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The map label for this gene is pyk [H]

Identifier: 124027857

GI number: 124027857

Start: 994571

End: 995971

Strand: Direct

Name: pyk [H]

Synonym: Hbut_0985

Alternate gene names: 124027857

Gene position: 994571-995971 (Clockwise)

Preceding gene: 124027856

Following gene: 124027859

Centisome position: 59.66

GC content: 51.89

Gene sequence:

>1401_bases
ATGCAACACTTGAGGGTAAAGATTATAGCCTCTATAGGCCCTTCATCCGGCTCTCCCGAGGTCATACTCAGGCTTGCAGA
GCTTGGTGTTAGCGGCTTCCGCATAAACTTCGCCCATGGTGAGCCGAGTCTTTGGCGTGAATGGGCTGAGTATGTTAGAG
AGGCTGAACGCAAGACTGGGAGGCCGCTAGCCCTTATAGGTGACTTGGTGGGTCCTTCAATCAGGCTTGGAAGGGTGAAG
AATCCGATTAAGCTTAACGCGGGAGACAGGGCTGAGTTTAGATGTGTTGAGGAGAGCGAAGGCGGAGATACGAAGATCAT
ACCGCTGCCCGTAAGGAGGGTCTACGAGGTTCTCGATGAAGGCGACTTAATAGTCATGGATGACGGTAGGGTTAGGCTTA
GGGTTCTCGAGGTATCCGGATATAGTGCTATTGTTGAGGCATTAACACCAGCGACTATAACGTCCAGAAAGGCTATAGCT
ATAAGAGGTAAGGATCCGGGCCTCCCAACACTCTCCCAGCGGGATGTCGAGCATGTAAAGTTCGCCCTTGACAATGGCTT
TGACTACATAGCTCTTAGCCATGTTCGCACTCGTGACGACGTTGACGCGCTTCGTCTCATCGTTCTGCGGGAGGGCGGTG
ACGCGGGTATAGCCGTAAAGATAGAGAATAAGTCGGCAGTAGAGAACCTACAAGACATCATTCGTGCAGCAGATCTCGTA
GTTGTCGCGCGCGGAGACCTAGGGATGACGTATGGTCTTGAGGAGGTTCCAGTACTCCAGGAGCGTATCGTAGCTGCAGC
TAGAAGTGTAGGTAAACCCGTCATAGTCGCTACACAGCTTCTAGAATCGATGATCGAAAACCCTGTCCCAACCAGGGCTG
AGGTTACAGACGTGTATGTTGCGGTGCGGCAAGGTGTGGATGGCCTTATGCTTACCGGCGAGACAGCTATTGGACGCTAC
CCAATTGAAGCTATCCGCTGGTTAAGGAAGATTATTACTCGAGCTGAACAGGTACTACACATTGAACGTTATACCCCCAA
GGATAAGAGATGGGCATACGCTGCAAGCATTGTCGAGACTGCGGAGAAGCTTTCGGCCGCTCTTATTCTCGTCTATAGTA
TAACCGGCAGCCTACCCCCATGGATAGCGGCTTCTCGTCCGATGGTGCCTGTTGTAATCGGTACAGGCAGCCCCCAGAGA
GCACGTAGGCTAGCCGTGCTGTGGGGACTTGATGTCCGCATAGTGGAGGCTGGAGGTTACGAGGAGGGCCTAGCTAAGCT
CGAGGATGTGTTGAGCGAAGAGGGTTTACTATCGGAGGGCGACGTCATTGTTGAGGCTTACCGTGAAACAGAGCATAAGC
AAGTAATTGTCGTTAAAAGGGTCTTTAAAGGGCTTACTTGA

Upstream 100 bases:

>100_bases
GGCGAAGACGCATACCTCATGGCTCGCGAACTCTAGCCCCATCTAGCACCCCATGTTTATAAGTTCTAACCCGACTGTAA
ATCTTCACGGATTGAGTGTC

Downstream 100 bases:

>100_bases
GTCCAAGCTTTTTCAGTGCATAGTATATCGTTGACTCCGGGCGGCCTAGCTGCCTAGCTATCTCATAGACCGTTGCGCCC
TTGAGATATAGCTCGCGTAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MQHLRVKIIASIGPSSGSPEVILRLAELGVSGFRINFAHGEPSLWREWAEYVREAERKTGRPLALIGDLVGPSIRLGRVK
NPIKLNAGDRAEFRCVEESEGGDTKIIPLPVRRVYEVLDEGDLIVMDDGRVRLRVLEVSGYSAIVEALTPATITSRKAIA
IRGKDPGLPTLSQRDVEHVKFALDNGFDYIALSHVRTRDDVDALRLIVLREGGDAGIAVKIENKSAVENLQDIIRAADLV
VVARGDLGMTYGLEEVPVLQERIVAAARSVGKPVIVATQLLESMIENPVPTRAEVTDVYVAVRQGVDGLMLTGETAIGRY
PIEAIRWLRKIITRAEQVLHIERYTPKDKRWAYAASIVETAEKLSAALILVYSITGSLPPWIAASRPMVPVVIGTGSPQR
ARRLAVLWGLDVRIVEAGGYEEGLAKLEDVLSEEGLLSEGDVIVEAYRETEHKQVIVVKRVFKGLT

Sequences:

>Translated_466_residues
MQHLRVKIIASIGPSSGSPEVILRLAELGVSGFRINFAHGEPSLWREWAEYVREAERKTGRPLALIGDLVGPSIRLGRVK
NPIKLNAGDRAEFRCVEESEGGDTKIIPLPVRRVYEVLDEGDLIVMDDGRVRLRVLEVSGYSAIVEALTPATITSRKAIA
IRGKDPGLPTLSQRDVEHVKFALDNGFDYIALSHVRTRDDVDALRLIVLREGGDAGIAVKIENKSAVENLQDIIRAADLV
VVARGDLGMTYGLEEVPVLQERIVAAARSVGKPVIVATQLLESMIENPVPTRAEVTDVYVAVRQGVDGLMLTGETAIGRY
PIEAIRWLRKIITRAEQVLHIERYTPKDKRWAYAASIVETAEKLSAALILVYSITGSLPPWIAASRPMVPVVIGTGSPQR
ARRLAVLWGLDVRIVEAGGYEEGLAKLEDVLSEEGLLSEGDVIVEAYRETEHKQVIVVKRVFKGLT
>Mature_466_residues
MQHLRVKIIASIGPSSGSPEVILRLAELGVSGFRINFAHGEPSLWREWAEYVREAERKTGRPLALIGDLVGPSIRLGRVK
NPIKLNAGDRAEFRCVEESEGGDTKIIPLPVRRVYEVLDEGDLIVMDDGRVRLRVLEVSGYSAIVEALTPATITSRKAIA
IRGKDPGLPTLSQRDVEHVKFALDNGFDYIALSHVRTRDDVDALRLIVLREGGDAGIAVKIENKSAVENLQDIIRAADLV
VVARGDLGMTYGLEEVPVLQERIVAAARSVGKPVIVATQLLESMIENPVPTRAEVTDVYVAVRQGVDGLMLTGETAIGRY
PIEAIRWLRKIITRAEQVLHIERYTPKDKRWAYAASIVETAEKLSAALILVYSITGSLPPWIAASRPMVPVVIGTGSPQR
ARRLAVLWGLDVRIVEAGGYEEGLAKLEDVLSEEGLLSEGDVIVEAYRETEHKQVIVVKRVFKGLT

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=425, Percent_Identity=32.9411764705882, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI33286422, Length=425, Percent_Identity=32.7058823529412, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI33286420, Length=425, Percent_Identity=32.7058823529412, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI32967597, Length=430, Percent_Identity=33.7209302325581, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI10835121, Length=430, Percent_Identity=33.7209302325581, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI310128732, Length=285, Percent_Identity=30.1754385964912, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI310128730, Length=285, Percent_Identity=30.1754385964912, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI310128736, Length=233, Percent_Identity=28.3261802575107, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI310128734, Length=233, Percent_Identity=28.3261802575107, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI310128738, Length=212, Percent_Identity=27.3584905660377, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787965, Length=449, Percent_Identity=31.4031180400891, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1788160, Length=342, Percent_Identity=36.2573099415205, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17544584, Length=419, Percent_Identity=31.7422434367542, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17506829, Length=421, Percent_Identity=31.5914489311164, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI71984413, Length=421, Percent_Identity=31.5914489311164, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI71984406, Length=421, Percent_Identity=31.5914489311164, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17506831, Length=421, Percent_Identity=31.5914489311164, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319279, Length=418, Percent_Identity=35.4066985645933, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=418, Percent_Identity=34.4497607655502, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24648964, Length=469, Percent_Identity=31.5565031982942, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28571814, Length=469, Percent_Identity=31.5565031982942, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24648966, Length=399, Percent_Identity=32.3308270676692, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24581235, Length=469, Percent_Identity=28.3582089552239, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24646914, Length=295, Percent_Identity=32.8813559322034, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51167; Mature: 51167

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLRVKIIASIGPSSGSPEVILRLAELGVSGFRINFAHGEPSLWREWAEYVREAERKTG
CCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
RPLALIGDLVGPSIRLGRVKNPIKLNAGDRAEFRCVEESEGGDTKIIPLPVRRVYEVLDE
CCEEEEHHHCCCCEEECCCCCCEEECCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHCC
GDLIVMDDGRVRLRVLEVSGYSAIVEALTPATITSRKAIAIRGKDPGLPTLSQRDVEHVK
CCEEEEECCCEEEEEEEECCHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCCCHHHHH
FALDNGFDYIALSHVRTRDDVDALRLIVLREGGDAGIAVKIENKSAVENLQDIIRAADLV
HHHCCCCCEEEEECCCCCCCHHHEEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCEE
VVARGDLGMTYGLEEVPVLQERIVAAARSVGKPVIVATQLLESMIENPVPTRAEVTDVYV
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
AVRQGVDGLMLTGETAIGRYPIEAIRWLRKIITRAEQVLHIERYTPKDKRWAYAASIVET
HHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCCCHHHHHHHHHHH
AEKLSAALILVYSITGSLPPWIAASRPMVPVVIGTGSPQRARRLAVLWGLDVRIVEAGGY
HHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHEEEEEECCEEEEEECCCC
EEGLAKLEDVLSEEGLLSEGDVIVEAYRETEHKQVIVVKRVFKGLT
HHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCCEEEEHHHHHCCCC
>Mature Secondary Structure
MQHLRVKIIASIGPSSGSPEVILRLAELGVSGFRINFAHGEPSLWREWAEYVREAERKTG
CCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
RPLALIGDLVGPSIRLGRVKNPIKLNAGDRAEFRCVEESEGGDTKIIPLPVRRVYEVLDE
CCEEEEHHHCCCCEEECCCCCCEEECCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHCC
GDLIVMDDGRVRLRVLEVSGYSAIVEALTPATITSRKAIAIRGKDPGLPTLSQRDVEHVK
CCEEEEECCCEEEEEEEECCHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCCCHHHHH
FALDNGFDYIALSHVRTRDDVDALRLIVLREGGDAGIAVKIENKSAVENLQDIIRAADLV
HHHCCCCCEEEEECCCCCCCHHHEEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCEE
VVARGDLGMTYGLEEVPVLQERIVAAARSVGKPVIVATQLLESMIENPVPTRAEVTDVYV
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
AVRQGVDGLMLTGETAIGRYPIEAIRWLRKIITRAEQVLHIERYTPKDKRWAYAASIVET
HHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCCCHHHHHHHHHHH
AEKLSAALILVYSITGSLPPWIAASRPMVPVVIGTGSPQRARRLAVLWGLDVRIVEAGGY
HHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHEEEEEECCEEEEEECCCC
EEGLAKLEDVLSEEGLLSEGDVIVEAYRETEHKQVIVVKRVFKGLT
HHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCCEEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]