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The map label for this gene is glyA

Identifier: 124028109

GI number: 124028109

Start: 1258994

End: 1260310

Strand: Reverse

Name: glyA

Synonym: Hbut_1252

Alternate gene names: 124028109

Gene position: 1260310-1258994 (Counterclockwise)

Preceding gene: 124028110

Following gene: 124028108

Centisome position: 75.6

GC content: 51.48

Gene sequence:

>1317_bases
GTGCTCCGGTTCTGGCAGGAAAAGCTACCCCTTCTCCCCCAGGAGCTGAGGGAGGTTCTCGAAAAGACGATAACACACAA
TCTATGGCGCAAGTACGAGACTATAAATCTTATAGCCAGCGAGAACGCTATGAGCCCTCTCGCGCTAGCAGCATACGTTA
GCGACATGATGCACCGGTATGCTGAGGGTAAGCCATTCAAGAGATACTACCAGGGCACTCGATATGTCGACGAGATTGAA
CATAGAGTTATGCAGCTTATGGGCGAGCTGCTCGGCGGAGCACATGTGGATCCCCGGCCCGTTAGTGGGACTACTGCAAA
CGCATCAGCTTTTAGAGCGCTAACCAACTGCGGCGATAAGGCGGTTGTTGCACCAGTACAGGCAGGAGCACATGTTAGTC
ATACAAAGTTTGGTACACTTGGCGGACTTTGCATAGAGCATATCGAGATGCCCTACGACCCGGAGAACATGAACATCGAC
GTTGACAAGGCTATCCGACTAATAGAGGAGGTTAGGCCACGCCTCGTTGTGCTCGGTGGTAGTGTCTATCTATTCCCCCA
TCCGGTAAAGGAGATCGCTGATACAGCTCACTCCGTTGGTGCAAAGCTAGTGTATGACGCTGCTCACGTACTAGGCCTCA
TAGTCGGGAGGAGGTGGAGAAACCCGCTAGACCATGGAGCAGATGTTATGACAGCCTCAACACACAAGACCTTCCCCGGG
CCCCAGGGCGGGATAGTAGCTACACGGAGCGAGGAACTTTACAAGACCATATCAAGGGTTGTCTTCCCCGTATTTGTCAG
CAACCACCATCTACATAGGCTCCCCGCACTCGCCGTTACCGCTGTAGAAATGAAGTACTTTGGCGAACAATACGCCGACC
AGGTTGTTAGGAATGCTAAGGCACTAGCAGAAGCTCTCGCAGCTGAGGGCTTCAAGGTTCTAGGAGAGCATCTAGGCTAC
ACTAAGAGCCACCAAGTACTAGTTGATGTAAGGGCCCAGGGTGGCGGTGCAAAAGCAGCTACTCTACTTGAGAAGGCAAA
CATAATTGTAAACAAGAACCTACTACCCTACGATCCCCCAGACGCTATCAAGGATCCAAGTGGCCTACGTCTAGGAGTAC
AAGAGATGACCAGGTACGGCATGAAGGAGGACAATATGAAGGATATAGCAAGGTTCATGAGAAGAGTGCTTATCGACGGC
GAGGACCCCGAGAAGGTGGCGAGAGAAGTAAAGGAGTACCGTAAAGAATACCTTGAGGTCAAATACTGCTTCGATGTAAA
CCCACTCGAGGATGGAAGGCTCTACCTCCTACTCTAG

Upstream 100 bases:

>100_bases
AGCGAGTATCAGTAAAGCCCCTATACTAGCATGATCACTGCTTCAAGGAGCTTGCGTTAATTAGTCTCTACTATATCACC
GGCTACCCGGTGCCAGGAAA

Downstream 100 bases:

>100_bases
TTCCCAGCTCAACTCCATCTTTTCCACGTACCGAGAGAATAGGTTGCGGGAGGCAAGTCCCCTTACCCTCATCCAGCCCC
GTGCCGCGGGGCCGTCCGGG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLRFWQEKLPLLPQELREVLEKTITHNLWRKYETINLIASENAMSPLALAAYVSDMMHRYAEGKPFKRYYQGTRYVDEIE
HRVMQLMGELLGGAHVDPRPVSGTTANASAFRALTNCGDKAVVAPVQAGAHVSHTKFGTLGGLCIEHIEMPYDPENMNID
VDKAIRLIEEVRPRLVVLGGSVYLFPHPVKEIADTAHSVGAKLVYDAAHVLGLIVGRRWRNPLDHGADVMTASTHKTFPG
PQGGIVATRSEELYKTISRVVFPVFVSNHHLHRLPALAVTAVEMKYFGEQYADQVVRNAKALAEALAAEGFKVLGEHLGY
TKSHQVLVDVRAQGGGAKAATLLEKANIIVNKNLLPYDPPDAIKDPSGLRLGVQEMTRYGMKEDNMKDIARFMRRVLIDG
EDPEKVAREVKEYRKEYLEVKYCFDVNPLEDGRLYLLL

Sequences:

