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The map label for this gene is tuf

Identifier: 124028427

GI number: 124028427

Start: 1590853

End: 1592175

Strand: Direct

Name: tuf

Synonym: Hbut_1581

Alternate gene names: 124028427

Gene position: 1590853-1592175 (Clockwise)

Preceding gene: 124028426

Following gene: 124028428

Centisome position: 95.42

GC content: 52.53

Gene sequence:

>1323_bases
ATGAGCCAGCAGAAGCCACACATAAACCTGGTAGTCATAGGTCACGTAGACCACGGTAAGAGCACGCTGGTAGGCCACCT
ACTATACCGACTAGGCTTCGTCGATGAGAAGACGATAAAGATGCTAGAGGAAGAGGCTAAGAAGAAGGGTAAGGAGTCCT
TCAAGTACGCATGGCTTCTAGACAGGCTAAAGGAGGAGAGAGAGCGCGGCGTAACCATTGACCTAACCTTCGTAAAGTTC
GAGACAAAGAAGTACTACTTCACTATCATCGACGCGCCAGGTCACAGAGACTTCGTAAAGAACATGATCACCGGTGCCAG
CCAGGCCGACGCTGCCATACTAGTAGTATCGGCGAGGAGGGGTGAGTTCGAGGCCGGTATGAGCGCAGAGGGTCAGACAC
GTGAGCACCTAATTCTAGCAAAGACTATGGGTATTGACCAGCTAATCGTTGCTGTAAACAAGATGGATGCAACCGAGCCT
CCATACAGCAAGCAGAGGTACGAGCAGATCGTAGCATTCCTCAAGAAGTTCATGAAGAGCCTAGGCTACAAGGTAGACCA
GATACCATTCATACCAGTATCTGCATGGACTGGCGAGAACCTAATCGAGCGGAGCCCCAACATGCCATGGTACAATGGTC
CAACCCTAGTAGAGGCCCTTGACACCTACATACAGCCACCAAAGAAGCCTGTAGACAAGCCGCTAAGGATACCAATACAG
AACGTCTACTCGATCCCAGGCGTTGGCACTGTACCAGTAGGCAGAGTAGAGACTGGCGTGCTAAAGGTAGGTGACAAGGT
CGTCTTCATGCCACCGGGTGTGGTCGGCGAGGTTAGGAGCATCGAGATGCACCACCAGCCATTACAGCAGGCCGAGCCCG
GCGACAACATCGGCTTCAACGTACGTGGCATATCCAAGAAGGACATTAGGCGTGGCGACGTAGCTGGCCACGTAGACAAG
CCACCAACAGTTGCTGAGGAGTTTACAGCAAGGATCTTCGTCATCTGGCACCCAAGCGCCATCACCGTCGGCTACACACC
AGTAATACACGCACACACCGCCAGCATTGCCGCCAGGATAACTGAGATACAGGCTAAGCTAGACCCGAGAACCGGCCAGG
TAATTGAGAAGAACCCACAGTTCCTAAAGGCCGGCGATGCCGCCATCGTGAAGTTCAAGCCAATTAAGCCAATGGTAATC
GAGAAGTACAGCGAGTTCCCACAGCTAGGCAGGTTCGCAATGAGAGACATGGGTAAGACCATCGGCATCGGCATCGTCGT
AGACGTCAAGCCTGCCAAGGTTGAGATAAGGGCTAAGAAGTAA

Upstream 100 bases:

>100_bases
TGTCTTACTGGCTCGGAGCTACACTTATCCTAAATATTTATAAGACTCTCTTCACCTATGTCAGCCTTCCGGCGTGGAGT
GAAGGTAGCGGGTGCGGATT

Downstream 100 bases:

>100_bases
GCCCAAATAATCTCTTTTACTGTTTCCAGCTACCGCGTTTTTAGCAGTGCGAAACACTCACGTCCGGTTCCGATAACCCT
ATTATCACCTCCATACATCA

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSQQKPHINLVVIGHVDHGKSTLVGHLLYRLGFVDEKTIKMLEEEAKKKGKESFKYAWLLDRLKEERERGVTIDLTFVKF
ETKKYYFTIIDAPGHRDFVKNMITGASQADAAILVVSARRGEFEAGMSAEGQTREHLILAKTMGIDQLIVAVNKMDATEP
PYSKQRYEQIVAFLKKFMKSLGYKVDQIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDTYIQPPKKPVDKPLRIPIQ
NVYSIPGVGTVPVGRVETGVLKVGDKVVFMPPGVVGEVRSIEMHHQPLQQAEPGDNIGFNVRGISKKDIRRGDVAGHVDK
PPTVAEEFTARIFVIWHPSAITVGYTPVIHAHTASIAARITEIQAKLDPRTGQVIEKNPQFLKAGDAAIVKFKPIKPMVI
EKYSEFPQLGRFAMRDMGKTIGIGIVVDVKPAKVEIRAKK

Sequences:

>Translated_440_residues
MSQQKPHINLVVIGHVDHGKSTLVGHLLYRLGFVDEKTIKMLEEEAKKKGKESFKYAWLLDRLKEERERGVTIDLTFVKF
ETKKYYFTIIDAPGHRDFVKNMITGASQADAAILVVSARRGEFEAGMSAEGQTREHLILAKTMGIDQLIVAVNKMDATEP
PYSKQRYEQIVAFLKKFMKSLGYKVDQIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDTYIQPPKKPVDKPLRIPIQ
NVYSIPGVGTVPVGRVETGVLKVGDKVVFMPPGVVGEVRSIEMHHQPLQQAEPGDNIGFNVRGISKKDIRRGDVAGHVDK
PPTVAEEFTARIFVIWHPSAITVGYTPVIHAHTASIAARITEIQAKLDPRTGQVIEKNPQFLKAGDAAIVKFKPIKPMVI
EKYSEFPQLGRFAMRDMGKTIGIGIVVDVKPAKVEIRAKK
>Mature_439_residues
SQQKPHINLVVIGHVDHGKSTLVGHLLYRLGFVDEKTIKMLEEEAKKKGKESFKYAWLLDRLKEERERGVTIDLTFVKFE
TKKYYFTIIDAPGHRDFVKNMITGASQADAAILVVSARRGEFEAGMSAEGQTREHLILAKTMGIDQLIVAVNKMDATEPP
YSKQRYEQIVAFLKKFMKSLGYKVDQIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDTYIQPPKKPVDKPLRIPIQN
VYSIPGVGTVPVGRVETGVLKVGDKVVFMPPGVVGEVRSIEMHHQPLQQAEPGDNIGFNVRGISKKDIRRGDVAGHVDKP
PTVAEEFTARIFVIWHPSAITVGYTPVIHAHTASIAARITEIQAKLDPRTGQVIEKNPQFLKAGDAAIVKFKPIKPMVIE
KYSEFPQLGRFAMRDMGKTIGIGIVVDVKPAKVEIRAKK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=56.8232662192394, Blast_Score=541, Evalue=1e-154,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=55.7562076749436, Blast_Score=537, Evalue=1e-153,
Organism=Homo sapiens, GI223555963, Length=429, Percent_Identity=42.8904428904429, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI5729864, Length=429, Percent_Identity=42.8904428904429, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI194018520, Length=431, Percent_Identity=40.6032482598608, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI194097354, Length=431, Percent_Identity=40.6032482598608, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI194018522, Length=431, Percent_Identity=40.6032482598608, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI46094014, Length=431, Percent_Identity=39.4431554524362, Blast_Score=321, Evalue=8e-88,
Organism=Homo sapiens, GI34147630, Length=437, Percent_Identity=33.4096109839817, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI169162126, Length=140, Percent_Identity=41.4285714285714, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI169162824, Length=140, Percent_Identity=41.4285714285714, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI239752724, Length=140, Percent_Identity=41.4285714285714, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=26.5895953757225, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI45593140, Length=469, Percent_Identity=24.7334754797441, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI82546879, Length=474, Percent_Identity=23.6286919831224, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI4503507, Length=438, Percent_Identity=23.2876712328767, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI157426893, Length=315, Percent_Identity=26.031746031746, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789108, Length=437, Percent_Identity=32.7231121281465, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1790412, Length=434, Percent_Identity=30.184331797235, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789737, Length=434, Percent_Identity=30.184331797235, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI2367247, Length=387, Percent_Identity=25.0645994832041, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1788922, Length=272, Percent_Identity=27.2058823529412, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI48994988, Length=264, Percent_Identity=26.8939393939394, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=53.9503386004515, Blast_Score=517, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=53.9503386004515, Blast_Score=517, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI32566303, Length=443, Percent_Identity=49.2099322799097, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI32566629, Length=430, Percent_Identity=39.5348837209302, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI115532067, Length=426, Percent_Identity=39.2018779342723, Blast_Score=301, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI115532065, Length=426, Percent_Identity=39.2018779342723, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI32566301, Length=170, Percent_Identity=61.1764705882353, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17556456, Length=439, Percent_Identity=33.7129840546697, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI25141371, Length=320, Percent_Identity=32.8125, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17509919, Length=374, Percent_Identity=24.5989304812834, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32566299, Length=64, Percent_Identity=60.9375, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17565438, Length=465, Percent_Identity=22.7956989247312, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17506081, Length=295, Percent_Identity=24.0677966101695, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=259, Percent_Identity=27.027027027027, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=54.5248868778281, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=54.5248868778281, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320377, Length=429, Percent_Identity=38.9277389277389, Blast_Score=303, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=33.184855233853, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324761, Length=437, Percent_Identity=32.0366132723112, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6323320, Length=321, Percent_Identity=27.7258566978193, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324650, Length=320, Percent_Identity=24.0625, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652838, Length=439, Percent_Identity=56.4920273348519, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI17137572, Length=439, Percent_Identity=56.4920273348519, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI45553807, Length=443, Percent_Identity=55.3047404063205, Blast_Score=520, Evalue=1e-148,
Organism=Drosophila melanogaster, GI45553816, Length=443, Percent_Identity=55.3047404063205, Blast_Score=520, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24651721, Length=443, Percent_Identity=55.3047404063205, Blast_Score=520, Evalue=1e-148,
Organism=Drosophila melanogaster, GI17864154, Length=443, Percent_Identity=55.3047404063205, Blast_Score=520, Evalue=1e-148,
Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=39.3518518518519, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI17137380, Length=430, Percent_Identity=36.7441860465116, Blast_Score=301, Evalue=8e-82,
Organism=Drosophila melanogaster, GI281363316, Length=435, Percent_Identity=34.4827586206897, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI17864358, Length=435, Percent_Identity=34.4827586206897, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI19921738, Length=435, Percent_Identity=26.8965517241379, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI19922690, Length=316, Percent_Identity=27.2151898734177, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922512, Length=418, Percent_Identity=24.4019138755981, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24654852, Length=418, Percent_Identity=24.4019138755981, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647048, Length=374, Percent_Identity=24.0641711229947, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24647046, Length=373, Percent_Identity=24.1286863270777, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EF1A_HYPBU (A2BN41)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013747.1
- ProteinModelPortal:   A2BN41
- SMR:   A2BN41
- STRING:   A2BN41
- GeneID:   4781899
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1581
- eggNOG:   arNOG04484
- HOGENOM:   HBG307581
- OMA:   AFIGHVD
- PhylomeDB:   A2BN41
- ProtClustDB:   PRK12317
- BioCyc:   HBUT415426:HBUT_1581-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_A
- InterPro:   IPR000795
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00483

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 49132; Mature: 49001

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQKPHINLVVIGHVDHGKSTLVGHLLYRLGFVDEKTIKMLEEEAKKKGKESFKYAWLL
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
DRLKEERERGVTIDLTFVKFETKKYYFTIIDAPGHRDFVKNMITGASQADAAILVVSARR
HHHHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEEECC
GEFEAGMSAEGQTREHLILAKTMGIDQLIVAVNKMDATEPPYSKQRYEQIVAFLKKFMKS
CCCCCCCCCCCCCHHEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LGYKVDQIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDTYIQPPKKPVDKPLRIPIQ
HCCCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHH
NVYSIPGVGTVPVGRVETGVLKVGDKVVFMPPGVVGEVRSIEMHHQPLQQAEPGDNIGFN
HHCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCHHHHHHHHCHHHHCCCCCCCCEE
VRGISKKDIRRGDVAGHVDKPPTVAEEFTARIFVIWHPSAITVGYTPVIHAHTASIAARI
EECCCHHHCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCEEEECCCCEEEEHHHHHHHHH
TEIQAKLDPRTGQVIEKNPQFLKAGDAAIVKFKPIKPMVIEKYSEFPQLGRFAMRDMGKT
HHHHHHCCCCCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHCCCE
IGIGIVVDVKPAKVEIRAKK
ECEEEEEEECCCEEEEEECC
>Mature Secondary Structure 
SQQKPHINLVVIGHVDHGKSTLVGHLLYRLGFVDEKTIKMLEEEAKKKGKESFKYAWLL
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
DRLKEERERGVTIDLTFVKFETKKYYFTIIDAPGHRDFVKNMITGASQADAAILVVSARR
HHHHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEEECC
GEFEAGMSAEGQTREHLILAKTMGIDQLIVAVNKMDATEPPYSKQRYEQIVAFLKKFMKS
CCCCCCCCCCCCCHHEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LGYKVDQIPFIPVSAWTGENLIERSPNMPWYNGPTLVEALDTYIQPPKKPVDKPLRIPIQ
HCCCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHH
NVYSIPGVGTVPVGRVETGVLKVGDKVVFMPPGVVGEVRSIEMHHQPLQQAEPGDNIGFN
HHCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCHHHHHHHHCHHHHCCCCCCCCEE
VRGISKKDIRRGDVAGHVDKPPTVAEEFTARIFVIWHPSAITVGYTPVIHAHTASIAARI
EECCCHHHCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCEEEECCCCEEEEHHHHHHHHH
TEIQAKLDPRTGQVIEKNPQFLKAGDAAIVKFKPIKPMVIEKYSEFPQLGRFAMRDMGKT
HHHHHHCCCCCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHCCCE
IGIGIVVDVKPAKVEIRAKK
ECEEEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA