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The map label for this gene is greA [H]

Identifier: 125623471

GI number: 125623471

Start: 599021

End: 599491

Strand: Direct

Name: greA [H]

Synonym: llmg_0610

Alternate gene names: 125623471

Gene position: 599021-599491 (Clockwise)

Preceding gene: 125623470

Following gene: 125623472

Centisome position: 23.68

GC content: 35.46

Gene sequence:

>471_bases
ATGGAAAAAACATTCCCAATGACCAAAGAAGGTCTTGATAAATTAAAAGCAGAACTTGAAAATTTAAAACTCGTTAAACG
TCCAGAAGTTATTGACCGTATCAAAGTTGCGCGTAGCTATGGTGACCTTTCAGAAAACTCAGAATATGAAGCAGCTAAAG
ATGAACAAGCCTTTATTGAAGGACGTATTTCAACTGTTGAAACAATGATTCGTTACGCAGAAATTGTTGACAATGCTAAA
ATTGCTAAAGATGAAGTGGCACTTGGTAAAAATGTTACTTTTGTGGAAGTCGGAGAAACTGACGAAGAATCATATCAAAT
CGTTGGTACTGCTGAAGCAGACCCATTTACAGGTAAAATTTCAAATGAATCACCAATTGCTCGTGTTTTGATTGGTAAAA
AAGTTGGAGATATCGTTGATGTCCCATTGCCAGTTGGCGAAATGACAGTAAAAATTGTTAAAGTTGATTAA

Upstream 100 bases:

>100_bases
AAATGTTCAAAAATATGTGAATGATAAATTAAGTAAATAGAGTATGATTGATTTTGATTTAACTAAAAAGAAAAGTTTAT
AATAAAAGGAAAGAAATAAA

Downstream 100 bases:

>100_bases
TTGAATGAGAACTTAATAATAAGAAAGCCGTGAGTTGTAGTGACCCCCAAAATATAGACTTTTCAAATCTATATTTCGGA
GGTCTTTTCTTATGGTCAAA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MEKTFPMTKEGLDKLKAELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIEGRISTVETMIRYAEIVDNAK
IAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFTGKISNESPIARVLIGKKVGDIVDVPLPVGEMTVKIVKVD

Sequences:

>Translated_156_residues
MEKTFPMTKEGLDKLKAELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIEGRISTVETMIRYAEIVDNAK
IAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFTGKISNESPIARVLIGKKVGDIVDVPLPVGEMTVKIVKVD
>Mature_156_residues
MEKTFPMTKEGLDKLKAELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIEGRISTVETMIRYAEIVDNAK
IAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFTGKISNESPIARVLIGKKVGDIVDVPLPVGEMTVKIVKVD

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=155, Percent_Identity=43.2258064516129, Blast_Score=123, Evalue=5e-30,
Organism=Escherichia coli, GI87082261, Length=152, Percent_Identity=34.8684210526316, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17247; Mature: 17247

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTFPMTKEGLDKLKAELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
GRISTVETMIRYAEIVDNAKIAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFTGKI
HHHHHHHHHHHHHHHHCCCHHCHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCC
SNESPIARVLIGKKVGDIVDVPLPVGEMTVKIVKVD
CCCCCHHHHHHCCCCCCEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure
MEKTFPMTKEGLDKLKAELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
GRISTVETMIRYAEIVDNAKIAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFTGKI
HHHHHHHHHHHHHHHHCCCHHCHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCC
SNESPIARVLIGKKVGDIVDVPLPVGEMTVKIVKVD
CCCCCHHHHHHCCCCCCEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]