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The map label for this gene is polB [H]

Identifier: 124027770

GI number: 124027770

Start: 898203

End: 900617

Strand: Direct

Name: polB [H]

Synonym: Hbut_0896

Alternate gene names: 124027770

Gene position: 898203-900617 (Clockwise)

Preceding gene: 124027769

Following gene: 124027771

Centisome position: 53.88

GC content: 48.99

Gene sequence:

>2415_bases
TTGGCCGAGGAGATAGAGTTTGTACTCCTAGATTCTAGCTACGAAATAGTCGGAAAGGAACCAGTAATCGTTCTTTGGGG
TGTGACGCTTAGCGGTGAGCGTGTGGTTCTCCTGGATCGCAGGTTCCGACCCTACTTCTACGCGCTCATAAAGCGTGGTT
TTGAAGAAAATGCAACAGCAATAGCAGCTGCTATAAGGAGGCTTAGCAAGGGTTCAAGCCCAATAATAGCTTTAGAGGTT
GTAGACAAGAAGTATTTTGGTAGGCCGCGCAAGGCTGTCCGAGTAACGACTGTGATACCCGAGAGTGTCAGAGAGTACCG
AGAGGCAGTCAAGAAGATAGATGGTGTAGAGGATGCCCTTGAGGCAGATATAAGGTTTGCAATGAGATACATTATAGACA
AGAGGCTATACCCTTTCATGGTGTACCGTGTACCAGTAGAGGATGTTGGTAAGAATCCAGGTTACCGTGTAGACAAAGTA
TTCTACGTTACCGGTGAGCCCCAGAGGATAACAGATATAACGAGAATAGACATGCCCGATCTAAGGCTAACAGCATTCGA
CATAGAGGTCTACAACAAGAGTGGGAGCCCAAATCCTAATCGAGATCCTGTCATAATAATAGCTGTTAAGAGCAGTAATG
GAGACGAGAAGCTCTTTGAAGCAGACGGGTATGATGATAGGAAGGCTATCAGAGGCTTTGTGGATTACATAAAGTCCTTT
GACCCAGACATAATAGTAGGCTATAACAGCAACGGGTTCGACTGGCCATACCTCATGCAGCGAGCACGCAAGGTAGGAGT
TAGGCTGGATGTAACACGTCGTGTTGGTGCAGAGCCGACTACAAGCGTCTATGGTCACGTGTCGGTGCCAGGCAGGCTTA
ACGTAGACCTCTACCACTACGCTGAGGAAATCCATGAGGTTAAGGTTAAGACTCTCGAGGAGGTTGCAGAATATCTTGGA
GTAATGAAGAAGAGTGAACGTGTCCTCATACACTGGTGGGAGATACCGAGGTACTGGGAGGACCCGGAAAAGAGGCCAAT
ACTAGAGCAATATGCGCGAGACGATGTAAGAGCCACCTACGGCTTGGCCGAGAAGATGCTTCCATTCGCGATACAGCTCT
CAACAGTAACCGGTATACCCCTAGACCAGGTTGGAGTAATGGGTGTCGGTTTCCGGCTCGAATGGTATCTCATACGCGCA
GCTTATGAAATGAATGAGCTTGTACCAAACCGTGTCGAAAGGCACGAGGAGAGCTACCGCGGCGCGGTAGTCCTTAAGCC
CCTTAAGGGTGTACACGAAAATGTTGTCGTACTAGACTTTAGTTCGATGTATCCCAACATAATGATAAAATACAATGTTG
GCCCTGACACTATAGTAGACGACCCGGCCGAGTGCGAAAAGTATGGAGGTTGCTACGTTGCACCGGAGGTTGGCCACCGG
TTTAGGAAGCAGCCGCCAGGCTTCTTCAAGACGGTCCTGGAAAACCTGCTAAGGCTTAGGAAGCAGGTTAGAGAGAAAAT
GAAGGAATTACCGCCAGAGAGTCCAGAGTATAGACTTTATGATGAGAGGCAGAGGGCCCTCAAGGTATTGGCTAACGCTA
GCTACGGCTACATGGGCTGGAGTGGAGCAAGGTGGTATTGTAAACGGTGCGCTGAGGCGGTAACAGCGTGGGGTCGGAGC
CTCATACTCTCAGCCATAGAGTATGCTCGTAAGCTAGGCCTCAAGGTCATATATGGTGATACAGACTCCCTATTCGTAAC
ATATGACCCCGAGAAGGTTAAGAAGCTGATAGACTATGTGGTGAATGTGTTGGGCTTTGAGATAAAGATAGACAAGATAT
ACCGTCGAGTATTCTTCACAGAGGCGAAGAAGAGGTATGTAGGTCTACTGGAGGACGGAAGGATAGACATAGTAGGCTTT
GAGGCTGTGCGTGGTGATTGGTGTGAGTTAGCCAAGGAGGTTCAGTCAACAGTAGCAGAGATTGTGCTGAAAACAGGCAA
GATTGACGAAGCTATACGCTATATACGCGACGTGATAAAGAAGCTTAGGGAGGGCGAGATACCGGTCGAGAAGCTGATAA
TATGGAAGACGTTATCCAAGAGGCTGGAAGAGTACGAACACGAGGCGCCTCATGTAGTTGCTGCGAGGAGGATGAAGGAG
GCTGGGTACGATGTCTCACCTGGCGACAAGATAGGCTACGTAATAGTACGTGGCGCGGGCAGAATATCGAGCAGAGCATA
CCCCTACTTCATGGTGAAGCCTTCGGACATAGATGTAGATTACTACATAGACCACCAGATAGTACCTGCCGTATTGAGAA
TCCTCAGCTACTTTGGCGTAACAGAGAAGCAGCTCAAAGCAGCATCAACGGGGCACCGGACGCTGTTCGACTTCATGCGG
GGTAAGCGTGGCTAG

Upstream 100 bases:

>100_bases
GAGGCATGCCCTTGTAATGCTGCGCTATGAGGCGTATAGCTTGGCTCTCTAAGGGTTATTAGCGAAGTAGGTGTACAGTA
TTACCGGGGACGCTATAGCC

Downstream 100 bases:

>100_bases
CCGTTAGGTAGAATAGGCCGGAAACGCTGGTAGCTCCATACGTATAGGCTGGGAATGAAGCAGTACAGCAGGGTGTAGGC
GTTGATACTAGCATCGTGGC

Product: DNA polymerase II

Products: NA

Alternate protein names: DNA polymerase II [H]

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MAEEIEFVLLDSSYEIVGKEPVIVLWGVTLSGERVVLLDRRFRPYFYALIKRGFEENATAIAAAIRRLSKGSSPIIALEV
VDKKYFGRPRKAVRVTTVIPESVREYREAVKKIDGVEDALEADIRFAMRYIIDKRLYPFMVYRVPVEDVGKNPGYRVDKV
FYVTGEPQRITDITRIDMPDLRLTAFDIEVYNKSGSPNPNRDPVIIIAVKSSNGDEKLFEADGYDDRKAIRGFVDYIKSF
DPDIIVGYNSNGFDWPYLMQRARKVGVRLDVTRRVGAEPTTSVYGHVSVPGRLNVDLYHYAEEIHEVKVKTLEEVAEYLG
VMKKSERVLIHWWEIPRYWEDPEKRPILEQYARDDVRATYGLAEKMLPFAIQLSTVTGIPLDQVGVMGVGFRLEWYLIRA
AYEMNELVPNRVERHEESYRGAVVLKPLKGVHENVVVLDFSSMYPNIMIKYNVGPDTIVDDPAECEKYGGCYVAPEVGHR
FRKQPPGFFKTVLENLLRLRKQVREKMKELPPESPEYRLYDERQRALKVLANASYGYMGWSGARWYCKRCAEAVTAWGRS
LILSAIEYARKLGLKVIYGDTDSLFVTYDPEKVKKLIDYVVNVLGFEIKIDKIYRRVFFTEAKKRYVGLLEDGRIDIVGF
EAVRGDWCELAKEVQSTVAEIVLKTGKIDEAIRYIRDVIKKLREGEIPVEKLIIWKTLSKRLEEYEHEAPHVVAARRMKE
AGYDVSPGDKIGYVIVRGAGRISSRAYPYFMVKPSDIDVDYYIDHQIVPAVLRILSYFGVTEKQLKAASTGHRTLFDFMR
GKRG

Sequences:

>Translated_804_residues
MAEEIEFVLLDSSYEIVGKEPVIVLWGVTLSGERVVLLDRRFRPYFYALIKRGFEENATAIAAAIRRLSKGSSPIIALEV
VDKKYFGRPRKAVRVTTVIPESVREYREAVKKIDGVEDALEADIRFAMRYIIDKRLYPFMVYRVPVEDVGKNPGYRVDKV
FYVTGEPQRITDITRIDMPDLRLTAFDIEVYNKSGSPNPNRDPVIIIAVKSSNGDEKLFEADGYDDRKAIRGFVDYIKSF
DPDIIVGYNSNGFDWPYLMQRARKVGVRLDVTRRVGAEPTTSVYGHVSVPGRLNVDLYHYAEEIHEVKVKTLEEVAEYLG
VMKKSERVLIHWWEIPRYWEDPEKRPILEQYARDDVRATYGLAEKMLPFAIQLSTVTGIPLDQVGVMGVGFRLEWYLIRA
AYEMNELVPNRVERHEESYRGAVVLKPLKGVHENVVVLDFSSMYPNIMIKYNVGPDTIVDDPAECEKYGGCYVAPEVGHR
FRKQPPGFFKTVLENLLRLRKQVREKMKELPPESPEYRLYDERQRALKVLANASYGYMGWSGARWYCKRCAEAVTAWGRS
LILSAIEYARKLGLKVIYGDTDSLFVTYDPEKVKKLIDYVVNVLGFEIKIDKIYRRVFFTEAKKRYVGLLEDGRIDIVGF
EAVRGDWCELAKEVQSTVAEIVLKTGKIDEAIRYIRDVIKKLREGEIPVEKLIIWKTLSKRLEEYEHEAPHVVAARRMKE
AGYDVSPGDKIGYVIVRGAGRISSRAYPYFMVKPSDIDVDYYIDHQIVPAVLRILSYFGVTEKQLKAASTGHRTLFDFMR
GKRG
>Mature_803_residues
AEEIEFVLLDSSYEIVGKEPVIVLWGVTLSGERVVLLDRRFRPYFYALIKRGFEENATAIAAAIRRLSKGSSPIIALEVV
DKKYFGRPRKAVRVTTVIPESVREYREAVKKIDGVEDALEADIRFAMRYIIDKRLYPFMVYRVPVEDVGKNPGYRVDKVF
YVTGEPQRITDITRIDMPDLRLTAFDIEVYNKSGSPNPNRDPVIIIAVKSSNGDEKLFEADGYDDRKAIRGFVDYIKSFD
PDIIVGYNSNGFDWPYLMQRARKVGVRLDVTRRVGAEPTTSVYGHVSVPGRLNVDLYHYAEEIHEVKVKTLEEVAEYLGV
MKKSERVLIHWWEIPRYWEDPEKRPILEQYARDDVRATYGLAEKMLPFAIQLSTVTGIPLDQVGVMGVGFRLEWYLIRAA
YEMNELVPNRVERHEESYRGAVVLKPLKGVHENVVVLDFSSMYPNIMIKYNVGPDTIVDDPAECEKYGGCYVAPEVGHRF
RKQPPGFFKTVLENLLRLRKQVREKMKELPPESPEYRLYDERQRALKVLANASYGYMGWSGARWYCKRCAEAVTAWGRSL
ILSAIEYARKLGLKVIYGDTDSLFVTYDPEKVKKLIDYVVNVLGFEIKIDKIYRRVFFTEAKKRYVGLLEDGRIDIVGFE
AVRGDWCELAKEVQSTVAEIVLKTGKIDEAIRYIRDVIKKLREGEIPVEKLIIWKTLSKRLEEYEHEAPHVVAARRMKEA
GYDVSPGDKIGYVIVRGAGRISSRAYPYFMVKPSDIDVDYYIDHQIVPAVLRILSYFGVTEKQLKAASTGHRTLFDFMRG
KRG

Specific function: Thought To Be Involved In DNA Repair And/Or Mutagenesis. [C]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=867, Percent_Identity=26.9896193771626, Blast_Score=228, Evalue=2e-59,
Organism=Homo sapiens, GI106507301, Length=619, Percent_Identity=28.7560581583199, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI153792012, Length=632, Percent_Identity=25.1582278481013, Blast_Score=151, Evalue=3e-36,
Organism=Escherichia coli, GI1786246, Length=648, Percent_Identity=25.1543209876543, Blast_Score=134, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17559488, Length=865, Percent_Identity=24.8554913294798, Blast_Score=197, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI32565317, Length=607, Percent_Identity=25.3706754530478, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI86563326, Length=622, Percent_Identity=24.7588424437299, Blast_Score=122, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320101, Length=669, Percent_Identity=29.1479820627803, Blast_Score=232, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324227, Length=631, Percent_Identity=24.8811410459588, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325090, Length=624, Percent_Identity=23.5576923076923, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24664937, Length=879, Percent_Identity=25.8248009101251, Blast_Score=201, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24648780, Length=631, Percent_Identity=25.6735340729002, Blast_Score=175, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24586371, Length=637, Percent_Identity=23.3908948194662, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR004578
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 92519; Mature: 92388

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEIEFVLLDSSYEIVGKEPVIVLWGVTLSGERVVLLDRRFRPYFYALIKRGFEENATA
CCCCEEEEEECCCCEEECCCCEEEEEEEEECCCEEEEEECCCCHHHHHHHHCCCCCCHHH
IAAAIRRLSKGSSPIIALEVVDKKYFGRPRKAVRVTTVIPESVREYREAVKKIDGVEDAL
HHHHHHHHHCCCCCEEEEEEECHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHCCHHHHH
EADIRFAMRYIIDKRLYPFMVYRVPVEDVGKNPGYRVDKVFYVTGEPQRITDITRIDMPD
HHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCC
LRLTAFDIEVYNKSGSPNPNRDPVIIIAVKSSNGDEKLFEADGYDDRKAIRGFVDYIKSF
CEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCC
DPDIIVGYNSNGFDWPYLMQRARKVGVRLDVTRRVGAEPTTSVYGHVSVPGRLNVDLYHY
CCCEEEEECCCCCCHHHHHHHHHHCCCEEEEHHHCCCCCCCCEEEEEECCCEECCHHHHH
AEEIHEVKVKTLEEVAEYLGVMKKSERVLIHWWEIPRYWEDPEKRPILEQYARDDVRATY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
GLAEKMLPFAIQLSTVTGIPLDQVGVMGVGFRLEWYLIRAAYEMNELVPNRVERHEESYR
HHHHHHCCEEEEEEEECCCCHHHCCCEEECEEEEEEEEEHHHHHHHHCHHHHHHHHHHHC
GAVVLKPLKGVHENVVVLDFSSMYPNIMIKYNVGPDTIVDDPAECEKYGGCYVAPEVGHR
CEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEHHHHHH
FRKQPPGFFKTVLENLLRLRKQVREKMKELPPESPEYRLYDERQRALKVLANASYGYMGW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
SGARWYCKRCAEAVTAWGRSLILSAIEYARKLGLKVIYGDTDSLFVTYDPEKVKKLIDYV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECHHHHHHHHHHH
VNVLGFEIKIDKIYRRVFFTEAKKRYVGLLEDGRIDIVGFEAVRGDWCELAKEVQSTVAE
HHHHCCEEEHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEHHHCCHHHHHHHHHHHHHHH
IVLKTGKIDEAIRYIRDVIKKLREGEIPVEKLIIWKTLSKRLEEYEHEAPHVVAARRMKE
HHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGYDVSPGDKIGYVIVRGAGRISSRAYPYFMVKPSDIDVDYYIDHQIVPAVLRILSYFGV
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHCC
TEKQLKAASTGHRTLFDFMRGKRG
CHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
AEEIEFVLLDSSYEIVGKEPVIVLWGVTLSGERVVLLDRRFRPYFYALIKRGFEENATA
CCCEEEEEECCCCEEECCCCEEEEEEEEECCCEEEEEECCCCHHHHHHHHCCCCCCHHH
IAAAIRRLSKGSSPIIALEVVDKKYFGRPRKAVRVTTVIPESVREYREAVKKIDGVEDAL
HHHHHHHHHCCCCCEEEEEEECHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHCCHHHHH
EADIRFAMRYIIDKRLYPFMVYRVPVEDVGKNPGYRVDKVFYVTGEPQRITDITRIDMPD
HHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCC
LRLTAFDIEVYNKSGSPNPNRDPVIIIAVKSSNGDEKLFEADGYDDRKAIRGFVDYIKSF
CEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCC
DPDIIVGYNSNGFDWPYLMQRARKVGVRLDVTRRVGAEPTTSVYGHVSVPGRLNVDLYHY
CCCEEEEECCCCCCHHHHHHHHHHCCCEEEEHHHCCCCCCCCEEEEEECCCEECCHHHHH
AEEIHEVKVKTLEEVAEYLGVMKKSERVLIHWWEIPRYWEDPEKRPILEQYARDDVRATY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
GLAEKMLPFAIQLSTVTGIPLDQVGVMGVGFRLEWYLIRAAYEMNELVPNRVERHEESYR
HHHHHHCCEEEEEEEECCCCHHHCCCEEECEEEEEEEEEHHHHHHHHCHHHHHHHHHHHC
GAVVLKPLKGVHENVVVLDFSSMYPNIMIKYNVGPDTIVDDPAECEKYGGCYVAPEVGHR
CEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEHHHHHH
FRKQPPGFFKTVLENLLRLRKQVREKMKELPPESPEYRLYDERQRALKVLANASYGYMGW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
SGARWYCKRCAEAVTAWGRSLILSAIEYARKLGLKVIYGDTDSLFVTYDPEKVKKLIDYV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECHHHHHHHHHHH
VNVLGFEIKIDKIYRRVFFTEAKKRYVGLLEDGRIDIVGFEAVRGDWCELAKEVQSTVAE
HHHHCCEEEHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEHHHCCHHHHHHHHHHHHHHH
IVLKTGKIDEAIRYIRDVIKKLREGEIPVEKLIIWKTLSKRLEEYEHEAPHVVAARRMKE
HHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGYDVSPGDKIGYVIVRGAGRISSRAYPYFMVKPSDIDVDYYIDHQIVPAVLRILSYFGV
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHCC
TEKQLKAASTGHRTLFDFMRGKRG
CHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]