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The map label for this gene is rnjA [H]

Identifier: 125623174

GI number: 125623174

Start: 291217

End: 292902

Strand: Reverse

Name: rnjA [H]

Synonym: llmg_0302

Alternate gene names: 125623174

Gene position: 292902-291217 (Counterclockwise)

Preceding gene: 125623175

Following gene: 125623173

Centisome position: 11.58

GC content: 42.17

Gene sequence:

>1686_bases
ATGCCAAAACAACTTAAAATAAAAGCTGATGAAGTCGCAGTTTACGCCATTGGCGGACTGGGCGAAATCGGCAAAAACAC
TTACGGCGTTGAATATAAAGATGAAATCATCATCATCGACGCCGGAATTAAATTCCCAGAAGACGATTTATTAGGTGTTG
ACTACGTCATCCCTGATTACACTTATATTGTTGAAAATATCGACCGGATTAAAGGACTCTTCATTACTCACGGACACGAA
GACCATATCGGAGGAATTCCTTTCCTTTTGAAACAAGCCAATATCCCTATTTATGCTGGGCCATTGTCACTTGCACTCAT
CCGTGGAAAGCTCGAAGAACACGGGCTTTTACGTGATGCTAAACTCTACGAAATCCACGCCGATTCAGTCATTAAATTTA
ATCACTTGAGTCTTGATTTCTTCCGCACCACTCACTCAATTCCAGAACCTTTAGGAATTGTTGTGAATACCCCTCAAGGT
AAAATTGTAGCGACCGGTGACTTTAAGTTTGACTTCACACCAGTGGGTGAACCTGCTGATCTACACCGTATGGCTGAACT
TGGTAGCGAAGGTGTCTTACTCCTTCTTTCTGATTCAACCAATGCTGAAATTCCAACTTTTTCACATTCTGAAAAAGTCG
TTGGTGCAAGCATCATGAAAATTTTTGAACGTATTGAAGGTCGGATTATTTTCGCTTCTTTTGCCTCAAATATTTTCCGT
TTGCAACAAGCCGTTGATGGAGCAGTTAAGACTGGGCGTAAAATTGTTGTCTTTGGGCGTTCAATGGAAAAAGCCATTGT
TAACGGTTTAGAACTTGGTTATATCAAAGCGCCTAAGGGCACATTTATCGAACCAAATGAAGTCAATCAATATGCTTCAC
ATGAACTTTTGATTATGTGTACAGGTTCGCAAGGTGAGCCAATGGCTGCACTATCAAGAATTGCCAACGGAATGCACCGT
CATATTACCCTACAAATGGGCGATACCGTTGTCTTCTCATCAAATGCGATTCCTGGAAATACTCATGGTGTTAACCAACT
GATTAACAAAATCGCTGAAGCTGGAGTTGAAGTCATTTATGGTAAGATGAACAACATTCACACTTCAGGTCACGGTGGAC
AACAAGAACAAAAACTCATGTTGCGTCTTATCAAACCAAAATACTTTATGCCTGTCCACGGTGAGTATCGTATGCTCAAA
ATTCATGCTGGACTTGCTCAAGATACTGGTGTCCCTAAAGAAAATTGTTTCATTCAAGAAAACGGAGATGTCCTTGCACT
GACTGCTGACAGCGCCAGATATGCTGACCATTTCCCGGCTGCCGATACTTACGTTGACGGTTCAGGAATTGGAGATGTCG
GAAATGCCGTTCTCCATGACCGTCATGAACTTTCAGAAGACGGAATCGTTCTCGCAGTGGCAACCGTTGACTTTGAAAAG
AAAACCGTTCTTGCTGGACCTGATATGCTCTCACGTGGATTCATTTACATGCGTGAGTCTGGTGATTTGATTCGTAGTGG
CCAACGTGTCCTTTTCAATGCTATACGTGAAGCCATGCATGAAGAAAATGCTAATGAAGCGTCTGTAGCACGTGCGATGC
GTGAAGCACTCTCACCATTCCTTTATAAACAAACTGAGCGCTCACCACTTGTTGTTCCAATGATTATGACACCTGATGAA
AAATAA

Upstream 100 bases:

>100_bases
CAGTCTGTATTCAGTCCTGTCACCAAATTTTTAACTCTTGATACTTAACTGACCCATTGTCAGTAAAAATAACTAATAAA
ATCCAAAGAATAGGAGAAAC

Downstream 100 bases:

>100_bases
AAAATTACTGACAGAACCAAATTCTTATTAGAATGGAAGTCTGTCAGTATTTTTTATTCAAAATAATCTTTTTTCTTGAC
TTAAACCTGGTTTAAGCTGA

Product: putative Zn-dependent hydrolase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MPKQLKIKADEVAVYAIGGLGEIGKNTYGVEYKDEIIIIDAGIKFPEDDLLGVDYVIPDYTYIVENIDRIKGLFITHGHE
DHIGGIPFLLKQANIPIYAGPLSLALIRGKLEEHGLLRDAKLYEIHADSVIKFNHLSLDFFRTTHSIPEPLGIVVNTPQG
KIVATGDFKFDFTPVGEPADLHRMAELGSEGVLLLLSDSTNAEIPTFSHSEKVVGASIMKIFERIEGRIIFASFASNIFR
LQQAVDGAVKTGRKIVVFGRSMEKAIVNGLELGYIKAPKGTFIEPNEVNQYASHELLIMCTGSQGEPMAALSRIANGMHR
HITLQMGDTVVFSSNAIPGNTHGVNQLINKIAEAGVEVIYGKMNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMLK
IHAGLAQDTGVPKENCFIQENGDVLALTADSARYADHFPAADTYVDGSGIGDVGNAVLHDRHELSEDGIVLAVATVDFEK
KTVLAGPDMLSRGFIYMRESGDLIRSGQRVLFNAIREAMHEENANEASVARAMREALSPFLYKQTERSPLVVPMIMTPDE
K

Sequences:

>Translated_561_residues
MPKQLKIKADEVAVYAIGGLGEIGKNTYGVEYKDEIIIIDAGIKFPEDDLLGVDYVIPDYTYIVENIDRIKGLFITHGHE
DHIGGIPFLLKQANIPIYAGPLSLALIRGKLEEHGLLRDAKLYEIHADSVIKFNHLSLDFFRTTHSIPEPLGIVVNTPQG
KIVATGDFKFDFTPVGEPADLHRMAELGSEGVLLLLSDSTNAEIPTFSHSEKVVGASIMKIFERIEGRIIFASFASNIFR
LQQAVDGAVKTGRKIVVFGRSMEKAIVNGLELGYIKAPKGTFIEPNEVNQYASHELLIMCTGSQGEPMAALSRIANGMHR
HITLQMGDTVVFSSNAIPGNTHGVNQLINKIAEAGVEVIYGKMNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMLK
IHAGLAQDTGVPKENCFIQENGDVLALTADSARYADHFPAADTYVDGSGIGDVGNAVLHDRHELSEDGIVLAVATVDFEK
KTVLAGPDMLSRGFIYMRESGDLIRSGQRVLFNAIREAMHEENANEASVARAMREALSPFLYKQTERSPLVVPMIMTPDE
K
>Mature_560_residues
PKQLKIKADEVAVYAIGGLGEIGKNTYGVEYKDEIIIIDAGIKFPEDDLLGVDYVIPDYTYIVENIDRIKGLFITHGHED
HIGGIPFLLKQANIPIYAGPLSLALIRGKLEEHGLLRDAKLYEIHADSVIKFNHLSLDFFRTTHSIPEPLGIVVNTPQGK
IVATGDFKFDFTPVGEPADLHRMAELGSEGVLLLLSDSTNAEIPTFSHSEKVVGASIMKIFERIEGRIIFASFASNIFRL
QQAVDGAVKTGRKIVVFGRSMEKAIVNGLELGYIKAPKGTFIEPNEVNQYASHELLIMCTGSQGEPMAALSRIANGMHRH
ITLQMGDTVVFSSNAIPGNTHGVNQLINKIAEAGVEVIYGKMNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMLKI
HAGLAQDTGVPKENCFIQENGDVLALTADSARYADHFPAADTYVDGSGIGDVGNAVLHDRHELSEDGIVLAVATVDFEKK
TVLAGPDMLSRGFIYMRESGDLIRSGQRVLFNAIREAMHEENANEASVARAMREALSPFLYKQTERSPLVVPMIMTPDEK

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61629; Mature: 61497

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQLKIKADEVAVYAIGGLGEIGKNTYGVEYKDEIIIIDAGIKFPEDDLLGVDYVIPDY
CCCCEEEEECCEEEEEECCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCEEECCCH
TYIVENIDRIKGLFITHGHEDHIGGIPFLLKQANIPIYAGPLSLALIRGKLEEHGLLRDA
HHHHHHHHHHCEEEEECCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHCCCCCCC
KLYEIHADSVIKFNHLSLDFFRTTHSIPEPLGIVVNTPQGKIVATGDFKFDFTPVGEPAD
EEEEEECCCEEEEECEEEEEEHHHCCCCCCCEEEEECCCCCEEEECCEEEEECCCCCCHH
LHRMAELGSEGVLLLLSDSTNAEIPTFSHSEKVVGASIMKIFERIEGRIIFASFASNIFR
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
LQQAVDGAVKTGRKIVVFGRSMEKAIVNGLELGYIKAPKGTFIEPNEVNQYASHELLIMC
HHHHHHHHHHCCCEEEEECCCHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEE
TGSQGEPMAALSRIANGMHRHITLQMGDTVVFSSNAIPGNTHGVNQLINKIAEAGVEVIY
ECCCCCHHHHHHHHHCCCEEEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEE
GKMNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMLKIHAGLAQDTGVPKENCFIQE
ECCCCEEECCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEECCCHHCCCCCCCCEEEEC
NGDVLALTADSARYADHFPAADTYVDGSGIGDVGNAVLHDRHELSEDGIVLAVATVDFEK
CCCEEEEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHCHHHCCCCCEEEEEEEECCCC
KTVLAGPDMLSRGFIYMRESGDLIRSGQRVLFNAIREAMHEENANEASVARAMREALSPF
CEEEECCCHHHCCEEEEECCCCHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHH
LYKQTERSPLVVPMIMTPDEK
HHHCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
PKQLKIKADEVAVYAIGGLGEIGKNTYGVEYKDEIIIIDAGIKFPEDDLLGVDYVIPDY
CCCEEEEECCEEEEEECCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCEEECCCH
TYIVENIDRIKGLFITHGHEDHIGGIPFLLKQANIPIYAGPLSLALIRGKLEEHGLLRDA
HHHHHHHHHHCEEEEECCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHCCCCCCC
KLYEIHADSVIKFNHLSLDFFRTTHSIPEPLGIVVNTPQGKIVATGDFKFDFTPVGEPAD
EEEEEECCCEEEEECEEEEEEHHHCCCCCCCEEEEECCCCCEEEECCEEEEECCCCCCHH
LHRMAELGSEGVLLLLSDSTNAEIPTFSHSEKVVGASIMKIFERIEGRIIFASFASNIFR
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
LQQAVDGAVKTGRKIVVFGRSMEKAIVNGLELGYIKAPKGTFIEPNEVNQYASHELLIMC
HHHHHHHHHHCCCEEEEECCCHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEE
TGSQGEPMAALSRIANGMHRHITLQMGDTVVFSSNAIPGNTHGVNQLINKIAEAGVEVIY
ECCCCCHHHHHHHHHCCCEEEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEE
GKMNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMLKIHAGLAQDTGVPKENCFIQE
ECCCCEEECCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEECCCHHCCCCCCCCEEEEC
NGDVLALTADSARYADHFPAADTYVDGSGIGDVGNAVLHDRHELSEDGIVLAVATVDFEK
CCCEEEEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHCHHHCCCCCEEEEEEEECCCC
KTVLAGPDMLSRGFIYMRESGDLIRSGQRVLFNAIREAMHEENANEASVARAMREALSPF
CEEEECCCHHHCCEEEEECCCCHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHH
LYKQTERSPLVVPMIMTPDEK
HHHCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]