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The map label for this gene is noxE

Identifier: 125623275

GI number: 125623275

Start: 401822

End: 403162

Strand: Direct

Name: noxE

Synonym: llmg_0408

Alternate gene names: 125623275

Gene position: 401822-403162 (Clockwise)

Preceding gene: 125623274

Following gene: 125623276

Centisome position: 15.89

GC content: 36.39

Gene sequence:

>1341_bases
ATGAAAATCGTAGTTATCGGTACAAACCACGCAGGCATTGCTACAGCGAATACATTACTTGAACAATATCCCGGGCATGA
AATTGTCATGATTGACCGTAATAGCAACATGAGTTATCTAGGTTGTGGCACAGCAATTTGGGTTGGAAGACAAATTGAAA
AACCAGATGAATTATTTTATGCCAAAGCAGAGGATTTTGAGGCAAAAGGGGTAAAAATTTTGACTGAAACAGAAGTTTCA
GAAATTGATTTTGCTAATAAGAAAGTTTATGCAAAAACTAAATCTGATGATGAAATAATTGAAGCTTACGACAAGCTTGT
TTTAGCAACAGGTTCACGTCCAATTATTCCTAATCTACCAGGCAAAGACCTTAAGGGAATTCATTTTCTGAAACTTTTTC
AAGAAGGTCAAGCAATTGACGCAGAATTTGCCAAAGAAAAAGTCAAGCGTATCGCAGTCATTGGTGCAGGATATATCGGT
ACAGAGATTGCGGAAGCAGCTAAACGTCGGGGTAAAGAAGTTCTTCTCTTTGACGCTGAAAATACTTCACTTGCATCATA
TTATGATGAAGAATTTGCCAAAGGAATGGATGAAAACCTTGCTCAACATGGAATTGAACTTCATTTTGGAGAACTGGCCA
AAGAATTTAAAGCGAATGAGGAAGGTTATGTATCACAAATCGTAACCAACAAGGCGACTTATGATGTTGATCTTGTCATC
AATTGTATTGGTTTTACTGCCAACAGTGCCTTGGCAAGTGATAAGTTAGCTACCTTCAAAAATGGCGCAATCAAGGTGGA
TAAGCATCAACAAAGTAGTGATCCAGATGTTTACGCGGTAGGTGATGTTGCGACAATTTATTCTAATGCCTTGCAAGATT
TTACTTATATCGCTCTTGCCTCAAACGCTGTTCGGTCAGGAATTGTCGCAGGACACAATATTGGTGGAAAAGAATTAGAA
TCTGTTGGTGTTCAAGGTTCTAATGGTATTTCGATTTTTGGTTACAATATGACTTCTACAGGACTTTCTGTTAAAGCTGC
TAAAAAATTAGGTTTAGAAGTTTCATTTAGTGATTTTGAAGATAAACAAAAAGCTTGGTTTCTTCATGAAAACAACGATA
GTGTGAAAATTCGTATCGTATATGAGACAAAAAGTCGCAGAATTATTGGAGCACAACTTGCTAGTAAAAGTGAGATAATT
GCAGGAAATATAAATATGTTCAGTTTAGCGATTCAAGAGAAAAAAACAATTGATGAACTAGCTTTGCTTGATTTATTCTT
TCTCCCCCACTTCAACAGTCCATATAATTATATGACAGTTGCAGCTTTGAATGCCAAATAA

Upstream 100 bases:

>100_bases
ATTTCACAATGTTCACAAGCGCTTACAAAAGAAAATAGATTGACTTATGCTAAACTGAATAATGTAAAAAGAATTTTACA
TTTAAAGGAGACCTATTAGT

Downstream 100 bases:

>100_bases
ACAATAGAAATCTATCTGCTTGATAGATTTTTTTATTTTTTAGTGACAAATGCTCAAACTTTACGTATAGAAAGTTAGGA
GGATTTTATAATGAATAAAA

Product: NADH oxidase

Products: NA

Alternate protein names: NOXase

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKIVVIGTNHAGIATANTLLEQYPGHEIVMIDRNSNMSYLGCGTAIWVGRQIEKPDELFYAKAEDFEAKGVKILTETEVS
EIDFANKKVYAKTKSDDEIIEAYDKLVLATGSRPIIPNLPGKDLKGIHFLKLFQEGQAIDAEFAKEKVKRIAVIGAGYIG
TEIAEAAKRRGKEVLLFDAENTSLASYYDEEFAKGMDENLAQHGIELHFGELAKEFKANEEGYVSQIVTNKATYDVDLVI
NCIGFTANSALASDKLATFKNGAIKVDKHQQSSDPDVYAVGDVATIYSNALQDFTYIALASNAVRSGIVAGHNIGGKELE
SVGVQGSNGISIFGYNMTSTGLSVKAAKKLGLEVSFSDFEDKQKAWFLHENNDSVKIRIVYETKSRRIIGAQLASKSEII
AGNINMFSLAIQEKKTIDELALLDLFFLPHFNSPYNYMTVAALNAK

Sequences:

>Translated_446_residues
MKIVVIGTNHAGIATANTLLEQYPGHEIVMIDRNSNMSYLGCGTAIWVGRQIEKPDELFYAKAEDFEAKGVKILTETEVS
EIDFANKKVYAKTKSDDEIIEAYDKLVLATGSRPIIPNLPGKDLKGIHFLKLFQEGQAIDAEFAKEKVKRIAVIGAGYIG
TEIAEAAKRRGKEVLLFDAENTSLASYYDEEFAKGMDENLAQHGIELHFGELAKEFKANEEGYVSQIVTNKATYDVDLVI
NCIGFTANSALASDKLATFKNGAIKVDKHQQSSDPDVYAVGDVATIYSNALQDFTYIALASNAVRSGIVAGHNIGGKELE
SVGVQGSNGISIFGYNMTSTGLSVKAAKKLGLEVSFSDFEDKQKAWFLHENNDSVKIRIVYETKSRRIIGAQLASKSEII
AGNINMFSLAIQEKKTIDELALLDLFFLPHFNSPYNYMTVAALNAK
>Mature_446_residues
MKIVVIGTNHAGIATANTLLEQYPGHEIVMIDRNSNMSYLGCGTAIWVGRQIEKPDELFYAKAEDFEAKGVKILTETEVS
EIDFANKKVYAKTKSDDEIIEAYDKLVLATGSRPIIPNLPGKDLKGIHFLKLFQEGQAIDAEFAKEKVKRIAVIGAGYIG
TEIAEAAKRRGKEVLLFDAENTSLASYYDEEFAKGMDENLAQHGIELHFGELAKEFKANEEGYVSQIVTNKATYDVDLVI
NCIGFTANSALASDKLATFKNGAIKVDKHQQSSDPDVYAVGDVATIYSNALQDFTYIALASNAVRSGIVAGHNIGGKELE
SVGVQGSNGISIFGYNMTSTGLSVKAAKKLGLEVSFSDFEDKQKAWFLHENNDSVKIRIVYETKSRRIIGAQLASKSEII
AGNINMFSLAIQEKKTIDELALLDLFFLPHFNSPYNYMTVAALNAK

Specific function: Catalyzes the four-electron reduction of molecular oxygen to water. Active on beta-NADH, but not on alpha-NADH, beta- NADPH or alpha-NADPH. Under aerobic conditions, oxygen acts as the electron acceptor. Under anaerobic conditions, DCIP and MB can replace

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI65787454, Length=284, Percent_Identity=23.2394366197183, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI21389617, Length=284, Percent_Identity=23.2394366197183, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI226437568, Length=284, Percent_Identity=23.2394366197183, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1789065, Length=252, Percent_Identity=28.968253968254, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789915, Length=215, Percent_Identity=30.2325581395349, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082354, Length=355, Percent_Identity=26.1971830985915, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=222, Percent_Identity=26.5765765765766, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24639257, Length=324, Percent_Identity=23.7654320987654, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281359715, Length=324, Percent_Identity=23.7654320987654, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281359713, Length=324, Percent_Identity=23.7654320987654, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639250, Length=324, Percent_Identity=23.7654320987654, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI18543267, Length=324, Percent_Identity=23.7654320987654, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639252, Length=324, Percent_Identity=23.7654320987654, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAOX_LACLM (A2RIB7)

Other databases:

- EMBL:   AY046926
- EMBL:   AM406671
- RefSeq:   YP_001031758.1
- ProteinModelPortal:   A2RIB7
- SMR:   A2RIB7
- STRING:   A2RIB7
- GeneID:   4796799
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_0408
- eggNOG:   COG0446
- HOGENOM:   HBG535576
- OMA:   DICEDEM
- ProtClustDB:   CLSK697203
- GO:   GO:0005737
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PRINTS:   PR00368

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.6.99.3

Molecular weight: Translated: 48872; Mature: 48872

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 42-42 BINDING 42-42 BINDING 159-159 BINDING 178-178 BINDING 187-187 BINDING 244-244 BINDING 282-282 BINDING 298-298 BINDING 300-300 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVIGTNHAGIATANTLLEQYPGHEIVMIDRNSNMSYLGCGTAIWVGRQIEKPDELFY
CEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCEEEEECCCCCCCHHEEE
AKAEDFEAKGVKILTETEVSEIDFANKKVYAKTKSDDEIIEAYDKLVLATGSRPIIPNLP
EECCCCCCCCEEEEEECCHHHCCCCCCEEEEECCCCHHHHHHHHHEEEECCCCCCCCCCC
GKDLKGIHFLKLFQEGQAIDAEFAKEKVKRIAVIGAGYIGTEIAEAAKRRGKEVLLFDAE
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHHCCCEEEEEECC
NTSLASYYDEEFAKGMDENLAQHGIELHFGELAKEFKANEEGYVSQIVTNKATYDVDLVI
CCCHHHHHHHHHHCCHHHHHHHCCCEEEHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEE
NCIGFTANSALASDKLATFKNGAIKVDKHQQSSDPDVYAVGDVATIYSNALQDFTYIALA
EEECCCCCCHHCCCCHHHCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHEEEEEH
SNAVRSGIVAGHNIGGKELESVGVQGSNGISIFGYNMTSTGLSVKAAKKLGLEVSFSDFE
HHHHHHCCEECCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEHHHCCCEEEECCCC
DKQKAWFLHENNDSVKIRIVYETKSRRIIGAQLASKSEIIAGNINMFSLAIQEKKTIDEL
CCCEEEEEEECCCEEEEEEEEECCCCEEEEHHHCCCCCEEECCCEEEEEEEHHHHHHHHH
ALLDLFFLPHFNSPYNYMTVAALNAK
HHHHHHHCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKIVVIGTNHAGIATANTLLEQYPGHEIVMIDRNSNMSYLGCGTAIWVGRQIEKPDELFY
CEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCEEEEECCCCCCCHHEEE
AKAEDFEAKGVKILTETEVSEIDFANKKVYAKTKSDDEIIEAYDKLVLATGSRPIIPNLP
EECCCCCCCCEEEEEECCHHHCCCCCCEEEEECCCCHHHHHHHHHEEEECCCCCCCCCCC
GKDLKGIHFLKLFQEGQAIDAEFAKEKVKRIAVIGAGYIGTEIAEAAKRRGKEVLLFDAE
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHHCCCEEEEEECC
NTSLASYYDEEFAKGMDENLAQHGIELHFGELAKEFKANEEGYVSQIVTNKATYDVDLVI
CCCHHHHHHHHHHCCHHHHHHHCCCEEEHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEE
NCIGFTANSALASDKLATFKNGAIKVDKHQQSSDPDVYAVGDVATIYSNALQDFTYIALA
EEECCCCCCHHCCCCHHHCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHEEEEEH
SNAVRSGIVAGHNIGGKELESVGVQGSNGISIFGYNMTSTGLSVKAAKKLGLEVSFSDFE
HHHHHHCCEECCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEHHHCCCEEEECCCC
DKQKAWFLHENNDSVKIRIVYETKSRRIIGAQLASKSEIIAGNINMFSLAIQEKKTIDEL
CCCEEEEEEECCCEEEEEEEEECCCCEEEEHHHCCCCCEEECCCEEEEEEEHHHHHHHHH
ALLDLFFLPHFNSPYNYMTVAALNAK
HHHHHHHCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932446