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The map label for this gene is tag [H]

Identifier: 124265290

GI number: 124265290

Start: 91908

End: 92552

Strand: Reverse

Name: tag [H]

Synonym: Mpe_A0097

Alternate gene names: 124265290

Gene position: 92552-91908 (Counterclockwise)

Preceding gene: 124265291

Following gene: 124265287

Centisome position: 2.29

GC content: 69.77

Gene sequence:

>645_bases
ATGAGCCCACCGCTTCCCTCCGGCCTGTTCGACACCGCCACCGATGGCGCGCCACGCTGCACCTGGTGCGCGGCCACGCC
GGCCTACCAGCACTACCACGACCACGAATGGGGCTTCCCGGTCACCGACGACCGGCGGCTGTTCGAGAAACTCTGCCTCG
AAGGCTTCCAGGCCGGCCTGAGCTGGCTCACCATCCTGAACAAGCGCGAGAATTTCCGCGCCGCGTTCGCCGACTTCGAT
GCCGAGCGGCTGTCGCGCTACACCGGGCGCGATGTCGAGCGGCTGCTGGGCGACGCCGGCATCGTGCGCCACCGCGGCAA
GATCGAGTCGACTCTCAACAACGCCCGCCGTGTGATCGAGTTGCGCGCCGAGTTTGGTTCGCTGGCGGCCTACGCCTGGC
GCCATGAGCCGGCGCCCGCCTCGCGCCCCCGCCGCATGACGCTCGAGACACTGAAGACGCAGACCACCTCGCCCGAGTCG
GTGGCGATGAGCAAGGACCTGAAGCGGCGCGGCTGGAGCTTCGTCGGCCCGACCACGGTCTACGCGTTCATGCAGGCGAT
GGGGCTGGTCAACGACCACCTCGACGGCTGCCACGTGCGCGAACGAGCGCTGCAGGCGCGGCGCGGACTGCAGCCGCCGC
GCTGA

Upstream 100 bases:

>100_bases
GAAGACGGCGCGACGGTGGCACTGGCCGTCGCGGTGTGGTTGCACTGAAGATGCGGGGCGGCTCTCCGGCAGGCATGATG
CGCGCCGAGGAGTCCACCGC

Downstream 100 bases:

>100_bases
CGGCCGGGGCCCGGCCCGCTCAGCCGGGCGGCCGCAGGCGGTGCAGATCGGCCCGCGTGAAGAGCACGTCGCGATCCGAC
AACGCCTTGGCCAGCCGCTG

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSPPLPSGLFDTATDGAPRCTWCAATPAYQHYHDHEWGFPVTDDRRLFEKLCLEGFQAGLSWLTILNKRENFRAAFADFD
AERLSRYTGRDVERLLGDAGIVRHRGKIESTLNNARRVIELRAEFGSLAAYAWRHEPAPASRPRRMTLETLKTQTTSPES
VAMSKDLKRRGWSFVGPTTVYAFMQAMGLVNDHLDGCHVRERALQARRGLQPPR

Sequences:

>Translated_214_residues
MSPPLPSGLFDTATDGAPRCTWCAATPAYQHYHDHEWGFPVTDDRRLFEKLCLEGFQAGLSWLTILNKRENFRAAFADFD
AERLSRYTGRDVERLLGDAGIVRHRGKIESTLNNARRVIELRAEFGSLAAYAWRHEPAPASRPRRMTLETLKTQTTSPES
VAMSKDLKRRGWSFVGPTTVYAFMQAMGLVNDHLDGCHVRERALQARRGLQPPR
>Mature_213_residues
SPPLPSGLFDTATDGAPRCTWCAATPAYQHYHDHEWGFPVTDDRRLFEKLCLEGFQAGLSWLTILNKRENFRAAFADFDA
ERLSRYTGRDVERLLGDAGIVRHRGKIESTLNNARRVIELRAEFGSLAAYAWRHEPAPASRPRRMTLETLKTQTTSPESV
AMSKDLKRRGWSFVGPTTVYAFMQAMGLVNDHLDGCHVRERALQARRGLQPPR

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=179, Percent_Identity=47.4860335195531, Blast_Score=183, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 24287; Mature: 24156

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPLPSGLFDTATDGAPRCTWCAATPAYQHYHDHEWGFPVTDDRRLFEKLCLEGFQAGL
CCCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SWLTILNKRENFRAAFADFDAERLSRYTGRDVERLLGDAGIVRHRGKIESTLNNARRVIE
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAEFGSLAAYAWRHEPAPASRPRRMTLETLKTQTTSPESVAMSKDLKRRGWSFVGPTTV
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHH
YAFMQAMGLVNDHLDGCHVRERALQARRGLQPPR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SPPLPSGLFDTATDGAPRCTWCAATPAYQHYHDHEWGFPVTDDRRLFEKLCLEGFQAGL
CCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SWLTILNKRENFRAAFADFDAERLSRYTGRDVERLLGDAGIVRHRGKIESTLNNARRVIE
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAEFGSLAAYAWRHEPAPASRPRRMTLETLKTQTTSPESVAMSKDLKRRGWSFVGPTTV
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHH
YAFMQAMGLVNDHLDGCHVRERALQARRGLQPPR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]