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The map label for this gene is ycjG [C]

Identifier: 124027565

GI number: 124027565

Start: 665947

End: 667128

Strand: Reverse

Name: ycjG [C]

Synonym: Hbut_0684

Alternate gene names: 124027565

Gene position: 667128-665947 (Counterclockwise)

Preceding gene: 124027570

Following gene: 124027564

Centisome position: 40.02

GC content: 59.48

Gene sequence:

>1182_bases
TTGCCGCGTATCAGCCGAGTTGAATACTTTGTCGTCGAGGCTAGGATTCGTGGAGTCTTCCGCATAGCCTACTCTGCGGC
TAGCGTATCTAGGAGCGTGGTTGTCAAGGTTGTGCTTGAGGATGGCACTATTGGGTTTGGCGAGGCTTCTCCAAGCAAGA
TGGTTACATGGGAGACCATAGACTCTATTGGGTCGTACGTGGGGCTTGTCTCGGGGCAGCTGAGGGGCCTCTCTCTGCCA
GAGGAGATGGGCAAGGCTCTACGCGTGATCCACGGGTATGGGCTTGGGTTTAGCTCTGCTAGGGCTGCGTTGGAGTCGGC
AATACTTGATGCTGTAGGTAAGCTGTTTGGAGTGCCTGTATACAATCTGCTTGGTGGCCGTGTTGCTGAGAGGCTGGTTA
CCGACTACACGGTATCAATACCGGATCTCGGGGTGGCTAGGGATATTGCTGGGAGGAGTGGCCGTGGCTGGGAGCTATAC
GCCGAGACTATAGAGTACATTGTTGGGCTACGCTCTAAGCCCCCGGAGGAGCCGCCCATACCTGTACCGTCGATCCAGGG
GTTCAACGTGCTTAAGGTGAAGGTTGGGACAGGTGATCCCTTGCTTGACGCGCAGCTTGTGCTAGCTGCTTATGAGGCCT
CGAGGGGCCGTGCCAGGATACGTGTGGACGCTAACCAGGCCTGGTCCCCAAAGGAGGCTGTCCGTGTAATACATAGGCTC
GAGAACACACTCGGCGACGCCCTAGAGCTTGTTGAGCAGCCCGTGCCAGCATGGAACCTCGAGGGACTACGCTACGTGAA
GGAGCGTGTGGACGTGCCGATTGCAGCGGACGAGTCCGCGCGGAGCCCCGTTGAGGCCGCCAGGGTGGCCGCCATGGGCG
CAGCCGACGTCATCAACATTAAGATAGCAAAGGCTGGCGGGCCACTCCAGGCCGCCAGAATCGCATCGCTAGCTGAGGCT
CACGGGCTCCAGGTCATGTGGGGCTGCATGGCGGAGACAGGGCTCGGCATAGCCCAGGCGCTACATGCAGCCCTTACCAG
CACTGCCACCCGCTACGTGGACCTCGACTCGCCGCTCTTCCTCGAGAAAGACCCTGCCAGGAACTCGCCGAGATACAGGG
CTACAGCGGAGGGTGTAACTGTGGAGCCAGCTCCAGGCCCCGGCCTTGCTACAGAGATCTAG

Upstream 100 bases:

>100_bases
GGAGAGACTATGGAGGCTTCTCCATGGAGGGGCCTCTAATAGTCACTATTATAATTATTCTCGGTCTCGGCCACTAGTTA
CGCTGTGGAGGCCTGCAGTC

Downstream 100 bases:

>100_bases
GACCCCAACACACTTTTCTACATGATCGCCCTCTCAACCATGCCCATAGTAGATAACCTCTACAGGCTCGAGAGTTATGA
GGCCCTCACCTACAACCTTC

Product: muconate cycloisomerase I

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MPRISRVEYFVVEARIRGVFRIAYSAASVSRSVVVKVVLEDGTIGFGEASPSKMVTWETIDSIGSYVGLVSGQLRGLSLP
EEMGKALRVIHGYGLGFSSARAALESAILDAVGKLFGVPVYNLLGGRVAERLVTDYTVSIPDLGVARDIAGRSGRGWELY
AETIEYIVGLRSKPPEEPPIPVPSIQGFNVLKVKVGTGDPLLDAQLVLAAYEASRGRARIRVDANQAWSPKEAVRVIHRL
ENTLGDALELVEQPVPAWNLEGLRYVKERVDVPIAADESARSPVEAARVAAMGAADVINIKIAKAGGPLQAARIASLAEA
HGLQVMWGCMAETGLGIAQALHAALTSTATRYVDLDSPLFLEKDPARNSPRYRATAEGVTVEPAPGPGLATEI

Sequences:

>Translated_393_residues
MPRISRVEYFVVEARIRGVFRIAYSAASVSRSVVVKVVLEDGTIGFGEASPSKMVTWETIDSIGSYVGLVSGQLRGLSLP
EEMGKALRVIHGYGLGFSSARAALESAILDAVGKLFGVPVYNLLGGRVAERLVTDYTVSIPDLGVARDIAGRSGRGWELY
AETIEYIVGLRSKPPEEPPIPVPSIQGFNVLKVKVGTGDPLLDAQLVLAAYEASRGRARIRVDANQAWSPKEAVRVIHRL
ENTLGDALELVEQPVPAWNLEGLRYVKERVDVPIAADESARSPVEAARVAAMGAADVINIKIAKAGGPLQAARIASLAEA
HGLQVMWGCMAETGLGIAQALHAALTSTATRYVDLDSPLFLEKDPARNSPRYRATAEGVTVEPAPGPGLATEI
>Mature_392_residues
PRISRVEYFVVEARIRGVFRIAYSAASVSRSVVVKVVLEDGTIGFGEASPSKMVTWETIDSIGSYVGLVSGQLRGLSLPE
EMGKALRVIHGYGLGFSSARAALESAILDAVGKLFGVPVYNLLGGRVAERLVTDYTVSIPDLGVARDIAGRSGRGWELYA
ETIEYIVGLRSKPPEEPPIPVPSIQGFNVLKVKVGTGDPLLDAQLVLAAYEASRGRARIRVDANQAWSPKEAVRVIHRLE
NTLGDALELVEQPVPAWNLEGLRYVKERVDVPIAADESARSPVEAARVAAMGAADVINIKIAKAGGPLQAARIASLAEAH
GLQVMWGCMAETGLGIAQALHAALTSTATRYVDLDSPLFLEKDPARNSPRYRATAEGVTVEPAPGPGLATEI

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=328, Percent_Identity=27.1341463414634, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 41882; Mature: 41750

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRISRVEYFVVEARIRGVFRIAYSAASVSRSVVVKVVLEDGTIGFGEASPSKMVTWETI
CCCCCHHHEEEEHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCEECCCCCCCCEEEHHHH
DSIGSYVGLVSGQLRGLSLPEEMGKALRVIHGYGLGFSSARAALESAILDAVGKLFGVPV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCH
YNLLGGRVAERLVTDYTVSIPDLGVARDIAGRSGRGWELYAETIEYIVGLRSKPPEEPPI
HHHHHHHHHHHHHHCEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PVPSIQGFNVLKVKVGTGDPLLDAQLVLAAYEASRGRARIRVDANQAWSPKEAVRVIHRL
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHH
ENTLGDALELVEQPVPAWNLEGLRYVKERVDVPIAADESARSPVEAARVAAMGAADVINI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCEEEEE
KIAKAGGPLQAARIASLAEAHGLQVMWGCMAETGLGIAQALHAALTSTATRYVDLDSPLF
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCCEE
LEKDPARNSPRYRATAEGVTVEPAPGPGLATEI
EECCCCCCCCCCEEECCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
PRISRVEYFVVEARIRGVFRIAYSAASVSRSVVVKVVLEDGTIGFGEASPSKMVTWETI
CCCCHHHEEEEHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCEECCCCCCCCEEEHHHH
DSIGSYVGLVSGQLRGLSLPEEMGKALRVIHGYGLGFSSARAALESAILDAVGKLFGVPV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCH
YNLLGGRVAERLVTDYTVSIPDLGVARDIAGRSGRGWELYAETIEYIVGLRSKPPEEPPI
HHHHHHHHHHHHHHCEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PVPSIQGFNVLKVKVGTGDPLLDAQLVLAAYEASRGRARIRVDANQAWSPKEAVRVIHRL
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHH
ENTLGDALELVEQPVPAWNLEGLRYVKERVDVPIAADESARSPVEAARVAAMGAADVINI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCEEEEE
KIAKAGGPLQAARIASLAEAHGLQVMWGCMAETGLGIAQALHAALTSTATRYVDLDSPLF
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCCEE
LEKDPARNSPRYRATAEGVTVEPAPGPGLATEI
EECCCCCCCCCCEEECCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]