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The map label for this gene is pbp [H]

Identifier: 124485162

GI number: 124485162

Start: 315824

End: 317425

Strand: Reverse

Name: pbp [H]

Synonym: Mlab_0335

Alternate gene names: 124485162

Gene position: 317425-315824 (Counterclockwise)

Preceding gene: 124485163

Following gene: 124485161

Centisome position: 17.59

GC content: 49.5

Gene sequence:

>1602_bases
ATGATCTCTTTGCTTCGTTTTCTGTGTACACTAAAAGGAGGCAGGATACCCGGCATAAGTCTGGCAGTCCTCCTCCTCTG
TTTTTGTCTAGTCTGCGTGCCGGTTTTGGCTGATTCTGATCTCGGCCCGATACCTGACGCTCGTTCAAATCCCGCAGTAA
GTGCTGATGTCGAGGCATTTTTTGACGCATCGATCCCGGCAGAACTCGCAAAATACAATATACCCGGCGCGACGGTCGCA
GCAGTATATGATGGAAAACTGATCTTTTCCAAAGGATACGGGTATGCTGATATCGCCAACAGAACACCGGTCGATGCTGG
ATCGACACTCTTCCATATTGGTTCGGTCACGAAACTTTTCACCTGGACATGTGTTATGCAGCTGGTGGACGAGGGAAAAA
TAGACCTGGATGCAGACATCAACACCTACCTTGCCGATTTTTCTCTTCCGGACACCTACCCCGGCCAGCCGGTCACTATG
CGAAACCTGATGACGCACACTGCCGGATTTGAAGACGCAATCCGGAGGGCTACAGTTGAAGACGTAGATGATCTTTTCTC
ATTCAGGATCTACTGCCGGGATTATATACCTGCACGGGTCTATCCTCCGGGAACCGTGACCTCCTACTCAAATTACGGCA
CAACGCTTGCAGCCGTCATTGTTGAGGATGTAAGCGGCATGCCGTTTGAACAGTATCTCCAGGAACATATCTTAACCCCG
CTTGATCTCAAAAATACCCATCTATCCTATCTCCTCCCGCCAGAATCCGCCGGCAGCCTCTCCTCCGGATATAATTATAT
CGGCGGGACAAATGCAGCGATCACCGACACGATTTTTGTCATCGGACCTGCCGGATCCATCAGCTCCACAGCAGAGGATA
TGGCAGTTTTCCTTGCCGCACATATGCAGAACGGAAAAGTGAACGGCGCAGAAATTCTTTCTGAACCGGCCGCCGCACTC
ATGCATGCAAGAGCATTTACAAATGACCCGCGGGTCAGCGGCATCTGCCTTGGATTTTATGAAAACTATCTCAATGGCGA
GCGGATCATCCTCCACGGCGGAGATACCAATACCTTCCATTCGCTTTTCGTTATCATTCCTGACAAAAAAACCGGGTTTT
TTGTCTCATACAATGCTCCCGGAGGGACAAGTGCACGAAATGATCTGCTTCTCGAGTATGTCGGCCGATTCTTCCCCCTA
ACGGAGACGGAGACGGTGACGCAGGAAATAGAACATTCCGGATCATTTGAGAAGTATACAGGAGTTTATCAGTCAAACCG
CCATAATTACCGGACGTTTGAATTTTTCCTTCAACCTCCGCAGCAAATGACGGTTGAAGCGGGAGAAAATAATGCCTGCC
TTGTGTTAAATATTGATGGCGACTCTACAGAATACATCGAGGTTTCACCCGGCGTCTTTCAAGAGAAAAGCGGCGGCCCG
TCAGTGTATGGAAATATGGTCTTTCGGGAGAATGAGAACGGTGATGTGACGTTCCTCTGTTCCGAAAATACGCCGGTGCT
CGCATATGAACGGGTCCCATGGTATTCTGTCAATACGTTTACGTCCGGGCTCCTCTCTGCTTTGAATGGTTCAACAACAT
GA

Upstream 100 bases:

>100_bases
AACGAAACCCCGCATCTCAGTTTTGGGAGTGAACGGCAAAACCGCTGATTCGAAAAAAAACATAAATTCAAATAAAAAGC
AGTGAGATAGTATCAGTTAG

Downstream 100 bases:

>100_bases
ACCTGTCAGGCAAACCAGAAGAGGCAAAGTTTATGTACGCTGCCTGGCTGAGCGGCATTATGTCGAATGGGGAACCCGCG
TTCGCTTCCCAGTGTATTCA

Product: hypothetical protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP-4 [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MISLLRFLCTLKGGRIPGISLAVLLLCFCLVCVPVLADSDLGPIPDARSNPAVSADVEAFFDASIPAELAKYNIPGATVA
AVYDGKLIFSKGYGYADIANRTPVDAGSTLFHIGSVTKLFTWTCVMQLVDEGKIDLDADINTYLADFSLPDTYPGQPVTM
RNLMTHTAGFEDAIRRATVEDVDDLFSFRIYCRDYIPARVYPPGTVTSYSNYGTTLAAVIVEDVSGMPFEQYLQEHILTP
LDLKNTHLSYLLPPESAGSLSSGYNYIGGTNAAITDTIFVIGPAGSISSTAEDMAVFLAAHMQNGKVNGAEILSEPAAAL
MHARAFTNDPRVSGICLGFYENYLNGERIILHGGDTNTFHSLFVIIPDKKTGFFVSYNAPGGTSARNDLLLEYVGRFFPL
TETETVTQEIEHSGSFEKYTGVYQSNRHNYRTFEFFLQPPQQMTVEAGENNACLVLNIDGDSTEYIEVSPGVFQEKSGGP
SVYGNMVFRENENGDVTFLCSENTPVLAYERVPWYSVNTFTSGLLSALNGSTT

Sequences:

>Translated_533_residues
MISLLRFLCTLKGGRIPGISLAVLLLCFCLVCVPVLADSDLGPIPDARSNPAVSADVEAFFDASIPAELAKYNIPGATVA
AVYDGKLIFSKGYGYADIANRTPVDAGSTLFHIGSVTKLFTWTCVMQLVDEGKIDLDADINTYLADFSLPDTYPGQPVTM
RNLMTHTAGFEDAIRRATVEDVDDLFSFRIYCRDYIPARVYPPGTVTSYSNYGTTLAAVIVEDVSGMPFEQYLQEHILTP
LDLKNTHLSYLLPPESAGSLSSGYNYIGGTNAAITDTIFVIGPAGSISSTAEDMAVFLAAHMQNGKVNGAEILSEPAAAL
MHARAFTNDPRVSGICLGFYENYLNGERIILHGGDTNTFHSLFVIIPDKKTGFFVSYNAPGGTSARNDLLLEYVGRFFPL
TETETVTQEIEHSGSFEKYTGVYQSNRHNYRTFEFFLQPPQQMTVEAGENNACLVLNIDGDSTEYIEVSPGVFQEKSGGP
SVYGNMVFRENENGDVTFLCSENTPVLAYERVPWYSVNTFTSGLLSALNGSTT
>Mature_533_residues
MISLLRFLCTLKGGRIPGISLAVLLLCFCLVCVPVLADSDLGPIPDARSNPAVSADVEAFFDASIPAELAKYNIPGATVA
AVYDGKLIFSKGYGYADIANRTPVDAGSTLFHIGSVTKLFTWTCVMQLVDEGKIDLDADINTYLADFSLPDTYPGQPVTM
RNLMTHTAGFEDAIRRATVEDVDDLFSFRIYCRDYIPARVYPPGTVTSYSNYGTTLAAVIVEDVSGMPFEQYLQEHILTP
LDLKNTHLSYLLPPESAGSLSSGYNYIGGTNAAITDTIFVIGPAGSISSTAEDMAVFLAAHMQNGKVNGAEILSEPAAAL
MHARAFTNDPRVSGICLGFYENYLNGERIILHGGDTNTFHSLFVIIPDKKTGFFVSYNAPGGTSARNDLLLEYVGRFFPL
TETETVTQEIEHSGSFEKYTGVYQSNRHNYRTFEFFLQPPQQMTVEAGENNACLVLNIDGDSTEYIEVSPGVFQEKSGGP
SVYGNMVFRENENGDVTFLCSENTPVLAYERVPWYSVNTFTSGLLSALNGSTT

Specific function: Involved in cell wall biosynthesis and may also act as a sensor of external penicillins [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1790593, Length=289, Percent_Identity=27.681660899654, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786574, Length=321, Percent_Identity=27.1028037383178, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI115533026, Length=206, Percent_Identity=29.6116504854369, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17550934, Length=259, Percent_Identity=27.4131274131274, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 58025; Mature: 58025

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLLRFLCTLKGGRIPGISLAVLLLCFCLVCVPVLADSDLGPIPDARSNPAVSADVEAF
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
FDASIPAELAKYNIPGATVAAVYDGKLIFSKGYGYADIANRTPVDAGSTLFHIGSVTKLF
CCCCCCHHHHHCCCCCCEEEEEECCEEEEECCCCCEECCCCCCCCCCCCEEECCCHHHHH
TWTCVMQLVDEGKIDLDADINTYLADFSLPDTYPGQPVTMRNLMTHTAGFEDAIRRATVE
HHHHHHHHHHCCCEEECCCCHHEEEECCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHH
DVDDLFSFRIYCRDYIPARVYPPGTVTSYSNYGTTLAAVIVEDVSGMPFEQYLQEHILTP
HHHHHHHHHHHEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCC
LDLKNTHLSYLLPPESAGSLSSGYNYIGGTNAAITDTIFVIGPAGSISSTAEDMAVFLAA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHH
HMQNGKVNGAEILSEPAAALMHARAFTNDPRVSGICLGFYENYLNGERIILHGGDTNTFH
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCEEEEECCCCCCEE
SLFVIIPDKKTGFFVSYNAPGGTSARNDLLLEYVGRFFPLTETETVTQEIEHSGSFEKYT
EEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
GVYQSNRHNYRTFEFFLQPPQQMTVEAGENNACLVLNIDGDSTEYIEVSPGVFQEKSGGP
CHHHCCCCCCEEEEEEECCCHHEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCC
SVYGNMVFRENENGDVTFLCSENTPVLAYERVPWYSVNTFTSGLLSALNGSTT
CEEEEEEEEECCCCCEEEEECCCCCEEEEECCCCEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MISLLRFLCTLKGGRIPGISLAVLLLCFCLVCVPVLADSDLGPIPDARSNPAVSADVEAF
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
FDASIPAELAKYNIPGATVAAVYDGKLIFSKGYGYADIANRTPVDAGSTLFHIGSVTKLF
CCCCCCHHHHHCCCCCCEEEEEECCEEEEECCCCCEECCCCCCCCCCCCEEECCCHHHHH
TWTCVMQLVDEGKIDLDADINTYLADFSLPDTYPGQPVTMRNLMTHTAGFEDAIRRATVE
HHHHHHHHHHCCCEEECCCCHHEEEECCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHH
DVDDLFSFRIYCRDYIPARVYPPGTVTSYSNYGTTLAAVIVEDVSGMPFEQYLQEHILTP
HHHHHHHHHHHEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCC
LDLKNTHLSYLLPPESAGSLSSGYNYIGGTNAAITDTIFVIGPAGSISSTAEDMAVFLAA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHH
HMQNGKVNGAEILSEPAAALMHARAFTNDPRVSGICLGFYENYLNGERIILHGGDTNTFH
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCEEEEECCCCCCEE
SLFVIIPDKKTGFFVSYNAPGGTSARNDLLLEYVGRFFPLTETETVTQEIEHSGSFEKYT
EEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
GVYQSNRHNYRTFEFFLQPPQQMTVEAGENNACLVLNIDGDSTEYIEVSPGVFQEKSGGP
CHHHCCCCCCEEEEEEECCCHHEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCC
SVYGNMVFRENENGDVTFLCSENTPVLAYERVPWYSVNTFTSGLLSALNGSTT
CEEEEEEEEECCCCCEEEEECCCCCEEEEECCCCEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8440253 [H]