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The map label for this gene is ychF [C]

Identifier: 124027435

GI number: 124027435

Start: 527396

End: 528625

Strand: Direct

Name: ychF [C]

Synonym: Hbut_0550

Alternate gene names: 124027435

Gene position: 527396-528625 (Clockwise)

Preceding gene: 124027434

Following gene: 124027436

Centisome position: 31.63

GC content: 53.66

Gene sequence:

>1230_bases
TTGCCGCCACCAGAGAGGATAGCTGGCATAGTTGGTAAGACGAACGTAGGTAAGTCAACATTCTTTGCTGCTGCAACCCT
GGCAACAGTAGAGATAGGCAATAGGCCATTTGTAACACTAGAGCCGAGTACCGGTGTAGGCTATGTGCGTAAGCGCTGCA
TCCACGTGGAGCTAGGGCTTCCGCGCTGCGAACCAGCGTCGGGCTACTGTATTGATAGCTGGAGGTTCATACCGGTAAAG
CTGGTGGACATACCAGGCCTCATACCGGGCGCCCACGAAGGTAAGGGTCTTGGCAACAAGTTCCTAGACGCGATAAGACG
GGCAGACGCCATTATACTGGTTGTTGATGCTGCAGGCTCAACCGACGCAGCTGGGAACCCGGTCCCTCCTGGCACCTATG
ACCCAGTAGAGGAGGTTAGGTGGCTCGAGGTAGAACTCGAAGAGTGGATGTACGGGATAATAAAGAGCGACTGGCAACGC
TTCGCGCTCCGCGTCTCAACAACCGGGGAAAACGCTGTTGAAGCTCTTGCACAGAGACTTAGCGGGCTCAGCGTGAGGAA
AGAGCATGTCGAAGAGGCCCTAGAATATGCTGGGTTAACAGGGAAGAGCCTGACATCGTGGAGTGACGAGGATTTACGCC
GTTTCGTTAGAGGGCTGCGTCTCTCCAAGCCAATGCTTGTAGCTGCCAATAAGGCCGACATACCCGAGGCAGAGGACAAT
ATTAAGCGGATGCAGCGCGAGCTCAAGGAGTATACTGTAGTGCCCACAAGCGCAGCAGCAGAACTGGCGCTACGGCGGGC
TGCCAGGGCAGGCCTGATAAAGTACATCCCGGGCGACAGTGACTTCGAGATAATAGCTGAGGACAAGCTGTCCAGGCAGC
AGCTCAAAGCACTCGAATACATCAGGGAGCGGGTCCTCAAAAAGTGGGGCTCCACGGGAGTACAACAAGCAATAAACAAG
ACATTCCTCGAACTGCTAGACATGATAGTCGTATACCCAGTTGAAGACCCCAACAAGTACACCGACGGTAAAGGTAGGAT
ACTGCCCGACGCATATCTCGTCCCGCGCGGCACCACGGCAAGAGAACTAGCATACATGATACACACAGACCTGGGCAAAA
CATTCCTCTATGCAGTCAACGCGAGGACAAAGATGAGAATAGGCGAAGACTACATACTCCAGGACGACGATGTGATAAAA
GTCGTAGCTGCAGCTGCAAAAAAGGGTTAG

Upstream 100 bases:

>100_bases
TTATCGATATGATGTTATACATAATGCTAATGGAGTAGTCTCGCTCCACTTTTCTTCCCTACAGCTTACTCCTACCCGCT
AACCTGGGTGTGTCGCCAAA

Downstream 100 bases:

>100_bases
GCGTAGAGCTAGAGAATCCTTAGCTAGAAACAGAATATCATGTGATTTAAACATCGAGCTTTGAACCGCATCTTTAGGTT
GAGGGGGTCAGCCGGGGCGC

Product: translation-associated GTPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MPPPERIAGIVGKTNVGKSTFFAAATLATVEIGNRPFVTLEPSTGVGYVRKRCIHVELGLPRCEPASGYCIDSWRFIPVK
LVDIPGLIPGAHEGKGLGNKFLDAIRRADAIILVVDAAGSTDAAGNPVPPGTYDPVEEVRWLEVELEEWMYGIIKSDWQR
FALRVSTTGENAVEALAQRLSGLSVRKEHVEEALEYAGLTGKSLTSWSDEDLRRFVRGLRLSKPMLVAANKADIPEAEDN
IKRMQRELKEYTVVPTSAAAELALRRAARAGLIKYIPGDSDFEIIAEDKLSRQQLKALEYIRERVLKKWGSTGVQQAINK
TFLELLDMIVVYPVEDPNKYTDGKGRILPDAYLVPRGTTARELAYMIHTDLGKTFLYAVNARTKMRIGEDYILQDDDVIK
VVAAAAKKG

Sequences:

