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The map label for this gene is alaXM [H]

Identifier: 124028073

GI number: 124028073

Start: 1222616

End: 1223341

Strand: Reverse

Name: alaXM [H]

Synonym: Hbut_1214

Alternate gene names: 124028073

Gene position: 1223341-1222616 (Counterclockwise)

Preceding gene: 124028074

Following gene: 124028070

Centisome position: 73.38

GC content: 48.48

Gene sequence:

>726_bases
TTGCCAGCAAAGCTTCTCTTTCAAGAGGATAGCTACCTCAAAGAGTTTGATGCCATAGTGACCCGTGTAGAAGGAAATGC
AGTATTTCTCGAGGCTACAGCATTTCATCCCAGGCCCTCAGGAGGCCTTGACGCTGATCATGGCATTCTAATACTACCTG
ACGGCTCAGAGCTGCGTGTAGTAGATGTCGTAATGCGTGAAGGCGATGTAGCACATATTGTTGATGGTGACTTGTCCAAG
CTGCATCCGGGAATGCGTGTCCATGGCGTTATTGACTGGGAGCGTAGGTACCAGATGATGAAGCTGCACACGGCCAGCCA
TATACTACTAGCAGTACTCTACAACAAGTACGGTGCAAGAGTTACTGGTGGCCATATAACGCCGGAGACGGCTAGGGATG
ATCTCGAGATAAATGTTGATGATTGGAAGTCTGCTGTCCGTCAAGCAGTAGAGGAGGCTAACCAGATCATTAGAAGGTGT
ATTGAGGTTAAAGTCTATTGGTTGCCGAGGGAGGAAGCCCTACGCATACCCGGGATAGTCAAACTGGCTGATAAACTTCC
ACCAGATGTTGAGAAGCTGAGAATAGTCGAAATACCTGGCGTAGATATACAAGCTGATGGCGGTCCACACGTGAGGAATA
CTTGTGAGATACCTGGGATAAAGATTGTGAAGCTGGAAAGCCGGGGTAGGAGGAGGAAGAGGGTCTACTACACATTGTTA
GAGTAG

Upstream 100 bases:

>100_bases
ATCGGCTGCAACTGGTAGCATGGTCTAATCCTAAAGCTAAACAGCTGATAATGAGTCTTTTTTACCCTGAGTCCAGGTCC
ACACTATGGGTGTCCCCGTT

Downstream 100 bases:

>100_bases
CCAGCTTCACGGCTCAGTTTCTATCCCTCCTTATAGGTAGGCGAGGACTATTGCAAGGACCAGCATTGCTAGCCATGCTA
TGAGATTTCTCCTCATTATC

Product: metal-dependent hydrolase

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MPAKLLFQEDSYLKEFDAIVTRVEGNAVFLEATAFHPRPSGGLDADHGILILPDGSELRVVDVVMREGDVAHIVDGDLSK
LHPGMRVHGVIDWERRYQMMKLHTASHILLAVLYNKYGARVTGGHITPETARDDLEINVDDWKSAVRQAVEEANQIIRRC
IEVKVYWLPREEALRIPGIVKLADKLPPDVEKLRIVEIPGVDIQADGGPHVRNTCEIPGIKIVKLESRGRRRKRVYYTLL
E

Sequences:

>Translated_241_residues
MPAKLLFQEDSYLKEFDAIVTRVEGNAVFLEATAFHPRPSGGLDADHGILILPDGSELRVVDVVMREGDVAHIVDGDLSK
LHPGMRVHGVIDWERRYQMMKLHTASHILLAVLYNKYGARVTGGHITPETARDDLEINVDDWKSAVRQAVEEANQIIRRC
IEVKVYWLPREEALRIPGIVKLADKLPPDVEKLRIVEIPGVDIQADGGPHVRNTCEIPGIKIVKLESRGRRRKRVYYTLL
E
>Mature_240_residues
PAKLLFQEDSYLKEFDAIVTRVEGNAVFLEATAFHPRPSGGLDADHGILILPDGSELRVVDVVMREGDVAHIVDGDLSKL
HPGMRVHGVIDWERRYQMMKLHTASHILLAVLYNKYGARVTGGHITPETARDDLEINVDDWKSAVRQAVEEANQIIRRCI
EVKVYWLPREEALRIPGIVKLADKLPPDVEKLRIVEIPGVDIQADGGPHVRNTCEIPGIKIVKLESRGRRRKRVYYTLLE

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI217416402, Length=218, Percent_Identity=27.0642201834862, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI13376886, Length=218, Percent_Identity=27.0642201834862, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 27234; Mature: 27103

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKLLFQEDSYLKEFDAIVTRVEGNAVFLEATAFHPRPSGGLDADHGILILPDGSELRV
CCCCEEECCCHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEE
VDVVMREGDVAHIVDGDLSKLHPGMRVHGVIDWERRYQMMKLHTASHILLAVLYNKYGAR
EEEEECCCCEEEEECCCHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VTGGHITPETARDDLEINVDDWKSAVRQAVEEANQIIRRCIEVKVYWLPREEALRIPGIV
EECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHCCCCCCCE
KLADKLPPDVEKLRIVEIPGVDIQADGGPHVRNTCEIPGIKIVKLESRGRRRKRVYYTLL
EHHHHCCCCHHHEEEEECCCCEEECCCCCCCCCEECCCCEEEEEECCCCCCCEEEEEEEE
E
C
>Mature Secondary Structure 
PAKLLFQEDSYLKEFDAIVTRVEGNAVFLEATAFHPRPSGGLDADHGILILPDGSELRV
CCCEEECCCHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEE
VDVVMREGDVAHIVDGDLSKLHPGMRVHGVIDWERRYQMMKLHTASHILLAVLYNKYGAR
EEEEECCCCEEEEECCCHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VTGGHITPETARDDLEINVDDWKSAVRQAVEEANQIIRRCIEVKVYWLPREEALRIPGIV
EECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHCCCCCCCE
KLADKLPPDVEKLRIVEIPGVDIQADGGPHVRNTCEIPGIKIVKLESRGRRRKRVYYTLL
EHHHHCCCCHHHEEEEECCCCEEECCCCCCCCCEECCCCEEEEEECCCCCCCEEEEEEEE
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]