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The map label for this gene is mesJ [H]

Identifier: 125622900

GI number: 125622900

Start: 21334

End: 22602

Strand: Direct

Name: mesJ [H]

Synonym: llmg_0019

Alternate gene names: 125622900

Gene position: 21334-22602 (Clockwise)

Preceding gene: 125622899

Following gene: 125622901

Centisome position: 0.84

GC content: 34.44

Gene sequence:

>1269_bases
ATGACACCAGCTCAACTTAAATTTCTAAAAATAGTAACTAAAGAAAAATACTTTGCGGAAAATTCGAAAATTTTACTGGC
TTTATCAGGTGGTAAAGACTCAATGACCCTCTTTAATTGGCTCTATGAGCTTAGAGCAAAGCTCCATATAGAATTGGGAA
TAGCTCACATTAATCATGGCTTACGACCCGAATCAGACTTTGAAGAACAAGCCTTACGTAAAATGGCCAGTGACCTAGAG
ATTCCAATTTTTGTTGACAGATTTACAGGAGAGTTTACAGAGCAAAAAGCTCGGGAGTTTCGCTACCACTTTTTTGAAAA
AATAATGATAGCTGAAAATTATGATGTTCTTCTGACTGCACACCATCAAGGCGATGTCGTTGAAACTGTATTAATGCGTC
AGATTACTGGGCGTCCTTTGAGAAGTTTACAGGGAATTGCTGACTGTCAACCCTTTGCTAAGGGACAATTAATTCGTCCA
TTACTCAAATTTACAAAAGAAGAGCTTGATGCGGCGGTCTTTTATGAAGATTCGACCAATCAAGGAGTAGATTATTTTAG
AAATAGAATACGGAATCAGCTTATTCCCGAATTAACAAAAGAAAATCCTCAATTTTCGCAGGCAATCAGTGATTTAAGCT
CAGAAATAAAAATGGCCTTAACAGTGATTAATCAAAAAATTTCGGAACTTGAAATTGTTGATGAAAAAATCTCTTATCAC
AAATTTATTTCTCAAACAAAAGAATTACAGCACTTCATTTTACAAGCATTTTTTGCTCAGTACCCAGAAATAAAAGTAAG
TAAGAAAAAATTTGAAGAAATCTTACATATTATCAGACGTCCACAACAATATTCTGCTCAATTGAATAAAGATTTTCAGT
TTGTGAAGACCAAAGATTACTTTTATATTGAAAAAAGGCAGTTAATCACAGCTTCACTCGAAATTTTAAGTGAAAATCCT
AACGATGCAAGTTTTATGGAAGTATACTTACCTTTAGAAGGGCAATTAGAAATTCGCAAGCGACAACCTGGTGATCAAAT
ATTGATTAATGGTCACCATAAAAAATTACGAAAGTTTTTTATTGACAATCATGTTCCCTTACAAGCTCGTGAGAACCCTC
TCATCTTTGTCGAAAAAAAACTTTACGCAATCGTCGGAGTCGCATGCTCTGATTTGAGTAAAGAGCTCAAAAATGATAAA
ATTAGAAGAATATTATGGGTCAAACCCAGTATAGGAGAGGAAATCAACGATGCTCGAAAAAAATCTTGA

Upstream 100 bases:

>100_bases
TTATCTTTACAAGTGAGCCCTACCTTTTGGTCATTGAAACAAATGGTGGAAGTGATGATGAAATAAAGAATATTTCACAA
GATGTTTATGGAGTTATGAA

Downstream 100 bases:

>100_bases
TAAGGCCATTGAAAAAGCACTAGTTTCAGAAGAAGAAATTATTGAAAAATCCAAAGAACTTGGAGAAATATTAACAAAAG
AATACGAAGGGAAAAATCCT

Product: cell cycle protein mesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTPAQLKFLKIVTKEKYFAENSKILLALSGGKDSMTLFNWLYELRAKLHIELGIAHINHGLRPESDFEEQALRKMASDLE
IPIFVDRFTGEFTEQKAREFRYHFFEKIMIAENYDVLLTAHHQGDVVETVLMRQITGRPLRSLQGIADCQPFAKGQLIRP
LLKFTKEELDAAVFYEDSTNQGVDYFRNRIRNQLIPELTKENPQFSQAISDLSSEIKMALTVINQKISELEIVDEKISYH
KFISQTKELQHFILQAFFAQYPEIKVSKKKFEEILHIIRRPQQYSAQLNKDFQFVKTKDYFYIEKRQLITASLEILSENP
NDASFMEVYLPLEGQLEIRKRQPGDQILINGHHKKLRKFFIDNHVPLQARENPLIFVEKKLYAIVGVACSDLSKELKNDK
IRRILWVKPSIGEEINDARKKS

Sequences:

>Translated_422_residues
MTPAQLKFLKIVTKEKYFAENSKILLALSGGKDSMTLFNWLYELRAKLHIELGIAHINHGLRPESDFEEQALRKMASDLE
IPIFVDRFTGEFTEQKAREFRYHFFEKIMIAENYDVLLTAHHQGDVVETVLMRQITGRPLRSLQGIADCQPFAKGQLIRP
LLKFTKEELDAAVFYEDSTNQGVDYFRNRIRNQLIPELTKENPQFSQAISDLSSEIKMALTVINQKISELEIVDEKISYH
KFISQTKELQHFILQAFFAQYPEIKVSKKKFEEILHIIRRPQQYSAQLNKDFQFVKTKDYFYIEKRQLITASLEILSENP
NDASFMEVYLPLEGQLEIRKRQPGDQILINGHHKKLRKFFIDNHVPLQARENPLIFVEKKLYAIVGVACSDLSKELKNDK
IRRILWVKPSIGEEINDARKKS
>Mature_421_residues
TPAQLKFLKIVTKEKYFAENSKILLALSGGKDSMTLFNWLYELRAKLHIELGIAHINHGLRPESDFEEQALRKMASDLEI
PIFVDRFTGEFTEQKAREFRYHFFEKIMIAENYDVLLTAHHQGDVVETVLMRQITGRPLRSLQGIADCQPFAKGQLIRPL
LKFTKEELDAAVFYEDSTNQGVDYFRNRIRNQLIPELTKENPQFSQAISDLSSEIKMALTVINQKISELEIVDEKISYHK
FISQTKELQHFILQAFFAQYPEIKVSKKKFEEILHIIRRPQQYSAQLNKDFQFVKTKDYFYIEKRQLITASLEILSENPN
DASFMEVYLPLEGQLEIRKRQPGDQILINGHHKKLRKFFIDNHVPLQARENPLIFVEKKLYAIVGVACSDLSKELKNDKI
RRILWVKPSIGEEINDARKKS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=210, Percent_Identity=30.4761904761905, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 49323; Mature: 49191

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAQLKFLKIVTKEKYFAENSKILLALSGGKDSMTLFNWLYELRAKLHIELGIAHINHG
CCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEHHHHCCC
LRPESDFEEQALRKMASDLEIPIFVDRFTGEFTEQKAREFRYHFFEKIMIAENYDVLLTA
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
HHQGDVVETVLMRQITGRPLRSLQGIADCQPFAKGQLIRPLLKFTKEELDAAVFYEDSTN
CCCCHHHHHHHHHHHCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCC
QGVDYFRNRIRNQLIPELTKENPQFSQAISDLSSEIKMALTVINQKISELEIVDEKISYH
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFISQTKELQHFILQAFFAQYPEIKVSKKKFEEILHIIRRPQQYSAQLNKDFQFVKTKDY
HHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCE
FYIEKRQLITASLEILSENPNDASFMEVYLPLEGQLEIRKRQPGDQILINGHHKKLRKFF
EEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCCEEEECCHHHHHHHHH
IDNHVPLQARENPLIFVEKKLYAIVGVACSDLSKELKNDKIRRILWVKPSIGEEINDARK
HCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHC
KS
CC
>Mature Secondary Structure 
TPAQLKFLKIVTKEKYFAENSKILLALSGGKDSMTLFNWLYELRAKLHIELGIAHINHG
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEHHHHCCC
LRPESDFEEQALRKMASDLEIPIFVDRFTGEFTEQKAREFRYHFFEKIMIAENYDVLLTA
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
HHQGDVVETVLMRQITGRPLRSLQGIADCQPFAKGQLIRPLLKFTKEELDAAVFYEDSTN
CCCCHHHHHHHHHHHCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCC
QGVDYFRNRIRNQLIPELTKENPQFSQAISDLSSEIKMALTVINQKISELEIVDEKISYH
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFISQTKELQHFILQAFFAQYPEIKVSKKKFEEILHIIRRPQQYSAQLNKDFQFVKTKDY
HHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCE
FYIEKRQLITASLEILSENPNDASFMEVYLPLEGQLEIRKRQPGDQILINGHHKKLRKFF
EEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCCCEEEECCHHHHHHHHH
IDNHVPLQARENPLIFVEKKLYAIVGVACSDLSKELKNDKIRRILWVKPSIGEEINDARK
HCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]