>Translated_409_residues
MPPPERIAGIVGKTNVGKSTFFAAATLATVEIGNRPFVTLEPSTGVGYVRKRCIHVELGLPRCEPASGYCIDSWRFIPVK
LVDIPGLIPGAHEGKGLGNKFLDAIRRADAIILVVDAAGSTDAAGNPVPPGTYDPVEEVRWLEVELEEWMYGIIKSDWQR
FALRVSTTGENAVEALAQRLSGLSVRKEHVEEALEYAGLTGKSLTSWSDEDLRRFVRGLRLSKPMLVAANKADIPEAEDN
IKRMQRELKEYTVVPTSAAAELALRRAARAGLIKYIPGDSDFEIIAEDKLSRQQLKALEYIRERVLKKWGSTGVQQAINK
TFLELLDMIVVYPVEDPNKYTDGKGRILPDAYLVPRGTTARELAYMIHTDLGKTFLYAVNARTKMRIGEDYILQDDDVIK
VVAAAAKKG
>Mature_408_residues
PPPERIAGIVGKTNVGKSTFFAAATLATVEIGNRPFVTLEPSTGVGYVRKRCIHVELGLPRCEPASGYCIDSWRFIPVKL
VDIPGLIPGAHEGKGLGNKFLDAIRRADAIILVVDAAGSTDAAGNPVPPGTYDPVEEVRWLEVELEEWMYGIIKSDWQRF
ALRVSTTGENAVEALAQRLSGLSVRKEHVEEALEYAGLTGKSLTSWSDEDLRRFVRGLRLSKPMLVAANKADIPEAEDNI
KRMQRELKEYTVVPTSAAAELALRRAARAGLIKYIPGDSDFEIIAEDKLSRQQLKALEYIRERVLKKWGSTGVQQAINKT
FLELLDMIVVYPVEDPNKYTDGKGRILPDAYLVPRGTTARELAYMIHTDLGKTFLYAVNARTKMRIGEDYILQDDDVIKV
VAAAAKKG

Specific function: Unknown

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=426, Percent_Identity=25.8215962441315, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1787454, Length=425, Percent_Identity=28.2352941176471, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789574, Length=141, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321649, Length=402, Percent_Identity=34.5771144278607, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6321773, Length=430, Percent_Identity=26.2790697674419, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR006073
- InterPro:   IPR013646
- InterPro:   IPR002917
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF08438 MMR_HSR1_C; PF02824 TGS [H]

EC number: NA

Molecular weight: Translated: 45210; Mature: 45078

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00905 GTP1_OBG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPERIAGIVGKTNVGKSTFFAAATLATVEIGNRPFVTLEPSTGVGYVRKRCIHVELGL
CCCHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCEEEECCCCCHHHHHHEEEEEEECC
PRCEPASGYCIDSWRFIPVKLVDIPGLIPGAHEGKGLGNKFLDAIRRADAIILVVDAAGS
CCCCCCCCCEECCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCC
TDAAGNPVPPGTYDPVEEVRWLEVELEEWMYGIIKSDWQRFALRVSTTGENAVEALAQRL
CCCCCCCCCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHH
SGLSVRKEHVEEALEYAGLTGKSLTSWSDEDLRRFVRGLRLSKPMLVAANKADIPEAEDN
HCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
IKRMQRELKEYTVVPTSAAAELALRRAARAGLIKYIPGDSDFEIIAEDKLSRQQLKALEY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEHHHHHHHHHHHHHH
IRERVLKKWGSTGVQQAINKTFLELLDMIVVYPVEDPNKYTDGKGRILPDAYLVPRGTTA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEECCCCCHH
RELAYMIHTDLGKTFLYAVNARTKMRIGEDYILQDDDVIKVVAAAAKKG
HHHHHHHHHHCCCEEEEEECCCEEEEECCCEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PPPERIAGIVGKTNVGKSTFFAAATLATVEIGNRPFVTLEPSTGVGYVRKRCIHVELGL
CCHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCEEEECCCCCHHHHHHEEEEEEECC
PRCEPASGYCIDSWRFIPVKLVDIPGLIPGAHEGKGLGNKFLDAIRRADAIILVVDAAGS
CCCCCCCCCEECCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCC
TDAAGNPVPPGTYDPVEEVRWLEVELEEWMYGIIKSDWQRFALRVSTTGENAVEALAQRL
CCCCCCCCCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHH
SGLSVRKEHVEEALEYAGLTGKSLTSWSDEDLRRFVRGLRLSKPMLVAANKADIPEAEDN
HCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
IKRMQRELKEYTVVPTSAAAELALRRAARAGLIKYIPGDSDFEIIAEDKLSRQQLKALEY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEHHHHHHHHHHHHHH
IRERVLKKWGSTGVQQAINKTFLELLDMIVVYPVEDPNKYTDGKGRILPDAYLVPRGTTA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEECCCCCHH
RELAYMIHTDLGKTFLYAVNARTKMRIGEDYILQDDDVIKVVAAAAKKG
HHHHHHHHHHCCCEEEEEECCCEEEEECCCEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]