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The map label for this gene is mdh [C]

Identifier: 125623342

GI number: 125623342

Start: 467926

End: 468840

Strand: Direct

Name: mdh [C]

Synonym: llmg_0475

Alternate gene names: 125623342

Gene position: 467926-468840 (Clockwise)

Preceding gene: 125623341

Following gene: 125623348

Centisome position: 18.5

GC content: 36.5

Gene sequence:

>915_bases
ATGCGAAAAGTAGGATTAATTGGTTACGGTCATGTTGGGGCAACAGTTGCTTTGGATATCGTTCAGGGGGGCTTCGCTGA
CGAGCTAGTAATTATTGACAAGGATAGAAAAAAAGCAGAGGCAGAGGTTTTAGACCTCCTAGATGCCCTTGCTTTGCTTC
CATTCTATGTCAAAATATACGTGGGAGAATATGATGATTTAGCGACTGCCGATATTGTCTTATCTACATTGGGTCATATC
GAACTAATTAAACCGGGCGGAGATAGATTCACTGAATTAAAAGCAAATATTCCAGAAATTAAAGAAGTTTCTGAGCAATT
AAACAGGATTAATTTCAAAGGAATTTTGATTGCAACAACCAATCCGAACGATGTGATTGTTAATATTTATTCTCAATTAT
TAAATCTTCCTAAATCACAGATTATTGGAACGGGGACTTGTCTGGACACGGCACGTATGAAAGCTCAAGTAAGTCGAGCG
TTGGAAATTGATGCCCGAAGTGTTGAAGGTTATGTTTTGGGGGAACATGGTAATTCACAATTTACGGCTTGGTCAACGGT
CAGAGTTGGTGGTCAATCTTTTTTAGAGATTGCTAAAGAAAAAAATTTAAATTTAAAAGACTTAGAAGAAAAAGCCCGTC
AAGGAGGATTTGCTGTATTTAACACAAAAGGATATACCAACGTAGCCATAGCCGCAGCAACGGTTTCCTTGATGAATCTG
GTGCTTTCTGATGCAAAAAATATTGCTATCTGCTCACATTATGACGAAAATTTTAGTTCATATATTTCGACACCTGCCTT
GATTGGTAAAAATGGGATAGAAGCATTGATAAAACTTCCACTAACTTTAGAAGAAGAAGGCAAATTAAAAAATTCAGTGG
TTGAAATTCAGGAAAAAATAAATTATTTTAGTTAA

Upstream 100 bases:

>100_bases
TCTTCTTGCACCGAACTTGGTTAGAAAATGGAACTTTTGAAGAATTTGCTACTTTCTTAAAAGAATATTATGATAAAAAG
TAGCTGAAAAAGGAGAAAAA

Downstream 100 bases:

>100_bases
AAGTAAAAAGCCTGTTCAATCGAACAGACTTTTTTTAATCAGCATTAGCAATGCCTTCTTCTTCCTCATCATCAGTATTT
TCTTCAATACTATCAAGAGT

Product: malate/lactate dehydrogenase

Products: H+; Nicotinamide adenine dinucleotide - reduced; Oxaloacetate [C]

Alternate protein names: L-HicDH [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRKVGLIGYGHVGATVALDIVQGGFADELVIIDKDRKKAEAEVLDLLDALALLPFYVKIYVGEYDDLATADIVLSTLGHI
ELIKPGGDRFTELKANIPEIKEVSEQLNRINFKGILIATTNPNDVIVNIYSQLLNLPKSQIIGTGTCLDTARMKAQVSRA
LEIDARSVEGYVLGEHGNSQFTAWSTVRVGGQSFLEIAKEKNLNLKDLEEKARQGGFAVFNTKGYTNVAIAAATVSLMNL
VLSDAKNIAICSHYDENFSSYISTPALIGKNGIEALIKLPLTLEEEGKLKNSVVEIQEKINYFS

Sequences:

>Translated_304_residues
MRKVGLIGYGHVGATVALDIVQGGFADELVIIDKDRKKAEAEVLDLLDALALLPFYVKIYVGEYDDLATADIVLSTLGHI
ELIKPGGDRFTELKANIPEIKEVSEQLNRINFKGILIATTNPNDVIVNIYSQLLNLPKSQIIGTGTCLDTARMKAQVSRA
LEIDARSVEGYVLGEHGNSQFTAWSTVRVGGQSFLEIAKEKNLNLKDLEEKARQGGFAVFNTKGYTNVAIAAATVSLMNL
VLSDAKNIAICSHYDENFSSYISTPALIGKNGIEALIKLPLTLEEEGKLKNSVVEIQEKINYFS
>Mature_304_residues
MRKVGLIGYGHVGATVALDIVQGGFADELVIIDKDRKKAEAEVLDLLDALALLPFYVKIYVGEYDDLATADIVLSTLGHI
ELIKPGGDRFTELKANIPEIKEVSEQLNRINFKGILIATTNPNDVIVNIYSQLLNLPKSQIIGTGTCLDTARMKAQVSRA
LEIDARSVEGYVLGEHGNSQFTAWSTVRVGGQSFLEIAKEKNLNLKDLEEKARQGGFAVFNTKGYTNVAIAAATVSLMNL
VLSDAKNIAICSHYDENFSSYISTPALIGKNGIEALIKLPLTLEEEGKLKNSVVEIQEKINYFS

Specific function: Catalyzes the NADP dependent reversible and stereospecific interconversion between 2-ketocarboxylic acids and L-2-hydroxy-carboxylic acids. 2-ketoacids with medium chain length (five to six C-atoms) are the best substrates [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=311, Percent_Identity=29.2604501607717, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI221136809, Length=311, Percent_Identity=29.2604501607717, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI15082234, Length=311, Percent_Identity=30.2250803858521, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI5031857, Length=302, Percent_Identity=28.8079470198675, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI260099723, Length=302, Percent_Identity=28.8079470198675, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI291575128, Length=300, Percent_Identity=28.6666666666667, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4557032, Length=300, Percent_Identity=28.6666666666667, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI9257228, Length=301, Percent_Identity=26.578073089701, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4504973, Length=301, Percent_Identity=26.578073089701, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI207028494, Length=180, Percent_Identity=30.5555555555556, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI260099725, Length=223, Percent_Identity=27.3542600896861, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260099727, Length=193, Percent_Identity=29.5336787564767, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI103472011, Length=317, Percent_Identity=21.4511041009464, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=299, Percent_Identity=28.0936454849498, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550422, Length=301, Percent_Identity=28.9036544850498, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17136226, Length=301, Percent_Identity=26.578073089701, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: 1.1.1.37 [C]

Molecular weight: Translated: 33176; Mature: 33176

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVGLIGYGHVGATVALDIVQGGFADELVIIDKDRKKAEAEVLDLLDALALLPFYVKIY
CCCEEEEEECCCCHHHEEEHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEE
VGEYDDLATADIVLSTLGHIELIKPGGDRFTELKANIPEIKEVSEQLNRINFKGILIATT
ECCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEC
NPNDVIVNIYSQLLNLPKSQIIGTGTCLDTARMKAQVSRALEIDARSVEGYVLGEHGNSQ
CCCHHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
FTAWSTVRVGGQSFLEIAKEKNLNLKDLEEKARQGGFAVFNTKGYTNVAIAAATVSLMNL
EEEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHH
VLSDAKNIAICSHYDENFSSYISTPALIGKNGIEALIKLPLTLEEEGKLKNSVVEIQEKI
HHHCCCCEEEEECCCHHHHHHHCCCHHHCCCHHHHHHCCCCEECCCCCHHHHHHHHHHHH
NYFS
CCCC
>Mature Secondary Structure
MRKVGLIGYGHVGATVALDIVQGGFADELVIIDKDRKKAEAEVLDLLDALALLPFYVKIY
CCCEEEEEECCCCHHHEEEHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEE
VGEYDDLATADIVLSTLGHIELIKPGGDRFTELKANIPEIKEVSEQLNRINFKGILIATT
ECCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEC
NPNDVIVNIYSQLLNLPKSQIIGTGTCLDTARMKAQVSRALEIDARSVEGYVLGEHGNSQ
CCCHHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
FTAWSTVRVGGQSFLEIAKEKNLNLKDLEEKARQGGFAVFNTKGYTNVAIAAATVSLMNL
EEEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHH
VLSDAKNIAICSHYDENFSSYISTPALIGKNGIEALIKLPLTLEEEGKLKNSVVEIQEKI
HHHCCCCEEEEECCCHHHHHHHCCCHHHCCCHHHHHHCCCCEECCCCCHHHHHHHHHHHH
NYFS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Malate; Nicotinamide adenine dinucleotide [C]

Specific reaction: L-Malate + Nicotinamide adenine dinucleotide <==> H+ + Nicotinamide adenine dinucleotide - reduced + Oxaloacetate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684788; 7643402 [H]