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The map label for this gene is truB [H]

Identifier: 124028184

GI number: 124028184

Start: 1324516

End: 1325553

Strand: Direct

Name: truB [H]

Synonym: Hbut_1329

Alternate gene names: 124028184

Gene position: 1324516-1325553 (Clockwise)

Preceding gene: 124028183

Following gene: 124028185

Centisome position: 79.45

GC content: 55.49

Gene sequence:

>1038_bases
TTGTCCAGCGAGCTTACTAGAAAGGGGCTAGAATTCATAGAGAGTCTTGACAAGTTTGCCGGGCTGCAGCGCAGCTGGAT
AGTGAAGGTTGAAGAAGATACAAGCCCCGACTACGGCACGCCGCCGTGGGCTCGGCCGATAGAGAAGCATATCAGGATGG
GTGTTATACCCCTAGACAAGCCTCCGGGTCCCACGAGCCACGAGGTTGTAGCATGGATCAAGAGGATGTTCGGCTTATCC
AAGGCCGGGCATGGCGGCACGCTGGATCCGAAGGTTACCGGTGTACTCCCTGTAGCCCTTGAAGAGGGTACCAAGGTTAT
AGGACTAGTGGTCCACACCGGCAAAGAGTACATGTGTGTTATGCAGCTCCACCGTCCAGTCCCGGAGGAGGAGCTACGCA
GGGCAATCAACATGTTCGTAGGCGAGATCTATCAGAGGCCACCGCTGCGCAGCAGCGTCAAGAGAAGCCTGAGATTGAAG
AAGATCTATGAGATAGAGCTGCTGGAGTATAATGGCCGCTATGCCCTCATGAGGGTGTTCTCCGAGGCTGGCACCTACAT
GAGAAAGCTCTGCCACGACATAGGCCTAATACTCGGCGTCGGCGCGCACATGAGGGAGCTGCGTAGAACCAGGAGTGGCC
CATTCCGCGAGCGTAACGGGCTTGTAAGGCTGCAAGAGCTAAGCGAGGCAATCTACCGCTGGAAGCAGGAAGGCAAGGAG
GACCTCCTCCGCAAGTACATACTCCCAATAGAGTATGCAGTTAGCCACCTGCCAAAGATAGTGGTTAGGGACACGGCAGT
CGACGCAATAGCGCATGGTGCTAACCTCGCGGTACCGGGTATTGCTAGGCTACACGAGGGCATCAAGCGCGGAGACCTCG
TAGCACTACTAACACTAAAGGGCGAACTCGTAGCAATAGGCGTAGCCCAGATGTCCAGCGAAGAGATAGCAAAGGCCAAG
AAGGGCATAGCAGTGAAGATAAGGAGGGTCATAATGCCGCCAGGCGTCTACCCGCGCGCCTGGAAGAAAAAGAAGTAG

Upstream 100 bases:

>100_bases
GCCTGGCCTCCTCTAGCACCATACTGCCAAGCAGGTCATATCCCCGGCCCCAGATACTATACCTGGTTCTTTTCGGCCCG
GCTAGTATCGGGTGGGCATG

Downstream 100 bases:

>100_bases
GGTCAAACCCTGAGGATTCTCCTCCGGGTGCTAACAGCCCGTGGCAGCCCACTACTACAGACCACGGAGCCGTAAAGGGC
CGCGAATGCTAGTCAGCGAC

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSSELTRKGLEFIESLDKFAGLQRSWIVKVEEDTSPDYGTPPWARPIEKHIRMGVIPLDKPPGPTSHEVVAWIKRMFGLS
KAGHGGTLDPKVTGVLPVALEEGTKVIGLVVHTGKEYMCVMQLHRPVPEEELRRAINMFVGEIYQRPPLRSSVKRSLRLK
KIYEIELLEYNGRYALMRVFSEAGTYMRKLCHDIGLILGVGAHMRELRRTRSGPFRERNGLVRLQELSEAIYRWKQEGKE
DLLRKYILPIEYAVSHLPKIVVRDTAVDAIAHGANLAVPGIARLHEGIKRGDLVALLTLKGELVAIGVAQMSSEEIAKAK
KGIAVKIRRVIMPPGVYPRAWKKKK

Sequences:

>Translated_345_residues
MSSELTRKGLEFIESLDKFAGLQRSWIVKVEEDTSPDYGTPPWARPIEKHIRMGVIPLDKPPGPTSHEVVAWIKRMFGLS
KAGHGGTLDPKVTGVLPVALEEGTKVIGLVVHTGKEYMCVMQLHRPVPEEELRRAINMFVGEIYQRPPLRSSVKRSLRLK
KIYEIELLEYNGRYALMRVFSEAGTYMRKLCHDIGLILGVGAHMRELRRTRSGPFRERNGLVRLQELSEAIYRWKQEGKE
DLLRKYILPIEYAVSHLPKIVVRDTAVDAIAHGANLAVPGIARLHEGIKRGDLVALLTLKGELVAIGVAQMSSEEIAKAK
KGIAVKIRRVIMPPGVYPRAWKKKK
>Mature_344_residues
SSELTRKGLEFIESLDKFAGLQRSWIVKVEEDTSPDYGTPPWARPIEKHIRMGVIPLDKPPGPTSHEVVAWIKRMFGLSK
AGHGGTLDPKVTGVLPVALEEGTKVIGLVVHTGKEYMCVMQLHRPVPEEELRRAINMFVGEIYQRPPLRSSVKRSLRLKK
IYEIELLEYNGRYALMRVFSEAGTYMRKLCHDIGLILGVGAHMRELRRTRSGPFRERNGLVRLQELSEAIYRWKQEGKED
LLRKYILPIEYAVSHLPKIVVRDTAVDAIAHGANLAVPGIARLHEGIKRGDLVALLTLKGELVAIGVAQMSSEEIAKAKK
GIAVKIRRVIMPPGVYPRAWKKKK

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI4503337, Length=306, Percent_Identity=41.5032679738562, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI215599015, Length=306, Percent_Identity=41.5032679738562, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI21040257, Length=214, Percent_Identity=28.9719626168224, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI2367200, Length=276, Percent_Identity=28.2608695652174, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17553978, Length=306, Percent_Identity=38.8888888888889, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6323204, Length=306, Percent_Identity=43.7908496732026, Blast_Score=278, Evalue=1e-75,
Organism=Drosophila melanogaster, GI281364189, Length=307, Percent_Identity=39.0879478827362, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI281364187, Length=307, Percent_Identity=39.0879478827362, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI281364185, Length=307, Percent_Identity=39.0879478827362, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI281364183, Length=307, Percent_Identity=39.0879478827362, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI62471759, Length=307, Percent_Identity=39.0879478827362, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI17975520, Length=307, Percent_Identity=39.0879478827362, Blast_Score=254, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 38884; Mature: 38753

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSELTRKGLEFIESLDKFAGLQRSWIVKVEEDTSPDYGTPPWARPIEKHIRMGVIPLDK
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCEEECCC
PPGPTSHEVVAWIKRMFGLSKAGHGGTLDPKVTGVLPVALEEGTKVIGLVVHTGKEYMCV
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEHHCCCEEEEEEEECCCHHHHH
MQLHRPVPEEELRRAINMFVGEIYQRPPLRSSVKRSLRLKKIYEIELLEYNGRYALMRVF
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCHHHHHHH
SEAGTYMRKLCHDIGLILGVGAHMRELRRTRSGPFRERNGLVRLQELSEAIYRWKQEGKE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCHH
DLLRKYILPIEYAVSHLPKIVVRDTAVDAIAHGANLAVPGIARLHEGIKRGDLVALLTLK
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEEEEC
GELVAIGVAQMSSEEIAKAKKGIAVKIRRVIMPPGVYPRAWKKKK
CCEEEEEHHHHCHHHHHHHHCCHHEEEHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSELTRKGLEFIESLDKFAGLQRSWIVKVEEDTSPDYGTPPWARPIEKHIRMGVIPLDK
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCEEECCC
PPGPTSHEVVAWIKRMFGLSKAGHGGTLDPKVTGVLPVALEEGTKVIGLVVHTGKEYMCV
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEHHCCCEEEEEEEECCCHHHHH
MQLHRPVPEEELRRAINMFVGEIYQRPPLRSSVKRSLRLKKIYEIELLEYNGRYALMRVF
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCHHHHHHH
SEAGTYMRKLCHDIGLILGVGAHMRELRRTRSGPFRERNGLVRLQELSEAIYRWKQEGKE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCHH
DLLRKYILPIEYAVSHLPKIVVRDTAVDAIAHGANLAVPGIARLHEGIKRGDLVALLTLK
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEEEEC
GELVAIGVAQMSSEEIAKAKKGIAVKIRRVIMPPGVYPRAWKKKK
CCEEEEEHHHHCHHHHHHHHCCHHEEEHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]