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The map label for this gene is ypfI [C]

Identifier: 124027496

GI number: 124027496

Start: 585516

End: 587657

Strand: Reverse

Name: ypfI [C]

Synonym: Hbut_0612

Alternate gene names: 124027496

Gene position: 587657-585516 (Counterclockwise)

Preceding gene: 124027497

Following gene: 124027494

Centisome position: 35.25

GC content: 61.67

Gene sequence:

>2142_bases
ATGAAGTATAGCCACCCGGAGAGCCTTGCAAGGTTCGCCGTGGAGATACGCAACTCTGGCTACCGTGGCCTCGCTGTAGT
TCACAGCAATAGGCTCCAGTGGCTGCTGAATACTACCGCTGAAGTGCTGGACGGTAATTGTATAGCTGTGGCCCCCCAGT
CCCTTGCTGTCCCCAGGCGTTGCAGGCCCGGCTCTCCTGGCGGGTTTGAGAGGCTACTTGGCAGCGAGGCCGACTACGTT
ATCATTGCTACCGACGGCTTGCTACGACCCAACCTTGTGGCAGGCCTCGCAGGAGTAGTTTCCTCGGGAGGAATGCTGGT
CATCATGGCACCGCCGCTGCACGAGTGGAATCCAGGGCCCAGTGGCGGCGTGGGGGGCTACCGTCGCTACCTCCTCTCCT
CACTGCGCGAGGCTAGAGTGCTCCTCTGGGCCGACGCTGATACCGGTGAGGTGTACGCGTACCAGCCACCCAGGGGGAGA
GCCCACGAGTGGCTCCTCGGCCCCCGAGGCTACGAGCCCAGGTTTGGAGCGCCGAGGAGGCTCCTCGGGCTCGCCGCCAC
GCCCAGCCAGGCAGAGGCGCTGGACAGGTTCAGTCTATACCTCGACGCTGGCTATCGCAGCTTCCTGGTAACCGGCGACC
GGGGCAGAGGCAAGAGCTACCTGCTAGGCCTAGCTGTCGCCCTAGCCGTAAAGCGTGGAGCAGCTGGCGAGGCTGTGGCC
GTCGCACCTAGCCCTGCTAGCCTAGCGAGCTTCTACAAGGGACTCATTCGCGGCCTGCGAGTCCTAGGCCTACGTTACCG
CATCCGCCGAGTCGGCGGCGATGTCGTAGCTGTTACGGGGCCCTGGGGTCGTATAGCCTACGCAGCTCCCGACGATGCTA
AGCCAACCGGACTCCTTGTAGTCGACGAGGCCGCCGCCGTGGGAGTTGCAAGGCTCCGTAGACTAGCCTGGAAGAGCGGC
AGAGTGCTGGCTGCAACAACGATACACGGCTACGAGGGTAGCGGCAGAGTGTTCCAACACATGGTCGAGGATGTGCTCCC
CAAGCCCCTTGAGAGGGTTGAGCTACGTGAGCCGATACGCTACCCGCCCGGGGACCCCCTCGAGGACTGGGTTAACAGGG
TGTTCGTGCTAAAGCCCGAGCCCGACTACCCAGCTCCAGCCAGTGTTGATGAGTGCAGGGTTGAGCAGCTAGAGCCCCGC
AGCCTCGCCGAGCAGCCGGAGGCTGTTAGGAGGCTAGTCCAACTCCTAGCGCTAGCTCACTATCGCACGGAGCCCGACTA
CCTCCTCGTCATCCTCGAGTCCCGAAACCACATACTTCACGCCCTAGTCTGCGGTGGCAGTCTAGCAGCTGCGGCCGATG
TGGCACTGGAGGAGTGGGGGCTAGAGGAGCCCGCGAGGATCGCTCTTAAGATTCTGTGGCTCCAAGCAGACAGTGCGGCA
AAGGAGCTGAAGGCTGCCAGGATCGTGAGGATAGCCGTCCACCCCGAGCTGCAGCGCCGCGGCCTTGGCAGGAAGCTGCT
AAGCGCTGTCGAGGACTGGGCCCGCGGCAAAGGAGTAGACATTGTTGCCGCACTGTTTGGCCGCCACGACGTCATAGGGT
TCTGGGTTAAGACCGGCTACACACTGTTCTACGTGAGCCCCCGCTACAACAAGGCTACGGGCGAGAAGAACCTCGGCTTC
GCAAAGCCCCTAACCCCCAAGGGTGCAGAGGTGCTCACGAACGCCTCCAGGAGGGCACGGAGGAAGCTAGTTCTAGGTGC
ACATAGCACCTACCGCGACCTAGCAGCAGAAAAGACGGCTATACTCCTGAGGGCTACCCAGCCCCTCCACGATGCGCCGA
TAGTACTCGAACAGCTGGACCACAAGGCGCTCAACACCTATATGGAGGGAGTGGTCGAGCTAGAGCAGGTAATAGACTCC
GCATACCTAGCCACGGCATCAGCCCTACTGGCAGGCTGCGAAGACAGACTAGATGACCGCAGCCTAGTAGCGCTCGTGGC
GAGAATACTTCAAGGAAAACCGATCGGTGAAACAGCGGAAATCCTGGGAGCCAGCGTAGAAGAAGCACAAGCGCTCCTGC
GGGAAGCACTCAAAGCATTAATTGGGTGCAGGAGGAGTAGGACTCAACGTCGGAGCCCGTGA

Upstream 100 bases:

>100_bases
CCCGCTGTTGGGGTGATGCGGGGACCGGCAAGGCCGGTATAGTGGCCTCCCCAGCGCCGCCCCATTCTCCCATCTCCTTG
CCGAAGGGTGTGTAGCTGGC

Downstream 100 bases:

>100_bases
GGAGGCACCTCCACGCTACCCCGGCGCTGACAGTAACGGGTGAAGAATGGGCAAGGTGCCGAGGCTCTTGTCTATGTTCA
TGCTCTCCAGCCGATTCCTC

Product: P-loop ATPase fused to an acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MKYSHPESLARFAVEIRNSGYRGLAVVHSNRLQWLLNTTAEVLDGNCIAVAPQSLAVPRRCRPGSPGGFERLLGSEADYV
IIATDGLLRPNLVAGLAGVVSSGGMLVIMAPPLHEWNPGPSGGVGGYRRYLLSSLREARVLLWADADTGEVYAYQPPRGR
AHEWLLGPRGYEPRFGAPRRLLGLAATPSQAEALDRFSLYLDAGYRSFLVTGDRGRGKSYLLGLAVALAVKRGAAGEAVA
VAPSPASLASFYKGLIRGLRVLGLRYRIRRVGGDVVAVTGPWGRIAYAAPDDAKPTGLLVVDEAAAVGVARLRRLAWKSG
RVLAATTIHGYEGSGRVFQHMVEDVLPKPLERVELREPIRYPPGDPLEDWVNRVFVLKPEPDYPAPASVDECRVEQLEPR
SLAEQPEAVRRLVQLLALAHYRTEPDYLLVILESRNHILHALVCGGSLAAAADVALEEWGLEEPARIALKILWLQADSAA
KELKAARIVRIAVHPELQRRGLGRKLLSAVEDWARGKGVDIVAALFGRHDVIGFWVKTGYTLFYVSPRYNKATGEKNLGF
AKPLTPKGAEVLTNASRRARRKLVLGAHSTYRDLAAEKTAILLRATQPLHDAPIVLEQLDHKALNTYMEGVVELEQVIDS
AYLATASALLAGCEDRLDDRSLVALVARILQGKPIGETAEILGASVEEAQALLREALKALIGCRRSRTQRRSP

Sequences:

>Translated_713_residues
MKYSHPESLARFAVEIRNSGYRGLAVVHSNRLQWLLNTTAEVLDGNCIAVAPQSLAVPRRCRPGSPGGFERLLGSEADYV
IIATDGLLRPNLVAGLAGVVSSGGMLVIMAPPLHEWNPGPSGGVGGYRRYLLSSLREARVLLWADADTGEVYAYQPPRGR
AHEWLLGPRGYEPRFGAPRRLLGLAATPSQAEALDRFSLYLDAGYRSFLVTGDRGRGKSYLLGLAVALAVKRGAAGEAVA
VAPSPASLASFYKGLIRGLRVLGLRYRIRRVGGDVVAVTGPWGRIAYAAPDDAKPTGLLVVDEAAAVGVARLRRLAWKSG
RVLAATTIHGYEGSGRVFQHMVEDVLPKPLERVELREPIRYPPGDPLEDWVNRVFVLKPEPDYPAPASVDECRVEQLEPR
SLAEQPEAVRRLVQLLALAHYRTEPDYLLVILESRNHILHALVCGGSLAAAADVALEEWGLEEPARIALKILWLQADSAA
KELKAARIVRIAVHPELQRRGLGRKLLSAVEDWARGKGVDIVAALFGRHDVIGFWVKTGYTLFYVSPRYNKATGEKNLGF
AKPLTPKGAEVLTNASRRARRKLVLGAHSTYRDLAAEKTAILLRATQPLHDAPIVLEQLDHKALNTYMEGVVELEQVIDS
AYLATASALLAGCEDRLDDRSLVALVARILQGKPIGETAEILGASVEEAQALLREALKALIGCRRSRTQRRSP
>Mature_713_residues
MKYSHPESLARFAVEIRNSGYRGLAVVHSNRLQWLLNTTAEVLDGNCIAVAPQSLAVPRRCRPGSPGGFERLLGSEADYV
IIATDGLLRPNLVAGLAGVVSSGGMLVIMAPPLHEWNPGPSGGVGGYRRYLLSSLREARVLLWADADTGEVYAYQPPRGR
AHEWLLGPRGYEPRFGAPRRLLGLAATPSQAEALDRFSLYLDAGYRSFLVTGDRGRGKSYLLGLAVALAVKRGAAGEAVA
VAPSPASLASFYKGLIRGLRVLGLRYRIRRVGGDVVAVTGPWGRIAYAAPDDAKPTGLLVVDEAAAVGVARLRRLAWKSG
RVLAATTIHGYEGSGRVFQHMVEDVLPKPLERVELREPIRYPPGDPLEDWVNRVFVLKPEPDYPAPASVDECRVEQLEPR
SLAEQPEAVRRLVQLLALAHYRTEPDYLLVILESRNHILHALVCGGSLAAAADVALEEWGLEEPARIALKILWLQADSAA
KELKAARIVRIAVHPELQRRGLGRKLLSAVEDWARGKGVDIVAALFGRHDVIGFWVKTGYTLFYVSPRYNKATGEKNLGF
AKPLTPKGAEVLTNASRRARRKLVLGAHSTYRDLAAEKTAILLRATQPLHDAPIVLEQLDHKALNTYMEGVVELEQVIDS
AYLATASALLAGCEDRLDDRSLVALVARILQGKPIGETAEILGASVEEAQALLREALKALIGCRRSRTQRRSP

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP) [H]

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788818, Length=609, Percent_Identity=26.4367816091954, Blast_Score=125, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17507621, Length=403, Percent_Identity=27.0471464019851, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324197, Length=427, Percent_Identity=27.1662763466042, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640662, Length=551, Percent_Identity=24.1379310344828, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR007807 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 77652; Mature: 77652

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSHPESLARFAVEIRNSGYRGLAVVHSNRLQWLLNTTAEVLDGNCIAVAPQSLAVPRR
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEECCHHHCCCCC
CRPGSPGGFERLLGSEADYVIIATDGLLRPNLVAGLAGVVSSGGMLVIMAPPLHEWNPGP
CCCCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
SGGVGGYRRYLLSSLREARVLLWADADTGEVYAYQPPRGRAHEWLLGPRGYEPRFGAPRR
CCCCHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCHH
LLGLAATPSQAEALDRFSLYLDAGYRSFLVTGDRGRGKSYLLGLAVALAVKRGAAGEAVA
HHEECCCCHHHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEE
VAPSPASLASFYKGLIRGLRVLGLRYRIRRVGGDVVAVTGPWGRIAYAAPDDAKPTGLLV
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCEEEE
VDEAAAVGVARLRRLAWKSGRVLAATTIHGYEGSGRVFQHMVEDVLPKPLERVELREPIR
ECCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHCCCC
YPPGDPLEDWVNRVFVLKPEPDYPAPASVDECRVEQLEPRSLAEQPEAVRRLVQLLALAH
CCCCCCHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHH
YRTEPDYLLVILESRNHILHALVCGGSLAAAADVALEEWGLEEPARIALKILWLQADSAA
HCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHEEEEEEEEECCHHH
KELKAARIVRIAVHPELQRRGLGRKLLSAVEDWARGKGVDIVAALFGRHDVIGFWVKTGY
HHHHHHHEEEEEECCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCE
TLFYVSPRYNKATGEKNLGFAKPLTPKGAEVLTNASRRARRKLVLGAHSTYRDLAAEKTA
EEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHE
ILLRATQPLHDAPIVLEQLDHKALNTYMEGVVELEQVIDSAYLATASALLAGCEDRLDDR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SLVALVARILQGKPIGETAEILGASVEEAQALLREALKALIGCRRSRTQRRSP
HHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKYSHPESLARFAVEIRNSGYRGLAVVHSNRLQWLLNTTAEVLDGNCIAVAPQSLAVPRR
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEECCHHHCCCCC
CRPGSPGGFERLLGSEADYVIIATDGLLRPNLVAGLAGVVSSGGMLVIMAPPLHEWNPGP
CCCCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
SGGVGGYRRYLLSSLREARVLLWADADTGEVYAYQPPRGRAHEWLLGPRGYEPRFGAPRR
CCCCHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCHH
LLGLAATPSQAEALDRFSLYLDAGYRSFLVTGDRGRGKSYLLGLAVALAVKRGAAGEAVA
HHEECCCCHHHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEE
VAPSPASLASFYKGLIRGLRVLGLRYRIRRVGGDVVAVTGPWGRIAYAAPDDAKPTGLLV
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCEEEE
VDEAAAVGVARLRRLAWKSGRVLAATTIHGYEGSGRVFQHMVEDVLPKPLERVELREPIR
ECCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHCCCC
YPPGDPLEDWVNRVFVLKPEPDYPAPASVDECRVEQLEPRSLAEQPEAVRRLVQLLALAH
CCCCCCHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHH
YRTEPDYLLVILESRNHILHALVCGGSLAAAADVALEEWGLEEPARIALKILWLQADSAA
HCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHEEEEEEEEECCHHH
KELKAARIVRIAVHPELQRRGLGRKLLSAVEDWARGKGVDIVAALFGRHDVIGFWVKTGY
HHHHHHHEEEEEECCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCE
TLFYVSPRYNKATGEKNLGFAKPLTPKGAEVLTNASRRARRKLVLGAHSTYRDLAAEKTA
EEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHE
ILLRATQPLHDAPIVLEQLDHKALNTYMEGVVELEQVIDSAYLATASALLAGCEDRLDDR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SLVALVARILQGKPIGETAEILGASVEEAQALLREALKALIGCRRSRTQRRSP
HHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]