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The map label for this gene is fen

Identifier: 124028216

GI number: 124028216

Start: 1356757

End: 1357809

Strand: Reverse

Name: fen

Synonym: Hbut_1366

Alternate gene names: 124028216

Gene position: 1357809-1356757 (Counterclockwise)

Preceding gene: 124028217

Following gene: 124028215

Centisome position: 81.44

GC content: 52.8

Gene sequence:

>1053_bases
TTGGGAGTTAATATCAGGGAGGTTATCCCACCGGAGGCTATTCAGGAGATAGACCTTGCTCATCTAAAATACAAGGTCGT
AGCTATTGACGCATATAACGCGCTCTACCAGTTCCTCACAGCTATAAGACAGCCCGATGGAACACCACTCATGGACTCCA
AGGGCCGGATTACAAGCCATCTCAGCGGCCTATTCTACCGGACCATAAACCTAATGGAGCACGGAATTAAGATAGTCTAC
GTTTTTGATGGAAAGCCACCCGAGATGAAGTATCTCGAGATTGAGAGGCGCAAGCGGGTGAAGAGCGAGGCTGTAAAGAA
GTACGAGGAAGCTGTGAAGAAGGGTGATACAAAAGCTGCAAGGCGCTATGCACAGATGGCTGCTAGGCTTACCGACGAAA
TGGTAGAGGATGCTAAGAAGCTGCTTGACGCTATGGGGGTTCCATGGGTGCAGGCTCCCGCCGAGGGTGAAGCCCAGGCA
GCATTCATGGCTCGGCGTGGCGATGCATGGGCCGCTGCAAGCCAGGACTACGACTCTCTCCTCTTCGGCTCCCCGAGGCT
CGTAAGAAACCTTGCAATTACTGGTAGGCGTAAGCTGCCACGAAAAGACGTCTATGTCGAGATCAAGCCGGAACTCATAG
AGCTGGATAAGCTTCTAAAGGCCTTGGGGATTACCAGGGAGCAGCTCGTAGCGCTAGGCATACTGATAGGAACAGACTAC
AACCCCGACGGTGTAAAGGGGATTGGTCCTAAGACAGCATTGAAAATGGTGAAGGCTCACCGTGACCCTGTAAAGCTTCT
ACAAGGCCTTCCTAGGCACGAGTTTCCAACGGATCCACTGAAGATCTACGAGTACTTCCTCAATCCGCCAGTAACGACCG
ACTATAAGCTTGAGTGGAGGGAGCCCGACGAGAAGAAGGTGTTCGAGATTCTAGTCGAGGGGCATGACTTCAGCCCGGAG
AGGGTCAGGAATGCGCTTGAGCGGCTCAAGAAGGCGTATAGGGAGCATTTCCGGGGCCAGCAAATGGGGCTTGACGCCTG
GCTACGAAGGTAA

Upstream 100 bases:

>100_bases
TGTTAACGTGTTGAAGGAGAATCTCAGAAACGTTGAGCTCATAGCCAAGTCTTAAAGCAGTCCCACCAGGCATCCTACCT
AGCAGGGGCTAGGTGGAGCC

Downstream 100 bases:

>100_bases
ATACCATATATACGCATCATAGTTATTTTGCTTATCCACTACCAATATGGCGTAGCTCCTTACTTCTAGCATCCATAAAG
CCATAAGCTGGCCCAGTCCA

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MGVNIREVIPPEAIQEIDLAHLKYKVVAIDAYNALYQFLTAIRQPDGTPLMDSKGRITSHLSGLFYRTINLMEHGIKIVY
VFDGKPPEMKYLEIERRKRVKSEAVKKYEEAVKKGDTKAARRYAQMAARLTDEMVEDAKKLLDAMGVPWVQAPAEGEAQA
AFMARRGDAWAAASQDYDSLLFGSPRLVRNLAITGRRKLPRKDVYVEIKPELIELDKLLKALGITREQLVALGILIGTDY
NPDGVKGIGPKTALKMVKAHRDPVKLLQGLPRHEFPTDPLKIYEYFLNPPVTTDYKLEWREPDEKKVFEILVEGHDFSPE
RVRNALERLKKAYREHFRGQQMGLDAWLRR

Sequences:

>Translated_350_residues
MGVNIREVIPPEAIQEIDLAHLKYKVVAIDAYNALYQFLTAIRQPDGTPLMDSKGRITSHLSGLFYRTINLMEHGIKIVY
VFDGKPPEMKYLEIERRKRVKSEAVKKYEEAVKKGDTKAARRYAQMAARLTDEMVEDAKKLLDAMGVPWVQAPAEGEAQA
AFMARRGDAWAAASQDYDSLLFGSPRLVRNLAITGRRKLPRKDVYVEIKPELIELDKLLKALGITREQLVALGILIGTDY
NPDGVKGIGPKTALKMVKAHRDPVKLLQGLPRHEFPTDPLKIYEYFLNPPVTTDYKLEWREPDEKKVFEILVEGHDFSPE
RVRNALERLKKAYREHFRGQQMGLDAWLRR
>Mature_349_residues
GVNIREVIPPEAIQEIDLAHLKYKVVAIDAYNALYQFLTAIRQPDGTPLMDSKGRITSHLSGLFYRTINLMEHGIKIVYV
FDGKPPEMKYLEIERRKRVKSEAVKKYEEAVKKGDTKAARRYAQMAARLTDEMVEDAKKLLDAMGVPWVQAPAEGEAQAA
FMARRGDAWAAASQDYDSLLFGSPRLVRNLAITGRRKLPRKDVYVEIKPELIELDKLLKALGITREQLVALGILIGTDYN
PDGVKGIGPKTALKMVKAHRDPVKLLQGLPRHEFPTDPLKIYEYFLNPPVTTDYKLEWREPDEKKVFEILVEGHDFSPER
VRNALERLKKAYREHFRGQQMGLDAWLRR

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=338, Percent_Identity=36.094674556213, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI194018535, Length=259, Percent_Identity=29.7297297297297, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194018531, Length=259, Percent_Identity=29.7297297297297, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI284172361, Length=259, Percent_Identity=25.8687258687259, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI284055278, Length=259, Percent_Identity=25.8687258687259, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI284055276, Length=259, Percent_Identity=25.8687258687259, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17510005, Length=336, Percent_Identity=36.9047619047619, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322736, Length=346, Percent_Identity=37.8612716763006, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321697, Length=131, Percent_Identity=28.2442748091603, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320469, Length=263, Percent_Identity=26.2357414448669, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324607, Length=246, Percent_Identity=26.4227642276423, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17647423, Length=347, Percent_Identity=38.328530259366, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24658219, Length=205, Percent_Identity=25.8536585365854, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17137168, Length=252, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_HYPBU (A2BMI0)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013536.1
- ProteinModelPortal:   A2BMI0
- SMR:   A2BMI0
- STRING:   A2BMI0
- GeneID:   4782618
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1366
- eggNOG:   arNOG04505
- HOGENOM:   HBG316651
- OMA:   DYDSLLF
- PhylomeDB:   A2BMI0
- ProtClustDB:   PRK03980
- BioCyc:   HBUT415426:HBUT_1366-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 40038; Mature: 39907

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVNIREVIPPEAIQEIDLAHLKYKVVAIDAYNALYQFLTAIRQPDGTPLMDSKGRITSH
CCCCCHHCCCHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
LSGLFYRTINLMEHGIKIVYVFDGKPPEMKYLEIERRKRVKSEAVKKYEEAVKKGDTKAA
HHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RRYAQMAARLTDEMVEDAKKLLDAMGVPWVQAPAEGEAQAAFMARRGDAWAAASQDYDSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCCHHCCCCCHHHH
LFGSPRLVRNLAITGRRKLPRKDVYVEIKPELIELDKLLKALGITREQLVALGILIGTDY
HCCCHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHEECCC
NPDGVKGIGPKTALKMVKAHRDPVKLLQGLPRHEFPTDPLKIYEYFLNPPVTTDYKLEWR
CCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECC
EPDEKKVFEILVEGHDFSPERVRNALERLKKAYREHFRGQQMGLDAWLRR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure 
GVNIREVIPPEAIQEIDLAHLKYKVVAIDAYNALYQFLTAIRQPDGTPLMDSKGRITSH
CCCCHHCCCHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
LSGLFYRTINLMEHGIKIVYVFDGKPPEMKYLEIERRKRVKSEAVKKYEEAVKKGDTKAA
HHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RRYAQMAARLTDEMVEDAKKLLDAMGVPWVQAPAEGEAQAAFMARRGDAWAAASQDYDSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCCHHCCCCCHHHH
LFGSPRLVRNLAITGRRKLPRKDVYVEIKPELIELDKLLKALGITREQLVALGILIGTDY
HCCCHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHEECCC
NPDGVKGIGPKTALKMVKAHRDPVKLLQGLPRHEFPTDPLKIYEYFLNPPVTTDYKLEWR
CCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECC
EPDEKKVFEILVEGHDFSPERVRNALERLKKAYREHFRGQQMGLDAWLRR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA