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The map label for this gene is nth [H]

Identifier: 124027163

GI number: 124027163

Start: 252765

End: 253493

Strand: Direct

Name: nth [H]

Synonym: Hbut_0266

Alternate gene names: 124027163

Gene position: 252765-253493 (Clockwise)

Preceding gene: 124027161

Following gene: 124027164

Centisome position: 15.16

GC content: 62.83

Gene sequence:

>729_bases
ATGGCTGGGTGCAGTATTGCCAACTATGCTTCGGGCTGCGAGGTTTTCGAGAGGCTCCAGCGTAGTCTCCGTGTGGACTG
GCGCGACTACGTGGCTCTGGTCGCGTTCGAGTATGATGCGCGGGAGCACCCGTTCGCTGTGCTTGCAGGGATCATACTGA
GCCAGAATACTAGCGACAGGAACTCTATCCGGGCCTACCTGCAGCTCCGCGAGATGGTTGGCGTATCCCCGGAGGCCGTG
CTTTCGGCGCCGGAGGACAGGCTTATCGAGGCTATTAGGCCGGCTGGGCTGGCAAGGCAGAAGGCTAGGGCGCTCCGGGA
GGCTGCCCGCCGCATCCTCGAGGCTGGCGGCGAGAAGGTACTCCTGGAGATGCCCTGGAGGGAGCTGAGAGAGTTCCTCC
TATCGATACCCGGTGTCGGCAAGAAGACTGCTGACGTGTTTCTCCAGCTTGTCCGGAAAGCGCCAGTCTTCGCGGTGGAT
ACTCATGCCGCACGTATCGCGAAGAGATGGGGACTCGTCGGCGAGAAGGCGGGCTACGACGAGACCTCACGGGCCCTCCT
AGAGTTCTTCGGGCCAGAGCGCAGCGAGAATGCACACCGGCTCCTCATAGCGCTGGGTAGAACCTACTGCCGAGCACGCA
ACCCCCGCTGCGACGTGTGCCCTCTACGCGACATATGCCCCTACCCCCGCGGCTGCAGCACGGGGAGGAATGATGGAGGA
GAGGGCTAG

Upstream 100 bases:

>100_bases
AGCCCTATCTTCGGGAAAGATGTATTATCCACAACATCCACGTCTAGGGCTAGCGTTATGGATGTACTTGGACTGTATAG
CTGTGAGGGTGTAGGAGATT

Downstream 100 bases:

>100_bases
GCTCTACCCGAACGAGTGCGTAAAGCGCGTCGTAGCCTTCATACCGGAGGGCCACATGCACGCCAGGCTAATAATCGAAC
TCTGCGACCAGAAGATAATA

Product: EndoIII-related endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MAGCSIANYASGCEVFERLQRSLRVDWRDYVALVAFEYDAREHPFAVLAGIILSQNTSDRNSIRAYLQLREMVGVSPEAV
LSAPEDRLIEAIRPAGLARQKARALREAARRILEAGGEKVLLEMPWRELREFLLSIPGVGKKTADVFLQLVRKAPVFAVD
THAARIAKRWGLVGEKAGYDETSRALLEFFGPERSENAHRLLIALGRTYCRARNPRCDVCPLRDICPYPRGCSTGRNDGG
EG

Sequences:

>Translated_242_residues
MAGCSIANYASGCEVFERLQRSLRVDWRDYVALVAFEYDAREHPFAVLAGIILSQNTSDRNSIRAYLQLREMVGVSPEAV
LSAPEDRLIEAIRPAGLARQKARALREAARRILEAGGEKVLLEMPWRELREFLLSIPGVGKKTADVFLQLVRKAPVFAVD
THAARIAKRWGLVGEKAGYDETSRALLEFFGPERSENAHRLLIALGRTYCRARNPRCDVCPLRDICPYPRGCSTGRNDGG
EG
>Mature_241_residues
AGCSIANYASGCEVFERLQRSLRVDWRDYVALVAFEYDAREHPFAVLAGIILSQNTSDRNSIRAYLQLREMVGVSPEAVL
SAPEDRLIEAIRPAGLARQKARALREAARRILEAGGEKVLLEMPWRELREFLLSIPGVGKKTADVFLQLVRKAPVFAVDT
HAARIAKRWGLVGEKAGYDETSRALLEFFGPERSENAHRLLIALGRTYCRARNPRCDVCPLRDICPYPRGCSTGRNDGGE
G

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=200, Percent_Identity=27.5, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787920, Length=185, Percent_Identity=32.4324324324324, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554540, Length=201, Percent_Identity=28.3582089552239, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324530, Length=201, Percent_Identity=26.3681592039801, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550361, Length=195, Percent_Identity=27.1794871794872, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26986; Mature: 26855

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGCSIANYASGCEVFERLQRSLRVDWRDYVALVAFEYDAREHPFAVLAGIILSQNTSDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCH
NSIRAYLQLREMVGVSPEAVLSAPEDRLIEAIRPAGLARQKARALREAARRILEAGGEKV
HHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEE
LLEMPWRELREFLLSIPGVGKKTADVFLQLVRKAPVFAVDTHAARIAKRWGLVGEKAGYD
EEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCC
ETSRALLEFFGPERSENAHRLLIALGRTYCRARNPRCDVCPLRDICPYPRGCSTGRNDGG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
EG
CC
>Mature Secondary Structure 
AGCSIANYASGCEVFERLQRSLRVDWRDYVALVAFEYDAREHPFAVLAGIILSQNTSDR
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCH
NSIRAYLQLREMVGVSPEAVLSAPEDRLIEAIRPAGLARQKARALREAARRILEAGGEKV
HHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEE
LLEMPWRELREFLLSIPGVGKKTADVFLQLVRKAPVFAVDTHAARIAKRWGLVGEKAGYD
EEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCC
ETSRALLEFFGPERSENAHRLLIALGRTYCRARNPRCDVCPLRDICPYPRGCSTGRNDGG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]