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The map label for this gene is fdxH [H]

Identifier: 124027920

GI number: 124027920

Start: 1068395

End: 1069189

Strand: Direct

Name: fdxH [H]

Synonym: Hbut_1052

Alternate gene names: 124027920

Gene position: 1068395-1069189 (Clockwise)

Preceding gene: 124027919

Following gene: 124027921

Centisome position: 64.08

GC content: 51.57

Gene sequence:

>795_bases
TTGACCGAGTACGCAATAATGGTGAATATAGACAACTGTATAGGCTGTAGAGCCTGCCAGATAGCATGCAAGGTGTGGAA
TGGGCTTAAAGCAGAACTCACAAGCTTCAACCCTGAAGGCTATACAAACCCTGTGGATCTCACGCCAAACACGTGGATGA
TAATCGAGTTTATCGAAGGTACACGCGGCGGAGAGCCCTACTGGATATTCAAGAAGTTCCAGTGTATGCACTGCAGCGAA
GCACCATGCGCCAAGGCATGCCCTGTAAACGCTATAGAGGTACACCCTGAGGGCGCCGTGGTGATACGTTCCGACAAGTG
TGTTGGCTGCCAATACTGCATAGAGGCGTGCCCGTACGATGTACCACGCTACGACCCGGTGACGAACAAGGTGTACAAGT
GTACACTGTGTATAGACAGGATACAGAATGGACTAGCTCCAGCATGTGTAGAGGCTTGTCCAACGGAGGCACTCGTATTC
GGCCCCTACGACGAGCTTGTAAGGAAGTATCGCGAGGCAGGCTTCGAGATCTATGGCGACAAGGTTAACAGCTATGTTGG
TAGGACACACTACCTCTACGCTACGAGGAAATACAAGGATATCGGTAGTGATGAAGGCAAGAAGTGGCACGAGGTTCTGG
GCCTACCAAGCAATCCACAGGCTGGACTACCGGTCATAAAGGCTGGACGCGATATTGGTGCTGGGCTTGCCGTAGCAGCA
CTCGCCGGCGCAGTACTACACGCTGTATACTGGCGTGCTAAGAGAATTGAGGAGAGAAAGAAGCAGTCATCGTAA

Upstream 100 bases:

>100_bases
TACACGTACAAGTCAGAGCCTAGGATAAAAACAGCTATTGCTTAACCCACCAACAATGTTTAACCCATACACATGGTCCA
TGTAGGGGGTGTGGATAGGC

Downstream 100 bases:

>100_bases
CCCCATGGAGCTGTTTTAGGCTAAAGCTCGCGTGGGGGTGGAACCATGGCTGGTGAGAGAGAGGTAAAGCTGTTTAGCGA
GCTAGAGATATGGGTTCACA

Product: formate dehydrogenase, nitrate-inducible, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit beta; FDH subunit beta [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTEYAIMVNIDNCIGCRACQIACKVWNGLKAELTSFNPEGYTNPVDLTPNTWMIIEFIEGTRGGEPYWIFKKFQCMHCSE
APCAKACPVNAIEVHPEGAVVIRSDKCVGCQYCIEACPYDVPRYDPVTNKVYKCTLCIDRIQNGLAPACVEACPTEALVF
GPYDELVRKYREAGFEIYGDKVNSYVGRTHYLYATRKYKDIGSDEGKKWHEVLGLPSNPQAGLPVIKAGRDIGAGLAVAA
LAGAVLHAVYWRAKRIEERKKQSS

Sequences:

>Translated_264_residues
MTEYAIMVNIDNCIGCRACQIACKVWNGLKAELTSFNPEGYTNPVDLTPNTWMIIEFIEGTRGGEPYWIFKKFQCMHCSE
APCAKACPVNAIEVHPEGAVVIRSDKCVGCQYCIEACPYDVPRYDPVTNKVYKCTLCIDRIQNGLAPACVEACPTEALVF
GPYDELVRKYREAGFEIYGDKVNSYVGRTHYLYATRKYKDIGSDEGKKWHEVLGLPSNPQAGLPVIKAGRDIGAGLAVAA
LAGAVLHAVYWRAKRIEERKKQSS
>Mature_263_residues
TEYAIMVNIDNCIGCRACQIACKVWNGLKAELTSFNPEGYTNPVDLTPNTWMIIEFIEGTRGGEPYWIFKKFQCMHCSEA
PCAKACPVNAIEVHPEGAVVIRSDKCVGCQYCIEACPYDVPRYDPVTNKVYKCTLCIDRIQNGLAPACVEACPTEALVFG
PYDELVRKYREAGFEIYGDKVNSYVGRTHYLYATRKYKDIGSDEGKKWHEVLGLPSNPQAGLPVIKAGRDIGAGLAVAAL
AGAVLHAVYWRAKRIEERKKQSS

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=258, Percent_Identity=36.4341085271318, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1790326, Length=272, Percent_Identity=36.0294117647059, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787122, Length=169, Percent_Identity=33.7278106508876, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787872, Length=169, Percent_Identity=32.5443786982249, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789370, Length=258, Percent_Identity=30.6201550387597, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI2367345, Length=165, Percent_Identity=40.6060606060606, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI226510944, Length=163, Percent_Identity=33.7423312883436, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082179, Length=182, Percent_Identity=32.4175824175824, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI2367245, Length=165, Percent_Identity=31.5151515151515, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788811, Length=160, Percent_Identity=31.875, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082180, Length=147, Percent_Identity=35.3741496598639, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789067, Length=176, Percent_Identity=31.25, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 29333; Mature: 29202

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
6.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYAIMVNIDNCIGCRACQIACKVWNGLKAELTSFNPEGYTNPVDLTPNTWMIIEFIEG
CCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEECC
TRGGEPYWIFKKFQCMHCSEAPCAKACPVNAIEVHPEGAVVIRSDKCVGCQYCIEACPYD
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHCCCC
VPRYDPVTNKVYKCTLCIDRIQNGLAPACVEACPTEALVFGPYDELVRKYREAGFEIYGD
CCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHCCCCEEEECCHHHHHHHHHHCCCEEECC
KVNSYVGRTHYLYATRKYKDIGSDEGKKWHEVLGLPSNPQAGLPVIKAGRDIGAGLAVAA
HHHHHHCCEEEEEEECHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHH
LAGAVLHAVYWRAKRIEERKKQSS
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEYAIMVNIDNCIGCRACQIACKVWNGLKAELTSFNPEGYTNPVDLTPNTWMIIEFIEG
CCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEECC
TRGGEPYWIFKKFQCMHCSEAPCAKACPVNAIEVHPEGAVVIRSDKCVGCQYCIEACPYD
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHCCCC
VPRYDPVTNKVYKCTLCIDRIQNGLAPACVEACPTEALVFGPYDELVRKYREAGFEIYGD
CCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHCCCCEEEECCHHHHHHHHHHCCCEEECC
KVNSYVGRTHYLYATRKYKDIGSDEGKKWHEVLGLPSNPQAGLPVIKAGRDIGAGLAVAA
HHHHHHCCEEEEEEECHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHH
LAGAVLHAVYWRAKRIEERKKQSS
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]