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The map label for this gene is prf1 [H]

Identifier: 124028078

GI number: 124028078

Start: 1231206

End: 1232318

Strand: Reverse

Name: prf1 [H]

Synonym: Hbut_1221

Alternate gene names: 124028078

Gene position: 1232318-1231206 (Counterclockwise)

Preceding gene: 124028080

Following gene: 124028077

Centisome position: 73.92

GC content: 52.02

Gene sequence:

>1113_bases
ATGCAGCAAGCTAGCCAGCTAATGGTTGACAAGAGAACACTGAAACAGATTATTAGAGAGCTTAAGCGCTGGAAGGCGCC
GGCAACGGTACTGCTAACACTCTACATACCACCAGGCAGGCCCATAAGCGACGTTGTCAGCCTGCTTAGAACCGAGTACT
CGATAACCGACAATATTAAGCTCAAGAGGACCCGCGACGCTGTACAGAGGGCACTAGCAGCCGCCATGGATAGGCTTACG
AGGATACAGAAGGTGCCGCCTAACGGCTTAGCAGTCTTCTGCGGAGAGGACATGGAGACTAAGGACTTCATATGCTTCGT
CTTCTCGCCGCCAGAACCGGTGCCAGTCTTCTTCTACCGTACCGACAAGTGGTTCCATACAGAATTCCTCGAGGAAATGA
TAGAACAAGAGGAGACCTACGGCCTCGTAATAATCGAGCGCGACCAGGCCACTATAGGCCTGCTTAAGGGTACACGTATA
CAAGTTCTAGAGGAACTTGAGGGCTACGTGCCAGGCAAGCACCATAAGGGCGGTCAGAGCCAGAGGCGTTTCGATAGGCA
GATCGAGCAATTCGTAAAGGGCTTCTACCGCGAGGTAGCCGAAGCAATGAACAAACACTTCCTGCCACTCATAGAGAAGG
GTGTCCTCAGAGCGATAATACTCGGTGGCCCCGGCTACGCCAAGCAAGACTTCCTCGAGGAAGCAAGGGACTTCCTGGAC
TACCGTGTACGCCAAAAGATAGCAAGCAAGACCGTCGATGTAGCCTATCAGGGCGAGGCTGGGCTGAGAGAGCTAGTCAT
AAAGGCTGCCGACATAATTGAGCAGAACAAGTACGCAGAACTCGCTAAGGCTGTTGAGGAGTTTAAGCTCCACCTAGCAA
AGGATGACGGGCTAGCAGTATATGGCGACAAGATGGTAAAAACAGCCCTCGAAATGGGTGCTCTGAAATACATTATAGTC
GACGAGGAGAGGCCTGATGCAGATACACTGGCGGAGGAGGCTAAGAAGTATGGCACCAAGGTTTACTTCTTGAGCGGAGA
CCTACCTGAGGCGGAGTGGATACGCAAGACGTTCAAGGGGCTTGTTGGCGTGCTACACTTCAGGATAATGTAA

Upstream 100 bases:

>100_bases
GCAGAACCCCATAGTAATACTGTTTGGCCAGGATAGCTTGGCATGGTATACGTTATAAGGTGTCGCTCTGTTAGTTTATG
TGTGCACAGGGGAAGGTGGA

Downstream 100 bases:

>100_bases
TGCACAGCAGAGAACGATACTGGAGTGAGTATGCAGCCTCTGGCGCTACTGTGGCTCGATGGTTGCTGGCGGCTTGGTTG
TCACCGCATACGCAACCATT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MQQASQLMVDKRTLKQIIRELKRWKAPATVLLTLYIPPGRPISDVVSLLRTEYSITDNIKLKRTRDAVQRALAAAMDRLT
RIQKVPPNGLAVFCGEDMETKDFICFVFSPPEPVPVFFYRTDKWFHTEFLEEMIEQEETYGLVIIERDQATIGLLKGTRI
QVLEELEGYVPGKHHKGGQSQRRFDRQIEQFVKGFYREVAEAMNKHFLPLIEKGVLRAIILGGPGYAKQDFLEEARDFLD
YRVRQKIASKTVDVAYQGEAGLRELVIKAADIIEQNKYAELAKAVEEFKLHLAKDDGLAVYGDKMVKTALEMGALKYIIV
DEERPDADTLAEEAKKYGTKVYFLSGDLPEAEWIRKTFKGLVGVLHFRIM

Sequences:

>Translated_370_residues
MQQASQLMVDKRTLKQIIRELKRWKAPATVLLTLYIPPGRPISDVVSLLRTEYSITDNIKLKRTRDAVQRALAAAMDRLT
RIQKVPPNGLAVFCGEDMETKDFICFVFSPPEPVPVFFYRTDKWFHTEFLEEMIEQEETYGLVIIERDQATIGLLKGTRI
QVLEELEGYVPGKHHKGGQSQRRFDRQIEQFVKGFYREVAEAMNKHFLPLIEKGVLRAIILGGPGYAKQDFLEEARDFLD
YRVRQKIASKTVDVAYQGEAGLRELVIKAADIIEQNKYAELAKAVEEFKLHLAKDDGLAVYGDKMVKTALEMGALKYIIV
DEERPDADTLAEEAKKYGTKVYFLSGDLPEAEWIRKTFKGLVGVLHFRIM
>Mature_370_residues
MQQASQLMVDKRTLKQIIRELKRWKAPATVLLTLYIPPGRPISDVVSLLRTEYSITDNIKLKRTRDAVQRALAAAMDRLT
RIQKVPPNGLAVFCGEDMETKDFICFVFSPPEPVPVFFYRTDKWFHTEFLEEMIEQEETYGLVIIERDQATIGLLKGTRI
QVLEELEGYVPGKHHKGGQSQRRFDRQIEQFVKGFYREVAEAMNKHFLPLIEKGVLRAIILGGPGYAKQDFLEEARDFLD
YRVRQKIASKTVDVAYQGEAGLRELVIKAADIIEQNKYAELAKAVEEFKLHLAKDDGLAVYGDKMVKTALEMGALKYIIV
DEERPDADTLAEEAKKYGTKVYFLSGDLPEAEWIRKTFKGLVGVLHFRIM

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=315, Percent_Identity=30.4761904761905, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI72000368, Length=313, Percent_Identity=31.629392971246, Blast_Score=154, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319619, Length=378, Percent_Identity=27.7777777777778, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28574632, Length=315, Percent_Identity=30.1587301587302, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21356433, Length=315, Percent_Identity=30.1587301587302, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667392, Length=315, Percent_Identity=30.1587301587302, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667388, Length=315, Percent_Identity=30.1587301587302, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667380, Length=315, Percent_Identity=30.1587301587302, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667384, Length=315, Percent_Identity=30.1587301587302, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 42394; Mature: 42394

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQASQLMVDKRTLKQIIRELKRWKAPATVLLTLYIPPGRPISDVVSLLRTEYSITDNIK
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
LKRTRDAVQRALAAAMDRLTRIQKVPPNGLAVFCGEDMETKDFICFVFSPPEPVPVFFYR
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEEEE
TDKWFHTEFLEEMIEQEETYGLVIIERDQATIGLLKGTRIQVLEELEGYVPGKHHKGGQS
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCH
QRRFDRQIEQFVKGFYREVAEAMNKHFLPLIEKGVLRAIILGGPGYAKQDFLEEARDFLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHH
YRVRQKIASKTVDVAYQGEAGLRELVIKAADIIEQNKYAELAKAVEEFKLHLAKDDGLAV
HHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
YGDKMVKTALEMGALKYIIVDEERPDADTLAEEAKKYGTKVYFLSGDLPEAEWIRKTFKG
ECHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHH
LVGVLHFRIM
HHHHHHHHCC
>Mature Secondary Structure
MQQASQLMVDKRTLKQIIRELKRWKAPATVLLTLYIPPGRPISDVVSLLRTEYSITDNIK
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
LKRTRDAVQRALAAAMDRLTRIQKVPPNGLAVFCGEDMETKDFICFVFSPPEPVPVFFYR
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEEEE
TDKWFHTEFLEEMIEQEETYGLVIIERDQATIGLLKGTRIQVLEELEGYVPGKHHKGGQS
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCH
QRRFDRQIEQFVKGFYREVAEAMNKHFLPLIEKGVLRAIILGGPGYAKQDFLEEARDFLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHH
YRVRQKIASKTVDVAYQGEAGLRELVIKAADIIEQNKYAELAKAVEEFKLHLAKDDGLAV
HHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
YGDKMVKTALEMGALKYIIVDEERPDADTLAEEAKKYGTKVYFLSGDLPEAEWIRKTFKG
ECHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHH
LVGVLHFRIM
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]