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The map label for this gene is miaB

Identifier: 124265725

GI number: 124265725

Start: 568381

End: 569754

Strand: Reverse

Name: miaB

Synonym: Mpe_A0532

Alternate gene names: 124265725

Gene position: 569754-568381 (Counterclockwise)

Preceding gene: 124265726

Following gene: 124265717

Centisome position: 14.09

GC content: 67.39

Gene sequence:

>1374_bases
ATGAGCGCCGCCGGCACCGGCAAGAAGGTCTTCATCAAGACCTTCGGTTGCCAGATGAACGAGTACGACTCGGACAAGAT
GGCCGACGTGCTGCGCGCGGCCGAGGGCTATGAGCCCACGACCGACGTGGAGCAGGCCGACCTGATCCTCTTCAACACCT
GCTCCGTGCGCGAGAAGGCACAGGAGAAGGTGTTCAGCGACCTGGGGCGCGTGAAACACCTGAAGGCCAGGGGCGTGAAG
ATCGGCGTGGGCGGCTGCGTTGCCAGCCAGGAAGGCGCGGCACTGATCGAGCGTGCGCCTTACGTCGACGTGGTGTTCGG
TCCGCAGACGCTGCACCGCCTGCCGGAAATGCTGGCTCGTCGCGATGCGCAGCAGCGCCCGCAGGTCGACATCAGCTTCC
CCGAGATCGAGAAGTTCGACCATCTGCCGCCGGCGCGCGTCGACGGCGCGACCGCCTTCGTCTCGATCATGGAAGGCTGC
TCCAAGTACTGCAGCTACTGCGTCGTGCCCTACACGCGCGGCGAGGAAGTGTCGCGGCCGTTCGACGACGTTCTCACCGA
AGTGGCCAGCCTGGCCGACCAGGGCGTCAAGGAAGTGACGCTGCTGGGCCAGAACGTCAACGGCTACCGCGGCCGCATGG
GCGACACGGCCGAGATCGCCGACTTCGCGCTGCTGCTGGAGTACGTGGCGGAGATCCCGGGCATCGCCCGCATCCGCTAC
ACCACCAGCCATCCGAACGAGTTCTCGCAGCGGCTGATCGACGTGTACGCCCGCGTGCCGCAGCTCGTGAACCACCTGCA
CCTGCCGGTGCAGCACGGCAGCGACCGCATCCTGAGCGCGATGAAGCGCGGCTACACGTCGCTGGAGTACAAGTCTTCGA
TCCGCAAGCTGCGCGCGATCCGGCCCGACATCAGCCTGTCGACCGACTTCATCGTCGGCTTCCCCGGCGAGACCGATGAC
GACCACGCGCGCACGATGAAGTTGATCGAGGACGTGGGCTTCGACCACAGCTTCAGCTTCATCTACAGCGCCCGCCCCGG
CACGCCGGCCGCGGCACTGCACGACGACGCGCCGCGCGAACTCAAGCTGGCCCGTTTGCAGCAGGTGCAGGAAGCGATCG
AGGCTAACGGCCGGCGCATCGGCCAGAGCCGCGTCGGCACCGTGCAGCGCATCCTCGTCGAAGGGCCGTCGCGCCGGCCT
GAGCTCAATGCCGGCCACGAGTTGATGGGCCGCACCGAGTGCAACCGCATCGTCAACTTCGCCGGTCCGGCCCGCCTGAC
CGGACAGCTGATCGACGTGACGATCACCGAGGCCTACCCGCACTCGCTGCGCGGCGAGGTGCTGCTGCGGGAGCGCAGCC
CGCAGCCGGCCTGA

Upstream 100 bases:

>100_bases
GGGTGCTGCGCGCCGAAGAGAAAACCGGCCTCTGGCCGAAGACCGCCGACGAGGCCGCGCGCTGGTCCTTCGACCCGGCC
GCGCAGGCCTGGACCAAGCC

Downstream 100 bases:

>100_bases
CGGCTGCTGCGCGGCCGCTCAGACGGCCGGCCCCAGCACCGCAAAGAAGTGGCAGGTGCTGCCGGCCAGCACGAACAGGT
GCCAGACGAAGTGCCCGAAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSAAGTGKKVFIKTFGCQMNEYDSDKMADVLRAAEGYEPTTDVEQADLILFNTCSVREKAQEKVFSDLGRVKHLKARGVK
IGVGGCVASQEGAALIERAPYVDVVFGPQTLHRLPEMLARRDAQQRPQVDISFPEIEKFDHLPPARVDGATAFVSIMEGC
SKYCSYCVVPYTRGEEVSRPFDDVLTEVASLADQGVKEVTLLGQNVNGYRGRMGDTAEIADFALLLEYVAEIPGIARIRY
TTSHPNEFSQRLIDVYARVPQLVNHLHLPVQHGSDRILSAMKRGYTSLEYKSSIRKLRAIRPDISLSTDFIVGFPGETDD
DHARTMKLIEDVGFDHSFSFIYSARPGTPAAALHDDAPRELKLARLQQVQEAIEANGRRIGQSRVGTVQRILVEGPSRRP
ELNAGHELMGRTECNRIVNFAGPARLTGQLIDVTITEAYPHSLRGEVLLRERSPQPA

Sequences:

>Translated_457_residues
MSAAGTGKKVFIKTFGCQMNEYDSDKMADVLRAAEGYEPTTDVEQADLILFNTCSVREKAQEKVFSDLGRVKHLKARGVK
IGVGGCVASQEGAALIERAPYVDVVFGPQTLHRLPEMLARRDAQQRPQVDISFPEIEKFDHLPPARVDGATAFVSIMEGC
SKYCSYCVVPYTRGEEVSRPFDDVLTEVASLADQGVKEVTLLGQNVNGYRGRMGDTAEIADFALLLEYVAEIPGIARIRY
TTSHPNEFSQRLIDVYARVPQLVNHLHLPVQHGSDRILSAMKRGYTSLEYKSSIRKLRAIRPDISLSTDFIVGFPGETDD
DHARTMKLIEDVGFDHSFSFIYSARPGTPAAALHDDAPRELKLARLQQVQEAIEANGRRIGQSRVGTVQRILVEGPSRRP
ELNAGHELMGRTECNRIVNFAGPARLTGQLIDVTITEAYPHSLRGEVLLRERSPQPA
>Mature_456_residues
SAAGTGKKVFIKTFGCQMNEYDSDKMADVLRAAEGYEPTTDVEQADLILFNTCSVREKAQEKVFSDLGRVKHLKARGVKI
GVGGCVASQEGAALIERAPYVDVVFGPQTLHRLPEMLARRDAQQRPQVDISFPEIEKFDHLPPARVDGATAFVSIMEGCS
KYCSYCVVPYTRGEEVSRPFDDVLTEVASLADQGVKEVTLLGQNVNGYRGRMGDTAEIADFALLLEYVAEIPGIARIRYT
TSHPNEFSQRLIDVYARVPQLVNHLHLPVQHGSDRILSAMKRGYTSLEYKSSIRKLRAIRPDISLSTDFIVGFPGETDDD
HARTMKLIEDVGFDHSFSFIYSARPGTPAAALHDDAPRELKLARLQQVQEAIEANGRRIGQSRVGTVQRILVEGPSRRPE
LNAGHELMGRTECNRIVNFAGPARLTGQLIDVTITEAYPHSLRGEVLLRERSPQPA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=495, Percent_Identity=36.969696969697, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI28872782, Length=495, Percent_Identity=36.969696969697, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=28.0087527352298, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=61.8834080717489, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1787057, Length=433, Percent_Identity=28.8683602771363, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=34.1825902335456, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=259, Percent_Identity=30.8880308880309, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356207, Length=443, Percent_Identity=34.7629796839729, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI19922432, Length=455, Percent_Identity=29.010989010989, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_METPP (A2SD55)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019729.1
- ProteinModelPortal:   A2SD55
- SMR:   A2SD55
- STRING:   A2SD55
- GeneID:   4787355
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0532
- NMPDR:   fig|279263.3.peg.2776
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   A2SD55
- ProtClustDB:   PRK14325
- BioCyc:   MPET420662:MPE_A0532-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50623; Mature: 50492

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAGTGKKVFIKTFGCQMNEYDSDKMADVLRAAEGYEPTTDVEQADLILFNTCSVREKA
CCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHH
QEKVFSDLGRVKHLKARGVKIGVGGCVASQEGAALIERAPYVDVVFGPQTLHRLPEMLAR
HHHHHHHHHHHHHHHHCCEEEECCCEECCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHH
RDAQQRPQVDISFPEIEKFDHLPPARVDGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRP
CCHHHCCCEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCCHHCCC
FDDVLTEVASLADQGVKEVTLLGQNVNGYRGRMGDTAEIADFALLLEYVAEIPGIARIRY
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
TTSHPNEFSQRLIDVYARVPQLVNHLHLPVQHGSDRILSAMKRGYTSLEYKSSIRKLRAI
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
RPDISLSTDFIVGFPGETDDDHARTMKLIEDVGFDHSFSFIYSARPGTPAAALHDDAPRE
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHCCCCCCHH
LKLARLQQVQEAIEANGRRIGQSRVGTVQRILVEGPSRRPELNAGHELMGRTECNRIVNF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
AGPARLTGQLIDVTITEAYPHSLRGEVLLRERSPQPA
CCCHHHCCEEEEEEEECCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
SAAGTGKKVFIKTFGCQMNEYDSDKMADVLRAAEGYEPTTDVEQADLILFNTCSVREKA
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHH
QEKVFSDLGRVKHLKARGVKIGVGGCVASQEGAALIERAPYVDVVFGPQTLHRLPEMLAR
HHHHHHHHHHHHHHHHCCEEEECCCEECCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHH
RDAQQRPQVDISFPEIEKFDHLPPARVDGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRP
CCHHHCCCEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCCHHCCC
FDDVLTEVASLADQGVKEVTLLGQNVNGYRGRMGDTAEIADFALLLEYVAEIPGIARIRY
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
TTSHPNEFSQRLIDVYARVPQLVNHLHLPVQHGSDRILSAMKRGYTSLEYKSSIRKLRAI
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
RPDISLSTDFIVGFPGETDDDHARTMKLIEDVGFDHSFSFIYSARPGTPAAALHDDAPRE
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHCCCCCCHH
LKLARLQQVQEAIEANGRRIGQSRVGTVQRILVEGPSRRPELNAGHELMGRTECNRIVNF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
AGPARLTGQLIDVTITEAYPHSLRGEVLLRERSPQPA
CCCHHHCCEEEEEEEECCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA