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The map label for this gene is alaXM [H]

Identifier: 124027530

GI number: 124027530

Start: 628001

End: 628567

Strand: Reverse

Name: alaXM [H]

Synonym: Hbut_0648

Alternate gene names: 124027530

Gene position: 628567-628001 (Counterclockwise)

Preceding gene: 124027531

Following gene: 124027527

Centisome position: 37.7

GC content: 57.5

Gene sequence:

>567_bases
GTGAAGGTTACAGATGCGGTTATGGAGGCTGGCGATGCAGCACACATCGTTGCCGAGCTGCTAGAGGCGGGCTGTCAGTG
CGGTGGTTCTGGACTGGGAGCGCAGGTATCGGATGATGAAGCTCCATACGGCCAGCCATATGCTCGCAGCCGTGCTGTAT
GTGAACAGTACGAGGCAAGAGTTACCGGAGGGCATAAAGCTCCTGATATTGCATGTGATGACTTCGAGATAGGTGTGGGG
GACTGGAAGAACGCGATACTGGAGGCTGTGGAGGAGGCAAACAGGATACTACGGAGGTGCATCGAGGTCAGGGTGTACTG
GCTCCCGAGGGAGGAGGCGCTAAAGCTGCCAGGCACAGTGAAGCTGGCCAACCGCCCTCCACCGGACGTCCCGGTTCTAC
GCATTGTCGAGATACCTGGTGTCGAGTACGGGCTGACGGAGGCCCGCACGTTAGGAACACATGCAGGATGCCCCGGGATA
GAAATCGTGAAACTCGAGAGCAAGGGCAGGAGGAGAAAGAGGGTCTTACAAACTCATAGAGGGTGGGGGACAGGTAACAC
CAGCTGA

Upstream 100 bases:

>100_bases
CGACAACAATGTGTTCCTTGATGGGACAGCTTTTCATCCATGTCCAAGCGGTGGGCTTGACGCTGACTAGGGGTTACCTT
CTAACCTGGCGTGGCGACAG

Downstream 100 bases:

>100_bases
GAACGAAGCTACACCTTTCCACAGAGGCTGTTTTTACCACCGTGCTCCCGCAAACTTGGGTTGCGTGAATAGGCTTGCGT
TTATGCCCTGGCTAAAGCTT

Product: metal-dependent hydrolase

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKVTDAVMEAGDAAHIVAELLEAGCQCGGSGLGAQVSDDEAPYGQPYARSRAVCEQYEARVTGGHKAPDIACDDFEIGVG
DWKNAILEAVEEANRILRRCIEVRVYWLPREEALKLPGTVKLANRPPPDVPVLRIVEIPGVEYGLTEARTLGTHAGCPGI
EIVKLESKGRRRKRVLQTHRGWGTGNTS

Sequences:

>Translated_188_residues
MKVTDAVMEAGDAAHIVAELLEAGCQCGGSGLGAQVSDDEAPYGQPYARSRAVCEQYEARVTGGHKAPDIACDDFEIGVG
DWKNAILEAVEEANRILRRCIEVRVYWLPREEALKLPGTVKLANRPPPDVPVLRIVEIPGVEYGLTEARTLGTHAGCPGI
EIVKLESKGRRRKRVLQTHRGWGTGNTS
>Mature_188_residues
MKVTDAVMEAGDAAHIVAELLEAGCQCGGSGLGAQVSDDEAPYGQPYARSRAVCEQYEARVTGGHKAPDIACDDFEIGVG
DWKNAILEAVEEANRILRRCIEVRVYWLPREEALKLPGTVKLANRPPPDVPVLRIVEIPGVEYGLTEARTLGTHAGCPGI
EIVKLESKGRRRKRVLQTHRGWGTGNTS

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 20385; Mature: 20385

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTDAVMEAGDAAHIVAELLEAGCQCGGSGLGAQVSDDEAPYGQPYARSRAVCEQYEAR
CCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VTGGHKAPDIACDDFEIGVGDWKNAILEAVEEANRILRRCIEVRVYWLPREEALKLPGTV
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHCCCCEE
KLANRPPPDVPVLRIVEIPGVEYGLTEARTLGTHAGCPGIEIVKLESKGRRRKRVLQTHR
EECCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHC
GWGTGNTS
CCCCCCCC
>Mature Secondary Structure
MKVTDAVMEAGDAAHIVAELLEAGCQCGGSGLGAQVSDDEAPYGQPYARSRAVCEQYEAR
CCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VTGGHKAPDIACDDFEIGVGDWKNAILEAVEEANRILRRCIEVRVYWLPREEALKLPGTV
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHCCCCEE
KLANRPPPDVPVLRIVEIPGVEYGLTEARTLGTHAGCPGIEIVKLESKGRRRKRVLQTHR
EECCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHC
GWGTGNTS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]