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The map label for this gene is blt [H]

Identifier: 125623012

GI number: 125623012

Start: 127087

End: 128250

Strand: Direct

Name: blt [H]

Synonym: llmg_0133

Alternate gene names: 125623012

Gene position: 127087-128250 (Clockwise)

Preceding gene: 125623011

Following gene: 125623013

Centisome position: 5.02

GC content: 36.08

Gene sequence:

>1164_bases
ATGAAAAACAAAAAAAGCATGATGAATTTAGCAATTTCTAATCTGTTTTTAGTATTTTTGGGTGCAGGTTTAGTCATTCC
TGTTTTGCCAACCCTTAAAGAACAAATGCACTTTTCTGGGACAACAATGGGAATGATGATTTCTATTTTTGCCATTGCTC
AGTTAATTGCCTCACCGATTGCTGGTGCTTTATCTGATAAAATTGGGCGTAAAAAACTCATTGCGATCGGAATGATTATT
TTTTCTTTTTCCGAACTTTTATTTGGACTGGCGCAAGCGAAAACAGGCTTTTATATTTCACGAGCTTTAGGAGGCGTTGC
AGCGGCAATGCTGATGCCATCAGTTACAGCTTATGTGGCTGACATGACTACAATTTCCGAACGCCCTAAGGCAATGGGAC
TCGTATCAGCAGCAATTAGTGGTGGGTTTATCATTGGACCCGGTGTAGGTGGTTTCATCGCTCATTTTGGTATCCGTGTT
CCTTTCTATGTTGCGGCAATTTTAGCTTTTATTGGTTTTATTTTAACAATCACGATTTTAAAAGAACCTGAACGGACAAT
GGAAGCCAATCATGAAACCGAAAAAGTTTCATTCCTTGATATTCTCAAGAACCCATTATTTGGCTCATTATTTATCATTA
TTTTGATATCATCATTTGGATTACAAGCTTTTGAGTCAATTTATAGTATCATGGCAAGCATTAACTTTGGATTTAGCATG
GGGGAAATTGCTTTAGTCATTACAGTAAGTGGTATCCTGGCCCTCTTTTTCCAACTCTTTTTATTTGATACAATTGTCAA
TAAAATCGGAGAACTTGGTCTAATTCAACTGACTTTCTTTGCAAGTGCAATTTTTATAGCAATTATTGCATTTACGAAAA
CAAATTTAGTTGTGGCTCTTTCGACCTTTGTTGTATTCTTAGCTTTTGACCTGTTCAGACCAGCTGTGACGACCTATTTA
TCGAAACATGCTGGAAATCGTCAGGGAGCAATTAATGGTTTAAACTCGGCCTTTACAAGCTTTGGTAATATCTTAGGGCC
AATGGCTGCAGGATATATGTTTGATATTAATCATCTATTCCCTTACTATATCTCTGCGATTATTTTACTTGGAACAGGAT
TTTTATCATTATTCTTAAATAGAAAAAATTTGAGTAAAGCTTAA

Upstream 100 bases:

>100_bases
TTTAGTATATAGATTTCATGATATAATAAGAGAATTGGTAAAAAATAAAAGAAATAGCAGTAGCTGAGTCTATTTGCTAT
TTTTGAATGAGGAGGAAAAA

Downstream 100 bases:

>100_bases
ATCACTTTTCAGATAGAAAGTCTATGGTCATTCGTAGGCTTTTTTATTTATATTCAAGACTTCTAGAGAAATTATAAAAC
ATTAAAAAACTTCATTACAT

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLIASPIAGALSDKIGRKKLIAIGMII
FSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVADMTTISERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRV
PFYVAAILAFIGFILTITILKEPERTMEANHETEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
GEIALVITVSGILALFFQLFLFDTIVNKIGELGLIQLTFFASAIFIAIIAFTKTNLVVALSTFVVFLAFDLFRPAVTTYL
SKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDINHLFPYYISAIILLGTGFLSLFLNRKNLSKA

Sequences:

>Translated_387_residues
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLIASPIAGALSDKIGRKKLIAIGMII
FSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVADMTTISERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRV
PFYVAAILAFIGFILTITILKEPERTMEANHETEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
GEIALVITVSGILALFFQLFLFDTIVNKIGELGLIQLTFFASAIFIAIIAFTKTNLVVALSTFVVFLAFDLFRPAVTTYL
SKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDINHLFPYYISAIILLGTGFLSLFLNRKNLSKA
>Mature_387_residues
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLIASPIAGALSDKIGRKKLIAIGMII
FSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVADMTTISERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRV
PFYVAAILAFIGFILTITILKEPERTMEANHETEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
GEIALVITVSGILALFFQLFLFDTIVNKIGELGLIQLTFFASAIFIAIIAFTKTNLVVALSTFVVFLAFDLFRPAVTTYL
SKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDINHLFPYYISAIILLGTGFLSLFLNRKNLSKA

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=363, Percent_Identity=26.4462809917355, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787291, Length=362, Percent_Identity=29.0055248618785, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081738, Length=370, Percent_Identity=27.8378378378378, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17137160, Length=228, Percent_Identity=25.8771929824561, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI221513297, Length=361, Percent_Identity=26.5927977839335, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41644; Mature: 41644

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLIASPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AGALSDKIGRKKLIAIGMIIFSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DMTTISERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRVPFYVAAILAFIGFILTITIL
HHHHHHHCCHHHHHHHHHHCCCEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
KEPERTMEANHETEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
CCCHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCH
GEIALVITVSGILALFFQLFLFDTIVNKIGELGLIQLTFFASAIFIAIIAFTKTNLVVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STFVVFLAFDLFRPAVTTYLSKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDINHLF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PYYISAIILLGTGFLSLFLNRKNLSKA
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLIASPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AGALSDKIGRKKLIAIGMIIFSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DMTTISERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRVPFYVAAILAFIGFILTITIL
HHHHHHHCCHHHHHHHHHHCCCEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
KEPERTMEANHETEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
CCCHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCH
GEIALVITVSGILALFFQLFLFDTIVNKIGELGLIQLTFFASAIFIAIIAFTKTNLVVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STFVVFLAFDLFRPAVTTYLSKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDINHLF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PYYISAIILLGTGFLSLFLNRKNLSKA
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]