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The map label for this gene is rpoA2 [H]

Identifier: 124485980

GI number: 124485980

Start: 1177812

End: 1179326

Strand: Reverse

Name: rpoA2 [H]

Synonym: Mlab_1160

Alternate gene names: 124485980

Gene position: 1179326-1177812 (Counterclockwise)

Preceding gene: 124485981

Following gene: 124485979

Centisome position: 65.34

GC content: 54.46

Gene sequence:

>1515_bases
ATGGTTCCAAACAATGACGAGTTCGACGAGGAGTTCGATACCTTCGAGGAAGTTATCGAAGAAACTCCCGTAACGACCTC
AAAGAAATCCCTCGCAGAAAAGGTCGAAGCGGTTCTTGAAACGGAATCTGCCGAGGAAACCCCCGCCGAGTCCGCAGAAA
AGAAAACCCGGTCGAAGAAGGCCAAGAAGACCGAAGAGGCCGCAGAAGAGCCCGTGTCACCGATCGCCGCCGAGCTGCAC
AGCTGGGGTCTTCCAAAGACTCTCTTCCAGCAGTTGAAGACCTGGCTAGAGAGCAAATCCGAAGAGGATCTCGAGGCTAT
CGATCTCGCCCGCATGAAGTCCCGTGTCCGGAGACTCGTGGATGAGAACATGCCGGTCTCAACGACCACGGAACTCACGA
AGATCCTTATCTCGAAGTATGATGCAGGCAAGGAGGTAACCGACGCCAACTTCGAAAACATCGTTGACAGAATCAACACC
GAGTACGAACGCACCCGCGTTCAGCCCTGCGAAGCGGTCGGTATCAACGCAGCCCACTCCATAGGTGAACCGGGTACGCA
GATGACGATGCGTACGTTCCACTACGCGGGTGTGGCGGAAATCAACGTTACCCTCGGTCTGCCGCGTCTTATCGAAATCA
TGGACGCGAGAAAAGAGCCGTCCACGCCGACCATGACCATCTTCTTAGATAAGGACTACCGCGAGAGCCGTGATAAGGCC
CGTGAGGTCTCCTGGAAGATCGAAGCCGCACCCCTTCACGAGTTCGGCGACATCGAGACCGACATCGCAGAGATGTGCGT
TCTCGTTCAGGTCAACAAAGAAGTCTGCAAGAAACGTAAGATCGCCGTATCCGAAGTTCTTGCCCGTGCTCCGCAGAAGA
TCAAAGACAAGCGTCACTACCGCGACTTCGAGGTCGAGGTCGATGAGGCGAACGGCGTCTTGAAGTTCCTCCCGAAAAAC
GAGGACTCCTATCAGAATCTCTTCCAGCTCGCAGAACATGTCCGTCAGGTCATCGTTCAGGGTATCGATGATATCAAGAG
GGTCGTCGTTCGAAAGGAAAACGACGAGTACATCCTCCATACGGAAGGTTCCAACCTGAAAGATGTCTTCGAGATTGACG
GCGTCGACTGTACGAGGACCCGTACAAACAACATCTCCGAGATCGCAAGCACGCTCGGTGTCGAGGCCGCACGTTCCGCC
ATCATCTACGAAGCATTCACCACCCTGAAAGAACAGGGTATCTCGGTCGATCTCCGCCACATCATGCTTGTCTCAGACAT
CATGTGCATGGACGGCGAGGTCAAACAGATCGGTCGTCACGGTATTGCCGGCGAGAAGGAATCGGTCCTTTCCCGTGCAA
GCTTCGAAGTTACGGTCAACCACCTGCTGGATGCGGCCGTGGCTCACGAATATGATGAACTTTCAGGTGTCACGGAAAAC
GTTATTGTCGGTCAGCCGATTCTTCTCGGTACCGGCGATGTCAAACTGATGGTCCGCCGCGTGGCACCTCAGTAA

Upstream 100 bases:

>100_bases
GTCTACGGAGAAGACGGTACAGACCCCGCCAAGTCAGCTTCCGGTCTGCCGGTCGATGTGAAAGGTATTGCTGAATCCGT
TCTCAAGGAGGAGATCTAAC

Downstream 100 bases:

>100_bases
TCTTTTTTTTATCATGGTTATTCCTGAACTCTTATCTCCTGCGGGTTCGCCCGATGCTTTGAAAGCCGCTGTATTTGCCG
GGTGCGATGCTGTTTATCTC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MVPNNDEFDEEFDTFEEVIEETPVTTSKKSLAEKVEAVLETESAEETPAESAEKKTRSKKAKKTEEAAEEPVSPIAAELH
SWGLPKTLFQQLKTWLESKSEEDLEAIDLARMKSRVRRLVDENMPVSTTTELTKILISKYDAGKEVTDANFENIVDRINT
EYERTRVQPCEAVGINAAHSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDARKEPSTPTMTIFLDKDYRESRDKA
REVSWKIEAAPLHEFGDIETDIAEMCVLVQVNKEVCKKRKIAVSEVLARAPQKIKDKRHYRDFEVEVDEANGVLKFLPKN
EDSYQNLFQLAEHVRQVIVQGIDDIKRVVVRKENDEYILHTEGSNLKDVFEIDGVDCTRTRTNNISEIASTLGVEAARSA
IIYEAFTTLKEQGISVDLRHIMLVSDIMCMDGEVKQIGRHGIAGEKESVLSRASFEVTVNHLLDAAVAHEYDELSGVTEN
VIVGQPILLGTGDVKLMVRRVAPQ

Sequences:

>Translated_504_residues
MVPNNDEFDEEFDTFEEVIEETPVTTSKKSLAEKVEAVLETESAEETPAESAEKKTRSKKAKKTEEAAEEPVSPIAAELH
SWGLPKTLFQQLKTWLESKSEEDLEAIDLARMKSRVRRLVDENMPVSTTTELTKILISKYDAGKEVTDANFENIVDRINT
EYERTRVQPCEAVGINAAHSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDARKEPSTPTMTIFLDKDYRESRDKA
REVSWKIEAAPLHEFGDIETDIAEMCVLVQVNKEVCKKRKIAVSEVLARAPQKIKDKRHYRDFEVEVDEANGVLKFLPKN
EDSYQNLFQLAEHVRQVIVQGIDDIKRVVVRKENDEYILHTEGSNLKDVFEIDGVDCTRTRTNNISEIASTLGVEAARSA
IIYEAFTTLKEQGISVDLRHIMLVSDIMCMDGEVKQIGRHGIAGEKESVLSRASFEVTVNHLLDAAVAHEYDELSGVTEN
VIVGQPILLGTGDVKLMVRRVAPQ
>Mature_504_residues
MVPNNDEFDEEFDTFEEVIEETPVTTSKKSLAEKVEAVLETESAEETPAESAEKKTRSKKAKKTEEAAEEPVSPIAAELH
SWGLPKTLFQQLKTWLESKSEEDLEAIDLARMKSRVRRLVDENMPVSTTTELTKILISKYDAGKEVTDANFENIVDRINT
EYERTRVQPCEAVGINAAHSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDARKEPSTPTMTIFLDKDYRESRDKA
REVSWKIEAAPLHEFGDIETDIAEMCVLVQVNKEVCKKRKIAVSEVLARAPQKIKDKRHYRDFEVEVDEANGVLKFLPKN
EDSYQNLFQLAEHVRQVIVQGIDDIKRVVVRKENDEYILHTEGSNLKDVFEIDGVDCTRTRTNNISEIASTLGVEAARSA
IIYEAFTTLKEQGISVDLRHIMLVSDIMCMDGEVKQIGRHGIAGEKESVLSRASFEVTVNHLLDAAVAHEYDELSGVTEN
VIVGQPILLGTGDVKLMVRRVAPQ

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=363, Percent_Identity=39.9449035812672, Blast_Score=231, Evalue=2e-60,
Organism=Homo sapiens, GI4505939, Length=418, Percent_Identity=28.7081339712919, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI103471997, Length=146, Percent_Identity=36.986301369863, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25145495, Length=366, Percent_Identity=36.3387978142077, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI71987878, Length=161, Percent_Identity=38.5093167701863, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71998295, Length=65, Percent_Identity=58.4615384615385, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324690, Length=357, Percent_Identity=36.4145658263305, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6320061, Length=178, Percent_Identity=34.2696629213483, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324917, Length=68, Percent_Identity=55.8823529411765, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360912, Length=361, Percent_Identity=38.5041551246537, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17530899, Length=161, Percent_Identity=37.888198757764, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17647875, Length=164, Percent_Identity=34.7560975609756, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 56705; Mature: 56705

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPNNDEFDEEFDTFEEVIEETPVTTSKKSLAEKVEAVLETESAEETPAESAEKKTRSKK
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AKKTEEAAEEPVSPIAAELHSWGLPKTLFQQLKTWLESKSEEDLEAIDLARMKSRVRRLV
HHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DENMPVSTTTELTKILISKYDAGKEVTDANFENIVDRINTEYERTRVQPCEAVGINAAHS
HCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHCCCHHHHCCCCHHH
IGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDARKEPSTPTMTIFLDKDYRESRDKA
CCCCCHHHHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHH
REVSWKIEAAPLHEFGDIETDIAEMCVLVQVNKEVCKKRKIAVSEVLARAPQKIKDKRHY
HHHCEEEEECCCHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHCC
RDFEVEVDEANGVLKFLPKNEDSYQNLFQLAEHVRQVIVQGIDDIKRVVVRKENDEYILH
CCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TEGSNLKDVFEIDGVDCTRTRTNNISEIASTLGVEAARSAIIYEAFTTLKEQGISVDLRH
ECCCCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IMLVSDIMCMDGEVKQIGRHGIAGEKESVLSRASFEVTVNHLLDAAVAHEYDELSGVTEN
HHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VIVGQPILLGTGDVKLMVRRVAPQ
EEECCCEEECCCHHHHHHHHHCCC
>Mature Secondary Structure
MVPNNDEFDEEFDTFEEVIEETPVTTSKKSLAEKVEAVLETESAEETPAESAEKKTRSKK
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AKKTEEAAEEPVSPIAAELHSWGLPKTLFQQLKTWLESKSEEDLEAIDLARMKSRVRRLV
HHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DENMPVSTTTELTKILISKYDAGKEVTDANFENIVDRINTEYERTRVQPCEAVGINAAHS
HCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHCCCHHHHCCCCHHH
IGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDARKEPSTPTMTIFLDKDYRESRDKA
CCCCCHHHHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHH
REVSWKIEAAPLHEFGDIETDIAEMCVLVQVNKEVCKKRKIAVSEVLARAPQKIKDKRHY
HHHCEEEEECCCHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHCC
RDFEVEVDEANGVLKFLPKNEDSYQNLFQLAEHVRQVIVQGIDDIKRVVVRKENDEYILH
CCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TEGSNLKDVFEIDGVDCTRTRTNNISEIASTLGVEAARSAIIYEAFTTLKEQGISVDLRH
ECCCCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IMLVSDIMCMDGEVKQIGRHGIAGEKESVLSRASFEVTVNHLLDAAVAHEYDELSGVTEN
HHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VIVGQPILLGTGDVKLMVRRVAPQ
EEECCCEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]