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The map label for this gene is fadA [H]

Identifier: 124265609

GI number: 124265609

Start: 448760

End: 449959

Strand: Reverse

Name: fadA [H]

Synonym: Mpe_A0416

Alternate gene names: 124265609

Gene position: 449959-448760 (Counterclockwise)

Preceding gene: 124265610

Following gene: 124265608

Centisome position: 11.13

GC content: 71.0

Gene sequence:

>1200_bases
ATGGCTAAGCAAGTTCAAGACGCCTACATCGTCGCCGCCACCCGCACCCCGATCGGCAAGTCCGGGCGGGGCTACTTCCG
CAACACGCGGCCCGACGATCTGCTGGTCGCGGCGGTGCAGAGTGCGCTGCGGCAGGTGCCCACGCTCGACCCGAAGGCGA
TCGAGGACGCCATCGTCGGCTGCTCCTTCCCCGAGGGCGAGCAGGGCATGAACATCGCGCGCGCCGCGATGCTGCTGGCC
GGCCTGCCGCAGTCGGTGGGCGGCGTCACGGTCAACCGCTTCTGCGCCTCGGGCCTGACCGCGCTGCAGATGGCCGCCGA
CCGCATCCGCATCGGCGAGGCCGACGTGATGATCGCCGGCGGCGCCGAGTCGATGAGCCTGGTGCCGATGGGCGGCAACA
AGCCCTCGTTCAACCCCGCCGTGTTCGAGAAGGACGAGAACGTGGGCATCGCCTACGGCATGGGCCTCACCGCCGAGAAG
GTCGCGGCGCAGTGGAAGGTGAGCCGCGAGGCGCAGGACGCCTTCGCGCTGCAGTCGCACCAGCGGGCGCTCGCGGCCCA
GGCGGCCGGCGAGTTCACCGACGAGATGACGCCCATCGACGTGGTCGACCGCTTTCCGAATCTGGCCACCGGCGAGGTCG
GCAGCAAGACCCGTACGGTCACCCTCGACGAAGGCCCGCGCCCCGACACCTCGCTGGAAGGCCTGGCCCGGCTGCGCCCG
GTGTTCGCCGCCAAGGGGTCGGTGACCGCGGGCAACAGCTCGCAGACCAGCGACGGCGCCGGCGCGCTGATCCTCGCCAG
CGAGAAGGCGGTGCGGCAGTTCGACCTGAAGCCGCTGGCCCGCTTCGTCAGCTATGCGATCCGCGGCGTGCCGCCCGAGA
TCATGGGCATCGGTCCGATCGAGGCGATCCCGCTGGCGTTGAAGCACGCCGGGCTGAAGCTCGACGACCTGGGCTGGATC
GAGCTGAACGAGGCCTTCGCGGCCCAGGCGCTGGCGGTCATCGGAAGCGTCGGGCTCGACCCGGCCAAGGTCAACCCGAT
GGGCGGCGCCATCGCCCTCGGCCACCCGCTGGGCGCCACCGGCGCCATCCGCTCGGCCACCGTCGTGCACGCGCTGCAAC
GCCACAACCTGAAGTACGGCATGGTGACGATGTGCGTCGGCATGGGCCAGGGGGCGGCGGGCATCCTTGAACGCGTCTGA

Upstream 100 bases:

>100_bases
ACCAACGGACTCCCTCTCCCGCTTGCGGGATAGGGGGCAGGACATCACCCCGACTCTCTTCCGCCCGCGGGAGAGCGAGT
GAACATCAAGGAGTCTTCAA

Downstream 100 bases:

>100_bases
CGCGCGGTGAACGCGATTCCCCTGACCTCGGGCGTCCTGCCACGCCGGCGCGTCCTGGTGGCCGGCCTCGCAAGCCTCGC
GACCGGCGGGTTGGCCGGCT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAKQVQDAYIVAATRTPIGKSGRGYFRNTRPDDLLVAAVQSALRQVPTLDPKAIEDAIVGCSFPEGEQGMNIARAAMLLA
GLPQSVGGVTVNRFCASGLTALQMAADRIRIGEADVMIAGGAESMSLVPMGGNKPSFNPAVFEKDENVGIAYGMGLTAEK
VAAQWKVSREAQDAFALQSHQRALAAQAAGEFTDEMTPIDVVDRFPNLATGEVGSKTRTVTLDEGPRPDTSLEGLARLRP
VFAAKGSVTAGNSSQTSDGAGALILASEKAVRQFDLKPLARFVSYAIRGVPPEIMGIGPIEAIPLALKHAGLKLDDLGWI
ELNEAFAAQALAVIGSVGLDPAKVNPMGGAIALGHPLGATGAIRSATVVHALQRHNLKYGMVTMCVGMGQGAAGILERV

Sequences:

>Translated_399_residues
MAKQVQDAYIVAATRTPIGKSGRGYFRNTRPDDLLVAAVQSALRQVPTLDPKAIEDAIVGCSFPEGEQGMNIARAAMLLA
GLPQSVGGVTVNRFCASGLTALQMAADRIRIGEADVMIAGGAESMSLVPMGGNKPSFNPAVFEKDENVGIAYGMGLTAEK
VAAQWKVSREAQDAFALQSHQRALAAQAAGEFTDEMTPIDVVDRFPNLATGEVGSKTRTVTLDEGPRPDTSLEGLARLRP
VFAAKGSVTAGNSSQTSDGAGALILASEKAVRQFDLKPLARFVSYAIRGVPPEIMGIGPIEAIPLALKHAGLKLDDLGWI
ELNEAFAAQALAVIGSVGLDPAKVNPMGGAIALGHPLGATGAIRSATVVHALQRHNLKYGMVTMCVGMGQGAAGILERV
>Mature_398_residues
AKQVQDAYIVAATRTPIGKSGRGYFRNTRPDDLLVAAVQSALRQVPTLDPKAIEDAIVGCSFPEGEQGMNIARAAMLLAG
LPQSVGGVTVNRFCASGLTALQMAADRIRIGEADVMIAGGAESMSLVPMGGNKPSFNPAVFEKDENVGIAYGMGLTAEKV
AAQWKVSREAQDAFALQSHQRALAAQAAGEFTDEMTPIDVVDRFPNLATGEVGSKTRTVTLDEGPRPDTSLEGLARLRPV
FAAKGSVTAGNSSQTSDGAGALILASEKAVRQFDLKPLARFVSYAIRGVPPEIMGIGPIEAIPLALKHAGLKLDDLGWIE
LNEAFAAQALAVIGSVGLDPAKVNPMGGAIALGHPLGATGAIRSATVVHALQRHNLKYGMVTMCVGMGQGAAGILERV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=45.0381679389313, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI167614485, Length=399, Percent_Identity=40.8521303258145, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=38.25, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI4557237, Length=406, Percent_Identity=36.2068965517241, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI4504327, Length=443, Percent_Identity=32.7313769751693, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=32.0610687022901, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1787663, Length=411, Percent_Identity=45.2554744525547, Blast_Score=310, Evalue=9e-86,
Organism=Escherichia coli, GI48994986, Length=400, Percent_Identity=45.75, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1788554, Length=406, Percent_Identity=41.1330049261084, Blast_Score=264, Evalue=6e-72,
Organism=Escherichia coli, GI87082165, Length=404, Percent_Identity=40.5940594059406, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788683, Length=441, Percent_Identity=32.4263038548753, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=42.3940149625935, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=33.5820895522388, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17535921, Length=407, Percent_Identity=32.9238329238329, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=444, Percent_Identity=30.8558558558559, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=395, Percent_Identity=30.8860759493671, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322031, Length=407, Percent_Identity=40.7862407862408, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6325229, Length=413, Percent_Identity=36.319612590799, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24655093, Length=408, Percent_Identity=38.7254901960784, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI17648125, Length=410, Percent_Identity=39.2682926829268, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24640423, Length=409, Percent_Identity=33.7408312958435, Blast_Score=198, Evalue=7e-51,
Organism=Drosophila melanogaster, GI17137578, Length=449, Percent_Identity=33.184855233853, Blast_Score=161, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 41520; Mature: 41389

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQVQDAYIVAATRTPIGKSGRGYFRNTRPDDLLVAAVQSALRQVPTLDPKAIEDAIVG
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
CSFPEGEQGMNIARAAMLLAGLPQSVGGVTVNRFCASGLTALQMAADRIRIGEADVMIAG
CCCCCCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEC
GAESMSLVPMGGNKPSFNPAVFEKDENVGIAYGMGLTAEKVAAQWKVSREAQDAFALQSH
CCCCEEEEECCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
QRALAAQAAGEFTDEMTPIDVVDRFPNLATGEVGSKTRTVTLDEGPRPDTSLEGLARLRP
HHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
VFAAKGSVTAGNSSQTSDGAGALILASEKAVRQFDLKPLARFVSYAIRGVPPEIMGIGPI
HHHCCCCEECCCCCCCCCCCCEEEEECCHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCCH
EAIPLALKHAGLKLDDLGWIELNEAFAAQALAVIGSVGLDPAKVNPMGGAIALGHPLGAT
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCC
GAIRSATVVHALQRHNLKYGMVTMCVGMGQGAAGILERV
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
AKQVQDAYIVAATRTPIGKSGRGYFRNTRPDDLLVAAVQSALRQVPTLDPKAIEDAIVG
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
CSFPEGEQGMNIARAAMLLAGLPQSVGGVTVNRFCASGLTALQMAADRIRIGEADVMIAG
CCCCCCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEC
GAESMSLVPMGGNKPSFNPAVFEKDENVGIAYGMGLTAEKVAAQWKVSREAQDAFALQSH
CCCCEEEEECCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
QRALAAQAAGEFTDEMTPIDVVDRFPNLATGEVGSKTRTVTLDEGPRPDTSLEGLARLRP
HHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
VFAAKGSVTAGNSSQTSDGAGALILASEKAVRQFDLKPLARFVSYAIRGVPPEIMGIGPI
HHHCCCCEECCCCCCCCCCCCEEEEECCHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCCH
EAIPLALKHAGLKLDDLGWIELNEAFAAQALAVIGSVGLDPAKVNPMGGAIALGHPLGAT
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCC
GAIRSATVVHALQRHNLKYGMVTMCVGMGQGAAGILERV
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]