>Translated_438_residues
MLRFWQEKLPLLPQELREVLEKTITHNLWRKYETINLIASENAMSPLALAAYVSDMMHRYAEGKPFKRYYQGTRYVDEIE
HRVMQLMGELLGGAHVDPRPVSGTTANASAFRALTNCGDKAVVAPVQAGAHVSHTKFGTLGGLCIEHIEMPYDPENMNID
VDKAIRLIEEVRPRLVVLGGSVYLFPHPVKEIADTAHSVGAKLVYDAAHVLGLIVGRRWRNPLDHGADVMTASTHKTFPG
PQGGIVATRSEELYKTISRVVFPVFVSNHHLHRLPALAVTAVEMKYFGEQYADQVVRNAKALAEALAAEGFKVLGEHLGY
TKSHQVLVDVRAQGGGAKAATLLEKANIIVNKNLLPYDPPDAIKDPSGLRLGVQEMTRYGMKEDNMKDIARFMRRVLIDG
EDPEKVAREVKEYRKEYLEVKYCFDVNPLEDGRLYLLL
>Mature_438_residues
MLRFWQEKLPLLPQELREVLEKTITHNLWRKYETINLIASENAMSPLALAAYVSDMMHRYAEGKPFKRYYQGTRYVDEIE
HRVMQLMGELLGGAHVDPRPVSGTTANASAFRALTNCGDKAVVAPVQAGAHVSHTKFGTLGGLCIEHIEMPYDPENMNID
VDKAIRLIEEVRPRLVVLGGSVYLFPHPVKEIADTAHSVGAKLVYDAAHVLGLIVGRRWRNPLDHGADVMTASTHKTFPG
PQGGIVATRSEELYKTISRVVFPVFVSNHHLHRLPALAVTAVEMKYFGEQYADQVVRNAKALAEALAAEGFKVLGEHLGY
TKSHQVLVDVRAQGGGAKAATLLEKANIIVNKNLLPYDPPDAIKDPSGLRLGVQEMTRYGMKEDNMKDIARFMRRVLIDG
EDPEKVAREVKEYRKEYLEVKYCFDVNPLEDGRLYLLL

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=418, Percent_Identity=32.0574162679426, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI261862352, Length=418, Percent_Identity=32.0574162679426, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI261862350, Length=418, Percent_Identity=32.0574162679426, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI261862348, Length=418, Percent_Identity=32.0574162679426, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI261862346, Length=413, Percent_Identity=31.4769975786925, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI22547186, Length=461, Percent_Identity=29.5010845986985, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI22547189, Length=444, Percent_Identity=28.6036036036036, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788902, Length=389, Percent_Identity=36.2467866323907, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI25144729, Length=388, Percent_Identity=29.8969072164948, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25144732, Length=388, Percent_Identity=29.8969072164948, Blast_Score=155, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319739, Length=389, Percent_Identity=30.0771208226221, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6323087, Length=407, Percent_Identity=30.4668304668305, Blast_Score=156, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24640005, Length=441, Percent_Identity=31.5192743764172, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI221329721, Length=441, Percent_Identity=31.5192743764172, Blast_Score=175, Evalue=5e-44,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_HYPBU (A2BM73)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013429.1
- ProteinModelPortal:   A2BM73
- SMR:   A2BM73
- STRING:   A2BM73
- GeneID:   4781520
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1252
- eggNOG:   arNOG04649
- HOGENOM:   HBG301263
- OMA:   SSTHKTF
- ProtClustDB:   PRK00011
- BioCyc:   HBUT415426:HBUT_1252-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_A
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 49032; Mature: 49032

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFWQEKLPLLPQELREVLEKTITHNLWRKYETINLIASENAMSPLALAAYVSDMMHRY
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
AEGKPFKRYYQGTRYVDEIEHRVMQLMGELLGGAHVDPRPVSGTTANASAFRALTNCGDK
HCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
AVVAPVQAGAHVSHTKFGTLGGLCIEHIEMPYDPENMNIDVDKAIRLIEEVRPRLVVLGG
EEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECC
SVYLFPHPVKEIADTAHSVGAKLVYDAAHVLGLIVGRRWRNPLDHGADVMTASTHKTFPG
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEECCCCCCCCC
PQGGIVATRSEELYKTISRVVFPVFVSNHHLHRLPALAVTAVEMKYFGEQYADQVVRNAK
CCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAEALAAEGFKVLGEHLGYTKSHQVLVDVRAQGGGAKAATLLEKANIIVNKNLLPYDPP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEECCCCCCCCC
DAIKDPSGLRLGVQEMTRYGMKEDNMKDIARFMRRVLIDGEDPEKVAREVKEYRKEYLEV
HHCCCCCCCEECHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEE
KYCFDVNPLEDGRLYLLL
EEEEECCCCCCCCEEEEC
>Mature Secondary Structure
MLRFWQEKLPLLPQELREVLEKTITHNLWRKYETINLIASENAMSPLALAAYVSDMMHRY
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
AEGKPFKRYYQGTRYVDEIEHRVMQLMGELLGGAHVDPRPVSGTTANASAFRALTNCGDK
HCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
AVVAPVQAGAHVSHTKFGTLGGLCIEHIEMPYDPENMNIDVDKAIRLIEEVRPRLVVLGG
EEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECC
SVYLFPHPVKEIADTAHSVGAKLVYDAAHVLGLIVGRRWRNPLDHGADVMTASTHKTFPG
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEECCCCCCCCC
PQGGIVATRSEELYKTISRVVFPVFVSNHHLHRLPALAVTAVEMKYFGEQYADQVVRNAK
CCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAEALAAEGFKVLGEHLGYTKSHQVLVDVRAQGGGAKAATLLEKANIIVNKNLLPYDPP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEECCCCCCCCC
DAIKDPSGLRLGVQEMTRYGMKEDNMKDIARFMRRVLIDGEDPEKVAREVKEYRKEYLEV
HHCCCCCCCEECHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEE
KYCFDVNPLEDGRLYLLL
EEEEECCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA