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The map label for this gene is brnQ [H]

Identifier: 125623510

GI number: 125623510

Start: 645202

End: 646551

Strand: Reverse

Name: brnQ [H]

Synonym: llmg_0650

Alternate gene names: 125623510

Gene position: 646551-645202 (Counterclockwise)

Preceding gene: 125623511

Following gene: 125623508

Centisome position: 25.56

GC content: 36.3

Gene sequence:

>1350_bases
ATGAAGGAGAAACTAGCTGGTAAAGACTATCTTTTTATTGGATCAATGTTATTTGGACTTTTTTTCGGTGCTGGAAATTT
AATTTTCCCTATCCATATGGGTCAAGAAGCAGGTGATGCTATCTCTCAAGCCAATTTTGGTTTTTTAGTTACAGCAGTTG
GATTTCCATTTTTAGGAATCATTGCATTAGGAATATCACAAAGTAATGGCGTCTTTGAATTGGCTACCCGAGTCAATCGT
ATTTATGCCTATATCTTTACGATTTTACTTTATTTGGTCATTGGTCCCTTTTTTGCACTGCCACGTCTAGCAACAACTTC
GTTCGAAATTGGAATTTCTCCATTTTTATCTAACAAACTTCAAACCCCACTTTTAGCACTCTTTAGTATCCTATTCTTTG
GCACTGCTTGGTTTTTATCTAGAAAACCCACAAAACTATTGGATTATATTGGAAAATTTTTAAATCCACTTTTTCTAGTC
TTGTTAGGTTTAATCTTAATCATCGCTTTTAGTCACCCTCTTGGATCCGTTCACCAAGCAGAGGTTGGTAAACTTTATCA
AAGTTCTGCTTTTATGAATGGTTTTACTCAGGGCTACAATACACTTGACGCATTAGCAGCCTTAGCTTTCGGAATTATTA
TTATCACAACGATTCGTCAGCGGGGAGTTAAAAATTCCAAAATTATTGCTAAGGAAACCATTAAAGCCGGTTTGATTAGC
GTAGGACTTATGGCTATAATTTATACTTGCCTCTCTTACTTAGGAGCCATGAGCGTAGGAAAATTTGCGATAAGTGAAAA
TGGTGGAATTGCCCTTGCCCAAATTTCCAATTACTATCTTGGAACTTTTGGAATGATTATCTTGGCTTTGATTGTTATCA
TTGCCTGTTTAAAAACTGCTGTTGGGTTGATGTCTGCCTTCTCTGAAACCTTCGTAGAATTATTTCCAAAAAGAGAATAT
CGCTTTTACTTAATGATCGTTAGTATCCTCCCTTGTATTTTTGCAAATATCGGTTTAACAAAAATCATAGAACTTTCTGT
TCCAGTGCTTATGTTTTTATATCCATTAGCAATCACACTTATCCTTTTAGCCTTATTAGGTCCAGTTTTTCAACATTCTA
AACTTGTCTATCAAATAACCACCGCCTTCACACTTTTGGCTGCTATCGCCGACGGACTCAATGCACTTCCAAATCCGCTA
AAATCACTTAAGCCTATCGCAGCCATTCTCAATTTTTCCAAAGACTCTCTCCCCTTCTTCTCTCTTGGAATGGGCTGGAT
TCTCCTAGCAGTCCTTGGATTTATAATCGGTTGTTTAGTCCACTTTTTAAAAAGAATCACAAATAAATAA

Upstream 100 bases:

>100_bases
TTAAAAATTCTTAAATTGTGTTGTAAGTTCACAATTATTTTGCTATAATGACTTATTATTTTATTCACAAAATAGAAAAT
GAAATTATTAGGGAGAACTT

Downstream 100 bases:

>100_bases
AAGAGCTTCTAATATTTAGGAGCTCTTTTATTTATTTTTAAAGACCGCACTTCTAAGAATTTATTTTATATTTAGTGATG
TATCTTTCATTTCTTTACAA

Product: branched chain amino acid ABC transporter carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKEKLAGKDYLFIGSMLFGLFFGAGNLIFPIHMGQEAGDAISQANFGFLVTAVGFPFLGIIALGISQSNGVFELATRVNR
IYAYIFTILLYLVIGPFFALPRLATTSFEIGISPFLSNKLQTPLLALFSILFFGTAWFLSRKPTKLLDYIGKFLNPLFLV
LLGLILIIAFSHPLGSVHQAEVGKLYQSSAFMNGFTQGYNTLDALAALAFGIIIITTIRQRGVKNSKIIAKETIKAGLIS
VGLMAIIYTCLSYLGAMSVGKFAISENGGIALAQISNYYLGTFGMIILALIVIIACLKTAVGLMSAFSETFVELFPKREY
RFYLMIVSILPCIFANIGLTKIIELSVPVLMFLYPLAITLILLALLGPVFQHSKLVYQITTAFTLLAAIADGLNALPNPL
KSLKPIAAILNFSKDSLPFFSLGMGWILLAVLGFIIGCLVHFLKRITNK

Sequences:

>Translated_449_residues
MKEKLAGKDYLFIGSMLFGLFFGAGNLIFPIHMGQEAGDAISQANFGFLVTAVGFPFLGIIALGISQSNGVFELATRVNR
IYAYIFTILLYLVIGPFFALPRLATTSFEIGISPFLSNKLQTPLLALFSILFFGTAWFLSRKPTKLLDYIGKFLNPLFLV
LLGLILIIAFSHPLGSVHQAEVGKLYQSSAFMNGFTQGYNTLDALAALAFGIIIITTIRQRGVKNSKIIAKETIKAGLIS
VGLMAIIYTCLSYLGAMSVGKFAISENGGIALAQISNYYLGTFGMIILALIVIIACLKTAVGLMSAFSETFVELFPKREY
RFYLMIVSILPCIFANIGLTKIIELSVPVLMFLYPLAITLILLALLGPVFQHSKLVYQITTAFTLLAAIADGLNALPNPL
KSLKPIAAILNFSKDSLPFFSLGMGWILLAVLGFIIGCLVHFLKRITNK
>Mature_449_residues
MKEKLAGKDYLFIGSMLFGLFFGAGNLIFPIHMGQEAGDAISQANFGFLVTAVGFPFLGIIALGISQSNGVFELATRVNR
IYAYIFTILLYLVIGPFFALPRLATTSFEIGISPFLSNKLQTPLLALFSILFFGTAWFLSRKPTKLLDYIGKFLNPLFLV
LLGLILIIAFSHPLGSVHQAEVGKLYQSSAFMNGFTQGYNTLDALAALAFGIIIITTIRQRGVKNSKIIAKETIKAGLIS
VGLMAIIYTCLSYLGAMSVGKFAISENGGIALAQISNYYLGTFGMIILALIVIIACLKTAVGLMSAFSETFVELFPKREY
RFYLMIVSILPCIFANIGLTKIIELSVPVLMFLYPLAITLILLALLGPVFQHSKLVYQITTAFTLLAAIADGLNALPNPL
KSLKPIAAILNFSKDSLPFFSLGMGWILLAVLGFIIGCLVHFLKRITNK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=432, Percent_Identity=36.1111111111111, Blast_Score=251, Evalue=9e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48901; Mature: 48901

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8544834 [H]