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The map label for this gene is infB

Identifier: 124027334

GI number: 124027334

Start: 427383

End: 429221

Strand: Direct

Name: infB

Synonym: Hbut_0443

Alternate gene names: 124027334

Gene position: 427383-429221 (Clockwise)

Preceding gene: 124027317

Following gene: 124027335

Centisome position: 25.64

GC content: 52.91

Gene sequence:

>1839_bases
ATGGCAGAGCAGAAGACAAGCGGCAGGAGGCTTAGACAGCCCATTGTGGTAGTGCTAGGCCATGTAGACCATGGTAAGAC
CACGTTGCTAGACAAGATACGCGGCACAACCGTTGCCGCAAAAGAGCCGGGACTCATAACCCAGCATGTTGGCGCTAGCT
TCGTACCCGCAAGCGTTATTGAGAAGCTAGCTGAGCCGTTGAAGAAGATTATACCATTCAAGCTCATCATACCAGGCCTA
CTCTTCATAGATACCCCTGGCCACGAACTATTTGCCAACCTCCGCCGCCGTGGCGGCAGCGTAGCAGACTTCGCCATACT
AGTCGTAGATATTAATGAGGGCTTCCAGCCGCAAACCTACGAGTCCATAGAGATTCTCCGGCAGCGCCGCGTACCATTCG
TTGTTGCTGCGAACAAGATAGACAAGATACCGGGGTGGAGGGCTAACCCGGACACACCATTCCTCATATCACTCCAAAAG
CAGGCGCAGAGGGTGAGGGAAGAGCTGGAGAGGAGACTCTGGGACAATATTATCTCAAAGCTCTACGAGCTGGGCTTTCA
AGCTGACAGGTTTGACAGGATTAGGGACTTTACCAGGACCGTCGCTGTGATACCCATCTCTGCCAAGACAGGCGAAGGTA
TAGCAGAACTGCTAGCCGTGCTTGCAGGCCTTACACAGCGCTACCTGCAGCATCGTCTACGCTTTGCTGAGGGTCCCGCT
AAGGGTGTCATCCTCGAGATTCGTGAGCAGCCAGGCCTGGGTACTGCAGCAGACGTCGTGATATACGACGGCGTGCTCCG
GAAAGGCGACATAATCGTAACCGGTGGACTTAACGGCCCGGTAATAACACATGTAAGGGCGCTATTGATGCCTAAACCGC
TCCAGGAGATTAGGGTTGCCAAGCGCGAGCTAGAGCCCGTAGAGGAGGTCTACGCGGCTGCAGGTGTTAGGATTGTTGCG
CCAGGTCTTGAGGAGGCGATTGCAGGTGCACCCGTCTTCGTTGCTAGGGACGAGGGGGAAGCTAAGCAGCTAGCAGAGAA
GGTTAGGAGGGAGATAGAAGCTCTGAGGATTAAAACTGAAGCTGAGGGCGTAATTGTCAAGGCCGATACGCTTGGCAGCC
TTGAAGCGATGATAGAGGCTCTGAGGAAACGTGACATACCCATACGCTACGCCGATGTAGGCCCCGTAGCTAAGCGCGAC
GTGATCGAAGCAGTTGCCAGCAGGGAACTCAACAAGTTCTATGGCGTCATACTAGCATTTAACGTGAAGGTACTACCAGA
GGCTGAGGAGGAGGCTAAAAAGCACGGCATAACAATCTTCACCAACAACGTGATCTACCGGCTGCTAGAGGACTTTGAGA
AATGGTATAAGGAGCAGGTAGAGGCTGAGCGGAGGAAGGAGCTTGAAAAGCTTGTGAGACCAGGCAAGATAAGACTCCTG
CCAGGCTATGTGTTTAGGAGGAGTAATCCTGCAATAGTCGGTGTCGAGGTCCTGGGCGGTGTGATAAAGCCCGGCTACCC
CTTGATGAGGGAGGATGGAAAGAGAATCGGCACCATTCACCAGATACAGGACAAGGGCAAGGTTATACATGAGGCTCGTG
CTGGCATGGCAGTTGCTATATCGATACGTGGCCACGTGATGGTCGGTAGACATATCGACGAGGGAGATGTACTTTATACG
GATATACCGGAGCAACACGCAGTACTGTGGCTAACGAAGTTTAAGAGCGAGCTAACAGATGACGAGATGGTGGTGTTGAA
GGAGATAATCAAGATTAAAAGGAAGCAAAATCCCGCCTATGCTGTCGTCCTGGGTAAGCCTCAGGGAGCCAAGCCTTGA

Upstream 100 bases:

>100_bases
TAATTAATTCTCGGCTCTACCTTACTGCCCCGGCTTCCTAGCCATGCCCGTTATAGGGGGCTACTCGTCCTTTACGCCTT
GTGGGGGGTGAGAGTATAGC

Downstream 100 bases:

>100_bases
GATGCACAGATGGAGAAACTCCTTTTTACGAAGCACTTCCCGGCACTAGGCAGGTAATAGAGCTAGCTAAGCTTTACCGG
GAAAGGCTGGATGAGAAGGG

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MAEQKTSGRRLRQPIVVVLGHVDHGKTTLLDKIRGTTVAAKEPGLITQHVGASFVPASVIEKLAEPLKKIIPFKLIIPGL
LFIDTPGHELFANLRRRGGSVADFAILVVDINEGFQPQTYESIEILRQRRVPFVVAANKIDKIPGWRANPDTPFLISLQK
QAQRVREELERRLWDNIISKLYELGFQADRFDRIRDFTRTVAVIPISAKTGEGIAELLAVLAGLTQRYLQHRLRFAEGPA
KGVILEIREQPGLGTAADVVIYDGVLRKGDIIVTGGLNGPVITHVRALLMPKPLQEIRVAKRELEPVEEVYAAAGVRIVA
PGLEEAIAGAPVFVARDEGEAKQLAEKVRREIEALRIKTEAEGVIVKADTLGSLEAMIEALRKRDIPIRYADVGPVAKRD
VIEAVASRELNKFYGVILAFNVKVLPEAEEEAKKHGITIFTNNVIYRLLEDFEKWYKEQVEAERRKELEKLVRPGKIRLL
PGYVFRRSNPAIVGVEVLGGVIKPGYPLMREDGKRIGTIHQIQDKGKVIHEARAGMAVAISIRGHVMVGRHIDEGDVLYT
DIPEQHAVLWLTKFKSELTDDEMVVLKEIIKIKRKQNPAYAVVLGKPQGAKP

Sequences:

>Translated_612_residues
MAEQKTSGRRLRQPIVVVLGHVDHGKTTLLDKIRGTTVAAKEPGLITQHVGASFVPASVIEKLAEPLKKIIPFKLIIPGL
LFIDTPGHELFANLRRRGGSVADFAILVVDINEGFQPQTYESIEILRQRRVPFVVAANKIDKIPGWRANPDTPFLISLQK
QAQRVREELERRLWDNIISKLYELGFQADRFDRIRDFTRTVAVIPISAKTGEGIAELLAVLAGLTQRYLQHRLRFAEGPA
KGVILEIREQPGLGTAADVVIYDGVLRKGDIIVTGGLNGPVITHVRALLMPKPLQEIRVAKRELEPVEEVYAAAGVRIVA
PGLEEAIAGAPVFVARDEGEAKQLAEKVRREIEALRIKTEAEGVIVKADTLGSLEAMIEALRKRDIPIRYADVGPVAKRD
VIEAVASRELNKFYGVILAFNVKVLPEAEEEAKKHGITIFTNNVIYRLLEDFEKWYKEQVEAERRKELEKLVRPGKIRLL
PGYVFRRSNPAIVGVEVLGGVIKPGYPLMREDGKRIGTIHQIQDKGKVIHEARAGMAVAISIRGHVMVGRHIDEGDVLYT
DIPEQHAVLWLTKFKSELTDDEMVVLKEIIKIKRKQNPAYAVVLGKPQGAKP
>Mature_611_residues
AEQKTSGRRLRQPIVVVLGHVDHGKTTLLDKIRGTTVAAKEPGLITQHVGASFVPASVIEKLAEPLKKIIPFKLIIPGLL
FIDTPGHELFANLRRRGGSVADFAILVVDINEGFQPQTYESIEILRQRRVPFVVAANKIDKIPGWRANPDTPFLISLQKQ
AQRVREELERRLWDNIISKLYELGFQADRFDRIRDFTRTVAVIPISAKTGEGIAELLAVLAGLTQRYLQHRLRFAEGPAK
GVILEIREQPGLGTAADVVIYDGVLRKGDIIVTGGLNGPVITHVRALLMPKPLQEIRVAKRELEPVEEVYAAAGVRIVAP
GLEEAIAGAPVFVARDEGEAKQLAEKVRREIEALRIKTEAEGVIVKADTLGSLEAMIEALRKRDIPIRYADVGPVAKRDV
IEAVASRELNKFYGVILAFNVKVLPEAEEEAKKHGITIFTNNVIYRLLEDFEKWYKEQVEAERRKELEKLVRPGKIRLLP
GYVFRRSNPAIVGVEVLGGVIKPGYPLMREDGKRIGTIHQIQDKGKVIHEARAGMAVAISIRGHVMVGRHIDEGDVLYTD
IPEQHAVLWLTKFKSELTDDEMVVLKEIIKIKRKQNPAYAVVLGKPQGAKP

Specific function: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI84043963, Length=593, Percent_Identity=38.4485666104553, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI53729339, Length=264, Percent_Identity=34.0909090909091, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI53729337, Length=264, Percent_Identity=34.0909090909091, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1789559, Length=578, Percent_Identity=28.8927335640138, Blast_Score=145, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI212656558, Length=606, Percent_Identity=38.4488448844884, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71994658, Length=485, Percent_Identity=28.8659793814433, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319282, Length=571, Percent_Identity=39.4045534150613, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324550, Length=529, Percent_Identity=27.9773156899811, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24656849, Length=604, Percent_Identity=39.4039735099338, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI28572034, Length=550, Percent_Identity=30, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI160714833, Length=87, Percent_Identity=47.1264367816092, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2P_HYPBU (A2BJZ8)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012654.1
- ProteinModelPortal:   A2BJZ8
- SMR:   A2BJZ8
- STRING:   A2BJZ8
- GeneID:   4782603
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0443
- eggNOG:   arNOG04413
- HOGENOM:   HBG635083
- OMA:   MGLSQRY
- PhylomeDB:   A2BJZ8
- ProtClustDB:   PRK04004
- BioCyc:   HBUT415426:HBUT_0443-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00100_A
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004544
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00491
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 68180; Mature: 68048

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQKTSGRRLRQPIVVVLGHVDHGKTTLLDKIRGTTVAAKEPGLITQHVGASFVPASVI
CCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCHHHCCCCCCHHHHH
EKLAEPLKKIIPFKLIIPGLLFIDTPGHELFANLRRRGGSVADFAILVVDINEGFQPQTY
HHHHHHHHHHCCHHHHHCCEEEEECCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHH
ESIEILRQRRVPFVVAANKIDKIPGWRANPDTPFLISLQKQAQRVREELERRLWDNIISK
HHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
LYELGFQADRFDRIRDFTRTVAVIPISAKTGEGIAELLAVLAGLTQRYLQHRLRFAEGPA
HHHCCCCCHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KGVILEIREQPGLGTAADVVIYDGVLRKGDIIVTGGLNGPVITHVRALLMPKPLQEIRVA
CCEEEEEECCCCCCCHHHHEEECCEECCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
KRELEPVEEVYAAAGVRIVAPGLEEAIAGAPVFVARDEGEAKQLAEKVRREIEALRIKTE
HHHCCHHHHHHHHCCCEEECCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEEC
AEGVIVKADTLGSLEAMIEALRKRDIPIRYADVGPVAKRDVIEAVASRELNKFYGVILAF
CCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEE
NVKVLPEAEEEAKKHGITIFTNNVIYRLLEDFEKWYKEQVEAERRKELEKLVRPGKIRLL
EEEECCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
PGYVFRRSNPAIVGVEVLGGVIKPGYPLMREDGKRIGTIHQIQDKGKVIHEARAGMAVAI
CCEEEECCCCCEEEHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
SIRGHVMVGRHIDEGDVLYTDIPEQHAVLWLTKFKSELTDDEMVVLKEIIKIKRKQNPAY
EEECEEEECCCCCCCCEEEECCCCCHHEEHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCE
AVVLGKPQGAKP
EEEEECCCCCCC
>Mature Secondary Structure 
AEQKTSGRRLRQPIVVVLGHVDHGKTTLLDKIRGTTVAAKEPGLITQHVGASFVPASVI
CCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCHHHCCCCCCHHHHH
EKLAEPLKKIIPFKLIIPGLLFIDTPGHELFANLRRRGGSVADFAILVVDINEGFQPQTY
HHHHHHHHHHCCHHHHHCCEEEEECCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHH
ESIEILRQRRVPFVVAANKIDKIPGWRANPDTPFLISLQKQAQRVREELERRLWDNIISK
HHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
LYELGFQADRFDRIRDFTRTVAVIPISAKTGEGIAELLAVLAGLTQRYLQHRLRFAEGPA
HHHCCCCCHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KGVILEIREQPGLGTAADVVIYDGVLRKGDIIVTGGLNGPVITHVRALLMPKPLQEIRVA
CCEEEEEECCCCCCCHHHHEEECCEECCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
KRELEPVEEVYAAAGVRIVAPGLEEAIAGAPVFVARDEGEAKQLAEKVRREIEALRIKTE
HHHCCHHHHHHHHCCCEEECCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEEC
AEGVIVKADTLGSLEAMIEALRKRDIPIRYADVGPVAKRDVIEAVASRELNKFYGVILAF
CCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEE
NVKVLPEAEEEAKKHGITIFTNNVIYRLLEDFEKWYKEQVEAERRKELEKLVRPGKIRLL
EEEECCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
PGYVFRRSNPAIVGVEVLGGVIKPGYPLMREDGKRIGTIHQIQDKGKVIHEARAGMAVAI
CCEEEECCCCCEEEHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
SIRGHVMVGRHIDEGDVLYTDIPEQHAVLWLTKFKSELTDDEMVVLKEIIKIKRKQNPAY
EEECEEEECCCCCCCCEEEECCCCCHHEEHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCE
AVVLGKPQGAKP
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA