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The map label for this gene is ogt [H]

Identifier: 125623365

GI number: 125623365

Start: 491341

End: 491850

Strand: Direct

Name: ogt [H]

Synonym: llmg_0498

Alternate gene names: 125623365

Gene position: 491341-491850 (Clockwise)

Preceding gene: 125623363

Following gene: 125623366

Centisome position: 19.42

GC content: 34.12

Gene sequence:

>510_bases
ATGTTATATAAAGCAAGCTATCAATCCCCCTTGGGAGAAATGAGTCTTCTGAGTGATGAGACCTATTTGCTAGGACTATG
GTTTAATGAACAAAAATATTTTGGTTCCGCATATGATTTAAACTTAGCCCAAAAAGATGAAAGTTTACCAATTACTGAAG
CAATTGAATGGTTAAATCAGTATTTTAGAGGAGAAAATCCGAATTCTCAAAAAATCAAATTAAAACCGGAAGTAACAGAA
TTTAGAAAAAGGGTTCTTAAAATACTCAGTGATATTCCTTATGGGACTTTAATCACTTATAAAGAAATTTCAAATCTTCT
TCAAATCAATGATGAAAAGAAACGAAATATGTCGCAAGCTGTTGGGGGAGCAGTCGGTCATAATCCAATATCTATCATTA
TTCCTTGTCATCGAGTAGTGGGCAGTGATGGAAGTTTAACAGGTTATGCGGGTGGCTTAGATAAAAAAAGGGCGCTCTTG
GCTCTCGAACAAAATAGTTTAATCAAATAA

Upstream 100 bases:

>100_bases
TAAAGAAGAATAATGAAAAAGCCAAATAGTGATGTTTTATAGTCGATTTTTTTGTTATACTAATGATTATAAAAAATAAG
GACTAAATAGGAGAGGACGA

Downstream 100 bases:

>100_bases
ATAGGAGCTTTCATTTGAAAATAACTGATGAAATCTGGGAAGTTATTAAAGATAACGATAAAAAACTTGATAATAAACTG
TGGTATGGAGTGGCAACAAC

Product: 6-O-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MLYKASYQSPLGEMSLLSDETYLLGLWFNEQKYFGSAYDLNLAQKDESLPITEAIEWLNQYFRGENPNSQKIKLKPEVTE
FRKRVLKILSDIPYGTLITYKEISNLLQINDEKKRNMSQAVGGAVGHNPISIIIPCHRVVGSDGSLTGYAGGLDKKRALL
ALEQNSLIK

Sequences:

>Translated_169_residues
MLYKASYQSPLGEMSLLSDETYLLGLWFNEQKYFGSAYDLNLAQKDESLPITEAIEWLNQYFRGENPNSQKIKLKPEVTE
FRKRVLKILSDIPYGTLITYKEISNLLQINDEKKRNMSQAVGGAVGHNPISIIIPCHRVVGSDGSLTGYAGGLDKKRALL
ALEQNSLIK
>Mature_169_residues
MLYKASYQSPLGEMSLLSDETYLLGLWFNEQKYFGSAYDLNLAQKDESLPITEAIEWLNQYFRGENPNSQKIKLKPEVTE
FRKRVLKILSDIPYGTLITYKEISNLLQINDEKKRNMSQAVGGAVGHNPISIIIPCHRVVGSDGSLTGYAGGLDKKRALL
ALEQNSLIK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=184, Percent_Identity=31.5217391304348, Blast_Score=74, Evalue=8e-14,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=38.6792452830189, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788542, Length=85, Percent_Identity=38.8235294117647, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19027; Mature: 19027

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYKASYQSPLGEMSLLSDETYLLGLWFNEQKYFGSAYDLNLAQKDESLPITEAIEWLNQ
CCCCCCCCCCCCCHHHCCCCEEEEEEEECCHHHCCCEEECCCCCCCCCCCHHHHHHHHHH
YFRGENPNSQKIKLKPEVTEFRKRVLKILSDIPYGTLITYKEISNLLQINDEKKRNMSQA
HHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCHHHHHHHHH
VGGAVGHNPISIIIPCHRVVGSDGSLTGYAGGLDKKRALLALEQNSLIK
HCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLYKASYQSPLGEMSLLSDETYLLGLWFNEQKYFGSAYDLNLAQKDESLPITEAIEWLNQ
CCCCCCCCCCCCCHHHCCCCEEEEEEEECCHHHCCCEEECCCCCCCCCCCHHHHHHHHHH
YFRGENPNSQKIKLKPEVTEFRKRVLKILSDIPYGTLITYKEISNLLQINDEKKRNMSQA
HHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCHHHHHHHHH
VGGAVGHNPISIIIPCHRVVGSDGSLTGYAGGLDKKRALLALEQNSLIK
HCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]