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The map label for this gene is ubiE

Identifier: 125623607

GI number: 125623607

Start: 742828

End: 743568

Strand: Direct

Name: ubiE

Synonym: llmg_0753

Alternate gene names: 125623607

Gene position: 742828-743568 (Clockwise)

Preceding gene: 125623606

Following gene: 125623608

Centisome position: 29.37

GC content: 34.14

Gene sequence:

>741_bases
ATGACTAAAGTAAACGAAGAACGTGTGCAAGAAATCTTTAATAGTATTTCATCAGATTATGATAAAATGAACGCCATTAT
CAGTTTTAAACAGCATGATTTATGGCGGGCTAAAACAATGAAAAGAATGGGAGATTTAACTGGTCTATCTATTCTTGATT
TGTGTTGTGGAACTGGAGACTGGACTTTTGATTTATCTGAAAGTGTTGGCCCTTCTGGTAAAGTAATTGGTCTTGATTTT
TCAGAAAATATGCTCGAAATTGCTAAGGCTAAACTTAAAGAAGAAGCGAAGAAAAACATTGAATTTCTACAAGGAAATGC
GATGGCTTTACCTTTTGAAAAAGGAAGCTTTGATGTAGTTACGATTGGTTATGGGCTAAGAAACACACCTGACTATCTTA
CTGTATTAAAAGAAATTTTTCGTGTCTTAAAACCAGGGGGCAGAGTAGTTTGTATCGAAACTTCACATCCCACATTGCCA
ATTTATAAACAAGCTTTTGAACTTTATTTTAAAAATGTCATGCCCTTTTTGGGTAAAGTTTTTGCCAAATCTTTAAAAGA
ATATCAATGGCTTCAAAAATCAGCAGAAGATTTTCCAGATGCCAAGACGCTTGAAGAACTTTTTAGAAAAGCTGGCTTTG
TTGCCGTAGAATATCAAAAACATGGTGGTGGAGCAATCGCAAGTCATTTTGCCACTAAATCCCAAAAACCTAAATCAAAT
ATTCGTATTGGTAAAAAGTAA

Upstream 100 bases:

>100_bases
AAAATATAAAAAATTGCTGACCTTGTGACCAGTAATTTTTTTATATTTTATCAATCATAATCAGTAAATAGTAGCATAGA
AGTTGTGCTATAATAAGTCT

Downstream 100 bases:

>100_bases
TCGGTTAATAAAAAAGAAAAATTTCGAGGAAAAAATATGCCTTATAAACGTTATGGGGAAATTTTTAAAAAACTACGAGA
ACAAAAGAATTTTTCGCTTT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: gerC2 protein homolog

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTKVNEERVQEIFNSISSDYDKMNAIISFKQHDLWRAKTMKRMGDLTGLSILDLCCGTGDWTFDLSESVGPSGKVIGLDF
SENMLEIAKAKLKEEAKKNIEFLQGNAMALPFEKGSFDVVTIGYGLRNTPDYLTVLKEIFRVLKPGGRVVCIETSHPTLP
IYKQAFELYFKNVMPFLGKVFAKSLKEYQWLQKSAEDFPDAKTLEELFRKAGFVAVEYQKHGGGAIASHFATKSQKPKSN
IRIGKK

Sequences:

>Translated_246_residues
MTKVNEERVQEIFNSISSDYDKMNAIISFKQHDLWRAKTMKRMGDLTGLSILDLCCGTGDWTFDLSESVGPSGKVIGLDF
SENMLEIAKAKLKEEAKKNIEFLQGNAMALPFEKGSFDVVTIGYGLRNTPDYLTVLKEIFRVLKPGGRVVCIETSHPTLP
IYKQAFELYFKNVMPFLGKVFAKSLKEYQWLQKSAEDFPDAKTLEELFRKAGFVAVEYQKHGGGAIASHFATKSQKPKSN
IRIGKK
>Mature_245_residues
TKVNEERVQEIFNSISSDYDKMNAIISFKQHDLWRAKTMKRMGDLTGLSILDLCCGTGDWTFDLSESVGPSGKVIGLDFS
ENMLEIAKAKLKEEAKKNIEFLQGNAMALPFEKGSFDVVTIGYGLRNTPDYLTVLKEIFRVLKPGGRVVCIETSHPTLPI
YKQAFELYFKNVMPFLGKVFAKSLKEYQWLQKSAEDFPDAKTLEELFRKAGFVAVEYQKHGGGAIASHFATKSQKPKSNI
RIGKK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=257, Percent_Identity=31.5175097276265, Blast_Score=135, Evalue=2e-32,
Organism=Escherichia coli, GI48994983, Length=224, Percent_Identity=38.8392857142857, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI1786404, Length=130, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17557039, Length=234, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17561380, Length=114, Percent_Identity=34.2105263157895, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323526, Length=241, Percent_Identity=31.5352697095436, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24641702, Length=238, Percent_Identity=30.672268907563, Blast_Score=134, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_LACLA (P49016)

Other databases:

- EMBL:   L14679
- EMBL:   AE005176
- PIR:   B48653
- ProteinModelPortal:   P49016
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 27722; Mature: 27591

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVNEERVQEIFNSISSDYDKMNAIISFKQHDLWRAKTMKRMGDLTGLSILDLCCGTGD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
WTFDLSESVGPSGKVIGLDFSENMLEIAKAKLKEEAKKNIEFLQGNAMALPFEKGSFDVV
CEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
TIGYGLRNTPDYLTVLKEIFRVLKPGGRVVCIETSHPTLPIYKQAFELYFKNVMPFLGKV
EECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FAKSLKEYQWLQKSAEDFPDAKTLEELFRKAGFVAVEYQKHGGGAIASHFATKSQKPKSN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCC
IRIGKK
CCCCCC
>Mature Secondary Structure 
TKVNEERVQEIFNSISSDYDKMNAIISFKQHDLWRAKTMKRMGDLTGLSILDLCCGTGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
WTFDLSESVGPSGKVIGLDFSENMLEIAKAKLKEEAKKNIEFLQGNAMALPFEKGSFDVV
CEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
TIGYGLRNTPDYLTVLKEIFRVLKPGGRVVCIETSHPTLPIYKQAFELYFKNVMPFLGKV
EECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FAKSLKEYQWLQKSAEDFPDAKTLEELFRKAGFVAVEYQKHGGGAIASHFATKSQKPKSN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCC
IRIGKK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366036; 11337471