BacMap

Definition	Methylibium petroleiphilum PM1 chromosome, complete genome.
Accession	NC_008825
Length	4,044,195

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The map label for this gene is linX [H]

Identifier: 124266076

GI number: 124266076

Start: 924953

End: 925801

Strand: Direct

Name: linX [H]

Synonym: Mpe_A0883

Alternate gene names: 124266076

Gene position: 924953-925801 (Clockwise)

Preceding gene: 124266075

Following gene: 124266077

Centisome position: 22.87

GC content: 70.79

Gene sequence:

>849_bases
ATGACCGACAGCCGCGCCAGCACCCCGGCTCCCATCGACTACGGCAAGGCCTACCGCGTGGACGGCAAGGTCGCGCTGAT
CAGCGGCGCGGCGCGCGGCCTCGGCGCCGAGATGGCAAGGGCGCTGGCGGGTGCCGGTGCGCGCGTGATGGTCACCGACG
TGCTCGAGTCGCAGGGTCGCGAGACGGTGGCCGAGATCCGCGCCGCCGGCGGCCAGGCCGACTTCTTCCGGCTCGACACC
ACCGACGAGGCGCAGTGGGAGGCCGCGATCGCCGTCACGATCGAGCGGCTCGGCGGCCTGCATGTCCTCGTCAACAACGC
GGGTATCGAGCGCCTGCAGTTCATCACCGAGACCACGGTCGAGGAGTTCCGCAAGGTGCAGGACGTCAACGTGACCGGCG
TGTTCCTCGGCTGCAAGCACGCGATCCGCGCGATGCGGCCGGGCGGTGCGGCCGGGCAGGGCGGCTCGATCATCAACCTG
TCGTCGGTCGCCGGCCTGGTGGGCTGCATCGGACTGAGCAACTACTGCGCGTCCAAGGGCGCGGTGCGGCTGCTGACGAA
GTCGGTCGCCGTCGAGTGCGGGCAACTCAAGCTCGGCATCCGCTGCAACTCCATCCACCCCGGCGTGGTGTGGACCGCGA
TGGGGCGCGATCTGTTCCAGCACTTCGTCGACCTGAAGATCATGCCCGACCTGGCGGCGGCGCAGACCGCCTTCGAGACG
GCGATCCCGATGGGCCGCTGCGGCGCGCCCGAGGACGTGGCCTCGGCTGCGCTCTACCTCGCCTCCGATGCCGCGAAGTG
GGTGACCGGCGCCGAGTTCGTGGTCGACGGCGGCTACACCGCGGTCTGA

Upstream 100 bases:

>100_bases
CAATCATCCGGCCTCGCCATGTGCTCGCGCTGCGCCGCGAGTCGCGCCCATGCCTCCGGTCGGGCCGATGCCCGCACCGA
CAAGAACCAGGAGACCCCCC

Downstream 100 bases:

>100_bases
CGGCGGCGCAGCGCACACGACAACGGACAGGAGATCAGGACATGGGACGTGTGGACAACAAGGTGGCGCTGGTGACCGGT
GGTGCCAAGGGCATCGGCCG

Product: putative dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTDSRASTPAPIDYGKAYRVDGKVALISGAARGLGAEMARALAGAGARVMVTDVLESQGRETVAEIRAAGGQADFFRLDT
TDEAQWEAAIAVTIERLGGLHVLVNNAGIERLQFITETTVEEFRKVQDVNVTGVFLGCKHAIRAMRPGGAAGQGGSIINL
SSVAGLVGCIGLSNYCASKGAVRLLTKSVAVECGQLKLGIRCNSIHPGVVWTAMGRDLFQHFVDLKIMPDLAAAQTAFET
AIPMGRCGAPEDVASAALYLASDAAKWVTGAEFVVDGGYTAV

Sequences:

>Translated_282_residues
MTDSRASTPAPIDYGKAYRVDGKVALISGAARGLGAEMARALAGAGARVMVTDVLESQGRETVAEIRAAGGQADFFRLDT
TDEAQWEAAIAVTIERLGGLHVLVNNAGIERLQFITETTVEEFRKVQDVNVTGVFLGCKHAIRAMRPGGAAGQGGSIINL
SSVAGLVGCIGLSNYCASKGAVRLLTKSVAVECGQLKLGIRCNSIHPGVVWTAMGRDLFQHFVDLKIMPDLAAAQTAFET
AIPMGRCGAPEDVASAALYLASDAAKWVTGAEFVVDGGYTAV
>Mature_281_residues
TDSRASTPAPIDYGKAYRVDGKVALISGAARGLGAEMARALAGAGARVMVTDVLESQGRETVAEIRAAGGQADFFRLDTT
DEAQWEAAIAVTIERLGGLHVLVNNAGIERLQFITETTVEEFRKVQDVNVTGVFLGCKHAIRAMRPGGAAGQGGSIINLS
SVAGLVGCIGLSNYCASKGAVRLLTKSVAVECGQLKLGIRCNSIHPGVVWTAMGRDLFQHFVDLKIMPDLAAAQTAFETA
IPMGRCGAPEDVASAALYLASDAAKWVTGAEFVVDGGYTAV

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ). LinX appears not to be involved in gamma-HCH degradation pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI59889578, Length=240, Percent_Identity=35.4166666666667, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI15277342, Length=271, Percent_Identity=33.9483394833948, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI40254992, Length=260, Percent_Identity=31.1538461538462, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI7705925, Length=260, Percent_Identity=31.5384615384615, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI66933014, Length=263, Percent_Identity=34.2205323193916, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI32483357, Length=258, Percent_Identity=30.6201550387597, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI31542939, Length=199, Percent_Identity=33.6683417085427, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI19923817, Length=271, Percent_Identity=27.3062730627306, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4504505, Length=228, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4758504, Length=247, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI109715829, Length=256, Percent_Identity=29.6875, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI224922801, Length=180, Percent_Identity=32.7777777777778, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI83715985, Length=191, Percent_Identity=33.5078534031414, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=27.4131274131274, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI40807363, Length=200, Percent_Identity=32.5, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI126723750, Length=263, Percent_Identity=27.3764258555133, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI20149619, Length=207, Percent_Identity=29.951690821256, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI142976729, Length=200, Percent_Identity=28, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7706318, Length=197, Percent_Identity=25.3807106598985, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI186928839, Length=271, Percent_Identity=29.520295202952, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI210032110, Length=199, Percent_Identity=27.6381909547739, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI5031737, Length=260, Percent_Identity=26.1538461538462, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI166795268, Length=251, Percent_Identity=28.6852589641434, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787335, Length=262, Percent_Identity=32.4427480916031, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=34.2205323193916, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787905, Length=260, Percent_Identity=33.4615384615385, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788459, Length=259, Percent_Identity=34.3629343629344, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1790717, Length=262, Percent_Identity=32.0610687022901, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI2367175, Length=267, Percent_Identity=35.9550561797753, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87082160, Length=262, Percent_Identity=32.824427480916, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789208, Length=276, Percent_Identity=31.5217391304348, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786812, Length=266, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789378, Length=283, Percent_Identity=28.2685512367491, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI2367365, Length=261, Percent_Identity=28.3524904214559, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787526, Length=266, Percent_Identity=27.8195488721804, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787820, Length=240, Percent_Identity=24.1666666666667, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=259, Percent_Identity=33.976833976834, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17555706, Length=265, Percent_Identity=31.6981132075472, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17562910, Length=279, Percent_Identity=29.7491039426523, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560332, Length=276, Percent_Identity=32.2463768115942, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17551412, Length=280, Percent_Identity=30.3571428571429, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538480, Length=280, Percent_Identity=30.7142857142857, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17559104, Length=278, Percent_Identity=29.136690647482, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI115534694, Length=263, Percent_Identity=31.1787072243346, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560150, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17564282, Length=196, Percent_Identity=31.6326530612245, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562906, Length=286, Percent_Identity=27.972027972028, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193203117, Length=196, Percent_Identity=30.1020408163265, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=269, Percent_Identity=29.368029739777, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17562904, Length=278, Percent_Identity=29.136690647482, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17538182, Length=265, Percent_Identity=32.4528301886792, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17508651, Length=271, Percent_Identity=32.8413284132841, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193203115, Length=195, Percent_Identity=30.2564102564103, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560676, Length=260, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=259, Percent_Identity=29.3436293436293, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17565030, Length=280, Percent_Identity=29.2857142857143, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17567345, Length=197, Percent_Identity=32.994923857868, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=262, Percent_Identity=28.2442748091603, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562908, Length=283, Percent_Identity=28.6219081272085, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17562990, Length=280, Percent_Identity=28.2142857142857, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538486, Length=280, Percent_Identity=27.5, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=280, Percent_Identity=27.8571428571429, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=278, Percent_Identity=29.4964028776978, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17508895, Length=193, Percent_Identity=29.0155440414508, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=273, Percent_Identity=28.9377289377289, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=274, Percent_Identity=27.3722627737226, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=199, Percent_Identity=33.6683417085427, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17561272, Length=272, Percent_Identity=30.1470588235294, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=275, Percent_Identity=28.7272727272727, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17563726, Length=274, Percent_Identity=26.6423357664234, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536025, Length=270, Percent_Identity=30, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71982365, Length=201, Percent_Identity=27.363184079602, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=262, Percent_Identity=25.9541984732824, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=194, Percent_Identity=38.1443298969072, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324126, Length=261, Percent_Identity=26.0536398467433, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571526, Length=268, Percent_Identity=32.089552238806, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI23397609, Length=264, Percent_Identity=34.469696969697, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24639444, Length=258, Percent_Identity=31.0077519379845, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640492, Length=224, Percent_Identity=35.7142857142857, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357041, Length=271, Percent_Identity=30.9963099630996, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355319, Length=268, Percent_Identity=29.4776119402985, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24643142, Length=261, Percent_Identity=32.183908045977, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24644339, Length=268, Percent_Identity=30.5970149253731, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364892, Length=204, Percent_Identity=32.3529411764706, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17737361, Length=244, Percent_Identity=30.327868852459, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI18859837, Length=265, Percent_Identity=30.9433962264151, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24644337, Length=207, Percent_Identity=32.3671497584541, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648944, Length=204, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364494, Length=228, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24663911, Length=257, Percent_Identity=30.3501945525292, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24665243, Length=258, Percent_Identity=25.968992248062, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221331218, Length=258, Percent_Identity=25.968992248062, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641232, Length=249, Percent_Identity=28.5140562248996, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28571387, Length=255, Percent_Identity=30.1960784313725, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24656154, Length=220, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19922628, Length=220, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24665247, Length=259, Percent_Identity=25.8687258687259, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221331216, Length=259, Percent_Identity=25.8687258687259, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 29402; Mature: 29271

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSRASTPAPIDYGKAYRVDGKVALISGAARGLGAEMARALAGAGARVMVTDVLESQGR
CCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHCCH
ETVAEIRAAGGQADFFRLDTTDEAQWEAAIAVTIERLGGLHVLVNNAGIERLQFITETTV
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHEEEHHHHCCEEEEEECCCHHHHHHHHHHHH
EEFRKVQDVNVTGVFLGCKHAIRAMRPGGAAGQGGSIINLSSVAGLVGCIGLSNYCASKG
HHHHHHHCCCEEEEEECHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCC
AVRLLTKSVAVECGQLKLGIRCNSIHPGVVWTAMGRDLFQHFVDLKIMPDLAAAQTAFET
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHH
AIPMGRCGAPEDVASAALYLASDAAKWVTGAEFVVDGGYTAV
HCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCEEEECCCEECC
>Mature Secondary Structure 
TDSRASTPAPIDYGKAYRVDGKVALISGAARGLGAEMARALAGAGARVMVTDVLESQGR
CCCCCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHCCH
ETVAEIRAAGGQADFFRLDTTDEAQWEAAIAVTIERLGGLHVLVNNAGIERLQFITETTV
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHEEEHHHHCCEEEEEECCCHHHHHHHHHHHH
EEFRKVQDVNVTGVFLGCKHAIRAMRPGGAAGQGGSIINLSSVAGLVGCIGLSNYCASKG
HHHHHHHCCCEEEEEECHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCC
AVRLLTKSVAVECGQLKLGIRCNSIHPGVVWTAMGRDLFQHFVDLKIMPDLAAAQTAFET
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHH
AIPMGRCGAPEDVASAALYLASDAAKWVTGAEFVVDGGYTAV
HCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]