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The map label for this gene is hmeA [H]

Identifier: 124028242

GI number: 124028242

Start: 1390778

End: 1391194

Strand: Direct

Name: hmeA [H]

Synonym: Hbut_1393

Alternate gene names: 124028242

Gene position: 1390778-1391194 (Clockwise)

Preceding gene: 124028241

Following gene: 124028243

Centisome position: 83.42

GC content: 58.03

Gene sequence:

>417_bases
GTGATTGACTATGACCGCTGCATAGGCTGTAGATACTGTATAACAGCATGTCCCTACGGTGCACGCCGCTTCAACTGGGC
CAAGCCCTATGTGCCAGTGACAGAGCTAAACCCCAACATGCACATACTCGGCAACGTGCCACGCCAGATACACGTCGTGG
AGAAGTGTACCTGGTGCATACAGCGCACCCGCGATGGCGGCGTACCGGCGTGCGTCGAGGTCTGCCCTGTTGGTGCGAGA
GTCTTCGGAGACCTCAACGACCCTGAGAGTCCGATACGCCGCGTCCTAAGAGAGTACGGTGGCTTCGTATTGAAGCCCGA
GGCTGGCACCAAGCCCAGATTCTTCTACTTCTTTGGCCCCAAGCGTACACCTCCACGCGAATCCACGGAGACAAGGGCAG
CTCAGGGACAAGGGTAG

Upstream 100 bases:

>100_bases
CAAGGTCTACGTGCCAGTAGCATGTATGCACTGCGAGAACCCGCCGTGTGTCATGGTTTGCCCGGTTAGGGCTACCTGGA
AGGAGCCCGATGGCATTGTA

Downstream 100 bases:

>100_bases
ACCTCTCCGAGGGGGTGTGCCTGGATGACGCGTAGCGGTGGCTTCCTAGACACGATAAAGTACGTTATCCGGGAGGCGCT
ACTAACTGGTAGCCCACGCT

Product: Fe-S-cluster-containing hydrogenase

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MIDYDRCIGCRYCITACPYGARRFNWAKPYVPVTELNPNMHILGNVPRQIHVVEKCTWCIQRTRDGGVPACVEVCPVGAR
VFGDLNDPESPIRRVLREYGGFVLKPEAGTKPRFFYFFGPKRTPPRESTETRAAQGQG

Sequences:

>Translated_138_residues
MIDYDRCIGCRYCITACPYGARRFNWAKPYVPVTELNPNMHILGNVPRQIHVVEKCTWCIQRTRDGGVPACVEVCPVGAR
VFGDLNDPESPIRRVLREYGGFVLKPEAGTKPRFFYFFGPKRTPPRESTETRAAQGQG
>Mature_138_residues
MIDYDRCIGCRYCITACPYGARRFNWAKPYVPVTELNPNMHILGNVPRQIHVVEKCTWCIQRTRDGGVPACVEVCPVGAR
VFGDLNDPESPIRRVLREYGGFVLKPEAGTKPRFFYFFGPKRTPPRESTETRAAQGQG

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=115, Percent_Identity=35.6521739130435, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789370, Length=85, Percent_Identity=35.2941176470588, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 15637; Mature: 15637

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYDRCIGCRYCITACPYGARRFNWAKPYVPVTELNPNMHILGNVPRQIHVVEKCTWCI
CCCCHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEECCCCEEEEECCCCEEHHHHHHHHHH
QRTRDGGVPACVEVCPVGARVFGDLNDPESPIRRVLREYGGFVLKPEAGTKPRFFYFFGP
HHCCCCCCCHHHHHCCCCHHEECCCCCCHHHHHHHHHHHCCEEECCCCCCCCEEEEEECC
KRTPPRESTETRAAQGQG
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDYDRCIGCRYCITACPYGARRFNWAKPYVPVTELNPNMHILGNVPRQIHVVEKCTWCI
CCCCHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEECCCCEEEEECCCCEEHHHHHHHHHH
QRTRDGGVPACVEVCPVGARVFGDLNDPESPIRRVLREYGGFVLKPEAGTKPRFFYFFGP
HHCCCCCCCHHHHHCCCCHHEECCCCCCHHHHHHHHHHHCCEEECCCCCCCCEEEEEECC
KRTPPRESTETRAAQGQG
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]