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The map label for this gene is thiE [H]

Identifier: 124485103

GI number: 124485103

Start: 256038

End: 256676

Strand: Reverse

Name: thiE [H]

Synonym: Mlab_0276

Alternate gene names: 124485103

Gene position: 256676-256038 (Counterclockwise)

Preceding gene: 124485104

Following gene: 124485102

Centisome position: 14.22

GC content: 44.29

Gene sequence:

>639_bases
GTGACGGCAAACAATTTTGGGTTGTATCTGATCATCACCAAACCCTCTTTTTCCTACAGGAAAATCGCCGAAACCGCGGT
TAAGTATAATGTGCAGTATCTTCAACTGCGGGAGAAATCGTTATCCGACCGGGAGATCCTGAAAGCAGCTGACGAAATCA
TGCAGGTGACAAACGGAACTGGAACTAAATTTATACTGAATGACAGGGCGGATCTGGCATTTATCTGCGGGGCAGACGGT
CTGCATCTTGGTCAGGATGACATATCTCTCTGCGATGCAAAAAAAATCTGCGGCGAAAAAGTATTCAGATTCGGGCTCTC
GACCCATAATCTTGAACAGGTAAAAGAAGCGGTACAACTAAAACCGGATTACATTGGATTTGGGCCAATATTTACGACCC
CGACAAAAGCAAAACCGGATCCGGTAGTTGGAACTGAAATGATTCCAGAAGCAGTTAAATTAGCTGGAGATATCCCGGTT
GTTGCGATCGGCGGAATCGATGGAGAAAACCTCATAAGTGTTCTTGACGCAGGCGCAAGAAATGTGTGTATGGTCAGGTA
TTTTATGAACAGTATGCATTTTGAGACAAGAGTAAAAGAGACGGTAAAGATCCTGAACGATTACGGGATATGTGGATAA

Upstream 100 bases:

>100_bases
CAGCAAACGGAGTGTTTGGATCGCCCCGTATGGGACCGATCTTCGGTGGGATGCTTCTTTCAGGAGAGAAAGTGGCAAAA
CTGATTATCGAGGAGCTGAA

Downstream 100 bases:

>100_bases
GAAAATCAAAAAAAAACAGTGCTGCGAGAGGGATCCGAACCCCCGACCTCGGGATTATGAGTCCCGCGCCCTAACCAGCT
AGGCCACCGCAGCAAATATG

Product: hypothetical protein

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTANNFGLYLIITKPSFSYRKIAETAVKYNVQYLQLREKSLSDREILKAADEIMQVTNGTGTKFILNDRADLAFICGADG
LHLGQDDISLCDAKKICGEKVFRFGLSTHNLEQVKEAVQLKPDYIGFGPIFTTPTKAKPDPVVGTEMIPEAVKLAGDIPV
VAIGGIDGENLISVLDAGARNVCMVRYFMNSMHFETRVKETVKILNDYGICG

Sequences:

>Translated_212_residues
MTANNFGLYLIITKPSFSYRKIAETAVKYNVQYLQLREKSLSDREILKAADEIMQVTNGTGTKFILNDRADLAFICGADG
LHLGQDDISLCDAKKICGEKVFRFGLSTHNLEQVKEAVQLKPDYIGFGPIFTTPTKAKPDPVVGTEMIPEAVKLAGDIPV
VAIGGIDGENLISVLDAGARNVCMVRYFMNSMHFETRVKETVKILNDYGICG
>Mature_211_residues
TANNFGLYLIITKPSFSYRKIAETAVKYNVQYLQLREKSLSDREILKAADEIMQVTNGTGTKFILNDRADLAFICGADGL
HLGQDDISLCDAKKICGEKVFRFGLSTHNLEQVKEAVQLKPDYIGFGPIFTTPTKAKPDPVVGTEMIPEAVKLAGDIPVV
AIGGIDGENLISVLDAGARNVCMVRYFMNSMHFETRVKETVKILNDYGICG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=200, Percent_Identity=29, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=184, Percent_Identity=29.8913043478261, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23329; Mature: 23198

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANNFGLYLIITKPSFSYRKIAETAVKYNVQYLQLREKSLSDREILKAADEIMQVTNGT
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHHCCC
GTKFILNDRADLAFICGADGLHLGQDDISLCDAKKICGEKVFRFGLSTHNLEQVKEAVQL
CCEEEEECCCCEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
KPDYIGFGPIFTTPTKAKPDPVVGTEMIPEAVKLAGDIPVVAIGGIDGENLISVLDAGAR
CCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCC
NVCMVRYFMNSMHFETRVKETVKILNDYGICG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TANNFGLYLIITKPSFSYRKIAETAVKYNVQYLQLREKSLSDREILKAADEIMQVTNGT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHHCCC
GTKFILNDRADLAFICGADGLHLGQDDISLCDAKKICGEKVFRFGLSTHNLEQVKEAVQL
CCEEEEECCCCEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
KPDYIGFGPIFTTPTKAKPDPVVGTEMIPEAVKLAGDIPVVAIGGIDGENLISVLDAGAR
CCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCC
NVCMVRYFMNSMHFETRVKETVKILNDYGICG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA