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The map label for this gene is hycI [H]

Identifier: 124485956

GI number: 124485956

Start: 1150112

End: 1150555

Strand: Reverse

Name: hycI [H]

Synonym: Mlab_1136

Alternate gene names: 124485956

Gene position: 1150555-1150112 (Counterclockwise)

Preceding gene: 124485957

Following gene: 124485955

Centisome position: 63.74

GC content: 51.8

Gene sequence:

>444_bases
ATGAAAGTAGTTCTTCTCGGCGTCGGCAATTCTCTTCACTCCGACGACGGCGCCGGCCCCCGTCTTGCGGAAATGTTTTT
GGATTCACATGATGTCACGTCGTTCAACTGCGGGACCGCCCCTGAGAATTTCACCGGTCTTGTGCGAAAGTTGAACCCTG
ATCTTCTTCTCATCGCCGACGCTGCAGATATGGGCCTTCCTGCGGGTTCAGTCAGGATCATTCCGCCGGAAAAGATCCGC
GACACCGCAATTGGAACCCATATGCTTCCTTTGTATCATCTGGTCGATTTCCTGGCCGAGTCATCGCGCGAAATCTCTAT
CATCGGAATCCAGCCGGCCAGTCTTTCTTGGGGCGACCGGCTGAGCGACCCTGTAGCTTCGTCATTAATCGAAACAAAGA
TGTTGATTGACGAAAACAATCTTCGGAATATCCCTGTTTTGTAA

Upstream 100 bases:

>100_bases
GTTATCACTAGGCGTTGCCAACGCCTAAACTCGGGTTCGAGTCCCGGTCGATGCATCTGTTTTTGGCATCTAAAACTGGT
ATGTTGTTACGAAGGTTGGT

Downstream 100 bases:

>100_bases
ATTCAGGTAAAGATAAAGTTAATCTCTGTCGAACTACAGAGAGTAATACAATCATGTCCGATATAAAGGTCCCCCTCGAC
GTTCCCAAAGCCATGCGGGA

Product: hypothetical protein

Products: NA

Alternate protein names: HycI protease [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MKVVLLGVGNSLHSDDGAGPRLAEMFLDSHDVTSFNCGTAPENFTGLVRKLNPDLLLIADAADMGLPAGSVRIIPPEKIR
DTAIGTHMLPLYHLVDFLAESSREISIIGIQPASLSWGDRLSDPVASSLIETKMLIDENNLRNIPVL

Sequences:

>Translated_147_residues
MKVVLLGVGNSLHSDDGAGPRLAEMFLDSHDVTSFNCGTAPENFTGLVRKLNPDLLLIADAADMGLPAGSVRIIPPEKIR
DTAIGTHMLPLYHLVDFLAESSREISIIGIQPASLSWGDRLSDPVASSLIETKMLIDENNLRNIPVL
>Mature_147_residues
MKVVLLGVGNSLHSDDGAGPRLAEMFLDSHDVTSFNCGTAPENFTGLVRKLNPDLLLIADAADMGLPAGSVRIIPPEKIR
DTAIGTHMLPLYHLVDFLAESSREISIIGIQPASLSWGDRLSDPVASSLIETKMLIDENNLRNIPVL

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3 [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=132, Percent_Identity=40.9090909090909, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: =3.4.23.51 [H]

Molecular weight: Translated: 15827; Mature: 15827

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLLGVGNSLHSDDGAGPRLAEMFLDSHDVTSFNCGTAPENFTGLVRKLNPDLLLIAD
CEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEEC
AADMGLPAGSVRIIPPEKIRDTAIGTHMLPLYHLVDFLAESSREISIIGIQPASLSWGDR
CCCCCCCCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
LSDPVASSLIETKMLIDENNLRNIPVL
CCCHHHHHHHHHHHEECCCCCCCCCCC
>Mature Secondary Structure
MKVVLLGVGNSLHSDDGAGPRLAEMFLDSHDVTSFNCGTAPENFTGLVRKLNPDLLLIAD
CEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEEC
AADMGLPAGSVRIIPPEKIRDTAIGTHMLPLYHLVDFLAESSREISIIGIQPASLSWGDR
CCCCCCCCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
LSDPVASSLIETKMLIDENNLRNIPVL
CCCHHHHHHHHHHHEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]