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The map label for this gene is frhA [H]

Identifier: 124484844

GI number: 124484844

Start: 14411

End: 15778

Strand: Reverse

Name: frhA [H]

Synonym: Mlab_0015

Alternate gene names: 124484844

Gene position: 15778-14411 (Counterclockwise)

Preceding gene: 124484845

Following gene: 124484843

Centisome position: 0.87

GC content: 52.92

Gene sequence:

>1368_bases
TTGTCGAAAGTAGTAGAAATTTCCCCGACCACCAGACATGAAGGTCACTCCAAGCTCGTTTTAAAGGTAAACGATGAAGG
CATCATCGAGCGCGGAGACTGGCTTTCTATCACACCGGTTCGTGGAGTAGAGAAACTTGCCATTGGTAAAACCTATGAGC
AGGTCCCAAAGATCGCTTCCCGTGTGTGCGGTATCTGTCCGATTGCCCACACGCTTGCCGCAACTGAGGCAATGGAAGCA
TCGATGGGTGTTGAGATCCCAGACGACGCATACCTCCTGCGTATGATTTTACAGTGCGGTAACAGACTGCACTCTCATGC
ACTGCACGACATTCTTGCACTGCCTGACTTATATCTCCCAGGAACCGACATCAAGGTCAACCCCTTCAGCCCAGAAGAGC
CGGTGAGATCTGTCGCAAAGCGTATTCAGACCCTTCGTATGATCGGTCAGACCGTCGGCGAGATCGTCGGCGGCGAAGCA
GTACACCCGTCCAACCCCCGTGTTGGAGGTATGTACAAAAACATTACTCCCCGTGCAAAAGCTAAGATCTACGATCTTGC
AAAGCAGGGACTCCAGCTTGCACGTGAACAGATGGAGTTCATGATTGCTCTGGAAGAGAACTACATGAAACGTGACTGGG
CAGAAATGTGCGGCCGCGAAGTTGCCATTCCAAAAGAACTTGGTTACCACAACCAGGGCTACATGGCAGCAGCTCCGATG
TACGGCAGCTCTTCCCTTGACGAGAACCCCGAGTGGGATCCTGCACGCTGGCTTGAAGTCAGACCATGGGACTGGTATAT
GGGCGAAGAAGAGATCACTCTCGAAGACCCTACATACCCGGTCGGCGGAACCACCAAAGCAGGCACGAAAGCTTGGCCGC
AGATGCAGGCATGCACCGGCGTACCTCTGTACGACGGACAGCCTGTTGAGGTTGGTCCCCGTGCACGTCTTGTAAAATAC
CAGAACTACAACCGCAAAGGTGCAATGGGATTAAACATTGCCCGTGCGATGGAGTATATGGACTGTATGTACAAGATGAT
TGAGGCAGTTGATGCAATTGACACCAGCGCCTCAGTTTGTGCAGATGTTATCCCTCAGGGTGACGGCGAAATCGGATGGG
CAGCAAACGAAGCACCGCGTGGCTGTGATGTGCACCTTGCAAAGGTACGCAACGGCAAAGTCGAATGGTTCTCCATGCTT
GTCCCGACCACCTGGAACTTCCCGGTCTGCAGCCGTGCACTTACCGGCGCACCCTGGGAACTTGCAGAAGTTATCGTCCG
TGCATACGACCCCTGTGTTTCCTGCGCAACCCACATGATTGTGCTGGATGAAAACAACAAGCTCGTTGCACAGAAGCTCC
TTGAGTAA

Upstream 100 bases:

>100_bases
ATTAGCGTACTTCTGTACGTGTCATTAATCCAAAAGGACAATGGTAATATTCCTGTATAGCTGTTTGAAAACCTAAATAA
TTTAGGATGAGGCGAAAACA

Downstream 100 bases:

>100_bases
GTATATACCATGGATGGACTGTTTCCAGAAATAATAATCGCCGGTGTCGGTAACGAACTCTTTGGCGACGACGGATTTGG
TCCCGTTGTGATCAGAGCTT

Product: coenzyme F420-reducing hydrogenase, alpha subunit

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha; FRH [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSKVVEISPTTRHEGHSKLVLKVNDEGIIERGDWLSITPVRGVEKLAIGKTYEQVPKIASRVCGICPIAHTLAATEAMEA
SMGVEIPDDAYLLRMILQCGNRLHSHALHDILALPDLYLPGTDIKVNPFSPEEPVRSVAKRIQTLRMIGQTVGEIVGGEA
VHPSNPRVGGMYKNITPRAKAKIYDLAKQGLQLAREQMEFMIALEENYMKRDWAEMCGREVAIPKELGYHNQGYMAAAPM
YGSSSLDENPEWDPARWLEVRPWDWYMGEEEITLEDPTYPVGGTTKAGTKAWPQMQACTGVPLYDGQPVEVGPRARLVKY
QNYNRKGAMGLNIARAMEYMDCMYKMIEAVDAIDTSASVCADVIPQGDGEIGWAANEAPRGCDVHLAKVRNGKVEWFSML
VPTTWNFPVCSRALTGAPWELAEVIVRAYDPCVSCATHMIVLDENNKLVAQKLLE

Sequences:

>Translated_455_residues
MSKVVEISPTTRHEGHSKLVLKVNDEGIIERGDWLSITPVRGVEKLAIGKTYEQVPKIASRVCGICPIAHTLAATEAMEA
SMGVEIPDDAYLLRMILQCGNRLHSHALHDILALPDLYLPGTDIKVNPFSPEEPVRSVAKRIQTLRMIGQTVGEIVGGEA
VHPSNPRVGGMYKNITPRAKAKIYDLAKQGLQLAREQMEFMIALEENYMKRDWAEMCGREVAIPKELGYHNQGYMAAAPM
YGSSSLDENPEWDPARWLEVRPWDWYMGEEEITLEDPTYPVGGTTKAGTKAWPQMQACTGVPLYDGQPVEVGPRARLVKY
QNYNRKGAMGLNIARAMEYMDCMYKMIEAVDAIDTSASVCADVIPQGDGEIGWAANEAPRGCDVHLAKVRNGKVEWFSML
VPTTWNFPVCSRALTGAPWELAEVIVRAYDPCVSCATHMIVLDENNKLVAQKLLE
>Mature_454_residues
SKVVEISPTTRHEGHSKLVLKVNDEGIIERGDWLSITPVRGVEKLAIGKTYEQVPKIASRVCGICPIAHTLAATEAMEAS
MGVEIPDDAYLLRMILQCGNRLHSHALHDILALPDLYLPGTDIKVNPFSPEEPVRSVAKRIQTLRMIGQTVGEIVGGEAV
HPSNPRVGGMYKNITPRAKAKIYDLAKQGLQLAREQMEFMIALEENYMKRDWAEMCGREVAIPKELGYHNQGYMAAAPMY
GSSSLDENPEWDPARWLEVRPWDWYMGEEEITLEDPTYPVGGTTKAGTKAWPQMQACTGVPLYDGQPVEVGPRARLVKYQ
NYNRKGAMGLNIARAMEYMDCMYKMIEAVDAIDTSASVCADVIPQGDGEIGWAANEAPRGCDVHLAKVRNGKVEWFSMLV
PTTWNFPVCSRALTGAPWELAEVIVRAYDPCVSCATHMIVLDENNKLVAQKLLE

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789076, Length=206, Percent_Identity=29.126213592233, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788832, Length=225, Percent_Identity=26.2222222222222, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789368, Length=109, Percent_Identity=29.3577981651376, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787207, Length=103, Percent_Identity=31.0679611650485, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017682
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 50516; Mature: 50385

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVVEISPTTRHEGHSKLVLKVNDEGIIERGDWLSITPVRGVEKLAIGKTYEQVPKIAS
CCCEEECCCCCCCCCCCEEEEEECCCCCEECCCEEEECCCCCHHHHHHCCHHHHHHHHHH
RVCGICPIAHTLAATEAMEASMGVEIPDDAYLLRMILQCGNRLHSHALHDILALPDLYLP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTDIKVNPFSPEEPVRSVAKRIQTLRMIGQTVGEIVGGEAVHPSNPRVGGMYKNITPRAK
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCHHH
AKIYDLAKQGLQLAREQMEFMIALEENYMKRDWAEMCGREVAIPKELGYHNQGYMAAAPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEECCC
YGSSSLDENPEWDPARWLEVRPWDWYMGEEEITLEDPTYPVGGTTKAGTKAWPQMQACTG
CCCCCCCCCCCCCCCCEEEECCCCEECCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCC
VPLYDGQPVEVGPRARLVKYQNYNRKGAMGLNIARAMEYMDCMYKMIEAVDAIDTSASVC
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ADVIPQGDGEIGWAANEAPRGCDVHLAKVRNGKVEWFSMLVPTTWNFPVCSRALTGAPWE
HHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHCCCCHH
LAEVIVRAYDPCVSCATHMIVLDENNKLVAQKLLE
HHHHHHHHHCHHHHHHEEEEEEECCCHHHHHHHCC
>Mature Secondary Structure 
SKVVEISPTTRHEGHSKLVLKVNDEGIIERGDWLSITPVRGVEKLAIGKTYEQVPKIAS
CCEEECCCCCCCCCCCEEEEEECCCCCEECCCEEEECCCCCHHHHHHCCHHHHHHHHHH
RVCGICPIAHTLAATEAMEASMGVEIPDDAYLLRMILQCGNRLHSHALHDILALPDLYLP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTDIKVNPFSPEEPVRSVAKRIQTLRMIGQTVGEIVGGEAVHPSNPRVGGMYKNITPRAK
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCHHH
AKIYDLAKQGLQLAREQMEFMIALEENYMKRDWAEMCGREVAIPKELGYHNQGYMAAAPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEECCC
YGSSSLDENPEWDPARWLEVRPWDWYMGEEEITLEDPTYPVGGTTKAGTKAWPQMQACTG
CCCCCCCCCCCCCCCCEEEECCCCEECCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCC
VPLYDGQPVEVGPRARLVKYQNYNRKGAMGLNIARAMEYMDCMYKMIEAVDAIDTSASVC
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ADVIPQGDGEIGWAANEAPRGCDVHLAKVRNGKVEWFSMLVPTTWNFPVCSRALTGAPWE
HHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHCCCCHH
LAEVIVRAYDPCVSCATHMIVLDENNKLVAQKLLE
HHHHHHHHHCHHHHHHEEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8521835 [H]