Click here to switch to the map view.

The map label for this gene is gcvT [H]

Identifier: 124027706

GI number: 124027706

Start: 825920

End: 827056

Strand: Direct

Name: gcvT [H]

Synonym: Hbut_0829

Alternate gene names: 124027706

Gene position: 825920-827056 (Clockwise)

Preceding gene: 124027703

Following gene: 124027707

Centisome position: 49.54

GC content: 55.32

Gene sequence:

>1137_bases
GTGACGGGTGTTAAGGTTCCCCTATACGATGTTCATAGGGAGCTTGGTGCATCGCTTGGAGAGTTTGCTGGGTGGCTAGT
CCCCATAGATTATGGCAGTATTGTTGAGGAGCATGTTGCTGTGAGGAAGACTGTTGGGTTCTTCGATCTAAGCCATATGG
CTAGGATAATCGTGTCAGGCCCCGATGCCGGCAAGCTGTTGGACAAGCTGGTGCCACGCTATCTTGAGTCAGAGCCAGGC
ACTATGCTTGGTCCAACAGCGTTCCTAAATGAGAACGCTGGCTTTGTTGACGATGTGATGCTCTACAACCTTGGCGGTAA
CCAGTGGATGATTGTTGCAAACGCTGTTAACCGGGAGAAAGTGCTAGGCTGGCTCAACGATTGGCTCTCAAGGCTAGGGT
TTACAGCCAGCGTTGAGGACAAGACGTTGGAGCTAGCCATGTTTGCCGTCCAGGGTCCAAAGGCGGCTGAGCTTATGGAG
AGGCTTGGTGCACCACGCGAGGTCTTGGAGCTGAAGCTGTTGAGGTTCCGGCTCAACGTAGAGCTATCAGAGGCAAAGGC
AAGGGCATTTCTTGTTTCGCGTAGCGGGTGGACAGGCGAGGATGGCTTCGAGATCATAGCCCCAGTTGGTGAGGCTGAGA
AGATACTCCGCAAGGCTGCTGAGATAGTTCGGGAGCTCGGAGGCAGACTATGCGGACTAGGCGCGAGGGATAGCTTGAGA
ATGGAGATGGGCTTCGTACTCTACGGCCACGAGATAGACGAGGAAACAACACCCGTCGATGCAAGGTACTGGTGGGTCTA
CCAGCCCGGCCCAAAGGAGGACTGCGTGGGCTGCAAGGCTCTTCGCGAGGCTCTACGGCGCGGCGCTGTAAAGGTAAGAG
TGGGGATTAGGCTCTCGAAGAAGGCCCGCATAGTGCCACGGCAGGGAGACAAGATATACGTTGAGGGTGTTGAGGTAGGC
CATGTGACCAGCGGCGCCTATAGTCCAGTCCTCGGCCGGAGCATAGCACAAGCATACATAAAGCCAAGCCACGCCCTCAT
GGGGCTCACCGTCGAGGTTGAACGGAGAGGCAAGAGGTACCAGGGCAAGATAGTCGACTTCCCACTAGTAAAGCCTACAT
CCTCACCACCACTCTAG

Upstream 100 bases:

>100_bases
AGCTTGACAGGAGAGTGGCACAGAAGACAACGTTCAAACGGGCCCAGCGTGCCTAGTTTCAGCGTAGATGCTGGCTAGCG
CTGTTTTGGGGTGGGCTAGT

Downstream 100 bases:

>100_bases
CCATGGTGCTCCTTAAACGACAAAGAAAATCGGGACTCACAAGGCCACCGAGCAGTTTTACCCACTCACATCCATCAACC
TAGACCCTATCCTCATAATA

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTGVKVPLYDVHRELGASLGEFAGWLVPIDYGSIVEEHVAVRKTVGFFDLSHMARIIVSGPDAGKLLDKLVPRYLESEPG
TMLGPTAFLNENAGFVDDVMLYNLGGNQWMIVANAVNREKVLGWLNDWLSRLGFTASVEDKTLELAMFAVQGPKAAELME
RLGAPREVLELKLLRFRLNVELSEAKARAFLVSRSGWTGEDGFEIIAPVGEAEKILRKAAEIVRELGGRLCGLGARDSLR
MEMGFVLYGHEIDEETTPVDARYWWVYQPGPKEDCVGCKALREALRRGAVKVRVGIRLSKKARIVPRQGDKIYVEGVEVG
HVTSGAYSPVLGRSIAQAYIKPSHALMGLTVEVERRGKRYQGKIVDFPLVKPTSSPPL

Sequences:

>Translated_378_residues
MTGVKVPLYDVHRELGASLGEFAGWLVPIDYGSIVEEHVAVRKTVGFFDLSHMARIIVSGPDAGKLLDKLVPRYLESEPG
TMLGPTAFLNENAGFVDDVMLYNLGGNQWMIVANAVNREKVLGWLNDWLSRLGFTASVEDKTLELAMFAVQGPKAAELME
RLGAPREVLELKLLRFRLNVELSEAKARAFLVSRSGWTGEDGFEIIAPVGEAEKILRKAAEIVRELGGRLCGLGARDSLR
MEMGFVLYGHEIDEETTPVDARYWWVYQPGPKEDCVGCKALREALRRGAVKVRVGIRLSKKARIVPRQGDKIYVEGVEVG
HVTSGAYSPVLGRSIAQAYIKPSHALMGLTVEVERRGKRYQGKIVDFPLVKPTSSPPL
>Mature_377_residues
TGVKVPLYDVHRELGASLGEFAGWLVPIDYGSIVEEHVAVRKTVGFFDLSHMARIIVSGPDAGKLLDKLVPRYLESEPGT
MLGPTAFLNENAGFVDDVMLYNLGGNQWMIVANAVNREKVLGWLNDWLSRLGFTASVEDKTLELAMFAVQGPKAAELMER
LGAPREVLELKLLRFRLNVELSEAKARAFLVSRSGWTGEDGFEIIAPVGEAEKILRKAAEIVRELGGRLCGLGARDSLRM
EMGFVLYGHEIDEETTPVDARYWWVYQPGPKEDCVGCKALREALRRGAVKVRVGIRLSKKARIVPRQGDKIYVEGVEVGH
VTSGAYSPVLGRSIAQAYIKPSHALMGLTVEVERRGKRYQGKIVDFPLVKPTSSPPL

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=377, Percent_Identity=32.6259946949602, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI257796258, Length=357, Percent_Identity=32.7731092436975, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI257796254, Length=373, Percent_Identity=30.2949061662198, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI257796256, Length=327, Percent_Identity=31.8042813455657, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI24797151, Length=352, Percent_Identity=24.7159090909091, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI194306651, Length=359, Percent_Identity=24.2339832869081, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789272, Length=351, Percent_Identity=33.6182336182336, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17560118, Length=383, Percent_Identity=32.1148825065274, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI32563613, Length=395, Percent_Identity=25.3164556962025, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320222, Length=391, Percent_Identity=35.0383631713555, Blast_Score=173, Evalue=4e-44,
Organism=Drosophila melanogaster, GI20129441, Length=378, Percent_Identity=32.2751322751323, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI20130091, Length=355, Percent_Identity=24.2253521126761, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571104, Length=366, Percent_Identity=21.8579234972678, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 41758; Mature: 41627

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVKVPLYDVHRELGASLGEFAGWLVPIDYGSIVEEHVAVRKTVGFFDLSHMARIIVSG
CCCCCCHHHHHHHHHCCCHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
PDAGKLLDKLVPRYLESEPGTMLGPTAFLNENAGFVDDVMLYNLGGNQWMIVANAVNREK
CCHHHHHHHHHHHHHCCCCCCEECCHHEECCCCCCCEEEEEEECCCCEEEEEEECCCHHH
VLGWLNDWLSRLGFTASVEDKTLELAMFAVQGPKAAELMERLGAPREVLELKLLRFRLNV
HHHHHHHHHHHCCCEECCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCE
ELSEAKARAFLVSRSGWTGEDGFEIIAPVGEAEKILRKAAEIVRELGGRLCGLGARDSLR
EEHHCCEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCCCCEE
MEMGFVLYGHEIDEETTPVDARYWWVYQPGPKEDCVGCKALREALRRGAVKVRVGIRLSK
EEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECC
KARIVPRQGDKIYVEGVEVGHVTSGAYSPVLGRSIAQAYIKPSHALMGLTVEVERRGKRY
CEEECCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCC
QGKIVDFPLVKPTSSPPL
CCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
TGVKVPLYDVHRELGASLGEFAGWLVPIDYGSIVEEHVAVRKTVGFFDLSHMARIIVSG
CCCCCHHHHHHHHHCCCHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
PDAGKLLDKLVPRYLESEPGTMLGPTAFLNENAGFVDDVMLYNLGGNQWMIVANAVNREK
CCHHHHHHHHHHHHHCCCCCCEECCHHEECCCCCCCEEEEEEECCCCEEEEEEECCCHHH
VLGWLNDWLSRLGFTASVEDKTLELAMFAVQGPKAAELMERLGAPREVLELKLLRFRLNV
HHHHHHHHHHHCCCEECCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCE
ELSEAKARAFLVSRSGWTGEDGFEIIAPVGEAEKILRKAAEIVRELGGRLCGLGARDSLR
EEHHCCEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCCCCEE
MEMGFVLYGHEIDEETTPVDARYWWVYQPGPKEDCVGCKALREALRRGAVKVRVGIRLSK
EEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECC
KARIVPRQGDKIYVEGVEVGHVTSGAYSPVLGRSIAQAYIKPSHALMGLTVEVERRGKRY
CEEECCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCC
QGKIVDFPLVKPTSSPPL
CCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]