Click here to switch to the map view.

The map label for this gene is cysH [C]

Identifier: 124028388

GI number: 124028388

Start: 1551269

End: 1552624

Strand: Direct

Name: cysH [C]

Synonym: Hbut_1541

Alternate gene names: 124028388

Gene position: 1551269-1552624 (Clockwise)

Preceding gene: 124028387

Following gene: 124028390

Centisome position: 93.05

GC content: 57.23

Gene sequence:

>1356_bases
ATGGGCGGGCTTGGGGGGCTGCTAAAGGCGTTAGAGAGGGTTGGCTGGCTGCTAGATCGTAACGTTCCAGTGCTAGGCTG
TAGGCTTGGATCTCGCAGCTGCTGGAATGTGAGAGGCGACTACTGGCTAGCGGGTGGGTTTGAGAAGAGCATTGTCAGCC
AGCTCTTCCACTCTACTTTCGGGGTTAGAGTATCCTTTGATGAGAGGCTTGTGGTTTTTCACCGTATACCAGCACCTCAG
AGCGAGTATGCCATCGAGGTTTTTGCTGAAGCGCTAAGACTCAGCGCAATAGAGTATGTGGCTGGGCGTGGCTGGGTTCT
CCACCCTAGTGGTGCAGCTGCAAGTCTTGCAGCAAGCCTTGGCGCTCCAACCATCGGCGTTTCAGGGCCGCGGGGAAGGC
GGCTTAAGGGTAAACGTGTAAGGCTCCCAGAGTGTAGCATTGGGAGCCGCAACTGGGTCTTGGTATCCGTTGATGGCTGG
GTAGGCCCTGCGAGGGTTGTTGGAGAGTGTACTGTGAAGGTTAAGGACTTGGCTCCAAAGGGTATGAGGCTCCTAGATCC
CGGCAGCATGGAGGAGGCAGTGGAAGTTAACGCGGAGGTAGTAGGCGGCCTAGCCTCTGAGGCTAGGGAGTTTATCAGAC
GCGTCTACGCGAGGTACCAGGCTAGCAGGGGCAGGCTATACGTAGCCTTTAGCGGTGGCTCCGACTCCGCCACAGTCCTC
AGCCTCGCGAGGGAGGCTGTTGGCCCCGAGAACGTGGTAGCAGTATACGTTGACACCGGCATGGAGTTTCCGGAGACACG
AAGGTATGTTGAGAAGGTTACTAGCATCCTGGGTGTAGACCTAGAGGTTGTTGAAGCTGAAGCTGAACCCATCGAGGAGA
TTAGGAGAAGAGGGCTCATGACGAGGGATGACAGGTGGTGTACCCGGCTACTAAAGCTCAAGCCGTTGCGGCGCTTCTAC
CGGTCACGCGGCGTAAGGCTCATACTCGACGGGGCTCGCCGCTGGGAAAGCACGACGAGAGCCTCGACACCTAGGATTGG
CGAGAATCCCCTCATACCTGGCGTCGTGAGGGCACTACCCATACATCACTGGCCACGGCTGGCTGTGCAGCTCTACCTCT
ACGAGAGGGGCATACCATTCAACCACCTTTACAGCAAGGGGCTCACACGCATAGGCTGCATATCATGCCCCGCAATGCAT
CTCTACGAGCTACACATAGCATACCATCTACACCCCTGGTGGTTTGAGAAGCTAGCTGAGGCCGTAGCAGAGCACTACGG
GCTCTCACAGCGCGAAGCCCTGCGGTTGCTATTAGTCGGCTCCTGGAGGAAGGCTGGAGGCCGAAAGGCGTCTTAG

Upstream 100 bases:

>100_bases
GTGACAAGCTGCGCGAGGCCGTAGAGAAGCTGCTAGCTGGCTAGGATAGTGATAAACCGCTATACAGCTGGAGGCTCTGC
CCCGGGTGCAGCTAGGATCC

Downstream 100 bases:

>100_bases
AAGCTGGGCTGCCTCGCGCAGCTCAGCTCCCGCCTTTGCAGCACCACGCAGCTCTAGGGTGTTTGCTATCGCTTCAAGCC
TATCAGCTAGAGCCTCCACA

Product: phosphoadenosine phosphosulfate reductase

Products: H+; Adenosine 3',5'-bisphosphate; Sulfite; Oxidized thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MGGLGGLLKALERVGWLLDRNVPVLGCRLGSRSCWNVRGDYWLAGGFEKSIVSQLFHSTFGVRVSFDERLVVFHRIPAPQ
SEYAIEVFAEALRLSAIEYVAGRGWVLHPSGAAASLAASLGAPTIGVSGPRGRRLKGKRVRLPECSIGSRNWVLVSVDGW
VGPARVVGECTVKVKDLAPKGMRLLDPGSMEEAVEVNAEVVGGLASEAREFIRRVYARYQASRGRLYVAFSGGSDSATVL
SLAREAVGPENVVAVYVDTGMEFPETRRYVEKVTSILGVDLEVVEAEAEPIEEIRRRGLMTRDDRWCTRLLKLKPLRRFY
RSRGVRLILDGARRWESTTRASTPRIGENPLIPGVVRALPIHHWPRLAVQLYLYERGIPFNHLYSKGLTRIGCISCPAMH
LYELHIAYHLHPWWFEKLAEAVAEHYGLSQREALRLLLVGSWRKAGGRKAS

Sequences:

>Translated_451_residues
MGGLGGLLKALERVGWLLDRNVPVLGCRLGSRSCWNVRGDYWLAGGFEKSIVSQLFHSTFGVRVSFDERLVVFHRIPAPQ
SEYAIEVFAEALRLSAIEYVAGRGWVLHPSGAAASLAASLGAPTIGVSGPRGRRLKGKRVRLPECSIGSRNWVLVSVDGW
VGPARVVGECTVKVKDLAPKGMRLLDPGSMEEAVEVNAEVVGGLASEAREFIRRVYARYQASRGRLYVAFSGGSDSATVL
SLAREAVGPENVVAVYVDTGMEFPETRRYVEKVTSILGVDLEVVEAEAEPIEEIRRRGLMTRDDRWCTRLLKLKPLRRFY
RSRGVRLILDGARRWESTTRASTPRIGENPLIPGVVRALPIHHWPRLAVQLYLYERGIPFNHLYSKGLTRIGCISCPAMH
LYELHIAYHLHPWWFEKLAEAVAEHYGLSQREALRLLLVGSWRKAGGRKAS
>Mature_450_residues
GGLGGLLKALERVGWLLDRNVPVLGCRLGSRSCWNVRGDYWLAGGFEKSIVSQLFHSTFGVRVSFDERLVVFHRIPAPQS
EYAIEVFAEALRLSAIEYVAGRGWVLHPSGAAASLAASLGAPTIGVSGPRGRRLKGKRVRLPECSIGSRNWVLVSVDGWV
GPARVVGECTVKVKDLAPKGMRLLDPGSMEEAVEVNAEVVGGLASEAREFIRRVYARYQASRGRLYVAFSGGSDSATVLS
LAREAVGPENVVAVYVDTGMEFPETRRYVEKVTSILGVDLEVVEAEAEPIEEIRRRGLMTRDDRWCTRLLKLKPLRRFYR
SRGVRLILDGARRWESTTRASTPRIGENPLIPGVVRALPIHHWPRLAVQLYLYERGIPFNHLYSKGLTRIGCISCPAMHL
YELHIAYHLHPWWFEKLAEAVAEHYGLSQREALRLLLVGSWRKAGGRKAS

Specific function: Reduction Of Activated Sulfate Into Sulfite. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR014729
- InterPro:   IPR004521 [H]

Pfam domain/function: PF01507 PAPS_reduct; PF01472 PUA [H]

EC number: 1.8.4.8 [C]

Molecular weight: Translated: 50200; Mature: 50069

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLGGLLKALERVGWLLDRNVPVLGCRLGSRSCWNVRGDYWLAGGFEKSIVSQLFHSTF
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHC
GVRVSFDERLVVFHRIPAPQSEYAIEVFAEALRLSAIEYVAGRGWVLHPSGAAASLAASL
CEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHC
GAPTIGVSGPRGRRLKGKRVRLPECSIGSRNWVLVSVDGWVGPARVVGECTVKVKDLAPK
CCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEECCCCCHHHHHEEEEEEEECCCCC
GMRLLDPGSMEEAVEVNAEVVGGLASEAREFIRRVYARYQASRGRLYVAFSGGSDSATVL
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
SLAREAVGPENVVAVYVDTGMEFPETRRYVEKVTSILGVDLEVVEAEAEPIEEIRRRGLM
HHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCC
TRDDRWCTRLLKLKPLRRFYRSRGVRLILDGARRWESTTRASTPRIGENPLIPGVVRALP
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHCCHHCCCCCCCCCCCCCCHHHHHHC
IHHWPRLAVQLYLYERGIPFNHLYSKGLTRIGCISCPAMHLYELHIAYHLHPWWFEKLAE
CCCCHHHHEEEEEHHCCCCHHHHHHHCCCEEECCCCCHHHHEEEEEEEEECHHHHHHHHH
AVAEHYGLSQREALRLLLVGSWRKAGGRKAS
HHHHHCCCCHHHHEEEEEEECHHHCCCCCCC
>Mature Secondary Structure 
GGLGGLLKALERVGWLLDRNVPVLGCRLGSRSCWNVRGDYWLAGGFEKSIVSQLFHSTF
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHC
GVRVSFDERLVVFHRIPAPQSEYAIEVFAEALRLSAIEYVAGRGWVLHPSGAAASLAASL
CEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHC
GAPTIGVSGPRGRRLKGKRVRLPECSIGSRNWVLVSVDGWVGPARVVGECTVKVKDLAPK
CCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEECCCCCHHHHHEEEEEEEECCCCC
GMRLLDPGSMEEAVEVNAEVVGGLASEAREFIRRVYARYQASRGRLYVAFSGGSDSATVL
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
SLAREAVGPENVVAVYVDTGMEFPETRRYVEKVTSILGVDLEVVEAEAEPIEEIRRRGLM
HHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCC
TRDDRWCTRLLKLKPLRRFYRSRGVRLILDGARRWESTTRASTPRIGENPLIPGVVRALP
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHCCHHCCCCCCCCCCCCCCHHHHHHC
IHHWPRLAVQLYLYERGIPFNHLYSKGLTRIGCISCPAMHLYELHIAYHLHPWWFEKLAE
CCCCHHHHEEEEEHHCCCCHHHHHHHCCCEEECCCCCHHHHEEEEEEEEECHHHHHHHHH
AVAEHYGLSQREALRLLLVGSWRKAGGRKAS
HHHHHCCCCHHHHEEEEEEECHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 5700 [C]

Specific activity: 99

Km value (mM): 0.01 {5-phosphoadenosine} [C]

Substrates: 3'-Phosphoadenylyl sulfate; Reduced thioredoxin [C]

Specific reaction: 3'-Phosphoadenylyl sulfate + Reduced thioredoxin --> (2) H+ + Adenosine 3',5'-bisphosphate + Sulfite + Oxidized thioredoxin [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: Adenosine3', 5'-bisphosphate; N-Bromosuccinimide; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]