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The map label for this gene is thiC

Identifier: 124486523

GI number: 124486523

Start: 1747266

End: 1748543

Strand: Reverse

Name: thiC

Synonym: Mlab_1711

Alternate gene names: 124486523

Gene position: 1748543-1747266 (Counterclockwise)

Preceding gene: 124486524

Following gene: 124486521

Centisome position: 96.87

GC content: 55.56

Gene sequence:

>1278_bases
ATGACAATCGTTGAAGATGCAAAAAAAGGCCTCATCACTGAAGAGATGAAGGTCGTGGCAAAATCCGAAGGGGTCACCGA
AGATTTCATCCGCAGGGGAATTGCCGGCGGTCATATCGTCATCCCGATGACGCCCTACCGGAAAGTAAAACTCTGCGGTA
TCGGCTCGGGACTTCGCACAAAAGTGAATGCATCGATTGGGACCTCGTCTGATATCGTGAATGTGGAAGAGGAGCTGGAA
AAGGCACGTCAGGCAGAACTCGCCGGGGCAGACTCGCTGATGGAGCTTTCGACAGGCGGGGACTTCCTCGATATCCGCCG
CCGGGTCTGTGAACAGTCGAACCTTTCCGTTGGATCCGTTCCGCTCTATCAGGCGTTCATCGAAGCTGCCAGAAACAAAG
GCGGCGTCGTTTTCATGGACGAGGACGATCTCTTCAAGATCACCGAGCAGCAGGCAAAACTCGGTACGAATTTTATGGCG
ATCCACACCGGAATCAACTACGAGACCGTGAAGAGACTGAAAAATCAGGGCAGACACGGCGGTCTCGTCTCCCGTGGAGG
GGCATTCATGACGGCATGGATGCTGCACAACGAGATGGAGAACCCTCTCTACCGCAGGTTCGACTACCTCGTCGAGATCT
TAAAGGAACACGAAGTCACCCTCTCCTTTGGAAACGGCATGCGGGCAGGGGCCTGTCATGACGCCACGGATCGTGCCGCC
ATCCAGGAACTTTTGATCAACGCAGAACTCGCCGATCAGGCACACAACGCCGGTGTTCAGTGCATTCTCGAAGGTCCGGG
TCACATCCCTCTCGATGAGATCAAAACCAATGTCCAGCTGGAAAAACGGGTCACCAACAACAAACCGTTCTATATGCTCG
GTCCTCTGGTAACGGATATCGCTCCGGGATACGACGACCGCGTTGCTGCGATCGGAGCTTCCGTCTCGTCCGCCGCAGGT
GCAGATTTCATCTGCTATGTAACCCCGGCCGAGCATCTGGCTCTTCCGACCCCCGAAGAGGTCTATGAGGGAGTTATGAG
TTCACGCATCGCGGCCCACGTCGGCGATATGGTCAAACTCCCAAAGACCCGTGAAGCCGATCTCGAGATGGGGCACGCCC
GCCGCGACCTCGACTGGGAACGCCAGTATGCCGTCTCGATAAACGCAGAGAAGGCACGCTGCATCAGAAACTCCCGCATG
CCGGCCGATTCCGATGCCTGCACGATGTGCGGCGATTTCTGTGCGATCAAGATCGTCCAGAAGACGTTTAATTTCTGA

Upstream 100 bases:

>100_bases
CCTATAAAATGACATTTTACTTGTTAAATCAGCAAATAAATTTGTTTTTATTTATAGTAAATAAAATAGTTTATAATCTC
CAGCATCCTGAAGTAAGGGT

Downstream 100 bases:

>100_bases
AGCGGGTTCATCCGCAGGATGGACCTTAGCGGAGAAAACCGGAGGTTTTCGTTAGGCATGTGAATATGCATGCCCTTACG
GGTTCATCCGCAGACCATCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTIVEDAKKGLITEEMKVVAKSEGVTEDFIRRGIAGGHIVIPMTPYRKVKLCGIGSGLRTKVNASIGTSSDIVNVEEELE
KARQAELAGADSLMELSTGGDFLDIRRRVCEQSNLSVGSVPLYQAFIEAARNKGGVVFMDEDDLFKITEQQAKLGTNFMA
IHTGINYETVKRLKNQGRHGGLVSRGGAFMTAWMLHNEMENPLYRRFDYLVEILKEHEVTLSFGNGMRAGACHDATDRAA
IQELLINAELADQAHNAGVQCILEGPGHIPLDEIKTNVQLEKRVTNNKPFYMLGPLVTDIAPGYDDRVAAIGASVSSAAG
ADFICYVTPAEHLALPTPEEVYEGVMSSRIAAHVGDMVKLPKTREADLEMGHARRDLDWERQYAVSINAEKARCIRNSRM
PADSDACTMCGDFCAIKIVQKTFNF

Sequences:

>Translated_425_residues
MTIVEDAKKGLITEEMKVVAKSEGVTEDFIRRGIAGGHIVIPMTPYRKVKLCGIGSGLRTKVNASIGTSSDIVNVEEELE
KARQAELAGADSLMELSTGGDFLDIRRRVCEQSNLSVGSVPLYQAFIEAARNKGGVVFMDEDDLFKITEQQAKLGTNFMA
IHTGINYETVKRLKNQGRHGGLVSRGGAFMTAWMLHNEMENPLYRRFDYLVEILKEHEVTLSFGNGMRAGACHDATDRAA
IQELLINAELADQAHNAGVQCILEGPGHIPLDEIKTNVQLEKRVTNNKPFYMLGPLVTDIAPGYDDRVAAIGASVSSAAG
ADFICYVTPAEHLALPTPEEVYEGVMSSRIAAHVGDMVKLPKTREADLEMGHARRDLDWERQYAVSINAEKARCIRNSRM
PADSDACTMCGDFCAIKIVQKTFNF
>Mature_424_residues
TIVEDAKKGLITEEMKVVAKSEGVTEDFIRRGIAGGHIVIPMTPYRKVKLCGIGSGLRTKVNASIGTSSDIVNVEEELEK
ARQAELAGADSLMELSTGGDFLDIRRRVCEQSNLSVGSVPLYQAFIEAARNKGGVVFMDEDDLFKITEQQAKLGTNFMAI
HTGINYETVKRLKNQGRHGGLVSRGGAFMTAWMLHNEMENPLYRRFDYLVEILKEHEVTLSFGNGMRAGACHDATDRAAI
QELLINAELADQAHNAGVQCILEGPGHIPLDEIKTNVQLEKRVTNNKPFYMLGPLVTDIAPGYDDRVAAIGASVSSAAGA
DFICYVTPAEHLALPTPEEVYEGVMSSRIAAHVGDMVKLPKTREADLEMGHARRDLDWERQYAVSINAEKARCIRNSRMP
ADSDACTMCGDFCAIKIVQKTFNF

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=464, Percent_Identity=34.698275862069, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_METLZ (A2SU66)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001031139.1
- STRING:   A2SU66
- GeneID:   4795839
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1711
- eggNOG:   arNOG04546
- HOGENOM:   HBG285234
- OMA:   VPLYQAF
- ProtClustDB:   PRK13352
- BioCyc:   MLAB410358:MLAB_1711-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 46657; Mature: 46526

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: BINDING 94-94 BINDING 123-123 BINDING 162-162 BINDING 264-264 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVEDAKKGLITEEMKVVAKSEGVTEDFIRRGIAGGHIVIPMTPYRKVKLCGIGSGLRT
CCCHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCE
KVNASIGTSSDIVNVEEELEKARQAELAGADSLMELSTGGDFLDIRRRVCEQSNLSVGSV
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
PLYQAFIEAARNKGGVVFMDEDDLFKITEQQAKLGTNFMAIHTGINYETVKRLKNQGRHG
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCC
GLVSRGGAFMTAWMLHNEMENPLYRRFDYLVEILKEHEVTLSFGNGMRAGACHDATDRAA
CCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHH
IQELLINAELADQAHNAGVQCILEGPGHIPLDEIKTNVQLEKRVTNNKPFYMLGPLVTDI
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCEEEEHHCCCCCEEEECCHHHHC
APGYDDRVAAIGASVSSAAGADFICYVTPAEHLALPTPEEVYEGVMSSRIAAHVGDMVKL
CCCCCCHHHHHCCCHHCCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PKTREADLEMGHARRDLDWERQYAVSINAEKARCIRNSRMPADSDACTMCGDFCAIKIVQ
CCCCCCHHHHCCHHCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KTFNF
HHHCC
>Mature Secondary Structure 
TIVEDAKKGLITEEMKVVAKSEGVTEDFIRRGIAGGHIVIPMTPYRKVKLCGIGSGLRT
CCHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCE
KVNASIGTSSDIVNVEEELEKARQAELAGADSLMELSTGGDFLDIRRRVCEQSNLSVGSV
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
PLYQAFIEAARNKGGVVFMDEDDLFKITEQQAKLGTNFMAIHTGINYETVKRLKNQGRHG
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCC
GLVSRGGAFMTAWMLHNEMENPLYRRFDYLVEILKEHEVTLSFGNGMRAGACHDATDRAA
CCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHH
IQELLINAELADQAHNAGVQCILEGPGHIPLDEIKTNVQLEKRVTNNKPFYMLGPLVTDI
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCEEEEHHCCCCCEEEECCHHHHC
APGYDDRVAAIGASVSSAAGADFICYVTPAEHLALPTPEEVYEGVMSSRIAAHVGDMVKL
CCCCCCHHHHHCCCHHCCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PKTREADLEMGHARRDLDWERQYAVSINAEKARCIRNSRMPADSDACTMCGDFCAIKIVQ
CCCCCCHHHHCCHHCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KTFNF
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA