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The map label for this gene is fabH [C]

Identifier: 124028374

GI number: 124028374

Start: 1534330

End: 1535385

Strand: Direct

Name: fabH [C]

Synonym: Hbut_1527

Alternate gene names: 124028374

Gene position: 1534330-1535385 (Clockwise)

Preceding gene: 124028365

Following gene: 124028375

Centisome position: 92.03

GC content: 57.48

Gene sequence:

>1056_bases
ATGCCGCGTGGAAGCGGCATAGTTGGCTGGGGCGGCTACGTGCCCCGTTACAGGATCAAGGCTGCGGAGATTGTAAGGGT
TTGGGGCTGGGAGCCTAGCGTTCCCGCAGGGCTCGGGGTGAAGGAGAAGGCTGTGGAGAATGTTGACGAGGATTCAGTCA
CAATGGGTTATGAGGCTGCGCGGAACGCAATAGCACGAGCCAACGTAGACCCCAGGGAGATAAAGGCCGTGTTCTTCGGC
ACCGAGTCCAAGCCATACGCTGTCAAGCCCTCTGCCACAATTATCGCCGAGGCTCTCGGTATAACACCAGAGACCATGGC
GTCGGACCTGGAGTTTGCATGCCGCGCTGCCAGCGAGGGGCTCCGAGCGAGCCTTGCACTAGTAGAGGCTGGCTACATGA
AGTACGCACTGGTAGTAGCTTCCGATACTGCGCAAGCAAACCCGGGCGACGTGCTAGAATTTACTGCTGCCAGCGGCGCA
GCAGCCTTTGTTGTCGGCCCTGCATCGGAAAGCGTTGCCGTGCTCGAAGGGGTCTACACCTACGTCACTGACACACCGGA
CTTCTGGAGGGGCCAGCACAGCCGCTACCCAATGCACGGCGAGGCATTCACCGGCGAGCCAGCATACTTCCACCACATAG
AGAGCGCAGTCAAGGGCTTAATGGAGAAGCTCGGGCTAAAACCCGAGGACTTTGACTACGCCGTCTTCCATCAGCCTAAC
GGTAAGTTCCCCCTAAGGGTTGGAGCAAGACTCGGCTTCCCCAAGGAGAAGATTCTGCCAGGCCTCCTCACACCAATAAT
CGGCAACACATACAATGCCAGTGCCCTACTAGGCTTCGCAAGGATACTCGATCAGGCAAAGCCTGGCCAGCGAATACTCG
TAGCACCATTCGGCAGTGGCGCGGGTAGCGACGCATATAGCTTCATCGTAACCGATCGCATCGAGGAGGCTAGGAATCGT
GCACCAAAGGTTGACGACTACGTGAACTGGAAGCGCTATATAGACTATGCTATGCACGCACGCATGCGCAAGCTCTATGA
CAGAAGACCCGTCTAG

Upstream 100 bases:

>100_bases
CGGCGATAGTCGAATAGTACTCTAGGCAGTTGTTGTAGAGGGAGGCTTCTTTTAGCTATGCAGCTGCCTCGTACCCGAGC
AACTGGCAGCGTGAAGCGGC

Downstream 100 bases:

>100_bases
TCGTGGCGGGGTGATGATTATGCCTCGAGTCTACGTAGCCGGAGTCGGGATGACAAAGATCGACCGTCACTACGACAAGG
GTATCCTGGACCTAGCAGCC

Product: hypothetical protein

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MPRGSGIVGWGGYVPRYRIKAAEIVRVWGWEPSVPAGLGVKEKAVENVDEDSVTMGYEAARNAIARANVDPREIKAVFFG
TESKPYAVKPSATIIAEALGITPETMASDLEFACRAASEGLRASLALVEAGYMKYALVVASDTAQANPGDVLEFTAASGA
AAFVVGPASESVAVLEGVYTYVTDTPDFWRGQHSRYPMHGEAFTGEPAYFHHIESAVKGLMEKLGLKPEDFDYAVFHQPN
GKFPLRVGARLGFPKEKILPGLLTPIIGNTYNASALLGFARILDQAKPGQRILVAPFGSGAGSDAYSFIVTDRIEEARNR
APKVDDYVNWKRYIDYAMHARMRKLYDRRPV

Sequences:

>Translated_351_residues
MPRGSGIVGWGGYVPRYRIKAAEIVRVWGWEPSVPAGLGVKEKAVENVDEDSVTMGYEAARNAIARANVDPREIKAVFFG
TESKPYAVKPSATIIAEALGITPETMASDLEFACRAASEGLRASLALVEAGYMKYALVVASDTAQANPGDVLEFTAASGA
AAFVVGPASESVAVLEGVYTYVTDTPDFWRGQHSRYPMHGEAFTGEPAYFHHIESAVKGLMEKLGLKPEDFDYAVFHQPN
GKFPLRVGARLGFPKEKILPGLLTPIIGNTYNASALLGFARILDQAKPGQRILVAPFGSGAGSDAYSFIVTDRIEEARNR
APKVDDYVNWKRYIDYAMHARMRKLYDRRPV
>Mature_350_residues
PRGSGIVGWGGYVPRYRIKAAEIVRVWGWEPSVPAGLGVKEKAVENVDEDSVTMGYEAARNAIARANVDPREIKAVFFGT
ESKPYAVKPSATIIAEALGITPETMASDLEFACRAASEGLRASLALVEAGYMKYALVVASDTAQANPGDVLEFTAASGAA
AFVVGPASESVAVLEGVYTYVTDTPDFWRGQHSRYPMHGEAFTGEPAYFHHIESAVKGLMEKLGLKPEDFDYAVFHQPNG
KFPLRVGARLGFPKEKILPGLLTPIIGNTYNASALLGFARILDQAKPGQRILVAPFGSGAGSDAYSFIVTDRIEEARNRA
PKVDDYVNWKRYIDYAMHARMRKLYDRRPV

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Catalyzes The First Condensation Reaction Which Initiates Fatty Acid Synthesis And May Therefore Play A Role In Governing The

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0219 family [H]

Homologues:

Organism=Escherichia coli, GI1787333, Length=316, Percent_Identity=27.5316455696203, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013747
- InterPro:   IPR004656
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08541 ACP_syn_III_C [H]

EC number: 2.3.1.41

Molecular weight: Translated: 38242; Mature: 38111

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGSGIVGWGGYVPRYRIKAAEIVRVWGWEPSVPAGLGVKEKAVENVDEDSVTMGYEAA
CCCCCEEEECCCCCCCEEECHHHEEEEECCCCCCCCCCCCHHHHHHCCCCCCEEECHHHH
RNAIARANVDPREIKAVFFGTESKPYAVKPSATIIAEALGITPETMASDLEFACRAASEG
HHHHHHCCCCHHHEEEEEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LRASLALVEAGYMKYALVVASDTAQANPGDVLEFTAASGAAAFVVGPASESVAVLEGVYT
HHHHHHHHHHCCEEEEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHH
YVTDTPDFWRGQHSRYPMHGEAFTGEPAYFHHIESAVKGLMEKLGLKPEDFDYAVFHQPN
EECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
GKFPLRVGARLGFPKEKILPGLLTPIIGNTYNASALLGFARILDQAKPGQRILVAPFGSG
CCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCC
AGSDAYSFIVTDRIEEARNRAPKVDDYVNWKRYIDYAMHARMRKLYDRRPV
CCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRGSGIVGWGGYVPRYRIKAAEIVRVWGWEPSVPAGLGVKEKAVENVDEDSVTMGYEAA
CCCCEEEECCCCCCCEEECHHHEEEEECCCCCCCCCCCCHHHHHHCCCCCCEEECHHHH
RNAIARANVDPREIKAVFFGTESKPYAVKPSATIIAEALGITPETMASDLEFACRAASEG
HHHHHHCCCCHHHEEEEEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LRASLALVEAGYMKYALVVASDTAQANPGDVLEFTAASGAAAFVVGPASESVAVLEGVYT
HHHHHHHHHHCCEEEEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHH
YVTDTPDFWRGQHSRYPMHGEAFTGEPAYFHHIESAVKGLMEKLGLKPEDFDYAVFHQPN
EECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
GKFPLRVGARLGFPKEKILPGLLTPIIGNTYNASALLGFARILDQAKPGQRILVAPFGSG
CCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCC
AGSDAYSFIVTDRIEEARNRAPKVDDYVNWKRYIDYAMHARMRKLYDRRPV
CCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]