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The map label for this gene is pycA [H]

Identifier: 124028019

GI number: 124028019

Start: 1168201

End: 1169676

Strand: Reverse

Name: pycA [H]

Synonym: Hbut_1157

Alternate gene names: 124028019

Gene position: 1169676-1168201 (Counterclockwise)

Preceding gene: 124028026

Following gene: 124028018

Centisome position: 70.16

GC content: 60.3

Gene sequence:

>1476_bases
GTGGCCCTGAGGGTGTTGGTAGCTACGCGTGGCGAGATAGCTATCCGCATCGCTCGCGCCGTTAGGGAGCTGGGCTGGGA
GCCTATAACCATATACGCCCCCGACGACGCCCATAGCCCCCATGTTAGGGCTGGCACGTTCTCCGTAATGGTTGAGAGCT
ATACTAACCCCGACTCCGTCGTGGAGGCGGCCATCAAGGCTGGCGCCGACATACTTCACCCCGGCTACGGCTTCCTCTCG
GAAGACCCCTCCTTTGCCAGGAAGGTGCTCGACGCCGGGATATCCTGGGCCGGTCCAAAGCCGGAGACCATGGAGCTCCT
AGGCGATAAGCACCGCGCCAAGCAGCTAGCAGAGAAGGTTGGCGTGCCCACGCCTCCCTGGTGCGAAGCTCGTAGTGCTG
AGGAGGCTGTGAGGTGTGCCGAGAGGATAGGATATCCGGTGATGTTGAAGGCTTCAAAGGCTGGCGGCGGTAGGGGCATG
AGGGTTGCGTCGAATAGCGAGGAGGTTAGGAGAGCTTACCGCCTCGTGAGCCTTGAGGCCCGCAATGGTTTCGGCGATGC
CGGAGTGGTCTACATAGAGAAGTATGTTAGGAACCCGAGGCACATCGAGGTGCAGATTCTCGGCGATGGGCAGGGCCACG
TGGTACACCTCTACGAGAGGGAGTGCTCCATCCAGAGGAGGAGGCAGAAGATTATAGAGGAGGCTCCGAGCCCGTTCGCC
GAGAAGGTGCCGCAGGTTAGAAGCAGACTCGTAGACTACGCTCTAAGGCTCGCCGAGGAGGCCGAGTACCAGAGCGCCGG
CACGATAGAGTTTATAGTTGATGAGAGGGGACAGCCATACTTCATAGAGGCTAATGCGAGGCTCCAGGTGGAGCACGGTG
TCACAGAGGCGGTGACAGGCATCGATATTGTAAAGCAGCAGCTCCTCATAGCTGCTGGCCGCGAGCTCCAGCTCGGACAA
GAGGACATCAAGCTCCACGGCTGGGCGATAGAAGCCCGCATCTACGCCGAGGACCCGGAGAACGGGTTCAGAGCCGTGGA
GGGCGTCGTAACCGGGTACCGTGAGCCCCGCGGCCCCGGCGTGAGGGTCGACTCTGGCGCCGAGAGGGGGCTAAGGATCT
CTACCCGCTACGACACACTGCTCCTCAAGGTTATCGCCTGGGGTAGGGATCGTGCTGAGGCTGTTGCAAGGCTACGTGCG
GCTCTCCACGAAACCGTTGTCGCAGGCGTAAAGACTAATCTCGAACTCCTCAGAACTATCGTGGAGTCTGACTGGTTTAG
GAAAGCAGCGTTTCACACGAGGATCCTCGAGGAGAAGCTGGGAGAGCTGCTCTCCCAGCTAAGGGCTAGGAGGAACATAG
TTAGGGCTATAGCCTCCAGGATACGGGCTAAGCCGCTCGGCCTAACGGGGATAGCTGCTGCCAAGCTACCGGTGGTAGCT
AGCCATGGATGGCCATGGCCGCCGTGGAGGACCTAG

Upstream 100 bases:

>100_bases
ACCTATCTCGCCACCTGTCAAAAACCCTTACTTATTTATCCGCTATCCACACGTTCCGAACGTGTTGGGCCCGCTAAGAG
GGTGATGGGTGTAGAGCACC

Downstream 100 bases:

>100_bases
GCTCGTCGAGTTCCGGGAGGTAGATATAGGCGTCTATGAGGCCGTTGTCGAGCTAGAGGACGGGCGCAAGGTTTCGGTTA
AGGCCCGCCTAGTAGGGGAC

Product: pyruvate carboxylase subunit A

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MALRVLVATRGEIAIRIARAVRELGWEPITIYAPDDAHSPHVRAGTFSVMVESYTNPDSVVEAAIKAGADILHPGYGFLS
EDPSFARKVLDAGISWAGPKPETMELLGDKHRAKQLAEKVGVPTPPWCEARSAEEAVRCAERIGYPVMLKASKAGGGRGM
RVASNSEEVRRAYRLVSLEARNGFGDAGVVYIEKYVRNPRHIEVQILGDGQGHVVHLYERECSIQRRRQKIIEEAPSPFA
EKVPQVRSRLVDYALRLAEEAEYQSAGTIEFIVDERGQPYFIEANARLQVEHGVTEAVTGIDIVKQQLLIAAGRELQLGQ
EDIKLHGWAIEARIYAEDPENGFRAVEGVVTGYREPRGPGVRVDSGAERGLRISTRYDTLLLKVIAWGRDRAEAVARLRA
ALHETVVAGVKTNLELLRTIVESDWFRKAAFHTRILEEKLGELLSQLRARRNIVRAIASRIRAKPLGLTGIAAAKLPVVA
SHGWPWPPWRT

Sequences:

>Translated_491_residues
MALRVLVATRGEIAIRIARAVRELGWEPITIYAPDDAHSPHVRAGTFSVMVESYTNPDSVVEAAIKAGADILHPGYGFLS
EDPSFARKVLDAGISWAGPKPETMELLGDKHRAKQLAEKVGVPTPPWCEARSAEEAVRCAERIGYPVMLKASKAGGGRGM
RVASNSEEVRRAYRLVSLEARNGFGDAGVVYIEKYVRNPRHIEVQILGDGQGHVVHLYERECSIQRRRQKIIEEAPSPFA
EKVPQVRSRLVDYALRLAEEAEYQSAGTIEFIVDERGQPYFIEANARLQVEHGVTEAVTGIDIVKQQLLIAAGRELQLGQ
EDIKLHGWAIEARIYAEDPENGFRAVEGVVTGYREPRGPGVRVDSGAERGLRISTRYDTLLLKVIAWGRDRAEAVARLRA
ALHETVVAGVKTNLELLRTIVESDWFRKAAFHTRILEEKLGELLSQLRARRNIVRAIASRIRAKPLGLTGIAAAKLPVVA
SHGWPWPPWRT
>Mature_490_residues
ALRVLVATRGEIAIRIARAVRELGWEPITIYAPDDAHSPHVRAGTFSVMVESYTNPDSVVEAAIKAGADILHPGYGFLSE
DPSFARKVLDAGISWAGPKPETMELLGDKHRAKQLAEKVGVPTPPWCEARSAEEAVRCAERIGYPVMLKASKAGGGRGMR
VASNSEEVRRAYRLVSLEARNGFGDAGVVYIEKYVRNPRHIEVQILGDGQGHVVHLYERECSIQRRRQKIIEEAPSPFAE
KVPQVRSRLVDYALRLAEEAEYQSAGTIEFIVDERGQPYFIEANARLQVEHGVTEAVTGIDIVKQQLLIAAGRELQLGQE
DIKLHGWAIEARIYAEDPENGFRAVEGVVTGYREPRGPGVRVDSGAERGLRISTRYDTLLLKVIAWGRDRAEAVARLRAA
LHETVVAGVKTNLELLRTIVESDWFRKAAFHTRILEEKLGELLSQLRARRNIVRAIASRIRAKPLGLTGIAAAKLPVVAS
HGWPWPPWRT

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI189095269, Length=473, Percent_Identity=44.1860465116279, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI65506442, Length=473, Percent_Identity=44.1860465116279, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI295821183, Length=473, Percent_Identity=44.1860465116279, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI106049528, Length=462, Percent_Identity=41.991341991342, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI106049295, Length=462, Percent_Identity=41.991341991342, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI106049292, Length=462, Percent_Identity=41.991341991342, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI116805327, Length=455, Percent_Identity=39.3406593406593, Blast_Score=335, Evalue=8e-92,
Organism=Homo sapiens, GI38679960, Length=509, Percent_Identity=28.6836935166994, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI38679977, Length=509, Percent_Identity=28.6836935166994, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI38679967, Length=509, Percent_Identity=28.6836935166994, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI38679974, Length=509, Percent_Identity=28.6836935166994, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI38679971, Length=509, Percent_Identity=28.6836935166994, Blast_Score=212, Evalue=7e-55,
Organism=Homo sapiens, GI134142062, Length=511, Percent_Identity=29.1585127201566, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI1789654, Length=450, Percent_Identity=41.5555555555556, Blast_Score=330, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17567343, Length=476, Percent_Identity=42.2268907563025, Blast_Score=350, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI17562816, Length=501, Percent_Identity=42.7145708582834, Blast_Score=348, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI71987519, Length=452, Percent_Identity=41.1504424778761, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI71997168, Length=452, Percent_Identity=31.858407079646, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71997163, Length=451, Percent_Identity=32.3725055432373, Blast_Score=198, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI133931226, Length=427, Percent_Identity=29.03981264637, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319685, Length=446, Percent_Identity=40.3587443946188, Blast_Score=332, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6319695, Length=467, Percent_Identity=41.7558886509636, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6321376, Length=467, Percent_Identity=41.7558886509636, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6323863, Length=500, Percent_Identity=29.2, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6324343, Length=512, Percent_Identity=27.5390625, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24652212, Length=448, Percent_Identity=42.8571428571429, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652210, Length=448, Percent_Identity=42.8571428571429, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652214, Length=448, Percent_Identity=42.8571428571429, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI19921944, Length=448, Percent_Identity=42.8571428571429, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652216, Length=448, Percent_Identity=42.8571428571429, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI281363050, Length=448, Percent_Identity=42.8571428571429, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652224, Length=448, Percent_Identity=42.8571428571429, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652222, Length=448, Percent_Identity=42.8571428571429, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652220, Length=448, Percent_Identity=42.8571428571429, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652218, Length=448, Percent_Identity=42.8571428571429, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24651757, Length=451, Percent_Identity=38.3592017738359, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24651759, Length=396, Percent_Identity=39.6464646464646, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI161076407, Length=505, Percent_Identity=29.5049504950495, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24586460, Length=505, Percent_Identity=29.5049504950495, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI161076409, Length=505, Percent_Identity=29.5049504950495, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24586458, Length=505, Percent_Identity=29.5049504950495, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 54457; Mature: 54326

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRVLVATRGEIAIRIARAVRELGWEPITIYAPDDAHSPHVRAGTFSVMVESYTNPDSV
CCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEECCHHEEEEECCCCHHHH
VEAAIKAGADILHPGYGFLSEDPSFARKVLDAGISWAGPKPETMELLGDKHRAKQLAEKV
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHC
GVPTPPWCEARSAEEAVRCAERIGYPVMLKASKAGGGRGMRVASNSEEVRRAYRLVSLEA
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHHHEEEC
RNGFGDAGVVYIEKYVRNPRHIEVQILGDGQGHVVHLYERECSIQRRRQKIIEEAPSPFA
CCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHH
EKVPQVRSRLVDYALRLAEEAEYQSAGTIEFIVDERGQPYFIEANARLQVEHGVTEAVTG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCEEEECCCCHHHHHH
IDIVKQQLLIAAGRELQLGQEDIKLHGWAIEARIYAEDPENGFRAVEGVVTGYREPRGPG
HHHHHHHHHHHCCCCCCCCHHHHEEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCC
VRVDSGAERGLRISTRYDTLLLKVIAWGRDRAEAVARLRAALHETVVAGVKTNLELLRTI
EEECCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VESDWFRKAAFHTRILEEKLGELLSQLRARRNIVRAIASRIRAKPLGLTGIAAAKLPVVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCEEE
SHGWPWPPWRT
CCCCCCCCCCC
>Mature Secondary Structure 
ALRVLVATRGEIAIRIARAVRELGWEPITIYAPDDAHSPHVRAGTFSVMVESYTNPDSV
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEECCHHEEEEECCCCHHHH
VEAAIKAGADILHPGYGFLSEDPSFARKVLDAGISWAGPKPETMELLGDKHRAKQLAEKV
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHC
GVPTPPWCEARSAEEAVRCAERIGYPVMLKASKAGGGRGMRVASNSEEVRRAYRLVSLEA
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHHHEEEC
RNGFGDAGVVYIEKYVRNPRHIEVQILGDGQGHVVHLYERECSIQRRRQKIIEEAPSPFA
CCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHH
EKVPQVRSRLVDYALRLAEEAEYQSAGTIEFIVDERGQPYFIEANARLQVEHGVTEAVTG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCEEEECCCCHHHHHH
IDIVKQQLLIAAGRELQLGQEDIKLHGWAIEARIYAEDPENGFRAVEGVVTGYREPRGPG
HHHHHHHHHHHCCCCCCCCHHHHEEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCC
VRVDSGAERGLRISTRYDTLLLKVIAWGRDRAEAVARLRAALHETVVAGVKTNLELLRTI
EEECCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VESDWFRKAAFHTRILEEKLGELLSQLRARRNIVRAIASRIRAKPLGLTGIAAAKLPVVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCEEE
SHGWPWPPWRT
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]