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The map label for this gene is thiI [H]

Identifier: 124485194

GI number: 124485194

Start: 344862

End: 346076

Strand: Reverse

Name: thiI [H]

Synonym: Mlab_0367

Alternate gene names: 124485194

Gene position: 346076-344862 (Counterclockwise)

Preceding gene: 124485195

Following gene: 124485193

Centisome position: 19.17

GC content: 52.26

Gene sequence:

>1215_bases
ATGGAACAAAATAGAGCAGTAATGGTCAGGATCGGGGAGCTTTGGCTCAAAAGCGAGCCGGTCAAAAAACAGTTCATGCT
TGCGCTAACGCGAAATATCAAAGCAGCTCTGGATACGCAGGAAATCCCATACATGTTAGAGGAATACCGGGGAAGACTGC
TGATCTTCGGCGATGCCGCAAGAATCGCCCCGGTCGTAGCGAGGATATTTGGAATCGTTGACGTCAGTATCTGCGAAACG
ACCACAAACCGTCCCGAAGACATGGCAAAAACCGCCCTGACGTTTTCTGAGAAAAAACTCAAATCCGGTATGCGTTTTGC
GGTCAGAGCGCGACGCCAGCATGTGAGCGGATTCACCAGTCAGCAGCTTGCCGGCATGATCGCCGATGCCATTTGGGAGA
AGATCCCGGATTTCGTTGTCGATCTCGATGACCCGGAGTATGAAATATTCGTCGAGGCACGGGAATACGGTGGAATCGTA
TATGATGAGAGAATTCCAGGACAAGGCGGACTTCCCCTTGGAACAGCAGGCCGGGCAGTAGCGCTTCTTTCCGCAGGCAT
CGATTCGCCCGTTGCCGCATGGCTGATGATGAGGAGAGGCGTAGTCATCTCCGGTGTATTTATGGATGGGGGAAGATGGG
CGGGATCTGCCACGCGTGACTTGGCAATGGATAATGTCAGAATTCTCTCCACCTGGTGTCCCGGAAGGGGTCTGCCGCTT
TGGATCGTGAATCTCGAGCCGTTCTTCGATGCGATGATGACTGCATGTGACCGACATTACACCTGCCTATTCTGTAAGAG
ATTCATGATGCGGGTGGCTGAAGAGGTGGCGAAAGAGAATAAAATGGAGGGGATCGTCTCCGGAGAAAATCTCGGGCAGG
TGGCATCACAAACTTTGCAGAATATGGGCGTCATCACCGAATCGGTGAAGCTTCCAGTTTTGCGCCCTCTCCTGACCTAT
GATAAAGAGGAGATCGTAGCGATCTCACGAAGAATCGGCACCTATCACGAAAGCCCAGGCGACACGGGGTGCCTCGCGGT
CCCCAAAAAACCGGCAACCCGCTCCGCACAGGATCTGATCGACACTGAAGAAAACAAGCTCGAGATGAATGAGCTCGTTC
GGCAGGCCGTTGAATCAGCAGAGCTTTGGATCGCCAAAGACGGAGAGATCTTCCAAAAAATACTCACTGAGAGAACGATC
GATTCTTCCGTTTAA

Upstream 100 bases:

>100_bases
AAAATGAGATGGCCAATGCAGAATTATATGCAGATGATCTGAAGGCCTTCGGGTTTTCCACAATATCGGTCTCAGAATAC
AAAACAGGAGGTTCACAGAC

Downstream 100 bases:

>100_bases
CAACACACAATCTATTTTTACCACAACGACAAATATCATAAGGTCCGGAGCCGAGATAGTCTAGTCCGGAAAGGCGGTGG
CCTTGAAAGCCTCTGGTAGT

Product: hypothetical protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MEQNRAVMVRIGELWLKSEPVKKQFMLALTRNIKAALDTQEIPYMLEEYRGRLLIFGDAARIAPVVARIFGIVDVSICET
TTNRPEDMAKTALTFSEKKLKSGMRFAVRARRQHVSGFTSQQLAGMIADAIWEKIPDFVVDLDDPEYEIFVEAREYGGIV
YDERIPGQGGLPLGTAGRAVALLSAGIDSPVAAWLMMRRGVVISGVFMDGGRWAGSATRDLAMDNVRILSTWCPGRGLPL
WIVNLEPFFDAMMTACDRHYTCLFCKRFMMRVAEEVAKENKMEGIVSGENLGQVASQTLQNMGVITESVKLPVLRPLLTY
DKEEIVAISRRIGTYHESPGDTGCLAVPKKPATRSAQDLIDTEENKLEMNELVRQAVESAELWIAKDGEIFQKILTERTI
DSSV

Sequences:

>Translated_404_residues
MEQNRAVMVRIGELWLKSEPVKKQFMLALTRNIKAALDTQEIPYMLEEYRGRLLIFGDAARIAPVVARIFGIVDVSICET
TTNRPEDMAKTALTFSEKKLKSGMRFAVRARRQHVSGFTSQQLAGMIADAIWEKIPDFVVDLDDPEYEIFVEAREYGGIV
YDERIPGQGGLPLGTAGRAVALLSAGIDSPVAAWLMMRRGVVISGVFMDGGRWAGSATRDLAMDNVRILSTWCPGRGLPL
WIVNLEPFFDAMMTACDRHYTCLFCKRFMMRVAEEVAKENKMEGIVSGENLGQVASQTLQNMGVITESVKLPVLRPLLTY
DKEEIVAISRRIGTYHESPGDTGCLAVPKKPATRSAQDLIDTEENKLEMNELVRQAVESAELWIAKDGEIFQKILTERTI
DSSV
>Mature_404_residues
MEQNRAVMVRIGELWLKSEPVKKQFMLALTRNIKAALDTQEIPYMLEEYRGRLLIFGDAARIAPVVARIFGIVDVSICET
TTNRPEDMAKTALTFSEKKLKSGMRFAVRARRQHVSGFTSQQLAGMIADAIWEKIPDFVVDLDDPEYEIFVEAREYGGIV
YDERIPGQGGLPLGTAGRAVALLSAGIDSPVAAWLMMRRGVVISGVFMDGGRWAGSATRDLAMDNVRILSTWCPGRGLPL
WIVNLEPFFDAMMTACDRHYTCLFCKRFMMRVAEEVAKENKMEGIVSGENLGQVASQTLQNMGVITESVKLPVLRPLLTY
DKEEIVAISRRIGTYHESPGDTGCLAVPKKPATRSAQDLIDTEENKLEMNELVRQAVESAELWIAKDGEIFQKILTERTI
DSSV

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=386, Percent_Identity=27.720207253886, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 45060; Mature: 45060

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQNRAVMVRIGELWLKSEPVKKQFMLALTRNIKAALDTQEIPYMLEEYRGRLLIFGDAA
CCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHCHHHHHHHCCCEEEEECHH
RIAPVVARIFGIVDVSICETTTNRPEDMAKTALTFSEKKLKSGMRFAVRARRQHVSGFTS
HHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QQLAGMIADAIWEKIPDFVVDLDDPEYEIFVEAREYGGIVYDERIPGQGGLPLGTAGRAV
HHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEHHHCCEEEECCCCCCCCCCCCCCCHHH
ALLSAGIDSPVAAWLMMRRGVVISGVFMDGGRWAGSATRDLAMDNVRILSTWCPGRGLPL
HHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHCCEEEEEEECCCCCCEE
WIVNLEPFFDAMMTACDRHYTCLFCKRFMMRVAEEVAKENKMEGIVSGENLGQVASQTLQ
EEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
NMGVITESVKLPVLRPLLTYDKEEIVAISRRIGTYHESPGDTGCLAVPKKPATRSAQDLI
HCCHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHH
DTEENKLEMNELVRQAVESAELWIAKDGEIFQKILTERTIDSSV
CCCCHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEQNRAVMVRIGELWLKSEPVKKQFMLALTRNIKAALDTQEIPYMLEEYRGRLLIFGDAA
CCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHCHHHHHHHCCCEEEEECHH
RIAPVVARIFGIVDVSICETTTNRPEDMAKTALTFSEKKLKSGMRFAVRARRQHVSGFTS
HHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QQLAGMIADAIWEKIPDFVVDLDDPEYEIFVEAREYGGIVYDERIPGQGGLPLGTAGRAV
HHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEHHHCCEEEECCCCCCCCCCCCCCCHHH
ALLSAGIDSPVAAWLMMRRGVVISGVFMDGGRWAGSATRDLAMDNVRILSTWCPGRGLPL
HHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHCCEEEEEEECCCCCCEE
WIVNLEPFFDAMMTACDRHYTCLFCKRFMMRVAEEVAKENKMEGIVSGENLGQVASQTLQ
EEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
NMGVITESVKLPVLRPLLTYDKEEIVAISRRIGTYHESPGDTGCLAVPKKPATRSAQDLI
HCCHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHH
DTEENKLEMNELVRQAVESAELWIAKDGEIFQKILTERTIDSSV
CCCCHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]