Click here to switch to the map view.

The map label for this gene is rsmA [H]

Identifier: 124028496

GI number: 124028496

Start: 1649535

End: 1650290

Strand: Reverse

Name: rsmA [H]

Synonym: Hbut_1657

Alternate gene names: 124028496

Gene position: 1650290-1649535 (Counterclockwise)

Preceding gene: 124028497

Following gene: 124028495

Centisome position: 98.99

GC content: 53.17

Gene sequence:

>756_bases
GTGTTAAGAGCTAGGCGCGAACTTGGGCAACACTTCCTCGTTGCAAGGTGGGTTGCGAGAATCTTTGCTGGCTGGGCTTG
TAGGTTTCGGAGGCTACTTGAGGTAGGTGTTGGCCAGGGATTTCTTACCTCGACGATCCTGCGTAGCTGTAGCGTCGAGA
TAGCCGGCCTAGAGCTTGATTTGCGGCTCGTAGGCGAGCTTGCCTCAATAAGCTTTTACTTTACAGGGTTCATGCCCGTC
ATAGCTGATGCTGTGGAGCCTCCGCTAAGACTGGGAGGTGTGGATGCAGTTTATGGAAGTATACCCTACAACATCACGGG
GCCGCTGCTCTCGCTACTAGTTGTCGAGGCTAGGAAACCGGCACTGCTGCTTCTCCAGCGAGAGGTTGTTGATCGGCTGG
CTGCAAAGCCGGGCACAGCAAGCTATGGCAGGATAACGGTTCTCGTGAGGCTCGTCTACGATGTTAAGCCAGGCCCAGTG
GTACCTCCATCAGCTTTCAGGCCTAGGCCAAAGGTGTACTCGAGAATAGTTGAGCTAGTACCCAGGCCTGACGCCCCCAG
CCCAGATATGTTGAGGAGGGTTGAGGAGCTTACCAAGTGCATGTTCTCAGAGAGGAATAAGAGAGCTGCAAAGGTCGCAG
CTAAATGCATTGGAATCGAGCTAAGTAAAATTGAGGGTCTTGTTGGCAGAGATATAAGAGTCTATCAGCTGGAGCCGGAG
AAGTTTATCCAGCTGCTGGACTCCCAGAATGGGTAG

Upstream 100 bases:

>100_bases
GCAAAGCATTGAGTCTAGACCCATCTTCCTAAACTACCTCGCACTAATCTATAAGCGGCTCTTCGGAAGCGAGAGTAGTG
GCGGGGGCTAGGGCTGAGAA

Downstream 100 bases:

>100_bases
GAGCCGTGATAAGGCCCGGCTATAGCTGCCAGCTCCGAGTCACAAGTAACTGCCCACCGCTCGGGGACCTTGTTCTCTCA
TGCTGCGAGAATGTGTATGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLRARRELGQHFLVARWVARIFAGWACRFRRLLEVGVGQGFLTSTILRSCSVEIAGLELDLRLVGELASISFYFTGFMPV
IADAVEPPLRLGGVDAVYGSIPYNITGPLLSLLVVEARKPALLLLQREVVDRLAAKPGTASYGRITVLVRLVYDVKPGPV
VPPSAFRPRPKVYSRIVELVPRPDAPSPDMLRRVEELTKCMFSERNKRAAKVAAKCIGIELSKIEGLVGRDIRVYQLEPE
KFIQLLDSQNG

Sequences:

>Translated_251_residues
MLRARRELGQHFLVARWVARIFAGWACRFRRLLEVGVGQGFLTSTILRSCSVEIAGLELDLRLVGELASISFYFTGFMPV
IADAVEPPLRLGGVDAVYGSIPYNITGPLLSLLVVEARKPALLLLQREVVDRLAAKPGTASYGRITVLVRLVYDVKPGPV
VPPSAFRPRPKVYSRIVELVPRPDAPSPDMLRRVEELTKCMFSERNKRAAKVAAKCIGIELSKIEGLVGRDIRVYQLEPE
KFIQLLDSQNG
>Mature_251_residues
MLRARRELGQHFLVARWVARIFAGWACRFRRLLEVGVGQGFLTSTILRSCSVEIAGLELDLRLVGELASISFYFTGFMPV
IADAVEPPLRLGGVDAVYGSIPYNITGPLLSLLVVEARKPALLLLQREVVDRLAAKPGTASYGRITVLVRLVYDVKPGPV
VPPSAFRPRPKVYSRIVELVPRPDAPSPDMLRRVEELTKCMFSERNKRAAKVAAKCIGIELSKIEGLVGRDIRVYQLEPE
KFIQLLDSQNG

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=209, Percent_Identity=27.7511961722488, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786236, Length=228, Percent_Identity=31.140350877193, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI25146882, Length=188, Percent_Identity=30.8510638297872, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324989, Length=201, Percent_Identity=30.3482587064677, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358017, Length=259, Percent_Identity=29.3436293436293, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27881; Mature: 27881

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRARRELGQHFLVARWVARIFAGWACRFRRLLEVGVGQGFLTSTILRSCSVEIAGLELD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECEEEH
LRLVGELASISFYFTGFMPVIADAVEPPLRLGGVDAVYGSIPYNITGPLLSLLVVEARKP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCC
ALLLLQREVVDRLAAKPGTASYGRITVLVRLVYDVKPGPVVPPSAFRPRPKVYSRIVELV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
PRPDAPSPDMLRRVEELTKCMFSERNKRAAKVAAKCIGIELSKIEGLVGRDIRVYQLEPE
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECHH
KFIQLLDSQNG
HHHHHHHCCCC
>Mature Secondary Structure
MLRARRELGQHFLVARWVARIFAGWACRFRRLLEVGVGQGFLTSTILRSCSVEIAGLELD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECEEEH
LRLVGELASISFYFTGFMPVIADAVEPPLRLGGVDAVYGSIPYNITGPLLSLLVVEARKP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCC
ALLLLQREVVDRLAAKPGTASYGRITVLVRLVYDVKPGPVVPPSAFRPRPKVYSRIVELV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
PRPDAPSPDMLRRVEELTKCMFSERNKRAAKVAAKCIGIELSKIEGLVGRDIRVYQLEPE
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECHH
KFIQLLDSQNG
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]