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The map label for this gene is serS

Identifier: 124028106

GI number: 124028106

Start: 1255765

End: 1257147

Strand: Reverse

Name: serS

Synonym: Hbut_1249

Alternate gene names: 124028106

Gene position: 1257147-1255765 (Counterclockwise)

Preceding gene: 124028107

Following gene: 124028105

Centisome position: 75.41

GC content: 49.53

Gene sequence:

>1383_bases
TTGACTTGGAGCATATTGCGCTACTTGCGTGAAAACCCCGACATACTGAAGGATAGCATGAGAAAGAGAGGACTAGACCC
ATCAGTTATCGATGAGGCTAAGAGAGTCGACGAGGAGTGGAGGAAGCTTAAGAGGCAAGTTGACGAGATAAGACACCGTC
ATAACGTGATAACAAGACAGATAGCTTCAGCTAGGGATCCCGAGGAGCGGAGGAAACTGATAGAGGAGGCTAGAAGACTC
CTAGCTGAGAGGGAGGAGCTGGAAAAGAAGCTTAAGGCTCTCGAGGAGGAACGCGAGAGGATACTCCTATCTCTACCAAA
CATCGTGCATGACAGTGTACCGGTAGGCTTTTCAGACGAGGAGAACGTGCCCATTCGCTTCTGGGGCCGGCCAAGGGTTT
ACAGAGGGCATCTTGACGCGTTTAAAGCGCAAACCGAACGATGGGGTTTCAAGGTAGACTATGAGCTTATCGACTGGAAA
CCGGTAGGCCACGCTGACATGCTTGAATACGTGTTAAAGCTTGGCAATACTATGAAGGCTGCCGAAGTTGCAGGTTCACG
CTTCTACTACCTCTTCGACGACATTGTCTGGCTCGACTTTGCACTGCTCCTCTATGCTATAGACAGGCTTACCCAGAAGG
GCTACCGACTCGTACTACCACCATACATGATAAGGCACAAAATACTGATGGGCGTAATAGACATGGAGACCTTTAAGGAC
GCAATATACAAGGTTGAAGACGAAGACCTATACCTCATAGCTACCGCTGAACACCCTCTCGCTGGCCTTTACTACAACGA
GGAGATAATGGAGGACGAGCTACCCATAAAGCTTGTCGGTATAAGTCCATGCTTTCGCAAGGAGGCCGGTGCAGGTAACC
GCGACCTCAAAGGAATATTCCGTGTACACCAGTTCCACAAGGTCGAGCAGTTTGTGTACGCAAAACCAGAGGAGAGCTGG
GATATTCTTGAAGAGCTGATAAGGAATGCTGAGGAACTATTCCAGGGGCTAGGCCTACCATACCGTGTCGTAAACGTTGT
CTCCGGCGAGCTAGGCGCACCCGCAGCTAAGAAGTATGACCTTGAGGTGTGGATGCCTGCACAAGGCAAGTATAGGGAAA
TGGTTAGTGCAAGCAACACCACTGACTGGCAGAGCTACCGGCTAAATATAAGGCTCGTGAGGAAGAAAGATATGAAGCGC
GAGTACGTACACACACTAAACTCAACAGCCATAGCTTCAACAAGAACGATAACAGCCATACTGGAAAACTTCCAGGAGCC
CGACGGCACAGTAGTCATACCTAAGGTGCTGAGAAAATATCTTGAAATATTCCCGAAAGCACCAAAAGATGCTATCCACC
CCGTGAAGAAGGTGAGGGGCTAG

Upstream 100 bases:

>100_bases
CGATTAACACCATTTAACCGGGAGAGTAAGAGGAATAGATGCGTAGCAGCATCCGTAGCACAGCTGGAGCCCTCGGTAAG
CCTAGGGGTGTACTTGTATC

Downstream 100 bases:

>100_bases
AGAACAGTGGCTCTTACACGAGAGGAGCTAAAGGCACGGATACTAGATATTGTCGAAAACGCCCCACAGATGAACAAGGC
AGCATTCTACAGCGATCCCT

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTWSILRYLRENPDILKDSMRKRGLDPSVIDEAKRVDEEWRKLKRQVDEIRHRHNVITRQIASARDPEERRKLIEEARRL
LAEREELEKKLKALEEERERILLSLPNIVHDSVPVGFSDEENVPIRFWGRPRVYRGHLDAFKAQTERWGFKVDYELIDWK
PVGHADMLEYVLKLGNTMKAAEVAGSRFYYLFDDIVWLDFALLLYAIDRLTQKGYRLVLPPYMIRHKILMGVIDMETFKD
AIYKVEDEDLYLIATAEHPLAGLYYNEEIMEDELPIKLVGISPCFRKEAGAGNRDLKGIFRVHQFHKVEQFVYAKPEESW
DILEELIRNAEELFQGLGLPYRVVNVVSGELGAPAAKKYDLEVWMPAQGKYREMVSASNTTDWQSYRLNIRLVRKKDMKR
EYVHTLNSTAIASTRTITAILENFQEPDGTVVIPKVLRKYLEIFPKAPKDAIHPVKKVRG

Sequences:

>Translated_460_residues
MTWSILRYLRENPDILKDSMRKRGLDPSVIDEAKRVDEEWRKLKRQVDEIRHRHNVITRQIASARDPEERRKLIEEARRL
LAEREELEKKLKALEEERERILLSLPNIVHDSVPVGFSDEENVPIRFWGRPRVYRGHLDAFKAQTERWGFKVDYELIDWK
PVGHADMLEYVLKLGNTMKAAEVAGSRFYYLFDDIVWLDFALLLYAIDRLTQKGYRLVLPPYMIRHKILMGVIDMETFKD
AIYKVEDEDLYLIATAEHPLAGLYYNEEIMEDELPIKLVGISPCFRKEAGAGNRDLKGIFRVHQFHKVEQFVYAKPEESW
DILEELIRNAEELFQGLGLPYRVVNVVSGELGAPAAKKYDLEVWMPAQGKYREMVSASNTTDWQSYRLNIRLVRKKDMKR
EYVHTLNSTAIASTRTITAILENFQEPDGTVVIPKVLRKYLEIFPKAPKDAIHPVKKVRG
>Mature_459_residues
TWSILRYLRENPDILKDSMRKRGLDPSVIDEAKRVDEEWRKLKRQVDEIRHRHNVITRQIASARDPEERRKLIEEARRLL
AEREELEKKLKALEEERERILLSLPNIVHDSVPVGFSDEENVPIRFWGRPRVYRGHLDAFKAQTERWGFKVDYELIDWKP
VGHADMLEYVLKLGNTMKAAEVAGSRFYYLFDDIVWLDFALLLYAIDRLTQKGYRLVLPPYMIRHKILMGVIDMETFKDA
IYKVEDEDLYLIATAEHPLAGLYYNEEIMEDELPIKLVGISPCFRKEAGAGNRDLKGIFRVHQFHKVEQFVYAKPEESWD
ILEELIRNAEELFQGLGLPYRVVNVVSGELGAPAAKKYDLEVWMPAQGKYREMVSASNTTDWQSYRLNIRLVRKKDMKRE
YVHTLNSTAIASTRTITAILENFQEPDGTVVIPKVLRKYLEIFPKAPKDAIHPVKKVRG

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI16306548, Length=473, Percent_Identity=34.8837209302326, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI8923421, Length=419, Percent_Identity=30.3102625298329, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI225579074, Length=354, Percent_Identity=32.4858757062147, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI1787120, Length=444, Percent_Identity=34.4594594594595, Blast_Score=253, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17539260, Length=489, Percent_Identity=35.3783231083845, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17542084, Length=356, Percent_Identity=32.8651685393258, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6320226, Length=439, Percent_Identity=36.4464692482916, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321799, Length=396, Percent_Identity=36.6161616161616, Blast_Score=229, Evalue=8e-61,
Organism=Drosophila melanogaster, GI24581334, Length=489, Percent_Identity=33.7423312883436, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI17737985, Length=260, Percent_Identity=38.4615384615385, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_HYPBU (A2BM70)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013426.1
- ProteinModelPortal:   A2BM70
- SMR:   A2BM70
- STRING:   A2BM70
- GeneID:   4782367
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1249
- eggNOG:   arNOG04439
- HOGENOM:   HBG629391
- OMA:   VPIRFWG
- PhylomeDB:   A2BM70
- ProtClustDB:   PRK05431
- BioCyc:   HBUT415426:HBUT_1249-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 53882; Mature: 53751

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 302-302 BINDING 309-309 BINDING 409-409

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWSILRYLRENPDILKDSMRKRGLDPSVIDEAKRVDEEWRKLKRQVDEIRHRHNVITRQ
CCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IASARDPEERRKLIEEARRLLAEREELEKKLKALEEERERILLSLPNIVHDSVPVGFSDE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCC
ENVPIRFWGRPRVYRGHLDAFKAQTERWGFKVDYELIDWKPVGHADMLEYVLKLGNTMKA
CCCCEEECCCCCEEECHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCCHHH
AEVAGSRFYYLFDDIVWLDFALLLYAIDRLTQKGYRLVLPPYMIRHKILMGVIDMETFKD
HHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHH
AIYKVEDEDLYLIATAEHPLAGLYYNEEIMEDELPIKLVGISPCFRKEAGAGNRDLKGIF
HHHEECCCCEEEEEECCCCCEEEEECHHHHCCCCCEEEEECCHHHHHHCCCCCCHHHHHH
RVHQFHKVEQFVYAKPEESWDILEELIRNAEELFQGLGLPYRVVNVVSGELGAPAAKKYD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCC
LEVWMPAQGKYREMVSASNTTDWQSYRLNIRLVRKKDMKREYVHTLNSTAIASTRTITAI
EEEEECCCCHHHHHHHCCCCCCCHHEEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHH
LENFQEPDGTVVIPKVLRKYLEIFPKAPKDAIHPVKKVRG
HHHHCCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TWSILRYLRENPDILKDSMRKRGLDPSVIDEAKRVDEEWRKLKRQVDEIRHRHNVITRQ
CHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IASARDPEERRKLIEEARRLLAEREELEKKLKALEEERERILLSLPNIVHDSVPVGFSDE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCC
ENVPIRFWGRPRVYRGHLDAFKAQTERWGFKVDYELIDWKPVGHADMLEYVLKLGNTMKA
CCCCEEECCCCCEEECHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCCHHH
AEVAGSRFYYLFDDIVWLDFALLLYAIDRLTQKGYRLVLPPYMIRHKILMGVIDMETFKD
HHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHH
AIYKVEDEDLYLIATAEHPLAGLYYNEEIMEDELPIKLVGISPCFRKEAGAGNRDLKGIF
HHHEECCCCEEEEEECCCCCEEEEECHHHHCCCCCEEEEECCHHHHHHCCCCCCHHHHHH
RVHQFHKVEQFVYAKPEESWDILEELIRNAEELFQGLGLPYRVVNVVSGELGAPAAKKYD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCC
LEVWMPAQGKYREMVSASNTTDWQSYRLNIRLVRKKDMKREYVHTLNSTAIASTRTITAI
EEEEECCCCHHHHHHHCCCCCCCHHEEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHH
LENFQEPDGTVVIPKVLRKYLEIFPKAPKDAIHPVKKVRG
HHHHCCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA