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The map label for this gene is capI [H]

Identifier: 124485719

GI number: 124485719

Start: 888306

End: 889319

Strand: Reverse

Name: capI [H]

Synonym: Mlab_0897

Alternate gene names: 124485719

Gene position: 889319-888306 (Counterclockwise)

Preceding gene: 124485723

Following gene: 124485718

Centisome position: 49.27

GC content: 42.01

Gene sequence:

>1014_bases
ATGTCATTAGATACGATACTCATTACCGGGGCTGCCGGGTTTATTGGCTTTCATTTATCCAAAAAACTCCTGGAACAGGG
AATCCAGGTTATCGGCTACGATAATATCAACTCCTATTATGATGTCAACTTAAAATATGCACGTCTCGCTATCCTAAAGG
ACTATCCTGATTTTATTTTTGTAAAGGGCGATTTGGCTGATAAATCAGAGGTAGAGAATGTATTTACTAAATATAAACCA
GATATTGTTGTGAATCTGGCTGCACAGGCAGGTGTTCGCTACTCTATCGACAATCCTCAGGTGTATATCGATAGTAACAT
AATTGGATTTTTCAATATTCTGGAAGCTTGCCGTCACCACCCGGCTGAGCATCTGATATATGCATCCAGCTCTTCTGTGT
ATGGAAATCAGGAGAAGACTCCATTTTCAACGGATGATGATGTAAGCAGGCCAATCAGTCTGTATGCGGCAACCAAAAAA
AGCAATGAACTGATGGCATATACATATAGTCATCTTTATGGTATCCCGACAACCGGTCTTCGTTTCTTTACTGTATATGG
TCCTTATGGGCGTCCGGATATGGCATACTTTAGCTTTACCAGGAAGATTCTTGCCGGTGAGACCATTCAGATCTTCAATA
ATGGAGATATGTACCGGGACTTTACGTATATAGATGATATCGTTCAGGGAATAGAAAATATGCTTGAGCACCCACCCGCA
GCAGATGAAAATGGTGATCGCTACAAGTTGTATAATATTGGTAATAATCACCCGGAAAAGCTGATGTACTTCATCGAGGT
GCTGGAAAAGTGCATCGGGAGAGAAGCGAAGAAAGAGTTCTTGCCGATGCAGCCGGGTGATGTGTATCAGACATATGCTG
ATGTGGATGATTTGGTGTGGGATTTTGGATTTAAGCCGGAGACGAGTGTCGAGGTGGGGCTGGGGAAATTTGTGGAGTGG
TATAAGAAATACTTTATACCTCAAGTCTGCCCAATCGTCTATTCTGCGAAATAA

Upstream 100 bases:

>100_bases
ATTATACGTCTTTTTATCATAGAACAAAGTATATATTTCTTATCAGGGATAATCTTTATCCAAAGAATAATGGGTATATA
ACCTGAATGTATATGATGTA

Downstream 100 bases:

>100_bases
ACCTCTCCATTTTTTCATATGTAGACTAATTTTTCTGAGGGTGGCGTGTTATTATTTCAAGATGAATCCAAAGGTATTTT
CAAAAATAGGTTCATTCAAC

Product: hypothetical protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSLDTILITGAAGFIGFHLSKKLLEQGIQVIGYDNINSYYDVNLKYARLAILKDYPDFIFVKGDLADKSEVENVFTKYKP
DIVVNLAAQAGVRYSIDNPQVYIDSNIIGFFNILEACRHHPAEHLIYASSSSVYGNQEKTPFSTDDDVSRPISLYAATKK
SNELMAYTYSHLYGIPTTGLRFFTVYGPYGRPDMAYFSFTRKILAGETIQIFNNGDMYRDFTYIDDIVQGIENMLEHPPA
ADENGDRYKLYNIGNNHPEKLMYFIEVLEKCIGREAKKEFLPMQPGDVYQTYADVDDLVWDFGFKPETSVEVGLGKFVEW
YKKYFIPQVCPIVYSAK

Sequences:

>Translated_337_residues
MSLDTILITGAAGFIGFHLSKKLLEQGIQVIGYDNINSYYDVNLKYARLAILKDYPDFIFVKGDLADKSEVENVFTKYKP
DIVVNLAAQAGVRYSIDNPQVYIDSNIIGFFNILEACRHHPAEHLIYASSSSVYGNQEKTPFSTDDDVSRPISLYAATKK
SNELMAYTYSHLYGIPTTGLRFFTVYGPYGRPDMAYFSFTRKILAGETIQIFNNGDMYRDFTYIDDIVQGIENMLEHPPA
ADENGDRYKLYNIGNNHPEKLMYFIEVLEKCIGREAKKEFLPMQPGDVYQTYADVDDLVWDFGFKPETSVEVGLGKFVEW
YKKYFIPQVCPIVYSAK
>Mature_336_residues
SLDTILITGAAGFIGFHLSKKLLEQGIQVIGYDNINSYYDVNLKYARLAILKDYPDFIFVKGDLADKSEVENVFTKYKPD
IVVNLAAQAGVRYSIDNPQVYIDSNIIGFFNILEACRHHPAEHLIYASSSSVYGNQEKTPFSTDDDVSRPISLYAATKKS
NELMAYTYSHLYGIPTTGLRFFTVYGPYGRPDMAYFSFTRKILAGETIQIFNNGDMYRDFTYIDDIVQGIENMLEHPPAA
DENGDRYKLYNIGNNHPEKLMYFIEVLEKCIGREAKKEFLPMQPGDVYQTYADVDDLVWDFGFKPETSVEVGLGKFVEWY
KKYFIPQVCPIVYSAK

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=327, Percent_Identity=26.2996941896024, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=25.7575757575758, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56237023, Length=343, Percent_Identity=23.6151603498542, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI56118217, Length=343, Percent_Identity=23.6151603498542, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI189083684, Length=343, Percent_Identity=23.6151603498542, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI48994969, Length=342, Percent_Identity=28.9473684210526, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=26.530612244898, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1786974, Length=345, Percent_Identity=23.768115942029, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=26.628895184136, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788589, Length=272, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=346, Percent_Identity=26.0115606936416, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=348, Percent_Identity=25.8620689655172, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=327, Percent_Identity=26.605504587156, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=25.8064516129032, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=323, Percent_Identity=22.6006191950464, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=342, Percent_Identity=25.1461988304094, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19923002, Length=335, Percent_Identity=25.3731343283582, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=25.3822629969419, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38466; Mature: 38335

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDTILITGAAGFIGFHLSKKLLEQGIQVIGYDNINSYYDVNLKYARLAILKDYPDFIF
CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCEEECCEEEEEEEEEECCCCEEE
VKGDLADKSEVENVFTKYKPDIVVNLAAQAGVRYSIDNPQVYIDSNIIGFFNILEACRHH
EECCCCCHHHHHHHHHHCCCCEEEEEHHHCCEEEECCCCEEEECCCCHHHHHHHHHHHCC
PAEHLIYASSSSVYGNQEKTPFSTDDDVSRPISLYAATKKSNELMAYTYSHLYGIPTTGL
CHHHEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEHHHHCCCCCCCE
RFFTVYGPYGRPDMAYFSFTRKILAGETIQIFNNGDMYRDFTYIDDIVQGIENMLEHPPA
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCC
ADENGDRYKLYNIGNNHPEKLMYFIEVLEKCIGREAKKEFLPMQPGDVYQTYADVDDLVW
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHH
DFGFKPETSVEVGLGKFVEWYKKYFIPQVCPIVYSAK
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCEEEECC
>Mature Secondary Structure 
SLDTILITGAAGFIGFHLSKKLLEQGIQVIGYDNINSYYDVNLKYARLAILKDYPDFIF
CCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCEEECCEEEEEEEEEECCCCEEE
VKGDLADKSEVENVFTKYKPDIVVNLAAQAGVRYSIDNPQVYIDSNIIGFFNILEACRHH
EECCCCCHHHHHHHHHHCCCCEEEEEHHHCCEEEECCCCEEEECCCCHHHHHHHHHHHCC
PAEHLIYASSSSVYGNQEKTPFSTDDDVSRPISLYAATKKSNELMAYTYSHLYGIPTTGL
CHHHEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEHHHHCCCCCCCE
RFFTVYGPYGRPDMAYFSFTRKILAGETIQIFNNGDMYRDFTYIDDIVQGIENMLEHPPA
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCC
ADENGDRYKLYNIGNNHPEKLMYFIEVLEKCIGREAKKEFLPMQPGDVYQTYADVDDLVW
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHH
DFGFKPETSVEVGLGKFVEWYKKYFIPQVCPIVYSAK
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]