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The map label for this gene is panB

Identifier: 124028370

GI number: 124028370

Start: 1531053

End: 1531919

Strand: Reverse

Name: panB

Synonym: Hbut_1523

Alternate gene names: 124028370

Gene position: 1531919-1531053 (Counterclockwise)

Preceding gene: 124028371

Following gene: 124028369

Centisome position: 91.89

GC content: 56.52

Gene sequence:

>867_bases
ATGGCTGGCCGGGAGAAGGTTACCGTGCGAGATATAGTTCGTGCTAAGCAGCGCGGAGAGCGCATAGTAATGGTTACGGC
TTACGACTACATAACAGCAAAGCTCGTCGACGAAGCCGGCGTAGATATGATTCTTGTGGGAGACTCTCTCGGCATGGTTG
TTCTAGGTCTCCCTTCTACTCACCAGGTCACCCTTGAGGACATGGAGAGGCATACAGCAGCTGTTGCAAGAGCCCAGCCC
CGAGCCCTAATCGTCGCCGATATGCCGTTTATGAGCTACGAGGCCTCAACCCGGGACGCGGTACTGAATGCTGGCAGGCT
AATTGCAGCCGGGGCTGATGCAGTGAAGATAGAGGGCGGGGCCAGCTACAGCGACACCATAAGAGCATTGGTGAGGGCGG
GTATACCAGTCGTGGCCCATGTGGGGCTTACCCCGCAACGCTACAAGCTGCTCGGTGGCTACCGCCTGGCAGGCAAAACG
GCCAGCGAAGCCATGGAGGTTATCCGGGAGGCTATAGGTGCAGAGGAGGCTGGCGCCTTTGCTGTAGTCATAGAGTTTAC
GGCGTGGGAAGTAGCGAGGGAGATAACGAGGAAACTCTCAATACCAACTATATGTATCGGTAGTGGTCCCTACTGTGACG
GCCAGGTACTGGTAATCCATGATCTCCTCGGCCTAACCCCGACCCCGCCACCGTTCGCCAAGAAATATGTTGACCTTGCC
GCAATTATTCGGAGAGCCGTCAGCGAGTATGCCTCGGATGTACGCAATGGCAGGTTCCCCGGCGAAGGCATGTACTGGGG
GATGAAGAGGGGCGAGTATGAGAAGCTCCAACGCCTCATCAACGAGCAGGCTGGTTCTGGAGATTAG

Upstream 100 bases:

>100_bases
TAGACTATATCAATGGCTCGGTAGCCCGTGAGGCTTGGAGGCTCGGGCTAAGGGCACCATTCAACGAAGCTATGTGGAGA
GCTGTGAGGTTGCTAGAGCG

Downstream 100 bases:

>100_bases
CGGTGTAGAGGAGGCCACCCTGCTAGCCAGGCCCAACAGGTTTACAGCTGTGCTAGAAGCTGCGGGAAGAGAGTTCACAT
GCCACATACATGATCCGGGT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MAGREKVTVRDIVRAKQRGERIVMVTAYDYITAKLVDEAGVDMILVGDSLGMVVLGLPSTHQVTLEDMERHTAAVARAQP
RALIVADMPFMSYEASTRDAVLNAGRLIAAGADAVKIEGGASYSDTIRALVRAGIPVVAHVGLTPQRYKLLGGYRLAGKT
ASEAMEVIREAIGAEEAGAFAVVIEFTAWEVAREITRKLSIPTICIGSGPYCDGQVLVIHDLLGLTPTPPPFAKKYVDLA
AIIRRAVSEYASDVRNGRFPGEGMYWGMKRGEYEKLQRLINEQAGSGD

Sequences:

>Translated_288_residues
MAGREKVTVRDIVRAKQRGERIVMVTAYDYITAKLVDEAGVDMILVGDSLGMVVLGLPSTHQVTLEDMERHTAAVARAQP
RALIVADMPFMSYEASTRDAVLNAGRLIAAGADAVKIEGGASYSDTIRALVRAGIPVVAHVGLTPQRYKLLGGYRLAGKT
ASEAMEVIREAIGAEEAGAFAVVIEFTAWEVAREITRKLSIPTICIGSGPYCDGQVLVIHDLLGLTPTPPPFAKKYVDLA
AIIRRAVSEYASDVRNGRFPGEGMYWGMKRGEYEKLQRLINEQAGSGD
>Mature_287_residues
AGREKVTVRDIVRAKQRGERIVMVTAYDYITAKLVDEAGVDMILVGDSLGMVVLGLPSTHQVTLEDMERHTAAVARAQPR
ALIVADMPFMSYEASTRDAVLNAGRLIAAGADAVKIEGGASYSDTIRALVRAGIPVVAHVGLTPQRYKLLGGYRLAGKTA
SEAMEVIREAIGAEEAGAFAVVIEFTAWEVAREITRKLSIPTICIGSGPYCDGQVLVIHDLLGLTPTPPPFAKKYVDLAA
IIRRAVSEYASDVRNGRFPGEGMYWGMKRGEYEKLQRLINEQAGSGD

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=256, Percent_Identity=42.96875, Blast_Score=205, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319653, Length=286, Percent_Identity=37.4125874125874, Blast_Score=189, Evalue=6e-49,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_HYPBU (A2BMY4)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013690.1
- ProteinModelPortal:   A2BMY4
- SMR:   A2BMY4
- STRING:   A2BMY4
- GeneID:   4782815
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1523
- eggNOG:   arNOG04418
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   A2BMY4
- ProtClustDB:   CLSK2785329
- BioCyc:   HBUT415426:HBUT_1523-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 31092; Mature: 30961

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: ACT_SITE 185-185 BINDING 87-87 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGREKVTVRDIVRAKQRGERIVMVTAYDYITAKLVDEAGVDMILVGDSLGMVVLGLPST
CCCCCCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCC
HQVTLEDMERHTAAVARAQPRALIVADMPFMSYEASTRDAVLNAGRLIAAGADAVKIEGG
CEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEECCCEEEECCC
ASYSDTIRALVRAGIPVVAHVGLTPQRYKLLGGYRLAGKTASEAMEVIREAIGAEEAGAF
CCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCE
AVVIEFTAWEVAREITRKLSIPTICIGSGPYCDGQVLVIHDLLGLTPTPPPFAKKYVDLA
EEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHH
AIIRRAVSEYASDVRNGRFPGEGMYWGMKRGEYEKLQRLINEQAGSGD
HHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AGREKVTVRDIVRAKQRGERIVMVTAYDYITAKLVDEAGVDMILVGDSLGMVVLGLPST
CCCCCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCC
HQVTLEDMERHTAAVARAQPRALIVADMPFMSYEASTRDAVLNAGRLIAAGADAVKIEGG
CEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEECCCEEEECCC
ASYSDTIRALVRAGIPVVAHVGLTPQRYKLLGGYRLAGKTASEAMEVIREAIGAEEAGAF
CCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCE
AVVIEFTAWEVAREITRKLSIPTICIGSGPYCDGQVLVIHDLLGLTPTPPPFAKKYVDLA
EEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHH
AIIRRAVSEYASDVRNGRFPGEGMYWGMKRGEYEKLQRLINEQAGSGD
HHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA