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The map label for this gene is tyrS [H]

Identifier: 125623269

GI number: 125623269

Start: 395229

End: 396488

Strand: Reverse

Name: tyrS [H]

Synonym: llmg_0401

Alternate gene names: 125623269

Gene position: 396488-395229 (Counterclockwise)

Preceding gene: 125623271

Following gene: 125623268

Centisome position: 15.67

GC content: 37.46

Gene sequence:

>1260_bases
ATGAACATTTTTGACGAACTTAAAGCACGTGGTTTGGTTTTCCAAACAACGGACGAAGCTGCGCTTTCTAAAGCGCTGAC
GGAGGATATGGTTTCCTATTATGTGGGCTATGATCCTACTGCTGATTCCCTTCATTTAGGTAATCTTGTACTCATTTTGA
CAATGAAACGTCTGCAAATGGCTGGTCACAAACCCTATGCACTTGTTGGTGGTGCAACTGGTTTGATTGGTGACCCTTCT
TTTAAAGATTCAGAACGTTCGCTCCAAACAAAAGACACCGTAACAAAATGGTCTGGAAAAATCCGTAGTCAACTTGAGCG
TTTCCTAGATTTTGAAAATGGTGAAAACAAGGCAGAAATGACCAATAACTACAACTGGTTTGAAAACCTGACATTTATTG
ATTTTCTCAGAGATGTAGGTAAACATTTTACTGTAAATTACATGATTTCTAAAGACTCTGTAAAATCACGCATGGAAAGC
GGAATATCTTATACAGAATTTGCTTATCAAATTATGCAAGGTTATGATTTCTACGAATTGAACCAATTGCATAATGTCAC
TTTACAACTTGGTGGTTCTGACCAATGGGGTAATATGACTGCTGGTACAGAGCTTTTACGTCGTAAGGCTAACAAACAAG
GACATGTTATCACTATTCCTTTAATTACAGATTCAACTGGTAAAAAATTCGGTAAATCAGAGGGAAATGCAATTTGGCTT
GATGCTGATAAAACCTCTCCTTATGAAATGTACCAGTTTTGGCTTAATGTTGATGATGCAGACGCTGTGAAAATGCTGAA
AATTTTCACCTTCCTCTCATTAGAAGAGATTGCTGAAATTGAAGAACAATTTGAGGCTGCTCGTCATGAACGTTTGGCTC
AAAAAGTTTTGGCTCGTGAAGTTGTAAGTCTCGTTCATGGAAAAGAAGCTTATGAACAAGCTGTTAAAACTTCTGAAATC
TTGTTTGGTGGTGGAGATTTACGTCAATTGGATGCTAAATCAATCCTAACAGGCTTGAAAGCAGCGCCACAACATCAAAT
CGCTCCTGATGAAGACCTGACTCTAATTGAGCTTTTAATTTCTGTAGGAATTGCTCCTTCAAAGAGACAAGCTCGTGAAG
ATATTACAAATGGAGCAATTTATATCAACGGAGAAAGAGTACAAGAACTTGATTATGTTCTTACAGATTCCGATAAAATT
GAGAATCGTCTGACAGTCATTCGTCGTGGAAAAAAGAAAAACTTTGTTTTGACTTATTAA

Upstream 100 bases:

>100_bases
AATTTTTTTTAGTAATTTGTTATAATGTAAAATGGTGCTGTTATTTTTTTACTTTTTAATCCAAAGGGAACAGCTCAATT
ATTATAGAAAGGATTTTTTT

Downstream 100 bases:

>100_bases
TTATCAAAAATTCCTGCTATAAAATGAGCAGGAATTTTATTTTAGAAAAATTTAAGCGTAGCTCTTTCATTTAATCTACA
ATATGTTATAATAACTGTAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNIFDELKARGLVFQTTDEAALSKALTEDMVSYYVGYDPTADSLHLGNLVLILTMKRLQMAGHKPYALVGGATGLIGDPS
FKDSERSLQTKDTVTKWSGKIRSQLERFLDFENGENKAEMTNNYNWFENLTFIDFLRDVGKHFTVNYMISKDSVKSRMES
GISYTEFAYQIMQGYDFYELNQLHNVTLQLGGSDQWGNMTAGTELLRRKANKQGHVITIPLITDSTGKKFGKSEGNAIWL
DADKTSPYEMYQFWLNVDDADAVKMLKIFTFLSLEEIAEIEEQFEAARHERLAQKVLAREVVSLVHGKEAYEQAVKTSEI
LFGGGDLRQLDAKSILTGLKAAPQHQIAPDEDLTLIELLISVGIAPSKRQAREDITNGAIYINGERVQELDYVLTDSDKI
ENRLTVIRRGKKKNFVLTY

Sequences:

>Translated_419_residues
MNIFDELKARGLVFQTTDEAALSKALTEDMVSYYVGYDPTADSLHLGNLVLILTMKRLQMAGHKPYALVGGATGLIGDPS
FKDSERSLQTKDTVTKWSGKIRSQLERFLDFENGENKAEMTNNYNWFENLTFIDFLRDVGKHFTVNYMISKDSVKSRMES
GISYTEFAYQIMQGYDFYELNQLHNVTLQLGGSDQWGNMTAGTELLRRKANKQGHVITIPLITDSTGKKFGKSEGNAIWL
DADKTSPYEMYQFWLNVDDADAVKMLKIFTFLSLEEIAEIEEQFEAARHERLAQKVLAREVVSLVHGKEAYEQAVKTSEI
LFGGGDLRQLDAKSILTGLKAAPQHQIAPDEDLTLIELLISVGIAPSKRQAREDITNGAIYINGERVQELDYVLTDSDKI
ENRLTVIRRGKKKNFVLTY
>Mature_419_residues
MNIFDELKARGLVFQTTDEAALSKALTEDMVSYYVGYDPTADSLHLGNLVLILTMKRLQMAGHKPYALVGGATGLIGDPS
FKDSERSLQTKDTVTKWSGKIRSQLERFLDFENGENKAEMTNNYNWFENLTFIDFLRDVGKHFTVNYMISKDSVKSRMES
GISYTEFAYQIMQGYDFYELNQLHNVTLQLGGSDQWGNMTAGTELLRRKANKQGHVITIPLITDSTGKKFGKSEGNAIWL
DADKTSPYEMYQFWLNVDDADAVKMLKIFTFLSLEEIAEIEEQFEAARHERLAQKVLAREVVSLVHGKEAYEQAVKTSEI
LFGGGDLRQLDAKSILTGLKAAPQHQIAPDEDLTLIELLISVGIAPSKRQAREDITNGAIYINGERVQELDYVLTDSDKI
ENRLTVIRRGKKKNFVLTY

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=402, Percent_Identity=36.5671641791045, Blast_Score=242, Evalue=5e-64,
Organism=Escherichia coli, GI1787925, Length=429, Percent_Identity=48.7179487179487, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145802, Length=310, Percent_Identity=39.0322580645161, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI25145805, Length=246, Percent_Identity=36.5853658536585, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6325160, Length=445, Percent_Identity=37.752808988764, Blast_Score=290, Evalue=2e-79,
Organism=Drosophila melanogaster, GI20130383, Length=437, Percent_Identity=38.9016018306636, Blast_Score=305, Evalue=3e-83,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47375; Mature: 47375

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFDELKARGLVFQTTDEAALSKALTEDMVSYYVGYDPTADSLHLGNLVLILTMKRLQM
CCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHH
AGHKPYALVGGATGLIGDPSFKDSERSLQTKDTVTKWSGKIRSQLERFLDFENGENKAEM
CCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
TNNYNWFENLTFIDFLRDVGKHFTVNYMISKDSVKSRMESGISYTEFAYQIMQGYDFYEL
CCCCCHHHCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
NQLHNVTLQLGGSDQWGNMTAGTELLRRKANKQGHVITIPLITDSTGKKFGKSEGNAIWL
HHHCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEE
DADKTSPYEMYQFWLNVDDADAVKMLKIFTFLSLEEIAEIEEQFEAARHERLAQKVLARE
ECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVSLVHGKEAYEQAVKTSEILFGGGDLRQLDAKSILTGLKAAPQHQIAPDEDLTLIELLI
HHHHHHCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
SVGIAPSKRQAREDITNGAIYINGERVQELDYVLTDSDKIENRLTVIRRGKKKNFVLTY
HHCCCCCHHHHHHHHCCCEEEECCCHHHHHHHEECCCHHHHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MNIFDELKARGLVFQTTDEAALSKALTEDMVSYYVGYDPTADSLHLGNLVLILTMKRLQM
CCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHH
AGHKPYALVGGATGLIGDPSFKDSERSLQTKDTVTKWSGKIRSQLERFLDFENGENKAEM
CCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
TNNYNWFENLTFIDFLRDVGKHFTVNYMISKDSVKSRMESGISYTEFAYQIMQGYDFYEL
CCCCCHHHCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
NQLHNVTLQLGGSDQWGNMTAGTELLRRKANKQGHVITIPLITDSTGKKFGKSEGNAIWL
HHHCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEE
DADKTSPYEMYQFWLNVDDADAVKMLKIFTFLSLEEIAEIEEQFEAARHERLAQKVLARE
ECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVSLVHGKEAYEQAVKTSEILFGGGDLRQLDAKSILTGLKAAPQHQIAPDEDLTLIELLI
HHHHHHCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
SVGIAPSKRQAREDITNGAIYINGERVQELDYVLTDSDKIENRLTVIRRGKKKNFVLTY
HHCCCCCHHHHHHHHCCCEEEECCCHHHHHHHEECCCHHHHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]