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The map label for this gene is pmrA [H]

Identifier: 125623491

GI number: 125623491

Start: 621618

End: 622835

Strand: Direct

Name: pmrA [H]

Synonym: llmg_0631

Alternate gene names: 125623491

Gene position: 621618-622835 (Clockwise)

Preceding gene: 125623490

Following gene: 125623492

Centisome position: 24.57

GC content: 38.59

Gene sequence:

>1218_bases
GTGAATCACACGAAAACAGTTAATTGGAAAAGGAATTTATTTATAACATGGATTGGCTGCTTTTTTGTAGGTTCTTCATT
TTCACTAGTGATGCCCTTTTTGCCTTTGTACATACAAGGATTGGGCGTAAGTGGTGGGAATGTTGAACTTTACTCAGGGT
TAGCTTTTTCTTTGCCAGCATTAGCTTCTGGTCTTGTTGCCCCGATTTGGGGACGTTTAGCTGATGAACATGGTCGTAAA
GTGATGATGGTAAGAGCTAGTATCGTTATGACTTTGACCATGGGAGGAATTGCATTTGTTCCCAATGTTTGGTGGTTACT
TGGCCTTCGCCTACTGATGGGATTTTTCTCAGGCTATATTCCTAACTCGACTGCCATGATTGCCTCACAAGCTCCAAAAG
ATAAATCAGGCTATGCACTTGGAACCTTAGCTACTGCAATGGTTTCTGGAACATTGATTGGGCCATCACTTGGTGGATTA
TTCGCCGAGTGGTTTGGAATGGCAAATGTCTTTTTGATTGTAGGGGCTTTATTAGCACTAGCAACTTTACTGACTATCTT
TTTTGTTCATGAAAACTTTGAGCCGATTGCAAAAGGTGAAATGTTGTCTAGCAGGGAAATTATCAATAAAGTAAGTAATA
AACAAATTCTTTTTGGTTTATTGGTTACAACATTTATTATTCAAATCACCTCACAATCTATTGAACCATTTGTCACTTTA
TATATTAAAACGCTGACAACAAGCACTAATAATCTGATGTTTATTTCAGGTCTAATTGTCTCTGCAGTTGGCTTATCAGC
GATGATGTCCTCAAGTTTTTTGGGGCGTTTAGGTGATAAATATGGATCACATCGATTGATCTTGATTGGTTTGGTTTTCA
CATTTGTTATTTACTTGCCAATGGCTTTTGTGCAAAGTCCATTACAACTTGGAATTTTAAGATTCCTTCTTGGCTTTGGT
GCGGGGGCTTTGATGCCATCAGTAAATTCTCTACTTTCAAAAATTACGCCAAAAGAAGGAGTTAGTCGAATTTTTGCTTA
TGCACAAATGTGCTCAAATCTCGGAATGGTCACCGGTCCCTTGGTTGGTTCAGCCATTGCTGGGTACATTAGTTATCGAG
CAGCCATTGTCGGGACAAGCCTATTTGTCATTGTCAATATTATCTGGTCATTTATTAACTTCCGAAAATATTTACGGAAA
CGGAGTATTATGGAATGA

Upstream 100 bases:

>100_bases
AAAAATATGCAGATATAATTTTAGATAATTCTGGAACTATTGATACACTAAAGAAACAAATCCAGCAAGAATTGGCTAGG
ATAATCAAAGAGAAATAGGG

Downstream 100 bases:

>100_bases
GAGTTAAAATTACATTAATTTGTAGTTCTTGTGGAAATAAAAACTATATTAGTAGCAAAAATAAGGCCACACATCCAGAA
AAAGTGGAAACTATGAAATT

Product: multidrug resistance efflux pump

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNHTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPALASGLVAPIWGRLADEHGRK
VMMVRASIVMTLTMGGIAFVPNVWWLLGLRLLMGFFSGYIPNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGL
FAEWFGMANVFLIVGALLALATLLTIFFVHENFEPIAKGEMLSSREIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
YIKTLTTSTNNLMFISGLIVSAVGLSAMMSSSFLGRLGDKYGSHRLILIGLVFTFVIYLPMAFVQSPLQLGILRFLLGFG
AGALMPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGPLVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRK
RSIME

Sequences:

>Translated_405_residues
MNHTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPALASGLVAPIWGRLADEHGRK
VMMVRASIVMTLTMGGIAFVPNVWWLLGLRLLMGFFSGYIPNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGL
FAEWFGMANVFLIVGALLALATLLTIFFVHENFEPIAKGEMLSSREIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
YIKTLTTSTNNLMFISGLIVSAVGLSAMMSSSFLGRLGDKYGSHRLILIGLVFTFVIYLPMAFVQSPLQLGILRFLLGFG
AGALMPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGPLVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRK
RSIME
>Mature_405_residues
MNHTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPALASGLVAPIWGRLADEHGRK
VMMVRASIVMTLTMGGIAFVPNVWWLLGLRLLMGFFSGYIPNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGL
FAEWFGMANVFLIVGALLALATLLTIFFVHENFEPIAKGEMLSSREIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
YIKTLTTSTNNLMFISGLIVSAVGLSAMMSSSFLGRLGDKYGSHRLILIGLVFTFVIYLPMAFVQSPLQLGILRFLLGFG
AGALMPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGPLVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRK
RSIME

Specific function: Efflux pump for various substrates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=405, Percent_Identity=43.4567901234568, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI87082404, Length=392, Percent_Identity=25, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43935; Mature: 43935

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPA
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHH
LASGLVAPIWGRLADEHGRKVMMVRASIVMTLTMGGIAFVPNVWWLLGLRLLMGFFSGYI
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
PNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGLFAEWFGMANVFLIVGALLAL
CCCCEEHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATLLTIFFVHENFEPIAKGEMLSSREIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
HHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
YIKTLTTSTNNLMFISGLIVSAVGLSAMMSSSFLGRLGDKYGSHRLILIGLVFTFVIYLP
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
MAFVQSPLQLGILRFLLGFGAGALMPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGP
HHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
LVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRKRSIME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNHTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPA
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHH
LASGLVAPIWGRLADEHGRKVMMVRASIVMTLTMGGIAFVPNVWWLLGLRLLMGFFSGYI
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
PNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGLFAEWFGMANVFLIVGALLAL
CCCCEEHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATLLTIFFVHENFEPIAKGEMLSSREIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
HHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
YIKTLTTSTNNLMFISGLIVSAVGLSAMMSSSFLGRLGDKYGSHRLILIGLVFTFVIYLP
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
MAFVQSPLQLGILRFLLGFGAGALMPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGP
HHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
LVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRKRSIME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]