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The map label for this gene is rlmN [H]

Identifier: 125623071

GI number: 125623071

Start: 182618

End: 183715

Strand: Direct

Name: rlmN [H]

Synonym: llmg_0194

Alternate gene names: 125623071

Gene position: 182618-183715 (Clockwise)

Preceding gene: 125623070

Following gene: 125623072

Centisome position: 7.22

GC content: 36.7

Gene sequence:

>1098_bases
ATGACAGAGAATATAACAACAGAAACACGTCCGTCTATCTATGGATTGACACGTGATCAATTAATCGAATGGGCCATTGA
AAATGGTGAAAAGAAATTCCGAGCAACACAAGTTTGGGATTGGCTTTACCGCAAACGTGTTCAATCTTTTGAAGAAATGA
GCAACTTATCAGCGGTATTTATTGATAAACTTAATGAAGCTTTCATTTTGAATCCATTGGAACAAGTGGTTGTGCAAGAG
TCTGCTGATGGAACAGTTAAATACCTCTTTATGCTTCCTGATAAAGTGATGATTGAAACAGTTTTAATGCGCCAATCTTA
TGGTTTGTCTGTCTGTGTCACCACTCAAGTGGGCTGCAATATGGGCTGTACCTTCTGTGCTTCTGGAATTTTGAAAAAAG
AACGCGATGTAACAGCCGGTGAAATCGTTAGTCAAATCATGCTTGTACAAAAGTATTTTGATGAACGTGGTTTAGATGAA
CGTGTTTCGCATGTTGTTGTGATGGGAATTGGTGAACCGTTTGATAATTATGAACATTTGATGAACTTTTTACGTGTGAT
TAATGATGATAATGGACTTGCTATCGGTGCTCGTCATATTACTGTTTCTACTTGCGGATTTATGCCAGCAAAAATCAAAG
AATTTGCTCATGACAATCTTCAAATCAATCTAGCTATTTCACTCCATGCTCCAAATAATGAATTGCGTACTTCATTGATG
CGAATTACTCGTAATGCCCCTCTTGAAAAACTCTTTGAGGCGATTGATTATTATACAGAAACGACCAATCGACGGGTGAC
TTATGAGTACATTATGTTGTCTGGTGAAAATGACAGCCCTGAAATCGCGCAACAGCTGGCAGATTTAATTAAATCCCGTA
ATAAATTGTCTTATGTCAACTTGATTCCTTATAATCCAGTTGCTGAACATATCAAGTATGAACGTTCAACAAAAGATAAT
ACTGCAAAATTCTATGATGTTTTGAAGAAAAATGGTATTAACTGTGTCGTTCGTCAAGAACATGGAACAGATATTGATGC
GGCTTGTGGACAATTAAGATCAAAACAAATTAAGAAAAATAAGGCCAAATTAGCCTAA

Upstream 100 bases:

>100_bases
AATTGAAAATAATTAGACGAATATAAAGTAGTTTTGATTGTGTATTCAAGGGATATGAAATCGTTCACAATTAGAATTCT
ACATAACGATAAGGAAAATA

Downstream 100 bases:

>100_bases
GAAAAAACTGCTGACCAAGCAGTTTTTTTTGCTGACAGTTCTGTCAGTAAAATATTTCGGAAATAGCTTAATTTTTAATA
AAAAATAAGAACAATGAATA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTENITTETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAVFIDKLNEAFILNPLEQVVVQE
SADGTVKYLFMLPDKVMIETVLMRQSYGLSVCVTTQVGCNMGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDE
RVSHVVVMGIGEPFDNYEHLMNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHDNLQINLAISLHAPNNELRTSLM
RITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKSRNKLSYVNLIPYNPVAEHIKYERSTKDN
TAKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKNKAKLA

Sequences:

>Translated_365_residues
MTENITTETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAVFIDKLNEAFILNPLEQVVVQE
SADGTVKYLFMLPDKVMIETVLMRQSYGLSVCVTTQVGCNMGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDE
RVSHVVVMGIGEPFDNYEHLMNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHDNLQINLAISLHAPNNELRTSLM
RITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKSRNKLSYVNLIPYNPVAEHIKYERSTKDN
TAKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKNKAKLA
>Mature_364_residues
TENITTETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAVFIDKLNEAFILNPLEQVVVQES
ADGTVKYLFMLPDKVMIETVLMRQSYGLSVCVTTQVGCNMGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDER
VSHVVVMGIGEPFDNYEHLMNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHDNLQINLAISLHAPNNELRTSLMR
ITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKSRNKLSYVNLIPYNPVAEHIKYERSTKDNT
AKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKNKAKLA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=368, Percent_Identity=35.8695652173913, Blast_Score=221, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41659; Mature: 41528

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENITTETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAVF
CCCCCCCCCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IDKLNEAFILNPLEQVVVQESADGTVKYLFMLPDKVMIETVLMRQSYGLSVCVTTQVGCN
HHHCCCCEECCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCC
MGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDERVSHVVVMGIGEPFDNYEHL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCEEEEEECCCCCCCHHHH
MNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHDNLQINLAISLHAPNNELRTSLM
HHHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHH
RITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKSRNKLSYVN
HHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
LIPYNPVAEHIKYERSTKDNTAKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKN
ECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KAKLA
HCCCC
>Mature Secondary Structure 
TENITTETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAVF
CCCCCCCCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IDKLNEAFILNPLEQVVVQESADGTVKYLFMLPDKVMIETVLMRQSYGLSVCVTTQVGCN
HHHCCCCEECCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCC
MGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDERVSHVVVMGIGEPFDNYEHL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCEEEEEECCCCCCCHHHH
MNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHDNLQINLAISLHAPNNELRTSLM
HHHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHH
RITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKSRNKLSYVN
HHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
LIPYNPVAEHIKYERSTKDNTAKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKN
ECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KAKLA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]