMRERSHLLEKISVERIFIELDKLLLGSEWRNGLTLLIESEAWKYLPDFQDLAL
KKVLTELSVDFHFKNSEQAWAALLTRFSNIDVKAFLRKWKVSNEFSKYVADLVSAYELENWDLVSLYHFGLEKALLVDEL
KIAFGRDIDRERAVFINDQLQIHDKSEIVIAGKDLMDEFSLKPGPELGKILKTIEEKIVKNELKNEQIAILTEVKKMLEL
EK

Sequences:

>Translated_402_residues
MKLEKLPEEFVQAQPVLEKISEHGFEAYFVGGSVRDVLLGREIHDVDIATSAYPEEIKDIFPYTIDVGIEHGTVLVLAGK
SEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRDFTVNAFACDFEGQIIDLFDGLTDLKERRLTAVGSALERFN
EDALRIMRAMRFASTLDFKIEEKTFSAMRERSHLLEKISVERIFIELDKLLLGSEWRNGLTLLIESEAWKYLPDFQDLAL
KKVLTELSVDFHFKNSEQAWAALLTRFSNIDVKAFLRKWKVSNEFSKYVADLVSAYELENWDLVSLYHFGLEKALLVDEL
KIAFGRDIDRERAVFINDQLQIHDKSEIVIAGKDLMDEFSLKPGPELGKILKTIEEKIVKNELKNEQIAILTEVKKMLEL
EK
>Mature_402_residues
MKLEKLPEEFVQAQPVLEKISEHGFEAYFVGGSVRDVLLGREIHDVDIATSAYPEEIKDIFPYTIDVGIEHGTVLVLAGK
SEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRDFTVNAFACDFEGQIIDLFDGLTDLKERRLTAVGSALERFN
EDALRIMRAMRFASTLDFKIEEKTFSAMRERSHLLEKISVERIFIELDKLLLGSEWRNGLTLLIESEAWKYLPDFQDLAL
KKVLTELSVDFHFKNSEQAWAALLTRFSNIDVKAFLRKWKVSNEFSKYVADLVSAYELENWDLVSLYHFGLEKALLVDEL
KIAFGRDIDRERAVFINDQLQIHDKSEIVIAGKDLMDEFSLKPGPELGKILKTIEEKIVKNELKNEQIAILTEVKKMLEL
EK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=215, Percent_Identity=34.8837209302326, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI87081691, Length=223, Percent_Identity=33.1838565022422, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1789436, Length=197, Percent_Identity=37.0558375634518, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71995920, Length=220, Percent_Identity=35, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321016, Length=209, Percent_Identity=31.5789473684211, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281360112, Length=201, Percent_Identity=35.8208955223881, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357337, Length=201, Percent_Identity=35.8208955223881, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_LACLM (A2RJT1)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032266.1
- ProteinModelPortal:   A2RJT1
- SMR:   A2RJT1
- STRING:   A2RJT1
- GeneID:   4796595
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_0942
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GKASERF
- ProtClustDB:   PRK13299
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46654; Mature: 46654

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 35-35 BINDING 119-119 BINDING 162-162 BINDING 165-165 BINDING 168-168 BINDING 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEKLPEEFVQAQPVLEKISEHGFEAYFVGGSVRDVLLGREIHDVDIATSAYPEEIKDI
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
FPYTIDVGIEHGTVLVLAGKSEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRD
CCEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
FTVNAFACDFEGQIIDLFDGLTDLKERRLTAVGSALERFNEDALRIMRAMRFASTLDFKI
CEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEE
EEKTFSAMRERSHLLEKISVERIFIELDKLLLGSEWRNGLTLLIESEAWKYLPDFQDLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCHHHHHH
KKVLTELSVDFHFKNSEQAWAALLTRFSNIDVKAFLRKWKVSNEFSKYVADLVSAYELEN
HHHHHHHHHCEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
WDLVSLYHFGLEKALLVDELKIAFGRDIDRERAVFINDQLQIHDKSEIVIAGKDLMDEFS
CCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEEECCCCEEEECHHHHHHCC
LKPGPELGKILKTIEEKIVKNELKNEQIAILTEVKKMLELEK
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLEKLPEEFVQAQPVLEKISEHGFEAYFVGGSVRDVLLGREIHDVDIATSAYPEEIKDI
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
FPYTIDVGIEHGTVLVLAGKSEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRD
CCEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
FTVNAFACDFEGQIIDLFDGLTDLKERRLTAVGSALERFNEDALRIMRAMRFASTLDFKI
CEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEE
EEKTFSAMRERSHLLEKISVERIFIELDKLLLGSEWRNGLTLLIESEAWKYLPDFQDLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCHHHHHH
KKVLTELSVDFHFKNSEQAWAALLTRFSNIDVKAFLRKWKVSNEFSKYVADLVSAYELEN
HHHHHHHHHCEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
WDLVSLYHFGLEKALLVDELKIAFGRDIDRERAVFINDQLQIHDKSEIVIAGKDLMDEFS
CCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEEECCCCEEEECHHHHHHCC
LKPGPELGKILKTIEEKIVKNELKNEQIAILTEVKKMLELEK
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA