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The map label for this gene is purA [H]

Identifier: 124484893

GI number: 124484893

Start: 63150

End: 64160

Strand: Reverse

Name: purA [H]

Synonym: Mlab_0064

Alternate gene names: 124484893

Gene position: 64160-63150 (Counterclockwise)

Preceding gene: 124484895

Following gene: 124484892

Centisome position: 3.55

GC content: 53.41

Gene sequence:

>1011_bases
ATGCCAAGTACGATCATAGTCGGCGGCTTTTTTGGCGACGAAGGGAAAGGGAAAATCGTCGCCCACATTGCGCAAAAAGA
CCAAGCAACCATCATTGCCCGCGGCGGGGTCGGACCGAACGCAGGCCACACCATAAACGTGCAAAATGAAAAGTACGTTG
TCCGCATGATTCCTTCAGGATTCCTCTATCCAAAAGCGGAACTCATGATCGGCAGCGGCGTACTGGTTGACCCGAGAGTA
TTTGCTCACGAACTTGACATCCTGAAGTGTGCCGGCAGGACCAGGATCGACAGCCGATGCGGAATCATCGAAGAAGAACA
CATCCTTCTCGACAAAAAAGACCCACACCTGTCAAAAACAGTCGGTTCAACCGGCTCAGGGTGCGGGCCTGCTAACTCAG
ACCGTGTCATGAGAAAAGGCAGACTTGCACGGGATGTCCCCGAACTCGCACCCTACATAACAGACGTCGCCCAAAACGTC
AACGACGCGATTGAAAAAGGCGACTCCATCCTTATCGAAGGAACGCAAGGGTTCGGCATCTCCCTTTACTATGGAAACTA
TCCCTTTGTCACCAGCAAAGACACTTCGGCATCCCAGATGGCTGCTGATGTCGGTGTCGGACCCACCAACATCGATGACG
TCGTCGTTGTCTTCAAAGCGTTTCCAACACGAGTCGGCGAAGGACCCTTCCCAACCGAACTTACCCATGACGAATCCATC
GCCCTTGGAATCCAGGAATTCGGCGCCGTTACCCACAGAGAACGCCGTATTGGTATGTGGGATGGAAAAATGGCCCGCTA
TTCTGCCATGATCAACGGCTGCACGACGATCGCAATAACCGGCATCGACCACATCGACGAATCGGTCTACGGAGCAACCG
ACTACGCAAAACTCACCCCGAAAGCACTGGCGTTCATCGACCAGATCGAAGCCGACACGGGAAAACCAGTAGGCATCATT
TCCACCGGCCCGGACCAGTCGCATATCATCGACATCAGATCAGAGCTCTAA

Upstream 100 bases:

>100_bases
GCAAGCTTTCAGCTTGCTCTGCTGAGGTCGGCCGCTTTCAGCGTTCAACCCGCTAACCCAAGTCCTTATCACCAACTGCA
TCCAATAATATTCGATAACC

Downstream 100 bases:

>100_bases
TTCATGAAAAGACGGATACTTGACATCATCTGCTGCCCCGTATGCAAGGGAAAGTTTATGCTTACAGAGATGGAGGGTAA
TGATACAGAAATAATGGAAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPSTIIVGGFFGDEGKGKIVAHIAQKDQATIIARGGVGPNAGHTINVQNEKYVVRMIPSGFLYPKAELMIGSGVLVDPRV
FAHELDILKCAGRTRIDSRCGIIEEEHILLDKKDPHLSKTVGSTGSGCGPANSDRVMRKGRLARDVPELAPYITDVAQNV
NDAIEKGDSILIEGTQGFGISLYYGNYPFVTSKDTSASQMAADVGVGPTNIDDVVVVFKAFPTRVGEGPFPTELTHDESI
ALGIQEFGAVTHRERRIGMWDGKMARYSAMINGCTTIAITGIDHIDESVYGATDYAKLTPKALAFIDQIEADTGKPVGII
STGPDQSHIIDIRSEL

Sequences:

>Translated_336_residues
MPSTIIVGGFFGDEGKGKIVAHIAQKDQATIIARGGVGPNAGHTINVQNEKYVVRMIPSGFLYPKAELMIGSGVLVDPRV
FAHELDILKCAGRTRIDSRCGIIEEEHILLDKKDPHLSKTVGSTGSGCGPANSDRVMRKGRLARDVPELAPYITDVAQNV
NDAIEKGDSILIEGTQGFGISLYYGNYPFVTSKDTSASQMAADVGVGPTNIDDVVVVFKAFPTRVGEGPFPTELTHDESI
ALGIQEFGAVTHRERRIGMWDGKMARYSAMINGCTTIAITGIDHIDESVYGATDYAKLTPKALAFIDQIEADTGKPVGII
STGPDQSHIIDIRSEL
>Mature_335_residues
PSTIIVGGFFGDEGKGKIVAHIAQKDQATIIARGGVGPNAGHTINVQNEKYVVRMIPSGFLYPKAELMIGSGVLVDPRVF
AHELDILKCAGRTRIDSRCGIIEEEHILLDKKDPHLSKTVGSTGSGCGPANSDRVMRKGRLARDVPELAPYITDVAQNVN
DAIEKGDSILIEGTQGFGISLYYGNYPFVTSKDTSASQMAADVGVGPTNIDDVVVVFKAFPTRVGEGPFPTELTHDESIA
LGIQEFGAVTHRERRIGMWDGKMARYSAMINGCTTIAITGIDHIDESVYGATDYAKLTPKALAFIDQIEADTGKPVGIIS
TGPDQSHIIDIRSEL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=339, Percent_Identity=30.6784660766962, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI22748717, Length=337, Percent_Identity=30.5637982195846, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI40316944, Length=303, Percent_Identity=29.042904290429, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=30.0469483568075, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI25146553, Length=336, Percent_Identity=31.547619047619, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146555, Length=336, Percent_Identity=31.547619047619, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324109, Length=342, Percent_Identity=32.7485380116959, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI21356033, Length=332, Percent_Identity=29.5180722891566, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 36027; Mature: 35896

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTIIVGGFFGDEGKGKIVAHIAQKDQATIIARGGVGPNAGHTINVQNEKYVVRMIPSG
CCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEECCCEEEEEECCCC
FLYPKAELMIGSGVLVDPRVFAHELDILKCAGRTRIDSRCGIIEEEHILLDKKDPHLSKT
CCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEECCCCCCHHHH
VGSTGSGCGPANSDRVMRKGRLARDVPELAPYITDVAQNVNDAIEKGDSILIEGTQGFGI
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEE
SLYYGNYPFVTSKDTSASQMAADVGVGPTNIDDVVVVFKAFPTRVGEGPFPTELTHDESI
EEEECCCCEEECCCCCHHHHHHHCCCCCCCCCCEEEEEEHHCHHCCCCCCCCCCCCCCCE
ALGIQEFGAVTHRERRIGMWDGKMARYSAMINGCTTIAITGIDHIDESVYGATDYAKLTP
EECHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCHHHCCH
KALAFIDQIEADTGKPVGIISTGPDQSHIIDIRSEL
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure 
PSTIIVGGFFGDEGKGKIVAHIAQKDQATIIARGGVGPNAGHTINVQNEKYVVRMIPSG
CCEEEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEECCCEEEEEECCCC
FLYPKAELMIGSGVLVDPRVFAHELDILKCAGRTRIDSRCGIIEEEHILLDKKDPHLSKT
CCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEECCCCCCHHHH
VGSTGSGCGPANSDRVMRKGRLARDVPELAPYITDVAQNVNDAIEKGDSILIEGTQGFGI
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEE
SLYYGNYPFVTSKDTSASQMAADVGVGPTNIDDVVVVFKAFPTRVGEGPFPTELTHDESI
EEEECCCCEEECCCCCHHHHHHHCCCCCCCCCCEEEEEEHHCHHCCCCCCCCCCCCCCCE
ALGIQEFGAVTHRERRIGMWDGKMARYSAMINGCTTIAITGIDHIDESVYGATDYAKLTP
EECHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCHHHCCH
KALAFIDQIEADTGKPVGIISTGPDQSHIIDIRSEL
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]